Difference between revisions of "Team:iTesla-SoundBio/InterLab"

(Prototype team page)
 
Line 1: Line 1:
 
{{iTesla-SoundBio}}
 
{{iTesla-SoundBio}}
 +
 
<html>
 
<html>
  
 +
<style>
 +
p {
 +
    font-size: 20px;
 +
    letter-spacing: .18em;
 +
    padding-left: 100px;
 +
    padding-right: 100px;
 +
}
 +
img {
 +
    display: block;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    width: 50%;
 +
}
 +
table, td, th {   
 +
    border: 1px solid #ddd;
 +
    text-align: left;
 +
}
  
−
<div class="column full_size judges-will-not-evaluate">
+
table {
−
<h3>★  ALERT! </h3>
+
    border-collapse: collapse;
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
+
    width: 50%;
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
}
 +
 
 +
th, td {
 +
    padding: 5px;
 +
}
 +
 
 +
.headers {
 +
color: white;
 +
padding-top: 50px;
 +
height: auto;
 +
}
 +
.middles {
 +
color: white;
 +
padding-top: 10px;
 +
padding-bottomL 10px;
 +
text-align: center;
 +
height: auto;
 +
}
 +
.horizontalline {
 +
    border: 2px solid black;
 +
    width: 50%;
 +
    height: 1 px;
 +
    margin: auto;
 +
    margin-top: 50px;
 +
    margin-bottom: 10px;
 +
}
 +
</style>
 +
 
 +
 
 +
<body>
 +
 
 +
<div class="headers"><p><b><u>Summary</u>:</b> For the 2018 InterLab study, high school students at iTesla-SoundBio prepared various colonies and dilutions to test for trends absorbance, fluorescence, and concentration. <br> Massive thanks to the Washington iGEM InterLab team for guiding us through the protocol and lending us their plate readers. </P>
 +
<p>Below, is the timeline that we followed in completing the InterLab protocol.</p></div>
 +
 
 +
 
 +
<img src="https://static.igem.org/mediawiki/2018/5/55/T--iTesla-SoundBio--interlabtimeline.jpg" alt= "ludoxprotocol" style="width:80%" align= "middle">
 +
 
 +
<div class="horizontalline">
 
</div>
 
</div>
  
 +
<div class="middles"><h1>Part One- LUDOX Calibration Protocol</h1></div>
  
−
<div class="clear"></div>
+
<div class="middles"><h2>96 Well Plate Table</h2></div>
  
 +
<table align="center">
 +
  <tr>
 +
    <th>  </th>
 +
    <th>1</th>
 +
    <th>2</th>
 +
    <th>3</th>
 +
    <th>4</th>
 +
    <th>5</th>
 +
    <th>6</th>
 +
    <th>7</th>
 +
    <th>8</th>
 +
    <th>9</th>
 +
    <th>10</th>
 +
    <th>11</th>
 +
    <th>12</th>
 +
  </tr>
 +
  <tr>
 +
    <td>A</td>
 +
    <td>LUDOX </td>
 +
    <td>ddH2O</td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
  
−
<div class="column full_size">
+
  </tr>
−
<h1>InterLab</h1>
+
  <tr>
−
<h3>Bronze Medal Criterion #4</h3>
+
    <td>B</td>
−
<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
+
    <td>LUDOX </td>
−
<br><br>
+
    <td>ddH2O</td>
−
For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
+
    <td>  </td>
 +
    <td>  </td>
 +
    <td> </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
  </tr>
 +
  <tr>
 +
    <td>C</td>
 +
    <td>LUDOX </td>
 +
    <td>ddH2O</td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
  </tr>
 +
  <tr>
 +
    <td>D</td>
 +
    <td>LUDOX </td>
 +
    <td>ddH2O</td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
  </tr>
 +
  <tr>
 +
    <td>E  </td>
 +
    <td> </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
  </tr>
 +
  <tr>
 +
      <td>F  </td>
 +
    <td> </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
  </tr>
 +
  <tr>
 +
    <td>G  </td>
 +
    <td> </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
  </tr>
 +
  <tr>
 +
    <td>H  </td>
 +
    <td> </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
    <td>  </td>
 +
  </tr>
 +
</table>
 +
<div class="middles">
 +
<p>Here are the results for the Absorbance 600 of the LUDOX protcol</p>
 +
<h2> OD600 Reference Point Data </h2>
 +
</div>
  
−
</p>
+
<img src="https://static.igem.org/mediawiki/2018/8/8b/T--iTesla-SoundBio--LUDOX_absorbance_readings.jpeg.JPG" alt= "ludoxprotocol">
 +
 
 +
 
 +
<div class="horizontalline">
 
</div>
 
</div>
  
  
 +
<div class="middles">
 +
<h1> Part Two- Microsphere Protocol</h1>
 +
</div>
 +
<img src="https://static.igem.org/mediawiki/2018/f/f6/T--iTesla-SoundBio--microspherestandardcurvelinear.jpg" alt= "microspherecurve", style="width:40%;">
  
 +
<img src="https://static.igem.org/mediawiki/2018/6/64/T--iTesla-SoundBio--microspherestandardcurvelog.jpg" alt= "microsphere log curve", style="width:40%;">
  
 +
 +
<div class="horizontalline">
 +
</div>
 +
 +
 +
 +
<div class="middles"><h1> Part Three- Fluorescein Protocol</h1><div>
 +
 +
<img src="https://static.igem.org/mediawiki/2018/4/44/T--iTesla-SoundBio--fluoresceinlinear.jpg" alt= "fluoresciencurve", style="width:40%;">
 +
 +
<img src="https://static.igem.org/mediawiki/2018/5/51/T--iTesla-SoundBio--fluorescein.jpg" alt= "microsphere log curve", style="width:40%;">
 +
 +
 +
 +
<div class="horizontalline">
 +
</div>
 +
 +
 +
<div class="middle">
 +
<h1>Bronze Medal Criterion #4</h1>
 +
</div>
 +
 +
<div class="middles">
 +
<p>
 +
<b>Standard Tracks:</b>
 +
<br>
 +
Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
 +
<br>
 +
For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab"> InterLab study </a>, all work must be shown on this page.
 +
</p>
 +
</div>
 +
<img src="https://static.igem.org/mediawiki/2018/f/fe/T--iTesla-SoundBio--interlabjoy.jpg" alt= "interlab joy", style="width:80%;">
  
  
  
 +
<script>
 +
    $("#title").text("InterLab")
 +
</script>
 +
</body>
 
</html>
 
</html>

Revision as of 23:47, 23 September 2018

Safety Protocols
InterLab
Team Members Attributions
Human Practices Collaborations
Project Description Design Results Biobrick Parts Lab Notebook
iT-SB 2018 iT-SB 2017

Summary: For the 2018 InterLab study, high school students at iTesla-SoundBio prepared various colonies and dilutions to test for trends absorbance, fluorescence, and concentration.
Massive thanks to the Washington iGEM InterLab team for guiding us through the protocol and lending us their plate readers.

Below, is the timeline that we followed in completing the InterLab protocol.

ludoxprotocol

Part One- LUDOX Calibration Protocol

96 Well Plate Table

1 2 3 4 5 6 7 8 9 10 11 12
A LUDOX ddH2O
B LUDOX ddH2O
C LUDOX ddH2O
D LUDOX ddH2O
E
F
G
H

Here are the results for the Absorbance 600 of the LUDOX protcol

OD600 Reference Point Data

ludoxprotocol

Part Two- Microsphere Protocol

microspherecurve microsphere log curve

Part Three- Fluorescein Protocol

fluoresciencurve microsphere log curve

Bronze Medal Criterion #4

Standard Tracks:
Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
For teams participating in the InterLab study , all work must be shown on this page.

interlab joy