Difference between revisions of "Team:Munich/engineering1.html"
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<h4>Transforming E. Coli Rosetta for pRED/ET engineering</h4> | <h4>Transforming E. Coli Rosetta for pRED/ET engineering</h4> | ||
| + | <em>2018/06/04</em> | ||
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<td>Results:</td> | <td>Results:</td> | ||
| − | <td>no colonies</td> | + | <td>no colonies. We decided to redo the experiment with another pRED plasmid from PD Dr. Sandra Sagredo from the Simmel lab.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>Redo: Transforming E. Coli Rosetta for pRED/ET engineering</h4> | |
| − | <h4>Transforming E. Coli Rosetta for pRED/ET engineering</h4> | + | <em>2018/06/08</em> |
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<td>Results:</td> | <td>Results:</td> | ||
| − | <td>no colonies</td> | + | <td>no colonies. We decided to redo the experiment by electroporation.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
<h3>6/12</h3> | <h3>6/12</h3> | ||
| − | <h4>Transforming E. Coli Rosetta for pRED/ET engineering</h4> | + | <h4>Redo:Transforming E. Coli Rosetta for pRED/ET engineering</h4> |
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<td>Results:</td> | <td>Results:</td> | ||
| − | <td>no colonies</td> | + | <td>no colonies. pRED/ET engineering seems to not work in Rosetta. we postponed this experiment to do it with PD Dr. Lassak at the LMU again.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
</div> | </div> | ||
Revision as of 14:19, 29 September 2018
Contents
Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/04
| Participants: | Dominic |
| Protocol: | chem. Trafo |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter |
| Results: | no colonies. We decided to redo the experiment with another pRED plasmid from PD Dr. Sandra Sagredo from the Simmel lab. |
Redo: Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/08
| Participants: | Dominic |
| Protocol: | chem. Trafo |
| Notes: | we used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN |
| Results: | no colonies. We decided to redo the experiment by electroporation. |
6/12
Redo:Transforming E. Coli Rosetta for pRED/ET engineering
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | we used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN |
| Results: | no colonies. pRED/ET engineering seems to not work in Rosetta. we postponed this experiment to do it with PD Dr. Lassak at the LMU again. |