Difference between revisions of "Team:NUS Singapore-A/Modelling/Model1"
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Modelling was heavily utilised to obtain a better understanding of our systems, as well as, shaping our experimental designs which helped us save unnecessary wastage of resources and time. We constructed models that allowed us to achieve the following: | Modelling was heavily utilised to obtain a better understanding of our systems, as well as, shaping our experimental designs which helped us save unnecessary wastage of resources and time. We constructed models that allowed us to achieve the following: | ||
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Our team started with the intention to produce dyes consisting of the primary colours, red, yellow and blue, that can be easily mixed to create a plethora of colours for the textile industry. However, we have decided to produce namely, Chrysanthemin (red) and Luteolin (yellow) instead. This decision was due in part to our interview with Mr Holger Schlaefke (link to our interview here), Global Marketing Manager of DyStar Pte Ltd, that advised us to produce vibrant colours, and how numerous past iGEM teams have attempted to produce Indigo (a colour similar to blue). | Our team started with the intention to produce dyes consisting of the primary colours, red, yellow and blue, that can be easily mixed to create a plethora of colours for the textile industry. However, we have decided to produce namely, Chrysanthemin (red) and Luteolin (yellow) instead. This decision was due in part to our interview with Mr Holger Schlaefke (link to our interview here), Global Marketing Manager of DyStar Pte Ltd, that advised us to produce vibrant colours, and how numerous past iGEM teams have attempted to produce Indigo (a colour similar to blue). | ||
| − | < | + | <h2>PART 1: THE BIOCHEMICAL PATHWAY </h2> |
| − | Goal of model | + | <i>Goal of model</i> |
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| + | <p> Thus, Wet Lab needs to verify the feasibility of producing these two flavone compounds in E.Coli from the starting substrate, Naringenin, by considering only the biochemical reactions using mathematical modelling in-silico (refer to the biochemical pathway in Fig 1.). In addition to this, we explore how enzyme concentrations affect our system in hopes that it may aid us in our experimental design. </p> | ||
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| + | <img src="img_girl.jpg" width="500" height="600"> | ||
| + | <p style="text-align:center;"> <i> Fig 1. Biochemical Pathway from starting substrate Naringenin </i></p> | ||
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| + | </body> | ||
Revision as of 19:53, 27 September 2018
OVERVIEW
Modelling was heavily utilised to obtain a better understanding of our systems, as well as, shaping our experimental designs which helped us save unnecessary wastage of resources and time. We constructed models that allowed us to achieve the following: 1. Preliminary study of our intended biochemical pathway 2. Optimal genetic circuit design 3. Proof that optogenetics can work in our systems and improve upon existing inducible/repressible light systems 4. Simulation and optimisation of our entire experimental process Our MATLAB scripts can be found here.
Our team started with the intention to produce dyes consisting of the primary colours, red, yellow and blue, that can be easily mixed to create a plethora of colours for the textile industry. However, we have decided to produce namely, Chrysanthemin (red) and Luteolin (yellow) instead. This decision was due in part to our interview with Mr Holger Schlaefke (link to our interview here), Global Marketing Manager of DyStar Pte Ltd, that advised us to produce vibrant colours, and how numerous past iGEM teams have attempted to produce Indigo (a colour similar to blue).
PART 1: THE BIOCHEMICAL PATHWAY
Goal of model
Thus, Wet Lab needs to verify the feasibility of producing these two flavone compounds in E.Coli from the starting substrate, Naringenin, by considering only the biochemical reactions using mathematical modelling in-silico (refer to the biochemical pathway in Fig 1.). In addition to this, we explore how enzyme concentrations affect our system in hopes that it may aid us in our experimental design.
<img src="img_girl.jpg" width="500" height="600">
Fig 1. Biochemical Pathway from starting substrate Naringenin
</body>