Difference between revisions of "Team:Munich/growth.html"
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| − | + | <h4>Growth of msbB and RecBCD-Nuklease deficient E.Coli mutant strains</h4> | |
| − | + | <em>2018/08/28</em> | |
| − | <h4> | + | |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Katja</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
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<h4>preparing electro-competent cells of WT RecBCD und dRecB</h4> | <h4>preparing electro-competent cells of WT RecBCD und dRecB</h4> | ||
| + | <em>2018/08/31</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Katja</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<tr> | <tr> | ||
<td>Results:</td> | <td>Results:</td> | ||
| − | <td> | + | <td> PICcells are still viable after preparation, dRecB grows significantly slower than others</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>Transforming E. Coli dRecB with pSB1C3_RecBCD(WT)</h4> | |
| − | <h4>Transforming E.Coli dRecB with pSB1C3_RecBCD(WT)</h4> | + | <em>2018/09/03</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Katja</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td>epo trafo</td> | <td>epo trafo</td> | ||
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| − | |||
| − | |||
| − | |||
| − | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
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</table> | </table> | ||
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<h4>DNA midi prep of RecBCD(WT) plasmid from Dh5a</h4> | <h4>DNA midi prep of RecBCD(WT) plasmid from Dh5a</h4> | ||
| + | <em>2018/09/03</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Katja</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>DNA Midi prep | + | <td>DNA Midi-prep</td> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</tr> | </tr> | ||
</table> | </table> | ||
| − | + | <h4>Growth of RecB, RecD, RecBCD, msb�-B deficient E.Coli strains</h4> | |
| − | <h4> | + | <em>2018/09/14</em> |
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
| − | |||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Katja</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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</div> | </div> | ||
| + | </html> | ||
Revision as of 18:24, 29 September 2018
Growth of msbB and RecBCD-Nuklease deficient E.Coli mutant strains
2018/08/28| Participants: | Katja |
| Protocol: | Measuring optical density |
| Notes: | |
| Results: | PIC |
preparing electro-competent cells of WT RecBCD und dRecB
2018/08/31| Participants: | Katja |
| Protocol: | preparing electro-competent cells |
| Results: | PICcells are still viable after preparation, dRecB grows significantly slower than others |
Transforming E. Coli dRecB with pSB1C3_RecBCD(WT)
2018/09/03| Participants: | Katja |
| Protocol: | epo trafo |
| Results: | colonies |
DNA midi prep of RecBCD(WT) plasmid from Dh5a
2018/09/03| Participants: | Katja |
| Protocol: | DNA Midi-prep |
Growth of RecB, RecD, RecBCD, msb�-B deficient E.Coli strains
2018/09/14| Participants: | Katja |
| Protocol: | Measuring optical density |
| Notes: | |
| Results: | PIC |