For secretion peptides….

For our project we will model the rates of fluorescence at which our secretion peptides express in response to variations in nitrosative stress. For this, we will use the following equation for our mathematical model:

Where P1 represents the fluorescence of the secretion peptides and t the time; n is for the number of copies of mRNA, k1 expresses the specific rate of production per copy of mRNA and k2 represents the specific rate of secretion per unit of P1.

Our model allowed us to construct a graphic relation between the fluorescence of our secretion peptides and the different concentrations of nitrate. Experiments were made in continuous culture, in which we added different concentrations of nitrate (CONCENTRACIONES) until steady state was reached and we measured the fluorescence with a plate reader (MODELO, AÑO…). The continuous system served to establish the secretion efficiency.

In general, this model was used to see the role played by the different concentrations of nitrate and thus be able to design an optimized system to stimulate the production of our proteins of interest .

We obtained the follow data showed in Table X, in Figure X we present the resulting curve of the fluorescence vs. the concentration of nitrate.

Table X. Data obtained at (CONDICIONES) of the fluorescence of the secretion peptides

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Figure X. Graphic of the fluorescence of the secretion peptides against different concentrations of nitrate at (CONDICIONES)

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