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Revision as of 02:56, 24 September 2018
Welcome to iGEM 2018!
Your team has been approved and you are ready to start the iGEM season!
Abstract
Cell lines HaCat and L-929 were used to evaluate a tissue regeneration system incorporating a collagen-based scaffold and encapsulated recombinant growth factors. A collagenous synthetic gene comprising collagen type V heparin binding domain (HepV) was expressed in a bacterial system, constituting the main component of the scaffold. For collagen molecule consists of repetitive G-X-Y triplets, being Y frequently hydroxyproline (Hyp), a method of intracellular accumulation of Hyp is used to co-translationally incorporate this non-canonical amino acid. A chitosan crosslinking procedure is carried out on the expressed collagen to prevent contraction and to finally freeze-dry. Further, a mixture of heparin and tenascin C fibronectin type III domain V (TNCIII5) was added to the scaffold, for the heparin-tenascin C binding has a high affinity with a large number of growth factors. Recombinant human leptin β (LepB) is used as a growth factor and we hypothesize that it may be encapsulated to achieve a controlled drug delivery. Due to its suitability, chitosan nanoencapsulation was employed. To evaluate the efficacy of the system, MTT proliferation assay was performed in 96-well plates. Given the cell lines assessed during this project, this tissue regeneration system proves its ability to be used in the treatment of second degree burns. As a widely new biotechnology procedure for skin regeneration the creation of a new, low cost method, for the silencing of miRNA´s involved in the skin regeneration was created and tested. The project was also tested using a skin chip as a practical and small cell proliferation hardware.
Before you start
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Uploading pictures and files
You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2018 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples: