Line 3: | Line 3: | ||
<h3>8/31</h3> | <h3>8/31</h3> | ||
− | <h4>Assembling biobrick for RecB</h4> | + | <h4>Assembling biobrick for RecB, C, D</h4> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
Line 12: | Line 12: | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
− | <td>PCR</td> | + | <td>PCR, agarose gel, gel extraction, ligation, chem trafo, miniprep </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 24: | Line 24: | ||
<tr> | <tr> | ||
<td>Results:</td> | <td>Results:</td> | ||
− | <td>PIC</td> | + | <td>PIC only one worked, but somehow all were ligated :D</td> |
</tr> | </tr> | ||
</table> | </table> | ||
</div> | </div> |
Revision as of 15:31, 28 September 2018
8/31
Assembling biobrick for RecB, C, D
Participants: | eni, katja |
Protocol: | PCR, agarose gel, gel extraction, ligation, chem trafo, miniprep |
Notes: | RecD_BB_EcoRI_fw & RecD_BB_SpeI_rv (3,5 kb) RecB_BB_EcoRI_fw & RecB_BB_EcoRi_rv (1,8 kb) |
Results: | PIC only one worked, but somehow all were ligated :D |