Difference between revisions of "Team:TecMonterrey GDL/Constructs"
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<h3> <span>Composite part Plan A: </span> </h3> | <h3> <span>Composite part Plan A: </span> </h3> | ||
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<img src="https://static.igem.org/mediawiki/2018/b/bf/T--TecMonterrey_GDL--ConstructEColi.png" class="my-image" > | <img src="https://static.igem.org/mediawiki/2018/b/bf/T--TecMonterrey_GDL--ConstructEColi.png" class="my-image" > | ||
<span> This construct is optimized for E.coli BL21, it constitutes our “Plan A” for our psychobiotic, which consists of producing the soluble form gp130 attached to novel signal peptides, which will allow the secretion of the sgp130 to the extracellular media. In order for our construct to work the IL-6 will bind to its receptor also known as gp80, these dimer will then bind to the produced soluble sgp130 which will form a highly stable trimer that sequesters IL-6, effectively inhibiting IL-6 trans-signaling, the inflammatory signalization. </span> | <span> This construct is optimized for E.coli BL21, it constitutes our “Plan A” for our psychobiotic, which consists of producing the soluble form gp130 attached to novel signal peptides, which will allow the secretion of the sgp130 to the extracellular media. In order for our construct to work the IL-6 will bind to its receptor also known as gp80, these dimer will then bind to the produced soluble sgp130 which will form a highly stable trimer that sequesters IL-6, effectively inhibiting IL-6 trans-signaling, the inflammatory signalization. </span> | ||
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Revision as of 03:20, 5 October 2018
Construct Design
Composite part Plan A:
This construct is optimized for E.coli BL21, it constitutes our “Plan A” for our psychobiotic, which consists of producing the soluble form gp130 attached to novel signal peptides, which will allow the secretion of the sgp130 to the extracellular media. In order for our construct to work the IL-6 will bind to its receptor also known as gp80, these dimer will then bind to the produced soluble sgp130 which will form a highly stable trimer that sequesters IL-6, effectively inhibiting IL-6 trans-signaling, the inflammatory signalization.