Difference between revisions of "Team:OUC-China/Attributions"

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<h2 class="major"><span>Abstract</span></h2>
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<h2 class="major"><span>MEMBER'S ATTRIBUTION</span></h2>
<p align="justify">This year, we design a toolkit focused on post-transcriptional regulation, which is composed of a RNA endoribonuclease (Csy4) and a RNA module named miniToe. Csy4 (Csy6f), a member of CRISPR family, recognizes a specific 22nt hairpin. The RNA module was constructed by inserting the 22nt Csy4 recognition site between a RBS and cis-repressive RNA element, which can be specifically cleaved upon Csy4 expression, so the RBS is usually masked. Cleaved at the specific recognition site, it can release the masked RBS, thus endowing the programming of gene expression in the translation level with higher feasibility. We want to use one system to achieve diverse expression of target gene. So we further design four Csy4 mutants and five miniToe mutants. The whole system including five Csy4s and six miniToes is called miniToe family. By combining each Csy4 and hairpin, we can achieve different expression level of the target proteins in a polycistron.</p>
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<p align="justify">From the beginning of 2018, OUC-China have brand new members for this competition season. With the help of experienced team leaders before, we build a team consists of 8 undergraduate students from different majors and two grades. And by sharing happiness and woe this season, now we become close friends and have the tacit understanding. All the members in 2018 OUC-China devoted the best of themselves to develop the project. At the same time, we would have gone nowhere without the help from many other people from our school, other institutions and the society. We sincerely appreciate all the help!
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Yunqian Zhang, captain of OUC-China iGEM 2018 and general affair supervisor, was responsible for designing our project and experiment. She designed and checked all the circuit as well as the primers used in PCR and part standardization. She provided basic experimental scheme for lab administrator. She was responsible for construct and test by microplate reader of polycistron circuit. Also she arranged journals for all people to CCiC and Boston.
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Yanju Chen, vice-captain of our team, pushed the preceding of human practice and communication with other teams. She keep connection with all the professors who helped us. She is also responsible for submitting parts and document the experimental characterization. Also she arranged journals for all people to Beijing.
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Jiacheng Huang, vice-captain of our team. He selected all the csy4 mutants and hairpin mutants to meet our goal. He was in charge of modeling and excavated deeply in theory who also is one of wiki designer.
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Qinbo Qiao, lab administrator and arranged the journal to get visa. Her major work was part standardization and extracted a mount of DNA.
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Xudong Liu, lab administrator, was in charge of construct circuits by using 3A assembly and detected the expression of proteins. Also he was responsible for the measurement of the growth rate of E.coli, providing experimental limits for model. He changed our experimental scheme with need at any moment.
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Qingjie Li, the financial manager of our team and designer for our Video Game——Csy4 Go! She was also responsible for our motA test system. She designed the experiment for this system and finished all the lab work for this part by herself. She helped us with a lot of biological order for biotechnology companies.
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Kai Sun, the web designer and art designer who established the structure of our wiki, poster, team uniform and many other designing works. At the same time, he also take the task of Interlab measurement and primers design.
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Anyi Li, was responsible for primers design and plan for human practice. At the same time, she test the second system which contain many Csy4 mutants and hairpin mutants by flow cytometry for 6 times. And she writed our notebook and took part in some lab work.</p>
 
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Revision as of 08:13, 20 September 2018

Team OUC-China: Main

MEMBER'S ATTRIBUTION

From the beginning of 2018, OUC-China have brand new members for this competition season. With the help of experienced team leaders before, we build a team consists of 8 undergraduate students from different majors and two grades. And by sharing happiness and woe this season, now we become close friends and have the tacit understanding. All the members in 2018 OUC-China devoted the best of themselves to develop the project. At the same time, we would have gone nowhere without the help from many other people from our school, other institutions and the society. We sincerely appreciate all the help!   Yunqian Zhang, captain of OUC-China iGEM 2018 and general affair supervisor, was responsible for designing our project and experiment. She designed and checked all the circuit as well as the primers used in PCR and part standardization. She provided basic experimental scheme for lab administrator. She was responsible for construct and test by microplate reader of polycistron circuit. Also she arranged journals for all people to CCiC and Boston. Yanju Chen, vice-captain of our team, pushed the preceding of human practice and communication with other teams. She keep connection with all the professors who helped us. She is also responsible for submitting parts and document the experimental characterization. Also she arranged journals for all people to Beijing. Jiacheng Huang, vice-captain of our team. He selected all the csy4 mutants and hairpin mutants to meet our goal. He was in charge of modeling and excavated deeply in theory who also is one of wiki designer. Qinbo Qiao, lab administrator and arranged the journal to get visa. Her major work was part standardization and extracted a mount of DNA. Xudong Liu, lab administrator, was in charge of construct circuits by using 3A assembly and detected the expression of proteins. Also he was responsible for the measurement of the growth rate of E.coli, providing experimental limits for model. He changed our experimental scheme with need at any moment. Qingjie Li, the financial manager of our team and designer for our Video Game——Csy4 Go! She was also responsible for our motA test system. She designed the experiment for this system and finished all the lab work for this part by herself. She helped us with a lot of biological order for biotechnology companies. Kai Sun, the web designer and art designer who established the structure of our wiki, poster, team uniform and many other designing works. At the same time, he also take the task of Interlab measurement and primers design. Anyi Li, was responsible for primers design and plan for human practice. At the same time, she test the second system which contain many Csy4 mutants and hairpin mutants by flow cytometry for 6 times. And she writed our notebook and took part in some lab work.

Team Members

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