Difference between revisions of "Team:Toulouse-INSA-UPS/Public Engagement"
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<h2 class="heavy">PUBLIC ENGAGEMENT : EXPERT'S ADVICE</h2> | <h2 class="heavy">PUBLIC ENGAGEMENT : EXPERT'S ADVICE</h2> | ||
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| − | <p> | + | <p style="text-indent: 25px;">The Cerberus project was very ambitious, even from start. Somehow, it was not one project but several since we decided to tackle several problematics, even if with one universal solution. This universality came at a cost since grafting organic and inorganic molecules is a very different challenge. For organic ones, we had synthetic biology with us. But for inorganic one, the challenge was to gathered biology and chemistry, the latter being, at the project start, a big unknown field for us. We therefore had to explain our project with international and national experts, get their feedbacks, and integer it in our design. Here are some of the most important one for us. |
| − | + | </p> | |
| − | <h3 class="heavy"> | + | <h3 class="heavy">Han Xiao</h3> |
| + | <p><i>Unnatural amino acids, Rice University (Houston, Texas, United States)</i></p> | ||
<hr/> | <hr/> | ||
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<div class="col-12"> | <div class="col-12"> | ||
| − | <img src=" | + | <img src="https://static.igem.org/mediawiki/2018/6/6c/T--Toulouse-INSA-UPS--Team--Han.jpg" style="width:170px; height:201px" class="img-thumbnail mr-2 ml-0 mt-0 mb-0 float-left" alt="Expert 1 Picture"/> |
| − | <p> | + | <p style="text-indent: 25px;"> |
| − | + | Han Xiao is an Assistant Professor of Chemistry, and Biosciences at Rice University (Houston, TX). After graduating from USTC in 2010, Han joined the Ph.D. program at the Scripps Research Institute (TSRI). His thesis work with Prof. Peter G. Schultz focused on expanding the technique of genetically incorporating unnatural amino acids in both prokaryotic and eukaryotic organisms and applying this technique for better cancer therapeutics. In 2015, Han joined the laboratory of Prof. Carolyn R. Bertozzi as a Good Ventures Postdoctoral Fellow of the Life Science Research Foundation at the Stanford University. In his postdoctoral work, he is engaged in the development of novel cancer immunotherapy targeting the cell-surface glycans axis of immune modulation. | |
| − | <p> | + | </p> |
| − | + | <p style="text-indent: 25px;"> | |
| − | <p> | + | Delving into the world of unnatural amino acids was a first for our iGEM team, so naturally we had a lot of questions about how to proceed. Han Xiao gracefully answered these questions and provided us with much needed insight into the various problems we could face during our project. |
| + | </p> | ||
| + | <p style="text-indent: 25px;"> | ||
| + | With his help, we were able to best determine the critical elements of our experiments. We identified the steps which would require the most care and precision, as well as which transformation strategies to pursue. | ||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| − | + | <h3 class="heavy">Kathrin Lang</h3> | |
| + | <p><i>Organic chemistry, Technical University of Munich, Institute for Advance Study (Munich, Germany)</i></p> | ||
| + | <hr/> | ||
| + | <div class="container-fluid p-0 m-0"> | ||
| + | <div class="row"> | ||
| + | <div class="col-12"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/7/74/T--Toulouse-INSA-UPS--Team--Kathrin.jpg" style="width:170px; height:201px" class="img-thumbnail mr-2 ml-0 mt-0 mb-0 float-left" alt="Expert 2 Picture" /> | ||
| + | <p style="text-indent: 25px;"> | ||
| + | Professor Lang conducts research in the interdisciplinary area of chemical biology, applying concepts from organic chemistry to develop new tools to study fundamental biological questions. Her research focuses on the targeted chemical synthesis of new artificial biomolecules (amino acids, proteins, nucleotides, oligonucleotides) tailored to investigate and manipulate complex cellular processes in in vitro and in vivo biological systems. | ||
| + | During the European meet-up hosted by the iGEM Munich team, she presented her works on UAA and their use in enzymology. We then had the opportunity to meet Pr. Lang and receive her advices concerning our project, after showing her our poster. | ||
| + | </p> | ||
| + | <p style="text-indent: 25px;"> | ||
| + | This was a great boosting event for us. We liked her commenting “There’s no reason for it not to work”! Her comments on our general design also helped us progress further in our project. | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <h3 class="heavy"> | + | <h3 class="heavy">Philippe Serp</h3> |
| + | <p><i>Inorganic Chemistry, ENSIACET (Toulouse, France)</i></p> | ||
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| − | <img src=" | + | <img src="https://static.igem.org/mediawiki/2018/2/23/T--Toulouse-INSA-UPS--Team--Philippe.jpg" style="width:170px; height:201px" class="img-thumbnail mr-2 ml-0 mt-0 mb-0 float-left" alt="Expert 3 Picture" /> |
| − | <p> | + | <p style="text-indent: 25px;"> |
| + | Philippe Serp is a researcher at the “Laboratoire de Chimie de Coordination» and professor at ENSIACET (National School of Engineering in Chemical and Technological Arts). | ||
| + | </p> | ||
| + | <p style="text-indent: 25px;"> | ||
| + | We met Mr. Serp to get advice on an idea we had about using graphene to functionalize cellulose. Thanks to his advices, we have been able to modify our project to optimize our chances of success. Initially starting with the use of oxidized graphene in our project, we have, on his advice, changed to non-oxidized graphene. He also helped us to optimize our functionalization protocols with the assistance of Jerome Volkman, an organic chemist. They were kind enough to | ||
| + | allow us manipulating in his laboratory to functionalize graphene. Indeed, we succeeded in adding DBCO chemical groupement to graphene with their help. It is just a pity we did not have enough time to test graphene on cellulose and we deeply apologize to Philippe and his team for that. | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | + | ||
| − | <h3 class="heavy"> | + | <h3 class="heavy">Jeremy Esque</h3> |
| + | <p><i>Modelling, LISBP (Toulouse, France)</i></p> | ||
<hr/> | <hr/> | ||
<div class="container-fluid p-0 m-0"> | <div class="container-fluid p-0 m-0"> | ||
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<div class="col-12"> | <div class="col-12"> | ||
| − | <img src=" | + | <img src="https://static.igem.org/mediawiki/2018/6/68/T--Toulouse-INSA-UPS--Team--Jeremy.jpg" style="width:170px; height:201px" class="img-thumbnail mr-2 ml-0 mt-0 mb-0 float-left" alt="Expert 4 Picture" /> |
| − | <p> | + | <p style="text-indent: 25px;"> |
| + | Given the central role of the Cerberus linker in our project, it was crucial to modelize its biochemical structure. We soon discover this was not a trivial challenge since parts of the molecule was of unknown structure (AzF, the junctions between CBM3a and the other heads for instance). Fortunately, we had the assistance of Jeremy Esque, INRA Research Engineer at the Toulouse LISBP. Jeremy is a specialist in structural bioinformatics. Its competences in structural dynamics of biomolecules have been an invaluable asset to our project, especially for the in silico assessment of Cerberus stability. | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <h3 class="heavy"> | + | <h3 class="heavy">Christine Lafforgue</h3> |
| + | <p><i>Fermentation processes, LISBP (Toulouse, France)</i></p> | ||
<hr/> | <hr/> | ||
<div class="container-fluid p-0 m-0"> | <div class="container-fluid p-0 m-0"> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-12"> | <div class="col-12"> | ||
| − | <img src=" | + | <img src="https://static.igem.org/mediawiki/2018/8/8d/T--Toulouse-INSA-UPS--Team--Christine.jpg" style="width:170px; height:201px" class="img-thumbnail mr-2 ml-0 mt-0 mb-0 float-left" alt="Expert 5 Picture"/> |
| − | <p> | + | <p style="text-indent: 25px;"> |
| + | Our first idea was to produce functionalized cellulose in vivo. So we aimed to produce Cerberus and its associated function by the yeast Pichia pastoris in bioreactor. We had long discussion about this with Christine Lafforgue. She is a specialist in fermentation process and her insight was invaluable to our project since it avoided us lot of difficulties. Indeed, it came out from our discussion that cellulose production in bioreactor was a very risky and required tangential flow filtration to maintain a productive system. We therefore reshaped our project and put most of our strain in a E. coli driven production with subsequent purification. In vivo production is still a possibility for Cerberus, but it was no more a priority and the results prove her right. | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 21:12, 28 September 2018
EDUCATION & PUBLIC ENGAGEMENT
INTRODUCTION GENERALE
SCIENCE EDUCATION
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SCIENCE EDUCATION : PRESS
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SCIENCE EDUCATION : SCHOOL
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PRIMARY SCHOOL
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"SCIENCE AND TECHNOLOGY OF LABORATORY" HIGHSCHOOL
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INTERNATIONAL HIGHSCHOOL
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UNIVERSITARY INSTITUT OF TECHNOLOGY
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SCIENCE EDUCATION : EVENTS
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EUROSCIENCE OPEN FORUM
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"TOUR DES SCIENCES"
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"GRIMOIRE"
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SCIENCE EDUCATION : MINISTERE DE L'EDUCATION NATIONALE
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TITLE
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TITLE
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TITLE
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SCIENCE EDUCATION : MICROBIOWORLD
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THE GAME
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CREATION OF MICROBIOWORLD V2 : IMPROVEMENTS OF THE GAME
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MICROBIOWORLD AND SCIENCE EDUCATION
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RULES & CARDS
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Preparation
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Turn
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Bacteria Cards
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Media Cards
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Plasmid Cards
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Event Cards
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
Nulla facilisi. Maecenas condimentum nisi sem, sit amet facilisis sapien ultricies sit amet. Mauris ut sollicitudin orci, nec vulputate nisl. Nulla lacinia odio quis lectus tristique, in auctor massa blandit. Duis et aliquam metus. Nulla tristique mi velit, a cursus justo fringilla sit amet. Vestibulum id tortor a mi porta convallis sit amet vel justo. Phasellus egestas viverra urna non ultrices. Praesent sit amet enim porta nisl fermentum elementum vitae et diam. Phasellus consectetur tincidunt est. Interdum et malesuada fames ac ante ipsum primis in faucibus.
ACKNOWLEDGMENTS
Nunc nec nunc sit amet mi condimentum pretium. Vivamus molestie cursus tempus. Ut sed justo in ante sodales dignissim non euismod magna. Nullam hendrerit sem eget lacus egestas, et viverra turpis lobortis. Morbi justo sapien, imperdiet in augue ac, accumsan iaculis urna. Sed mollis purus et urna porta lobortis. Proin consequat magna ac massa maximus vulputate. Cras pellentesque volutpat varius. Pellentesque sit amet sapien eu felis viverra imperdiet ut vehicula diam. Fusce tristique ex a tellus facilisis sagittis vitae ac purus. Sed in nunc ut nisi tincidunt porttitor a sit amet sapien.
PUBLIC ENGAGEMENT
Nunc nec nunc sit amet mi condimentum pretium. Vivamus molestie cursus tempus. Ut sed justo in ante sodales dignissim non euismod magna. Nullam hendrerit sem eget lacus egestas, et viverra turpis lobortis. Morbi justo sapien, imperdiet in augue ac, accumsan iaculis urna. Sed mollis purus et urna porta lobortis. Proin consequat magna ac massa maximus vulputate. Cras pellentesque volutpat varius. Pellentesque sit amet sapien eu felis viverra imperdiet ut vehicula diam. Fusce tristique ex a tellus facilisis sagittis vitae ac purus. Sed in nunc ut nisi tincidunt porttitor a sit amet sapien.
PUBLIC ENGAGEMENT : INTERVIEW
A REMPLIR
OUR APPROACH
A REMPLIR
SYNTHESIS OF OUR INTERVIEWS
A REMPLIR
Interview 1083
David Leromain, Innovation Manager, August the 13th 2018
- Presentation of the 1083 Company
- Current operation
- Our innovation
1083 is a trademark of the company Novetex. It was founded in 2007 by a computer manager who wanted to reconvert himself in the sale of ecological clothing. The founder wanted to create his own business. He chose jeans because the universality of this garment (beyond nationalities, religions, ages, etc). Jeans are also linked to the French industry with the Nîmes canvas. 1083 then diversified by offering sneakers, bermudas, skirts and shorts. The question of what was possible to do in France then arose. A first reflection was made on the economy. Many companies in the textile industry use a large number of subcontractors, which entails considerable additional costs. 1083 minimizes the number of intermediaries (manufacturers, brand and reseller) by turning toward a traditional economic model, eco responsible and French manufacturing.
Jeans are complex products that are produced in several stages: cotton harvesting, spinning, dyeing, weaving, finishing, design and finishing. Once manufactured, the jeans are sold and used, then discarded or recycled. Cotton growing is one of the few stages in the production of the product that 1083 is forced to do abroad. Indeed, the French climate is not compatible with this type of culture.
The cotton spinning makes it possible to obtain a ribbon which will be transformed into wire. The longer the fibers, the more resistant the wire will be. Traditionally, jeans are made using blue yarn, dyed with indigo (the natural color of cotton being white). Weaving is the stage that makes it possible to produce fabric from yarn. When weaving, the thread has to be taut. Engraving allows the raw canvas to stabilize. The fabric will get wet and then heated between two rolls. Wet, the vegetable fibers are compressed which makes the fabric shrink. Once dry and at the exit of the rollers, the fabric is then stretched to make it regain its initial size. Then come the cutting of the fabric, the assembly of the different parts and the mounting of the accessories. The raw material itself will be revalued by recycling. Jeans can be converted into Bermuda shorts or shorts for example. The fabric can also be used as insulation or as fuel.
Although organic cotton reduces the environmental impact due to the absence of pesticides, it is very water-hungry, as is the fixing of indigo. Using bacterial cellulose could reduce this ecological impact. When spinning, the uniform yarn quality has to be maintained, different arrivals of cottons are thus mixed and spun together. Bacterial cellulose has the advantage of being of constant quality because the entire production environment is perfectly controlled.
The dyes that 1083 create problem because the color of the jeans will not wear out. However, it is a characteristic of jeans that is sought by consumers. The molecules used for our project risk, on the contrary, to be fixed only to the superficial part of the wire and be very quickly unhooked during the use of the jeans which would cause the desired discoloration. The textile produced by bacteria without cotton can be interesting provided it remains clean and ecological. On the other hand, we must try to think about the recycling of the material.
Interview Prodhex
August the 20th 2018
- Presentation of the Prodhex Company
- Current operation
- Our innovation
Prodhex is a company founded by René Jambon in 2002 as a first aid specialist. It offers emergency kits, bandages and many types of dressings. They supply mainly French firefighters but also the Samu, the French Red Cross or Pharmacists.
We were able to get an interview with the current director Mr. Jambon who was able to present us the operation of his company and give us his opinion on our project.
Medical products are subject to a great deal of control when producing and sterilizing them. That's why the Prodhex Company takes special care in the production of its dressings, bandages and emergency products. The roll of tissue that will be in contact with the wound may be spun, non-woven, plastic or adhesive. Tissue rolls (60 to 80 m) are placed in an oven. Then adding the glue is done on the fabric. The rolls are then rolled up with adhesive and cut (5 to 10 cm depending on the dressings). Once cut, the dressing will be equipped with a central or complete compress. In the case of strips of 1m * 6m, the compress is continuous and cut every meter. Sterilization is subsequently gamma rays. The most sensitive part is the precise placement of tissue for cutting but also gamma sterilization.
After discussing our protein Cerberus and its fixation on dressings, Mr. Jambon told us that our product was part of the current industry. Our protein would cause less risk for the patient but also for the environment. Indeed the current fixing techniques are chemical and can be for example corrosive. The areas of healing and anti-bacterial are in full swing and need to attach directly to the dressing in a less harmful way for example.
PUBLIC ENGAGEMENT : SURVEY
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OUR APPROACH
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OUR SURVEY
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STATISTICAL ANALYSIS
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COLLABORATION
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PUBLIC ENGAGEMENT : EXPERT'S ADVICE
The Cerberus project was very ambitious, even from start. Somehow, it was not one project but several since we decided to tackle several problematics, even if with one universal solution. This universality came at a cost since grafting organic and inorganic molecules is a very different challenge. For organic ones, we had synthetic biology with us. But for inorganic one, the challenge was to gathered biology and chemistry, the latter being, at the project start, a big unknown field for us. We therefore had to explain our project with international and national experts, get their feedbacks, and integer it in our design. Here are some of the most important one for us.
Han Xiao
Unnatural amino acids, Rice University (Houston, Texas, United States)
Han Xiao is an Assistant Professor of Chemistry, and Biosciences at Rice University (Houston, TX). After graduating from USTC in 2010, Han joined the Ph.D. program at the Scripps Research Institute (TSRI). His thesis work with Prof. Peter G. Schultz focused on expanding the technique of genetically incorporating unnatural amino acids in both prokaryotic and eukaryotic organisms and applying this technique for better cancer therapeutics. In 2015, Han joined the laboratory of Prof. Carolyn R. Bertozzi as a Good Ventures Postdoctoral Fellow of the Life Science Research Foundation at the Stanford University. In his postdoctoral work, he is engaged in the development of novel cancer immunotherapy targeting the cell-surface glycans axis of immune modulation.
Delving into the world of unnatural amino acids was a first for our iGEM team, so naturally we had a lot of questions about how to proceed. Han Xiao gracefully answered these questions and provided us with much needed insight into the various problems we could face during our project.
With his help, we were able to best determine the critical elements of our experiments. We identified the steps which would require the most care and precision, as well as which transformation strategies to pursue.
Kathrin Lang
Organic chemistry, Technical University of Munich, Institute for Advance Study (Munich, Germany)
Professor Lang conducts research in the interdisciplinary area of chemical biology, applying concepts from organic chemistry to develop new tools to study fundamental biological questions. Her research focuses on the targeted chemical synthesis of new artificial biomolecules (amino acids, proteins, nucleotides, oligonucleotides) tailored to investigate and manipulate complex cellular processes in in vitro and in vivo biological systems. During the European meet-up hosted by the iGEM Munich team, she presented her works on UAA and their use in enzymology. We then had the opportunity to meet Pr. Lang and receive her advices concerning our project, after showing her our poster.
This was a great boosting event for us. We liked her commenting “There’s no reason for it not to work”! Her comments on our general design also helped us progress further in our project.
Philippe Serp
Inorganic Chemistry, ENSIACET (Toulouse, France)
Philippe Serp is a researcher at the “Laboratoire de Chimie de Coordination» and professor at ENSIACET (National School of Engineering in Chemical and Technological Arts).
We met Mr. Serp to get advice on an idea we had about using graphene to functionalize cellulose. Thanks to his advices, we have been able to modify our project to optimize our chances of success. Initially starting with the use of oxidized graphene in our project, we have, on his advice, changed to non-oxidized graphene. He also helped us to optimize our functionalization protocols with the assistance of Jerome Volkman, an organic chemist. They were kind enough to allow us manipulating in his laboratory to functionalize graphene. Indeed, we succeeded in adding DBCO chemical groupement to graphene with their help. It is just a pity we did not have enough time to test graphene on cellulose and we deeply apologize to Philippe and his team for that.
Jeremy Esque
Modelling, LISBP (Toulouse, France)
Given the central role of the Cerberus linker in our project, it was crucial to modelize its biochemical structure. We soon discover this was not a trivial challenge since parts of the molecule was of unknown structure (AzF, the junctions between CBM3a and the other heads for instance). Fortunately, we had the assistance of Jeremy Esque, INRA Research Engineer at the Toulouse LISBP. Jeremy is a specialist in structural bioinformatics. Its competences in structural dynamics of biomolecules have been an invaluable asset to our project, especially for the in silico assessment of Cerberus stability.
Christine Lafforgue
Fermentation processes, LISBP (Toulouse, France)
Our first idea was to produce functionalized cellulose in vivo. So we aimed to produce Cerberus and its associated function by the yeast Pichia pastoris in bioreactor. We had long discussion about this with Christine Lafforgue. She is a specialist in fermentation process and her insight was invaluable to our project since it avoided us lot of difficulties. Indeed, it came out from our discussion that cellulose production in bioreactor was a very risky and required tangential flow filtration to maintain a productive system. We therefore reshaped our project and put most of our strain in a E. coli driven production with subsequent purification. In vivo production is still a possibility for Cerberus, but it was no more a priority and the results prove her right.
SCIENCE & COMICS
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RETROSPECTIVE OF COMICS : A MIRROR OF OUR SOCIETY
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SUPERHEROES, VILAINS AND SCIENCE
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Origin Stories
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Use of Superabilities
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Science & Responsabilities
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SCIENCE & FANTASY
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Perception of science altered by comics
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Science inspired by comics
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COMICS AND OUR HUMAN PRACTICES APPROACH
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No dogs were harmed over the course of this iGEM project.
The whole Toulouse INSA-UPS team wants to thank our sponsors, especially:
And many more. For futher information about our sponsors, please consult our Sponsors page.
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