Difference between revisions of "Team:JNFLS/Design"

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<h1>Design</h1>
 
<h1>Design</h1>
 
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
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In the very beginning of our project, we brainstormed about the detection measures for HCV. The current detection method used in all blood centers in Shandong province is ELISA-based HCV antibody detection, which caused the awkward situation that not even a single positive result was gained in the last decade. HCV antibody detection will be the past, but what the future will be? ELISA-based antigen detection? No, the detection must be sufficiently sensitive, what if the amount of virus in the blood is too low? Nucleic acid detection? No, it shares the same drawback as the previous method; also, RNA is way easier to be degraded. In the end, the works of Zhang et al and Zhou inspired us. We eventually designed a biosensor based on aptamer and rolling circle amplification.  
 
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<img src="https://static.igem.org/mediawiki/2018/b/b3/T--JNFLS--ban.jpg"style="width:70%">
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<img src="https://static.igem.org/mediawiki/2018/7/7e/T--JNFLS--liu.jpg"style="width:80%">
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This page is different to the "Applied Design Award" page. Please see the <a href="https://2018.igem.org/Team:JNFLS/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
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<img src="https://static.igem.org/mediawiki/2018/2/25/T--JNFLS--han.jpg"style="width:30%">
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<img src="https://static.igem.org/mediawiki/2018/d/d7/T--JNFLS--tian.jpg"style="width:80%">
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<img src="https://static.igem.org/mediawiki/2018/7/73/T--JNFLS--xian.jpg"styel="width:80%">
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<img src="https://static.igem.org/mediawiki/2018/a/a0/T--JNFLS--baobao.jpg"styel="width:1%">
 
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<li>Li Shengtao. Purification of expression of HCV "truncated" core protein (HCV Core125) and preparation of antibodies [D]. Kunming university of technology,2012.</li>
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<li>Wu Xianbo. Cloning, expression and purification and antigenicity analysis of HCV core antigen gene fragments [D]. First military medical university of the people's liberation army,2003.</li>
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<li>Zhang Songbai, Zheng Liying, Hu xia, Shen Guangyu, Liu Xuewen, Shen Guoli, Yu Ruqin. High sensitivity detection of thrombin by fluorescent adaptor sensor based on competitive trigger rolling ring amplification [J]. Analytical chemistry,2015,43(11):1688-1694.</li>
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<li>Zhou hui. Study on nucleic acid aptamer sensor based on signal amplification triggered by competitive mechanism [D]. Hunan university,2009.</li>
  
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<h3>What should this page contain?</h3>
 
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<li>Explanation of the engineering principles your team used in your design</li>
 
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<li>Discussion of the design iterations your team went through</li>
 
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<li>Experimental plan to test your designs</li>
 
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<h3>Inspiration</h3>
 
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<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
 
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<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
 
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<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
 
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Revision as of 13:12, 18 September 2018

Design

In the very beginning of our project, we brainstormed about the detection measures for HCV. The current detection method used in all blood centers in Shandong province is ELISA-based HCV antibody detection, which caused the awkward situation that not even a single positive result was gained in the last decade. HCV antibody detection will be the past, but what the future will be? ELISA-based antigen detection? No, the detection must be sufficiently sensitive, what if the amount of virus in the blood is too low? Nucleic acid detection? No, it shares the same drawback as the previous method; also, RNA is way easier to be degraded. In the end, the works of Zhang et al and Zhou inspired us. We eventually designed a biosensor based on aptamer and rolling circle amplification.

  1. Li Shengtao. Purification of expression of HCV "truncated" core protein (HCV Core125) and preparation of antibodies [D]. Kunming university of technology,2012.
  2. Wu Xianbo. Cloning, expression and purification and antigenicity analysis of HCV core antigen gene fragments [D]. First military medical university of the people's liberation army,2003.
  3. Zhang Songbai, Zheng Liying, Hu xia, Shen Guangyu, Liu Xuewen, Shen Guoli, Yu Ruqin. High sensitivity detection of thrombin by fluorescent adaptor sensor based on competitive trigger rolling ring amplification [J]. Analytical chemistry,2015,43(11):1688-1694.
  4. Zhou hui. Study on nucleic acid aptamer sensor based on signal amplification triggered by competitive mechanism [D]. Hunan university,2009.