Team OUC-China: MainjQuery UI 折叠面板（Accordion） - 非自动高度
Notebook
Week 1 2018.2.4-2018.2.10
1. Completed the team registration.
2. Learnt about synthetic biology by reading papers and wikis.
3. Tried to set up our project.
4. The iGEM team from SDU-China came to our meeting room for communication.
Week 2 2018.2.11-2018.2.17
1. Learnt about synthetic biology by reading papers and wikis.
2. Tried to set up our project.
3. Looked for the PI of our project.
Week 3 2018.2.18-2018.2.24
1. Sorted out the previous projects, and made the first selection according to the feasibility of each project.
2. The principles of all projects had been further explored.
3. We asked our instructor for advice.
4. Began to learn microbial experiment operation, and everyone in the team carried out the test of experiment operation.
Week 4 2018.2.25-2018.3.3
1. Started communication and collaboration with iGEM teams in other schools.
2. Began to familiarize ourselves with laboratory equipment and other arrangements.
3. Had applied for the 5th Conference of China iGEMer Community（CCiC）.
4. Set up some more projects.
Week 5 2018.3.4-2018.3.10
1. The previous project was classified again, and our team members were divided into different groups to dig deeper into the principles of the project.
2. Started learning molecular experimentation.
3. Started preparing the bid report of the 5th Conference of China iGEMer Community（CCiC）.
Week 6 2018.3.11-2018.3.17
1. Had temporarily identified two alternative projects and confirmed their circuit, components, related reagents as well as equipment, analyzed their feasibility in detail, continuously filled and enriched the project content, and tried to design experiments.
2. Contacted the authors of the project-related papers, inquired about the details, and received many suggestions from the original paper’s team.
3. Asked many teachers and our seniors at the same time, and got their advice.
4. We were learning about molecular experimentation.
5. Introduced team status, project progress and other issues to our PI.
6. Set daily management rules for our team.
Week 7 2018.3.18-2018.3.24
1. Referred to the opinions of the teachers and members of the previous team, finally chose one of the two projects according to their feasibility.
2. Purchased the required strains: RP6666 and RP437.
3. Contacted the equipment needed for our project.
4. Sorted out the materials of the 5th Conference of China iGEMer Community (CCiC) and communicated with our school, finally won the support of the school.
Week 8 2018.3.25-2018.3.31
1. Preserved RP6666 and RP437.
2. Modified the existing project, proposed a new project.
3. Made a detailed exploration of the principle, circuit, parts and equipment of the project and sorted out a file package.
4. Got in touch with the original author of the paper, Mr. Du pei, and got his help.
Week 9 2018.4.1-2018.4.7
1. Sent the package of documents to PI, teachers and seniors, and listened to their opinions to constantly improve and perfect the project.
2. Started trying to contact teachers who could provide guidance or help for our projects（For example, teachers from the Chinese academy of sciences）.
3. We were divided into two groups, one responsible for experimental design, the other responsible for circuit design, determining the detailed details of the project, and trying to build the model.
4. Submitted the CCiC bid report.
Week 10 2018.4.8-2018.4.14
1. Communicated with Mr. Du pei and got the plasmid we need.
2. The first validation experiment was conducted with plasmid.
3. In the plasmid transformation experiment, our bacteria did not grow well on the plate. We conducted a detailed analysis on the operation of the experiment, and sought the help of the teacher, formulated the remedial plan.
4. The bid for the 5th Conference of China iGEMer Community (CCiC) ended in the voting, and we will hold CCiC as the co-organizer.
5. The division of duties of the team was determined.
6. Contacted biotechnology companies to sequence plasmids and bought new laboratory reagents.
Week 11 2018.4.15-2018.4.21
1. With our persistence and efforts, we successfully enriched the plasmid.
2. Commissioned the company to synthesize the circuit.
Week 12 2018.4.22-2018.4.28
1. Measured the first system fluorescence with an microplate reader, but the data was not very good.
2. Gave the first popular science lecture on synthetic biology.
3. Measured the growth curves of three strains of the first system.
Week 13 2018.4.29-2018.5.5
1. Asked the teacher if cultivating bacteria without IPTG whether could form a control group.
2. The control group was redesigned.
3. Gave our first intramural lecture on synthetic biology.
4. Designed the experimental scheme of Interlab.
Week 14 2018.5.6-2018.5.12
1. Started building a new circuit to complement our project.
2. Analyzed the experimental scheme of measuring fluorescence last time, summarized the problem, replaced the M9 medium, remeasured the first system, and obtained better experimental data.
3. We are going to design a game related to our project, and started the game art design.
4. The preliminary experiment of Interlab was carried out.
5. The primer design of the standardized experiment was carried out.
Week 15 2018.5.13-2018.5.19
1. Began to design the medium of the motA circuit for detecting the motility.
2. Identified the primers for standardized experiments and commissioned the company to synthesize them.
3. Completed the pre-experiment of the project.
4. Designed a formal experimental scheme.
5. Started writing part descriptions.
6. Started writing the project overview.
7. Started the recruitment of the next team.
8. Built the basic model of the first system.
Week 16 2018.5.20-2018.5.26
1. We submitted the application for the summer social practice project to our school, and we want to use the platform of the school to promote synthetic biology to more people.
2. Did a preliminary design of the web page.
3. Finished the first draft of our cartoon.
Week 17 2018.5.27-2018.6.2
1. Designed the uniforms of our team.
2. Performed transformation of four mutant enzyme plasmids.
3. Carried out the pre-experiment of the standardized experiment.
4. Completed the fluorescence measurement of four kinds of mutant Csy4 enzyme combined with wild type of hairpin.
Week 18 2018.6.3-2018.6.9
1. Applied for visa.
2. Standardized the wild type of Csy4 enzyme.
Week 19 2018.6.10-2018.6.16
1. Contacted many biotechnology companies in Qingdao.
2. Our summer social practice program was supported by the school and we planned to better promote synthetic biology.
Week 20 2018.6.17-2018.6.23
1. Sorted out what we have done so far and put forward future arrangements and plans.
