Difference between revisions of "Team:SCAU-China/Safety"

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       <div class="DBoard" id="title">     
 
       <div class="DBoard" id="title">     
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       Safety
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       Lab Safety
 
       </div>
 
       </div>
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      <div class="DBoard" id="article1"><!-- 展板 -->
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        <p>In many of the world's countries drought, chronic water shortage is a fact of life. Therefore, to find a efficient way
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        to preserve water in the arid and semiarid environments is imminent.</p>
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       <p>The use of cyanobacteria as soil conditioners has been studied intermittently for many decades. Some of the documented direct effects of cyanobacterial inoculation are related to soil stabilization and improvement, enrichment in nutrients and increase in moisture content. Recently inoculation approaches have proven to be effective tools also for triggering land rehabilitation in arid and semiarid environments.  
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      <div class="DBoard" id="title" style="height:40px;width:400px;font-size:20px;line-height:40px;text-align:center;">   
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    Lab
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      </div>
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<div class="DBoard" id="article1" style="height:300px;"><!-- 展板 -->
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       <p>Our lab is classified as Biosafety Level 1, which means the lab uses microbes that pose little to no risk to healthy individuals. Hazardous chemicals and solutions are used at a minimal level and in well ventilated areas such as fume hoods in the SCAU research lab.  
  
 
</p>
 
</p>
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       <p>Bacterial cellulose is a strong and ultrapure form of cellulose produced naturally by several species of the Acetobacteraceae. Bacterial cellulose produced by Gluconacetobacter xylinus possesses an abundance of desirable properties, its special 3D structure make it has a good water-retaining property.</p>
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       </div>
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      <p>Our project try to investigates the potential for using cellulose synthesized by cyanobacteria as an alternative.Makes our product has both the advantages of cyanobacterial crust, but also have the ability to produces high water-retention bacterial cellulose.To produce BC in Cyanobacteria, a suite of genes involved with cellulose synthesis in G. xylinus will be transferred to into the terricolous, unicellular cyanobacterium,  Scytonema javanicum and Microcolus vaginatus.</p>
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 +
<div class="DBoard" id="title" style="height:40px;width:400px;font-size:20px;line-height:40px;text-align:center;">   
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    Training & Rules
 
       </div>
 
       </div>
 +
<div class="DBoard" id="article1"><!-- 展板 -->
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      <p>In order to ensure that the experimental operations are safe to humans and the environment, we trained all our team members in experimental operations. And every team member has been asked to take a series of safety courses including biological safety, chemical safety and general safety which are provided by the university.
 +
 
  
−
   
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</p>
 +
<p>Additionally , we followed a lab safety handbook to ensure that we use chemicals in a safe way.</p>
 +
      </div>
  
  
 +
<div class="DBoard" id="title" style="height:40px;width:400px;font-size:20px;line-height:40px;text-align:center;">   
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    Reagent
 +
      </div>
 +
<div class="DBoard" id="article1"><!-- 展板 -->
 +
      <p>We use the chloroform and phenol when we extract the genome and plasmid , and both of the drug are poisonous. But we treated them very careful, for example, we weared the gloves to protect ourselves and all toxic and organic reagents are placed in special recycling bins.
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 +
 +
 +
</p>
 +
      </div>
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 +
<div class="DBoard" id="title" style="height:40px;width:400px;font-size:20px;line-height:40px;text-align:center;">   
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  Equipment
 +
      </div>
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<div class="DBoard" id="article1" style="height:400px;"><!-- 展板 -->
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      <p>1. We use the wood toothpick instead of needles which may stick ourselves during picking E.coli colony.
 +
</p>
 +
<p>
 +
2. We wrap the broken glass crucible in plastic before discarding it.
 +
</p>
 +
<p>3. All of used disposable laboratory consumables will be brought together and then recycled by a dedicated person.</p>
 +
      </div>
 +
 +
 +
 +
 +
<div class="DBoard" id="title" style="height:40px;width:400px;font-size:20px;line-height:40px;text-align:center;">   
 +
  Organism Safety
 +
      </div>
 +
<div class="DBoard" id="article1" style="height:400px;"><!-- 展板 -->
 +
<p>The chassis that we used belongs to the P1 level according to the classification of biosafety.</p>
 +
      <p>1. We use E.coli strains termed DH5alpha and DH10B for the molecular cloning. They are non-pathogenic and have been widely used for gene cloning in many labs around the world. It has been shown that they are barely toxic to researchers when handled properly. We have participated in the biological safety course provided by our university and been well trained for handling the E.coli safely.
 +
</p>
 +
<p>
 +
In addition, Acetobacter xylinum and cyanobacteria (Synechocystis sp., Scytonema javanicum and Microcoleus vaginatus) are safe microorganisms. and they can't live without of the culture medium and do not have any harmful effects on human health.
 +
</p>
 +
<p>3. Finally, all organisms are sterilized prior to being discarded.
 +
 +
</p>
 +
      </div>
 
      
 
      
 
       <p>School's name:SCAU</p>
 
       <p>School's name:SCAU</p>

Revision as of 08:33, 1 October 2018

SCAU-2018
Lab Safety
Lab

Our lab is classified as Biosafety Level 1, which means the lab uses microbes that pose little to no risk to healthy individuals. Hazardous chemicals and solutions are used at a minimal level and in well ventilated areas such as fume hoods in the SCAU research lab.

Training & Rules

In order to ensure that the experimental operations are safe to humans and the environment, we trained all our team members in experimental operations. And every team member has been asked to take a series of safety courses including biological safety, chemical safety and general safety which are provided by the university.

Additionally , we followed a lab safety handbook to ensure that we use chemicals in a safe way.

Reagent

We use the chloroform and phenol when we extract the genome and plasmid , and both of the drug are poisonous. But we treated them very careful, for example, we weared the gloves to protect ourselves and all toxic and organic reagents are placed in special recycling bins.

Equipment

1. We use the wood toothpick instead of needles which may stick ourselves during picking E.coli colony.

2. We wrap the broken glass crucible in plastic before discarding it.

3. All of used disposable laboratory consumables will be brought together and then recycled by a dedicated person.

Organism Safety

The chassis that we used belongs to the P1 level according to the classification of biosafety.

1. We use E.coli strains termed DH5alpha and DH10B for the molecular cloning. They are non-pathogenic and have been widely used for gene cloning in many labs around the world. It has been shown that they are barely toxic to researchers when handled properly. We have participated in the biological safety course provided by our university and been well trained for handling the E.coli safely.

In addition, Acetobacter xylinum and cyanobacteria (Synechocystis sp., Scytonema javanicum and Microcoleus vaginatus) are safe microorganisms. and they can't live without of the culture medium and do not have any harmful effects on human health.

3. Finally, all organisms are sterilized prior to being discarded.

School's name:SCAU

Member's name:SCAU

Designed by:SCAU