Difference between revisions of "Team:Munich/chibiobrick1.html"

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<h3>2018/06/06 - 2018/06/26</h3>
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<h3><u>2018/06/06 - 2018/06/26</u></h3>
 
<h4>Redo: Assembling pSB1C3_Chi6 with A3 Assembly</h4>
 
<h4>Redo: Assembling pSB1C3_Chi6 with A3 Assembly</h4>
 
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Revision as of 10:10, 29 September 2018

2018/06/04

Assembling pSB1C3_Chi6 with A3 Assembly

Participants: Dominic
Protocols: restriction digest, Ligation, chem trafo, mini-prep, sequencing
Notes: digest with XbaI, SpeI
transformation of Dh5a
Results: no sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment.

2018/06/06 - 2018/06/26

Redo: Assembling pSB1C3_Chi6 with A3 Assembly

Participants: Dominic
Protocol: restriction digest, PCR purification, ligation, chem trafo, PCR, Agarose gel
Notes: digest with XbaI, SpeI
transformation of Dh5a
Results: No bands on the Gel. Colonies on the plate resulted from contamination.