Difference between revisions of "Team:Munich/thisisatest.html"
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<div class="event-info"> | <div class="event-info"> | ||
| + | |||
| + | <h3>5/7</h3> | ||
| + | <h4>Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</h4> | ||
| + | <table class="table table-borderless"> | ||
| + | <tr> | ||
| + | |||
| + | <td>Participants:</td> | ||
| + | <td>Dominic</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Protocol:</td> | ||
| + | <td>epo. Trafo</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Notes:</td> | ||
| + | <td>pkD3 contains resistance cassettes with FRT-sites for pRED engineering <br> | ||
| + | incubate pRED at 30°C because of temperature sensitive promoter | ||
| + | <br> | ||
| + | pNPTS138-R6KT is for knock-ins via RecA Recombineering | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Results:</td> | ||
| + | <td>no colonies</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
<h3>5/18</h3> | <h3>5/18</h3> | ||
Revision as of 14:24, 25 September 2018
5/7
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | pkD3 contains resistance cassettes with FRT-sites for pRED engineering incubate pRED at 30°C because of temperature sensitive promoter
|
| Results: | no colonies |
5/18
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter |
| Results: | no colonies |
5/24
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter, pKD3 contains resistance cassette flanked by FRT sites |
| Results: | no colonies |
5/25
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | chem. Trafo |
| Notes: | pKD3 contains resistance cassette flanked by FRT sites |
| Results: | no colonies |
5/26
DNA preparation for pRED/ET Engineering
| Participants: | Dominic |
| Protocol: | mini prep |
| Notes: | because pRED/ET could not be transformed, we got readily transformed cells from PD Dr. Jürgen Lassak from the LMU |
| Results: | pRED/ET: 37,5 ng/ul
pNPTS138-R6KT: 60ng/ul |