Difference between revisions of "Team:Munich/chiversions.html"
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<div class="event-info"> | <div class="event-info"> | ||
| + | |||
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| + | <h3>5/28</h3> | ||
| + | <h4>PCR amplify linear mtq2</h4> | ||
| + | <table class="table table-borderless"> | ||
| + | <tr> | ||
| + | |||
| + | <td>Participants:</td> | ||
| + | <td>Dominic</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Protocol:</td> | ||
| + | <td>PCR</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Notes:</td> | ||
| + | <td>VR & VF2; AT: 66° ET: 50s | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Results:</td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | |||
| + | <h3>5/29</h3> | ||
| + | <h4>Gel purification of linear mtq2</h4> | ||
| + | <table class="table table-borderless"> | ||
| + | <tr> | ||
| + | |||
| + | <td>Participants:</td> | ||
| + | <td>Dominic</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Protocol:</td> | ||
| + | <td>Gel purification</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Notes:</td> | ||
| + | <td>PIC | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Results:</td> | ||
| + | <td>worked</td> | ||
| + | </tr> | ||
| + | </table> | ||
<h3>6/1</h3> | <h3>6/1</h3> | ||
Revision as of 14:44, 25 September 2018
5/28
PCR amplify linear mtq2
| Participants: | Dominic |
| Protocol: | PCR |
| Notes: | VR & VF2; AT: 66° ET: 50s |
| Results: |
5/29
Gel purification of linear mtq2
| Participants: | Dominic |
| Protocol: | Gel purification |
| Notes: | PIC |
| Results: | worked |
6/1
Forming oligodimers from single-strand DNA chi6 sequences
| Participants: | Dominic |
| Protocol: | Oligodimerization |
| Notes: | |
| Results: |
6/4
PCR to assemble Chi6(double) and Chi6-Mtq2-Chi6
| Participants: | Dominic |
| Protocol: | PCR |
| Notes: | |
| Results: | no bands on gel |
6/8
Gibson assembly of Chi6(double) and Chi6-Mtq2-Chi6
| Participants: | Eni |
| Protocol: | Gibson assembly |
| Notes: | M(100bp) / / 2x / flank / BBP-lacO / Term-BBS |
| Results: | no bands on gel |
6/11
PCR to assemble Chi6(double) and Chi6-Mtq2-Chi6
| Participants: | Dominic |
| Protocol: | PCR |
| Notes: | |
| Results: | no bands on gel |
6/12
Gelextraction of Fragments to assembly Chi6 Versions
| Participants: | Dominic |
| Protocol: | Gelextraction |
| Notes: | |
| Results: | concentration, GelPIC |
6/12
PCR to amplify Mtq2
| Participants: | Dominic |
| Protocol: | PCR |
| Notes: | |
| Results: | no bands on gel |
6/14
PCR to assemble Chi6(double) and Chi6-Mtq2-Chi6
| Participants: | Dominic |
| Protocol: | PCR |
| Notes: | |
| Results: | lost samples |