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Revision as of 04:24, 4 October 2018
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Microbial Symphony
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<section>
Constitutive Expression
The gene of interest is hooked up to a constitutive promoter, resulting in constant expression. The primary benefit of this "system" is its inherent simplicity - expression will always just occur. However, when optimizing yields, a flaw in this system becomes evident; low yields.
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The Problem
In biomanufacturing, there are two common systems for inducing expression of some gene of interest.
</section> <section>
Inducible Expression
A better solution resulting in higher yields involves hooking the gene in question up to an inducible promoter. This system gives the culture time to grow without gene expression; growth occurs an at an accelerated rate because all metabolic activity is focused on growth. Once the culture has reached an optimal cell density, an artificial inducer can be added, switching on production of the gene in question.
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<section>
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