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<h4 align="left"> Composite part Plan A: </h4> | <h4 align="left"> Composite part Plan A: </h4> | ||
<h4 align="right"> For L. rhamnosus <h4> | <h4 align="right"> For L. rhamnosus <h4> | ||
+ | <span> This is the same “Plan A” for E. coli but adapted to Lactobacillus rhamnosus GG, since it is a natural probiotic organism. Plan A which consists of producing the soluble form gp130 attached to novel signal peptides for secretion to the media, this proteins will form a dimer with gp80 found in the gut, which will then attach to IL-6 and form a stable trimer capable of blocking interleukin inflammatory signalization. The construct is codon optimized for L. rhamnosus GG, it has promoters, RBS and terminators that have been used before for L. rhamnosus. It also contains two restriction sites, between the iGEM prefix and suffix, to ligate the Lactobacillus ORI site. | ||
+ | </span> | ||
</div> | </div> | ||
<div> | <div> |
Revision as of 22:51, 5 October 2018
Construct Design ![decoration1](https://static.igem.org/mediawiki/2018/3/3a/T--TecMonterrey_GDL--decoration1.png)
Composite part Plan A:
![](https://static.igem.org/mediawiki/2018/b/bf/T--TecMonterrey_GDL--ConstructEColi.png)
![](https://static.igem.org/mediawiki/2018/9/91/T--TecMonterrey_GDL--ConstructIL-6.png)
![](https://static.igem.org/mediawiki/2018/f/f1/T--TecMonterrey_GDL--Construct3.png)
![](https://static.igem.org/mediawiki/2018/e/ea/T--TecMonterrey_GDL--Construct4.png)
References: [1] Müller-Newen, G., Küster, A., Hemmann, U., Keul,R., Horsten, U., Martens, A., Graeve, L., Wijdenes, J., Heinrich, P. (1998) Soluble IL-6 Receptor Potentiates the Antagonistic Activity of Soluble gp130 on IL-6 Responses. The Journal American of Association Immunology. ISSN: 1550-6606. [2] Han,S., Machhi, S., Berge,H., Xi, G., Linke, T., and Schoner, R. (2017). Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli. AMB Express. Doi: https://doi.org/10.1186/s13568-017-0394-1
Composite part Plan B (E. coli BL21):
![](https://static.igem.org/mediawiki/2018/9/91/T--TecMonterrey_GDL--ConstructIL-6.png)
![](https://static.igem.org/mediawiki/2018/b/bf/T--TecMonterrey_GDL--ConstructEColi.png)
![](https://static.igem.org/mediawiki/2018/f/f1/T--TecMonterrey_GDL--Construct3.png)