Difference between revisions of "Team:iTesla-SoundBio/description"

Line 10: Line 10:
  
 
<style>
 
<style>
−
.center {
+
<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.3.1/jquery.min.js"></script>
 +
<script>
 +
$(document).ready(function(){
 +
  // Add smooth scrolling to all links
 +
  $("a").on('click', function(event) {
 +
 
 +
    // Make sure this.hash has a value before overriding default behavior
 +
    if (this.hash !== "") {
 +
      // Prevent default anchor click behavior
 +
      event.preventDefault();
 +
 
 +
      // Store hash
 +
      var hash = this.hash;
 +
 
 +
      // Using jQuery's animate() method to add smooth page scroll
 +
      // The optional number (800) specifies the number of milliseconds it takes to scroll to the specified area
 +
      $('html, body').animate({
 +
        scrollTop: $(hash).offset().top
 +
      }, 800, function(){
 +
 
 +
        // Add hash (#) to URL when done scrolling (default click behavior)
 +
        window.location.hash = hash;
 +
      });
 +
    } // End if
 +
  });
 +
});
 +
</script>
 +
 
 +
<style>
 +
body {
 +
    font-family: "Lato", sans-serif;
 +
}
 +
 
 +
.sidenav {
 +
    height: 80%;
 +
    width: 180px;
 +
    position: fixed;
 +
    z-index: 1;
 +
    top: 0;
 +
    left: 0;
 +
    background-color: #111;
 +
    overflow-x: hidden;
 +
    padding-top: 200px;
 +
}
 +
 
 +
.sidenav a {
 +
    padding: 5px 5px 5px 5px;
 +
    text-decoration: none;
 +
    font-size: 20px;
 +
    color: #818181;
 +
    display: block;
 +
}
 +
 
 +
.sidenav a:hover {
 +
    color: #f1f1f1;
 +
}
 +
 
 +
.main {
 +
    margin-left: 180px; /* Same as the width of the sidenav */
 +
    padding: 0px 10px;
 +
    font-family: arial;
 +
    font-size: 20px;
 +
    padding: 30px
 +
}
 +
@media screen and (max-height: 450px) {
 +
    .sidenav {padding-top: 15px;}
 +
    .sidenav a {font-size: 18px;}
 +
}
 +
.graphicsum {
 
   width: 60%;
 
   width: 60%;
 
   margin: auto;
 
   margin: auto;
Line 72: Line 140:
  
 
</style>
 
</style>
−
<div class="center">
+
</style>
 +
 
 +
 
 +
<div class="sidenav">
 +
<a href="#graphicsum">Top</a>
 +
<br>
 +
<a href=#sum>Summary</a>
 +
<br>
 +
<a href=#1>Horseshoe Crabs</a>
 +
<br>
 +
<a href=#2>Current Practice</a>
 +
<br>
 +
<a href=#3>LAL and Factor C</a>
 +
<br>
 +
<a href=#4>Project Goal</a>
 +
<br>
 +
<a href=#5>Project Design</a>
 +
<br>
 +
<a href=#6>What's next</a>
 +
<br>
 +
<a href=#bibli>Bibliography</a>
 +
</div>
 +
<div class="graphicsum">
 
</div>
 
</div>
 
<body>
 
<body>

Revision as of 00:05, 30 September 2018

Safety Protocols
InterLab
Team Members Attributions
Human Practices Collaborations
Project Description Design Results Biobrick Parts Lab Notebook
iT-SB 2018 iT-SB 2017

Build the change

You want to see in the world

Top
Summary
Horseshoe Crabs
Current Practice
LAL and Factor C
Project Goal
Project Design
What's next
Bibliography
DID YOU KNOW? Every year, hundreds of thousands of horseshoe crabs are caught each year and drained of up to to 30% of their blood. Why? To collect LAL, a chemical that is crucial in the detection of endotoxins in everything from drugs to medical equipment

Construct design: The goal for our project is to insert Factor C gene into the chromosomal DNA of B. Subtilis, and have it express this Factor C protein. At the same time, to fulfill iGEM competition requirements, we also need to insert Factor C gene into E.coli plasmid pSB1C3 and submit it as a biobrick for iGEM competition. We decided to insert Factor C gene into E.coli plasmid pSB1C3 first; then, integrate Factor C gene into the chromosomal DNA of B. Subtilis using pAX01 integration factor (a B. Subtilis specific plasmid). Biobrick Factor C in E.coli pSB1C3 We couldn't order Factor C from factory because it is too large (about 3000 base pairs https://benchling.com/itesla-soundbio/f/gPUeHZXf-drafts/seq-Jq87odxl-psb1c3factor-c/edit), so we order it as two fragments, FC1 and FC2. First, insert FC1 into pSB1C3. Then, insert FC2 into the plasmid, too. This pSB1C3 with full Factor C will be submitted for biobrick. Integration Factor C in B. Subtilis Chromosomal DNA. We then digest full Factor C from Biobrick, and insert it into pAX01, a B. Subtilis integration factor. Then, we transform B. Subtilis with pAX01. After plasmid pAX01 enters B. Subtilis, there is a chance that it will integrate into chromosomal DNA. Expression Factor C protein extract B. Subtilis are used to express Factor C because they are gram positive and will not damage the Factor C protein. In contrast, E. coli is gram negative and will cleave FC protein. After B. Subtilis express and produce Factor C, we can then extract Factor C protein for further experiment.