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Revision as of 10:34, 26 September 2018
Home
Interlab
Fifth International InterLab Measurement Study
Overview
Interlab procedure was made to achieve bronze #4 requirement. To make this, the IGEM 2018 Interlab Study Protocol was followed. To make calibration and experimental data the following material was used: measurement kit which contains Ludox, silica bead and fluorescein, competent cells from E. coli strain DH5a and 96 well plates, black with a clear flat bottom. The first part of the protocol was to make standard curves for OD measurements, cell standard curves and fluorescein standard curves. Using Ludox solution, it was capable to obtain the following reference point for an OD600.
Transformation
Absorbance
Fluorescence