|
|
| Line 1: |
Line 1: |
| − | {{Tokyo_Tech/css/reset}} | + | {{Tokyo_Tech/css/template.css}} |
| − | {{Tokyo_Tech/css/main}}
| + | |
| − | <!DOCTYPE html>
| + | |
| | <html> | | <html> |
| − | <title>Coli Sapiens</title> | + | <head> |
| − | <meta charset="UTF-8">
| + | |
| − | <meta name="viewport" content="width=device-width, initial-scale=1">
| + | |
| − | <link rel="stylesheet" href="main.css">
| + | |
| − | <style>
| + | |
| − | body,h1,h2,h3,h4,h5 {font-family: "Arial", sans-serif}
| + | |
| − | body {font-size:16px;}
| + | |
| − | .w3-half img{margin-bottom:-6px;margin-top:16px;opacity:0.8;cursor:pointer}
| + | |
| − | .w3-half img:hover{opacity:1}
| + | |
| − | </style>
| + | |
| − | <body>
| + | |
| | | | |
| − | <div id="loader-bg"> | + | <title>Human Practice Overview</title> |
| − | <div id="loader">
| + | <meta charset="UTF-8"> |
| − | <div class="photo-show"> | + | <meta name="viewport" content="width=device-width, initial-scale=1"> |
| − | <img src="https://static.igem.org/mediawiki/2017/f/f4/T--TokyoTech--load_1.png" width="600"> | + | |
| − | <img src="https://static.igem.org/mediawiki/2017/4/43/T--TokyoTech--load_2.png" width="600">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | | | |
| − | <!-- Sidebar/menu -->
| |
| − | <nav class="w3-sidebar w3-red w3-collapse w3-top w3-large w3-padding" style="z-index:3;width:300px;font-weight:bold;" id="mySidebar"><br>
| |
| − | <a href="javascript:void(0)" onclick="w3_close()" class="w3-button w3-hide-large w3-display-topleft" style="width:100%;font-size:20px">Close Menu</a>
| |
| − | <div class="w3-container" style="margin-top: 20px;margin-bottom: 25px" id="wrap">
| |
| − | <img src="https://static.igem.org/mediawiki/2018/a/ad/T--Tokyo_Tech--logo_0629.png" style="width: 100%">
| |
| − | </div>
| |
| | | | |
| − | <div style="padding-top: 15px; padding-left: 25px; id="wrap">
| + | <script type="text/javascript" src="https://2018.igem.org/Template:Tokyo_Tech/bootstrap/js/bootstrap.js?action=raw&ctype=text/javascript"></script> |
| | + | <script type="text/javascript" src="https://2018.igem.org/Template:Tokyo_Tech/bootstrap/js/bootstrap.bundle.js?action=raw&ctype=text/javascript"></script> |
| | + | <script type="text/javascript" src="https://2018.igem.org/Template:Tokyo_Tech/js/popper.js?action=raw&ctype=text/javascript"></script> |
| | + | <script type="text/javascript" src="https://2018.igem.org/Template:Tokyo_Tech/bootstrap/jquery/jquery.js?action=raw&ctype=text/javascript"></script> |
| | | | |
| − | <div id="contents" style="z-index: 10">
| + | <style> |
| | + | #home_logo, #sideMenu { display:none; } |
| | + | #sideMenu, #top_title, .patrollink {display:none;} |
| | + | #content { margin-left:0px; margin-top: -15px; padding:0px; width:100%;} |
| | + | body {background-color:white; } |
| | + | #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; } |
| | | | |
| − | <ul class="click_menu_checkbox vertm_down">
| + | .judges-will-not-evaluate { border: 4px solid #e4dede; padding: 2% !important; width: 92%!important;} |
| − | <li style="margin-bottom: 10px; border: 5px double #fff; border-radius: 10px;">
| + | |
| − | <input type="checkbox" id="vmcb-a" />
| + | |
| − | <label for="vmcb-a"><a href="https://2018.igem.org/Team:Tokyo_Tech">Home</a></label>
| + | |
| − | </li>
| + | |
| − | <li style="margin-bottom: 10px; border: 5px double #fff; border-radius: 10px">
| + | |
| − | <input type="checkbox" id="vmcb-b" />
| + | |
| − | <label for="vmcb-b"><a href="https://2018.igem.org/Team:Tokyo_Tech/Medal">Achievements</a></label>
| + | |
| − | </li>
| + | |
| − | <li style="margin-bottom: 10px; border: 5px double #fff; border-radius: 10px">
| + | |
| − | <input type="checkbox" id="vmcb-c" />
| + | |
| − | <label for="vmcb-c"><a>Project</a></label>
| + | |
| − | <ul>
| + | |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Description" class="w3-bar-item w3-button w3-hover-white">Description</a></li>
| + | |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Project/Basic_Parts" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Basic Parts</a></li>
| + | |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Project/Composite_Parts" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Composite Parts</a></li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| | | | |
| − | <li style="margin-bottom: 10px; border: 5px double #fff; border-radius: 10px">
| + | <!-- |
| − | <input type="checkbox" id="vmcb-d" />
| + | /* Fix top panel Bootstrap issues. Patch by iGEM Stockholm 2015. Include this stylesheet in your header, after Bootstrap, to restore the original style to the top menu.*/ |
| − | <label for="vmcb-d"><a>Experiment</a></label>
| + | |
| − | </li>
| + | top_menu_14, |
| | + | #top_menu_under, |
| | + | #top_menu_inside { |
| | + | box-sizing: initial; |
| | + | } |
| | + | |
| | + | top_menu_inside { |
| | + | margin-left: 20px; |
| | + | } |
| | + | |
| | + | bars_box, |
| | + | #menu_BarsBox, |
| | + | h5 { |
| | + | -webkit-box-sizing: initial; |
| | + | box-sizing: initial; |
| | + | line-height: normal; |
| | + | } |
| | + | |
| | + | menu_BarsBox > h5 { |
| | + | color: rgb(0, 0, 0); |
| | + | font-family: arial, sans-serif; |
| | + | font-size: 12px; |
| | + | line-height: normal; |
| | + | margin-bottom: 0px; |
| | + | margin-top: 0px; |
| | + | font-weight: bold; |
| | + | } |
| | + | |
| | + | .accordion-content li { |
| | + | -height: 18px; |
| | + | -webkit-box-sizing: initial; |
| | + | box-sizing: initial; |
| | + | } |
| | + | |
| | + | .accordion-header, |
| | + | .active-header, |
| | + | .accordion-header_noaction { |
| | + | -webkit-box-sizing: initial; |
| | + | box-sizing: initial; |
| | + | color: rgb(101, 100, 89); |
| | + | font-family: Arial, Gadget, sans-serif; |
| | + | font-size: 13.92px; |
| | + | font-weight: bold; |
| | + | line-height: normal; |
| | + | margin: 0; |
| | + | } |
| | | | |
| | | | |
| − | <li style="margin-bottom: 10px; text-align: center; border: 5px double #fff; border-radius: 10px">
| + | #sideMenu, #top_title { |
| − | <input type="checkbox" id="vmcb-e" />
| + | display:none; |
| − | <label for="vmcb-e"><a href="https://2018.igem.org/Team:Tokyo_Tech/Model">Modelling</a></label>
| + | } |
| − | </li>
| + | |
| | | | |
| − | <li style="margin-bottom: 10px; text-align: center; border: 5px double #fff; border-radius: 10px">
| |
| − | <input type="checkbox" id="vmcb-f" />
| |
| − | <label for="vmcb-f"><a>Human Practices</a></label>
| |
| − | <ul>
| |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/HP" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Overview</a></li>
| |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/HP/Silver" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Silver</a></li>
| |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/HP/Gold_Integrated" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Gold (Integrated)</a></li>
| |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Demonstrate" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Demonstrate</a></li>
| |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Collaborations" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Collaborations</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| | | | |
| − | <li style="margin-bottom: 10px; text-align: center; border: 5px double #fff; border-radius: 10px">
| + | #sideMenu { |
| − | <input type="checkbox" id="vmcb-g" />
| + | width: 200px; |
| − | <label for="vmcb-g"><a>About us</a></label>
| + | position: fixed; |
| − | <ul>
| + | } |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Team" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Team</a></li>
| + | |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Attributions" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Attributions</a></li>
| + | |
| − | <li style="text-align: center;"><a href="https://2018.igem.org/Team:Tokyo_Tech/Sponsors" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Sponsors</a></li>
| + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| | | | |
| − | </div>
| + | #content { |
| − | </nav>
| + | width: 100% !important; |
| | + | padding:0px; |
| | + | margin-top:-7px; |
| | + | margin: auto; |
| | + | } |
| | + | |
| | + | .expand_Submenus { height:30px |!important;} |
| | | | |
| − | <!-- Top menu on small screens -->
| + | .menuDisplay.MainPage_menu.main.expand_Submenu { height: 30px |!important;} |
| − | <header class="w3-container w3-top w3-hide-large w3-red w3-xlarge w3-padding">
| + | { |
| − | <a href="javascript:void(0)" class="w3-button w3-red w3-margin-right" onclick="w3_open()"><img src="http://www.adultb2b.biz/wp-content/themes/adultb2b%20-%20new/assets/images/menuBtn.png" style="width: 30px"></a>
| + | width: 170 |!important; |
| − | <span style="padding-top: 5px">iGEM Tokyo Tech</span>
| + | } |
| − | </header>
| + | |
| | + | #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; } |
| | + | --> |
| | | | |
| − | <!-- Overlay effect when opening sidebar on small screens -->
| + | </style> |
| − | <div class="w3-overlay w3-hide-large" onclick="w3_close()" style="cursor:pointer" title="close side menu" id="myOverlay"></div>
| + | |
| | | | |
| − | <!-- PAGE CONTENT! -->
| |
| − | <div class="w3-main" style="margin-left:340px;margin-right:40px">
| |
| | | | |
| − | <div class="w3-container" id="contact" style="margin-top:30px"><!-- ページタイトル -->
| + | </head> |
| − | <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px" align="center">Project Description</h1>
| + | |
| − | </div>
| + | |
| | | | |
| − | <hr> | + | </html> |
| | + | {{Tokyo_Tech/Bootstrap}} |
| | + | <html> |
| | | | |
| − | <div class="w3-container" id="contact" style="margin-top:30px"><!-- ページタイトル --> | + | |
| − | <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px" align="center">Dengue Virus Detection System with multiple colors</h1>
| + | |
| − | </div>
| + | |
| | | | |
| − | <hr> | + | <h1 class="cp_h1title">Attributions</h1> |
| | | | |
| − | <div class="w3-container" id="mecha" style="margin-top:20px"> | + | <br> |
| − | <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px"><b>Mechanism</b></h1>
| + | <p> |
| − | <hr style="width:50px;border:5px solid red" class="w3-round">
| + | Dengue virus, which is in the flavivirus family, is a worldwide spread virus and has huge impact on society, however, not many developing countries are recognizing its danger. |
| | + | </p> |
| | + | <p> |
| | + | Dengue virus is unique in terms of its four different serotypes. Multiple infection can easily cause severe dengue, appearing hemorrhage and organ damage. It is important to grasp which serotype the patient is infected, however, there is not enough data about each serotype in a year. |
| | + | </p> |
| | + | <p> |
| | + | To tackle the situation, we took the following two approaches. |
| | + | </p><ol> |
| | | | |
| − | <center>
| + | <li>We succeeded in the development of the serotype prediction system using stochastic process analysis. This system can predict the patient's serotype by simulating the past data.</li></ol> |
| − | <div style="padding: 10px; margin-bottom: 3px; border: 1px dotted #333333;width: 90%; border-radius: 10px">
| + | |
| − | <h4 style="text-align: center">Virus detection system with multi-colors</h4>
| + | |
| − | <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px">
| + | |
| − | For 2018 project, we try to develop mosaic-envelope virus that can detect the presence of antibodies in serum that correspond to specific type of virus. This mosaic-envelope virus can detect all the serotypes of dengue viruses and can contribute to versatile dengue virus detection. The experimental procedures are categorized as the following three steps.
| + | |
| − | </p>
| + | |
| − | <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px">
| + | |
| − | STEP1: We construct integrated plasmid that codes essential viral proteins (both structural and non-structural) and green fluorescence protein. (Note: If the size is too large, they will be separated.) The envelope (E) genes are derived from 2 or more dengue serotypes so that envelope proteins can be mixed to detect multiple types of antibodies.
| + | |
| − | </p>
| + | |
| − | <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px">
| + | |
| − | STEP2: We transduce the plasmid into suitable eukaryotic cells and culture the cells which are successfully transduced. (Drug selection and a series of passage may be needed.) Most of the transduced cells are expected to produce mosaic-envelope virus.
| + | |
| − | </p>
| + | |
| − | <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px">
| + | |
| − | <p style="font-size:16px;font-size: 16px; text-indent:1em;padding-top: 15px">
| + | |
| − | STEP3: We culture the target mammalian cells that are for checking the infection by mosaic-envelope virus. After mixing the cells and the virus, we measure the fluorescence intensity to check whether the mosaic-envelope virus infected and GFPs are produced or not. As control experiment, we add antibodies that correspond to one of the dengue serotypes to check whether the function of the virus is inhibited or not.
| + | |
| | | | |
| − | </p>
| + | <p> |
| − | </p>
| + | |
| − | <div class="w3-xxxlarge" style="padding-bottom: 3px;text-align: center">
| + | </p><ol> |
| − | <figure>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/4/48/T--Tokyo_Tech--virus_detec_flow_0629.png" style="max-width:80%">
| + | |
| − | <figcaption style="font-size: 16px">Fig. 1 Basic Flow of dengue virus detection system</figcaption>
| + | |
| − | </figure>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | | | |
| − | </center>
| + | <li>We also developed the simple and fast testing kit that can detect serotype with fluorescence, so that we can check the patient easily and get enough data to estimate the patients' serotypes more accurate.</li></ol> |
| | | | |
| − | </div>
| + | <p> |
| − | <!-- End page content -->
| + | In the future, this system can contribute to other flavivirus detection system. |
| − | </div> | + | </p> |
| | | | |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| | | | |
| − | <div class="w3-container" id="contact" style="margin-top:20px">
| + | </div> |
| − | <p id="pageTop" style="text-align:right"><a href="#wrap"><img src="https://static.igem.org/mediawiki/2017/0/0d/T--TokyoTech--page_top_2.png" style="width:200px"></a></p>
| + | |
| − | </div> | + | |
| | | | |
| − | <!-- W3.CSS Container -->
| + | </body> |
| − | <div class="w3-light-grey w3-container w3-padding-32" style="margin-top:75px;padding-right:58px"><p class="w3-right"><a href="http://96haji.me/" title="W3.CSS" target="_blank" class="w3-hover-opacity">Hajime Fujita with W3.CSS: All Rights Reserved</a></p></div>
| + | |
| | | | |
| − | <script src="http://ajax.googleapis.com/ajax/libs/jquery/1.11.1/jquery.min.js"></script>
| + | </html> |
| − | <script>
| + | |
| − | // Script to open and close sidebar
| + | |
| − | function w3_open() {
| + | |
| − | document.getElementById("mySidebar").style.display = "block";
| + | |
| − | document.getElementById("myOverlay").style.display = "block";
| + | |
| − | }
| + | |
| − |
| + | |
| − | function w3_close() {
| + | |
| − | document.getElementById("mySidebar").style.display = "none";
| + | |
| − | document.getElementById("myOverlay").style.display = "none";
| + | |
| − | }
| + | |
| − | </script>
| + | |
| − | <script>
| + | |
| − | // Modal Image Gallery
| + | |
| − | function onClick(element) {
| + | |
| − | document.getElementById("img01").src = element.src; | + | |
| − | document.getElementById("modal01").style.display = "block";
| + | |
| − | var captionText = document.getElementById("caption");
| + | |
| − | captionText.innerHTML = element.alt;
| + | |
| − | }
| + | |
| − | $(function() {
| + | |
| − | var h = $(window).height();
| + | |
| − |
| + | |
| − | $('#wrap').css('display','none');
| + | |
| − | $('#loader-bg ,#loader').height(h).css('display','block');
| + | |
| − | });
| + | |
| − |
| + | |
| − | $(window).load(function () { //全ての読み込みが完了したら実行
| + | |
| − | $('#loader-bg').delay(900).fadeOut(800);
| + | |
| − | $('#loader').delay(600).fadeOut(300);
| + | |
| − | $('#wrap').css('display', 'block');
| + | |
| − | });
| + | |
| − | </script>
| + | |
| − | <script>
| + | |
| − | //■page topボタン
| + | |
| − | $(function(){
| + | |
| − | var topBtn=$('#pageTop');
| + | |
| − | topBtn.hide();
| + | |
| − |
| + | |
| − | //◇ボタンの表示設定
| + | |
| − | $(window).scroll(function(){
| + | |
| − | if($(this).scrollTop()>80){
| + | |
| − | //---- 画面を80pxスクロールしたら、ボタンを表示する
| + | |
| − | topBtn.fadeIn();
| + | |
| − | }else{
| + | |
| − | //---- 画面が80pxより上なら、ボタンを表示しない
| + | |
| − | topBtn.fadeOut();
| + | |
| − | }
| + | |
| − | });
| + | |
| − |
| + | |
| − | // ◇ボタンをクリックしたら、スクロールして上に戻る
| + | |
| − | topBtn.click(function(){
| + | |
| − | $('body,html').animate({
| + | |
| − | scrollTop: 0},500);
| + | |
| − | return false;
| + | |
| − | });
| + | |
| − | | + | |
| − | });
| + | |
| − | </script>
| + | |
| − | | + | |
| − | </body>
| + | |
| − | </html> | + | |
