Difference between revisions of "Team:Munich/thisisatest.html"
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<h4>Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</h4> | <h4>Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</h4> | ||
| + | <em>2018/05/07</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
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<td>Results:</td> | <td>Results:</td> | ||
| − | <td>no colonies</td> | + | <td>no colonies. we suspected electroporation to be a problem and tried chemical transformation of NEB Turbo next.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>Redo: Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</h4> | |
| − | + | <em>2018/05/18</em> | |
| − | <h4>Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering</h4> | + | |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
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<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td>chem Trafo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>Results:</td> | <td>Results:</td> | ||
| − | <td>no colonies</td> | + | <td>no colonies. We suspected the E. Coli NEB Turbo to be a problem and switched to E. Coli Dh5a as a cloning organism.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>Redo: Transforming E.Coli Dh5a to amplify plasmids for pRED/ET Engineering</h4> | |
| − | <h4>Transforming E.Coli | + | <em>2018/05/24</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
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</table> | </table> | ||
| − | + | <h4>Transforming E.Coli Dh5a to amplify plasmids for pRED/ET Engineering</h4> | |
| − | + | <em>2018/05/25</em> | |
| − | <h4>Transforming E.Coli | + | |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
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<td>Results:</td> | <td>Results:</td> | ||
| − | <td>no colonies</td> | + | <td>no colonies. because pRED/ET could not be transformed, we got readily transformed cells from PD Dr. Jürgen Lassak from the LMU</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | |||
<h4>DNA preparation for pRED/ET Engineering</h4> | <h4>DNA preparation for pRED/ET Engineering</h4> | ||
| + | <em>2018/05/26</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
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</div> | </div> | ||
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Revision as of 14:07, 29 September 2018
Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
2018/05/07| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | pkD3 contains resistance cassettes with FRT-sites for pRED engineering incubate pRED at 30°C because of temperature sensitive promoter pNPTS138-R6KT is for knock-ins via RecA Recombineering |
| Results: | no colonies. we suspected electroporation to be a problem and tried chemical transformation of NEB Turbo next. |
Redo: Transforming E.Coli NEB Turbo to amplify plasmids for pRED/ET Engineering
2018/05/18| Participants: | Dominic |
| Protocol: | chem Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter |
| Results: | no colonies. We suspected the E. Coli NEB Turbo to be a problem and switched to E. Coli Dh5a as a cloning organism. |
Redo: Transforming E.Coli Dh5a to amplify plasmids for pRED/ET Engineering
2018/05/24| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter, pKD3 contains resistance cassette flanked by FRT sites |
| Results: | no colonies |
Transforming E.Coli Dh5a to amplify plasmids for pRED/ET Engineering
2018/05/25| Participants: | Dominic |
| Protocol: | chem. Trafo |
| Notes: | pKD3 contains resistance cassette flanked by FRT sites |
| Results: | no colonies. because pRED/ET could not be transformed, we got readily transformed cells from PD Dr. Jürgen Lassak from the LMU |
DNA preparation for pRED/ET Engineering
2018/05/26| Participants: | Dominic |
| Protocol: | mini prep |
| Notes: | because pRED/ET could not be transformed, we got readily transformed cells from PD Dr. Jürgen Lassak from the LMU |
| Results: | pRED/ET: 37,5 ng/ul pNPTS138-R6KT: 60ng/ul |