Difference between revisions of "Team:OUC-China/Parts"

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<h2 class="major"><span>MEMBER'S ATTRIBUTION</span></h2>
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<h2 class="major"><span>Silver part: Designing a new part</span></h2>
 
 
 
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&emsp;&emsp;From the beginning of 2018, OUC-China have brand new members for this competition season. With the help of experienced team leaders before, we build a team consists of 8 undergraduate students from different majors and two grades. And by sharing happiness and woe this season, now we become close friends and have the tacit understanding. All the members in 2018 OUC-China devoted the best of themselves to develop the project. At the same time, we would have gone nowhere without the help from many other people from our school, other institutions and the society. We sincerely appreciate all the help!
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&emsp;&emsp;This year, we design a basic part called miniToe, which consists of RBS, Csy4 recognition site and cis-repressive RNA element. As a regulatory element, it can be specifically recognized and cleaved by Csy4 to regulate the expression of its downstream genes from the RNA level. MiniToe is the core of our project design, is RFC10  compatible  and works as expected. We have documented its experimental characterization on Part's Main Page on the Registry and submitted the sample to the Registry. Certainly, this part is different from the new part documented for Gold#2. See the page for more details: http://parts.igem.org/Part:BBa_K2615020.
 
 
 
 
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<br />&emsp;&emsp;Yunqian Zhang, captain of OUC-China iGEM 2018 and general affair supervisor, was responsible for designing our project and experiment. She designed and checked all the circuit as well as the primers used in PCR and part standardization. She provided basic experimental scheme for lab administrator. She was responsible for construct and test by microplate reader of polycistron circuit. Also she arranged journals for all people to CCiC and Boston.
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<br />&emsp;&emsp;Yanju Chen, vice-captain of our team, pushed the preceding of human practice and communication with other teams. She keep connection with all the professors who helped us. She is also responsible for submitting parts and document the experimental characterization. Also she arranged journals for all people to Beijing.
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<br />&emsp;&emsp;Jiacheng Huang, vice-captain of our team. He selected all the csy4 mutants and hairpin mutants to meet our goal. He was in charge of modeling and excavated deeply in theory who also is one of wiki designer.
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<br />&emsp;&emsp;Qinbo Qiao, lab administrator and arranged the journal to get visa. Her major work was part standardization and extracted a mount of DNA.
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<br />&emsp;&emsp;Xudong Liu, lab administrator, was in charge of construct circuits by using 3A assembly and detected the expression of proteins. Also he was responsible for the measurement of the growth rate of E.coli, providing experimental limits for model. He changed our experimental scheme with need at any moment.
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<br />&emsp;&emsp;Qingjie Li, the financial manager of our team and designer for our Video Game——Csy4 Go! She was also responsible for our motA test system. She designed the experiment for this system and finished all the lab work for this part by herself. She helped us with a lot of biological order for biotechnology companies.
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<br />&emsp;&emsp;Kai Sun, the web designer and art designer who established the structure of our wiki, poster, team uniform and many other designing works. At the same time, he also take the task of Interlab measurement and primers design.
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<br />&emsp;&emsp;Anyi Li, was responsible for primers design and plan for human practice. At the same time, she test the second system which contain many Csy4 mutants and hairpin mutants by flow cytometry for 6 times. And she writed our notebook and took part in some lab work.</p>
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<h2 class="major"><span>Acknowledgement for support</span></h2>
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<h2 class="major"><span>Gold parts: Improving an existing part</span></h2>
 
 
 
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&emsp;&emsp;Every member in 2017 OUC-China iGEM gave us practical suggestions.
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&emsp;&emsp;Improving an existing Part
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<br />&emsp;&emsp;Yang Liu, the Captain of OUC-China 2011 and Li Kang, the Captain of OUC-China 2012 helped improve the synthetic conception of our project. 
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<br />&emsp;&emsp;Standardization and building up on existing parts are the fundaments of iGEM. We have created FOUR new BioBrick Part (BBa_K2615004, BBa_K2615005, BBa_K2615006, BBa_K2615007) that has a functional improvement upon an existing BioBrick Part (BBa_K1062004). The sequences of four new parts and existing part are different, and the new parts are changed by point mutation. We have showed experiments with both parts to demonstrate this improvement.
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<br />&emsp;&emsp;Our PI Prof. Chenguang Liu and Prof. Xingguo Liang offered us constructive advice over our project and gave us long-term technical consulting support through the procedure.
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<br />&emsp;&emsp;See the pages below for details:
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<br />&emsp;&emsp;Prof Chunbo Lou and Pei Du, from Institute of Microbiology, provided us with original idea by their work and also taught us with some details about experiment and lab work.
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<br />&emsp;&emsp;The existing part
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<br />&emsp;&emsp;Our PI Prof.Xingguo Liang offered us experimental lab.
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<br />&emsp;&emsp;http://parts.igem.org/Part:BBa_K1062004:Experience
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<br />&emsp;&emsp;Our PI Prof.Zhe Chi helped us with gene synthesis and financial problems, at the same time, he also provided us with unique advice about project.
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<br />&emsp;&emsp;The four improved parts
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<br />&emsp;&emsp;Prof. Xiaohua Zhang, from College of Marine Life, Ocean University of China, provided us instrument (Thermo Scientific NanoDrop) to measure the concentration of nucleic acid. 
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<br />&emsp;&emsp;http://parts.igem.org/Part:BBa_K2615004
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<br />&emsp;&emsp;Prof Mingzhuang Zhu and Wei Yang provided us the flow cytometry which was really helpful for our second system. </p>
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<br />&emsp;&emsp;http://parts.igem.org/Part:BBa_K2615005
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<br />&emsp;&emsp;http://parts.igem.org/Part:BBa_K2615006
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<br />&emsp;&emsp;http://parts.igem.org/Part:BBa_K2615007
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<groupparts>iGEM18 OUC-China</groupparts>
 
 
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<groupparts>iGEM18 OUC-China</groupparts>

Revision as of 11:25, 21 September 2018

Team OUC-China: Main
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Silver part: Designing a new part

  This year, we design a basic part called miniToe, which consists of RBS, Csy4 recognition site and cis-repressive RNA element. As a regulatory element, it can be specifically recognized and cleaved by Csy4 to regulate the expression of its downstream genes from the RNA level. MiniToe is the core of our project design, is RFC10 compatible and works as expected. We have documented its experimental characterization on Part's Main Page on the Registry and submitted the sample to the Registry. Certainly, this part is different from the new part documented for Gold#2. See the page for more details: http://parts.igem.org/Part:BBa_K2615020.  

Gold parts: Improving an existing part

  Improving an existing Part
  Standardization and building up on existing parts are the fundaments of iGEM. We have created FOUR new BioBrick Part (BBa_K2615004, BBa_K2615005, BBa_K2615006, BBa_K2615007) that has a functional improvement upon an existing BioBrick Part (BBa_K1062004). The sequences of four new parts and existing part are different, and the new parts are changed by point mutation. We have showed experiments with both parts to demonstrate this improvement.
  See the pages below for details:
  The existing part
  http://parts.igem.org/Part:BBa_K1062004:Experience
  The four improved parts
  http://parts.igem.org/Part:BBa_K2615004
  http://parts.igem.org/Part:BBa_K2615005
  http://parts.igem.org/Part:BBa_K2615006
  http://parts.igem.org/Part:BBa_K2615007

Contact Us : oucigem@163.com | ©2018 OUC IGEM.All Rights Reserved. | Based On Dreamweaver

<groupparts>iGEM18 OUC-China</groupparts>