Difference between revisions of "Team:Munich/recbiobricks.html"

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<h3>8/31</h3>
 
<h3>8/31</h3>
<h4>Assembling biobrick for RecB</h4>
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<h4>Assembling biobrick for RecB, C, D</h4>
 
<table class="table table-borderless">
 
<table class="table table-borderless">
 
     <tr>
 
     <tr>
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     <tr>
 
     <tr>
 
       <td>Protocol:</td>
 
       <td>Protocol:</td>
       <td>PCR</td>
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       <td>PCR, agarose gel, gel extraction, ligation, chem trafo, miniprep </td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
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  <tr>
 
  <tr>
 
       <td>Results:</td>
 
       <td>Results:</td>
       <td>PIC</td>
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       <td>PIC only one worked, but somehow all were ligated :D</td>
 
     </tr>
 
     </tr>
 
</table>
 
</table>
  
 
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Revision as of 15:31, 28 September 2018


8/31

Assembling biobrick for RecB, C, D

Participants: eni, katja
Protocol: PCR, agarose gel, gel extraction, ligation, chem trafo, miniprep
Notes: RecD_BB_EcoRI_fw & RecD_BB_SpeI_rv (3,5 kb)

RecB_BB_EcoRI_fw & RecB_BB_EcoRi_rv (1,8 kb)
RecC_BB_EcoRI_fw & RecB_BB_EcoRI_rv (3,3 kb)
AT 68° ET 70 s

Results: PIC only one worked, but somehow all were ligated :D