Difference between revisions of "Team:Goettingen/Notebook/biobricks May"

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                     <td>PCR, cloning</td>
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                     <td>no mutations</td>
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Revision as of 06:42, 2 October 2018

All basic parts were created via standard cloning techniques and methods are described <a href="https://2018.igem.org/Team:Goettingen/Experiments" target="_blank">here</a>. The composite parts were constructed, using the 3A-assembly, which is also described <a href="https://2018.igem.org/Team:Goettingen/Experiments" target="_blank">here</a>. The following table gives an overview about our parts.

Part What? How? Sequenced? Ok?
BB1 gltT CCR, cloning no mutations ✔
BB2 gltP PCR, cloning no mutations ✔
BB3 Palf4 PCR, cloning no mutations ✔
BB4 RBS PCR, cloning no mutations ✔
BB5 RBS-gltT 3A-assembly ✘
BB6 Palf4-RBS 3A-assembly no mutations ✔
BB7 Palf4-RBS-gltT 3A-assembly no mutations ✘
BB8 aroE CCR, cloning no mutations ✔
BB9 aroA PCR, cloning no mutations ✔
BB10 PtrpP PCR, cloning no mutations ✔
BB11 gat PCR, cloning no mutations ✔
BB12 aroA* PCR, cloning no mutations ✔
BB13 Palf4-RBS-gat 3A-assembly ✘
BB14 Palf4-RBS-aroA* 3A-assembly ✘
BB15 Pcomm not yet ✘
BB16 Strep-tag-aroE PCR, cloning ✘
BB17 Strep-tag-aroA PCR, cloning ✘
BB18 PyciC–xylR 3A-assembly ✘
BB19 PyciAB–xylR 3A-assembly ✘
BB20 PyciC PCR, cloning ✘
BB21 PyciAB PCR, cloning ✘
BB22 xylR PCR, assembly ✘