Difference between revisions of "Template:Virginia/InterLab"

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<h1 id="description">Description</h1>
+
<h1 id="interlab">InterLab</h1>
−
<p>Our Data!</p>
+
<p>We did this year&#39;s iGEM InterLab study!<br>Our original Excel file can be downloaded here.<br>Here is the protocol that we followed.    </p>
 +
<h2 id="od600-reference-point-ludox-protocol">OD600 Reference Point - LUDOX Protocol</h2>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th></th>
 +
        <th>LUDOX CL-X</th>
 +
        <th>H2O</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Replicate 1</td>
 +
        <td>0.113</td>
 +
        <td>0.087</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Replicate 2</td>
 +
        <td>0.105 </td>
 +
        <td>0.085</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Replicate 3</td>
 +
        <td>0.119 </td>
 +
        <td>0.084</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Replicate 4</td>
 +
        <td>0.103 </td>
 +
        <td>0.085</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Arith. Mean</td>
 +
        <td>0.110</td>
 +
        <td>0.085</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Corrected Abs600</td>
 +
        <td>0.025 </td>
 +
        <td></td>
 +
    </tr>
 +
    <tr>
 +
        <td>Reference OD600</td>
 +
        <td>0.063</td>
 +
        <td> </td>
 +
    </tr>
 +
    <tr>
 +
        <td>OD600/Abs600</td>
 +
        <td>2.545 </td>
 +
        <td></td>
 +
    </tr>
 +
</table>
 +
 
 +
<h2 id="particle-standard-curve-microsphere-protocol">Particle Standard Curve: Microsphere Protocol</h2>
 +
<p>Need to color code but:
 +
Abs600 measurements are blue cells.
 +
Gold cells are calculated. </p>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Number of Particles</th>
 +
        <th>2.35E+08</th>
 +
        <th>1.18E+08</th>
 +
        <th>5.88E+07</th>
 +
        <th>2.94E+07</th>
 +
        <th>1.47E+07</th>
 +
        <th>7.35E+06</th>
 +
        <th>3.68E+06</th>
 +
        <th>1.84E+06</th>
 +
        <th>9.19E+05</th>
 +
        <th>4.60E+05</th>
 +
        <th>2.30E+05</th>
 +
        <th>0</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Replicate 1</td>
 +
        <td>0.534</td>
 +
        <td>0.337</td>
 +
        <td>0.211</td>
 +
        <td>0.148</td>
 +
        <td>0.124</td>
 +
        <td>0.102</td>
 +
        <td>0.094</td>
 +
        <td>0.091</td>
 +
        <td>0.089</td>
 +
        <td>0.089</td>
 +
        <td>0.088</td>
 +
        <td>0.087</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Replicate 2</td>
 +
        <td>0.538</td>
 +
        <td>0.329</td>
 +
        <td>0.204</td>
 +
        <td>0.145</td>
 +
        <td>0.117</td>
 +
        <td>0.102</td>
 +
        <td>0.099</td>
 +
        <td>0.092</td>
 +
        <td>0.089</td>
 +
        <td>0.089</td>
 +
        <td>0.086</td>
 +
        <td>0.085</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Replicate 3</td>
 +
        <td>0.543</td>
 +
        <td>0.332</td>
 +
        <td>0.209</td>
 +
        <td>0.145</td>
 +
        <td>0.116</td>
 +
        <td>0.107</td>
 +
        <td>0.093</td>
 +
        <td>0.090</td>
 +
        <td>0.088</td>
 +
        <td>0.089</td>
 +
        <td>0.086</td>
 +
        <td>0.083</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Replicate 4</td><br>        <td>0.545</td>
 +
        <td>0.314</td>
 +
        <td>0.208</td>
 +
        <td>0.143</td>
 +
        <td>0.119</td>
 +
        <td>0.102</td>
 +
        <td>0.092</td>
 +
        <td>0.089</td>
 +
        <td>0.089</td>
 +
        <td>0.089</td>
 +
        <td>0.086</td>
 +
        <td>0.083</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Arith. Mean</td><br>        <td>0.540</td>
 +
        <td>0.328</td>
 +
        <td>0.208</td>
 +
        <td>0.145</td>
 +
        <td>0.119</td>
 +
        <td>0.103</td>
 +
        <td>0.095</td>
 +
        <td>0.091</td>
 +
        <td>0.089</td>
 +
        <td>0.089</td>
 +
        <td>0.087</td>
 +
        <td>0.085</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Arith. Std.Dev.    </td>
 +
        <td>0.005</td>
 +
        <td>0.010</td>
 +
        <td>0.003</td>
 +
        <td>0.002</td>
 +
        <td>0.004</td>
 +
        <td>0.003</td>
 +
        <td>0.003</td>
 +
        <td>0.001</td>
 +
        <td>0.001</td>
 +
        <td>0.000</td>
 +
        <td>0.001</td>
 +
        <td>0.002</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Arith. Net Mean</td><br>        <td>0.456</td>
 +
        <td>0.244</td>
 +
        <td>0.124</td><br>        <td>0.061</td>
 +
        <td>0.035</td>
 +
        <td>0.019</td>
 +
        <td>0.010</td>
 +
        <td>0.006</td>
 +
        <td>0.004</td>
 +
        <td>0.004</td>
 +
        <td>0.002</td><br>        <td></td>
 +
    </tr>
 +
</table>
 +
 
 +
<p><img src="https://static.igem.org/mediawiki/2018/f/fa/T--Virginia--2018_ParticleStandardCurve.png" alt="ParticleStandardCurve">
 +
<img src="https://static.igem.org/mediawiki/2018/6/63/T--Virginia--2018_ParticleStandardCurve_log.png" alt="ParticleStandardCurve_log"></p>
 +
<h3 id="particles-od">Particles/OD</h3>
 +
<p>Note: Final scaling level determined from medium-high points likely to be less impacted by saturation or pipetting error. If needed, you can shift which points are used, but it is likely better to correct instrument settings and protocol.</p>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Number of Particles</th><br>        <th>2.35E+08</th>
 +
        <th>1.18E+08</th>
 +
        <th>5.88E+07</th>
 +
        <th>2.94E+07</th>
 +
        <th>1.47E+07</th>
 +
        <th>7.35E+06</th>
 +
        <th>3.68E+06</th>
 +
        <th>1.84E+06</th>
 +
        <th>9.19E+05</th>
 +
        <th>4.60E+05</th>
 +
        <th>2.30E+05</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Mean particles / Abs600    </td>
 +
        <td>5.17E+08</td>
 +
        <td>4.83E+08</td>
 +
        <td>4.76E+08</td>
 +
        <td>4.84E+08</td>
 +
        <td>4.26E+08</td>
 +
        <td>3.92E+08</td>
 +
        <td>3.68E+08</td>
 +
        <td>3.06E+08</td>
 +
        <td>2.16E+08</td>
 +
        <td>1.02E+08</td>
 +
        <td>1.15E+08</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Mean of med-high levels:</td>
 +
        <td></td>
 +
        <td>4.52E+08</td>
 +
        <td></td>
 +
        <td></td>
 +
        <td></td>
 +
        <td></td>
 +
        <td></td>
 +
        <td></td>
 +
        <td></td>
 +
        <td></td>
 +
        <td></td>
 +
    </tr>
 +
</table>
 +
 
 +
<h3 id="cospheric-monodisperse-silica-microspheres-0-961um-diameter">Cospheric Monodisperse Silica Microspheres 0.961um diameter</h3>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Spheres/gram</th><br>        <th>Grams/mL</th>
 +
        <th>Spheres/0.55mL</th>
 +
        <th>Resuspend volume mL:</th>
 +
        <th>Dilution X:</th>
 +
        <th>Total volume mL:</th>
 +
        <th>Particles/mL:</th>
 +
        <th>Well volume (mL)</th>
 +
        <th>Initial particles:</th>
 +
    </tr>
 +
    <tr>
 +
        <td>1.20E+12</td>
 +
        <td>1.8</td>
 +
        <td>1.19E+12</td>
 +
        <td>2.55</td>
 +
        <td>100</td>
 +
        <td>255</td>
 +
        <td>4.71E+09</td>
 +
        <td>0.1</td>
 +
        <td>4.71E+08</td>
 +
    </tr>
 +
</table>
 +
 
 +
 
 +
<h2 id="raw-plate-reader-measurements">Raw Plate Reader Measurements</h2>
 +
<h3 id="fluorescence-raw-readings-">Fluorescence Raw Readings:</h3>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 0:    </th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
        <th>LB + Chlor (blank)</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td><br>        <td>124.47</td>
 +
        <td>172.15</td>
 +
        <td>136.79</td>
 +
        <td>137.65</td>
 +
        <td>115.66</td>
 +
        <td>132.36</td>
 +
        <td>127.35</td>
 +
        <td>135.34</td>
 +
        <td>112.83</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>121.01</td>
 +
        <td>142.23</td>
 +
        <td>113.72</td>
 +
        <td>130.07</td>
 +
        <td>93.041</td>
 +
        <td>106.03</td>
 +
        <td>104.61</td>
 +
        <td>113.2</td>
 +
        <td>98.307</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td><br>        <td>123.38</td><br>        <td>148.37</td><br>        <td>112.73</td><br>        <td>131.37</td><br>        <td>90.708</td><br>        <td>106.35</td><br>        <td>104.49</td><br>        <td>113.95</td><br>        <td>95.141</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td><br>        <td>119.97</td>
 +
        <td>141.57</td>
 +
        <td>113.49</td>
 +
        <td>124.8</td>
 +
        <td>93.35</td>
 +
        <td>107.29</td>
 +
        <td>102.57</td>
 +
        <td>111.37</td>
 +
        <td>96.012</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>121.32</td>
 +
        <td>134.7</td>
 +
        <td>116.21</td>
 +
        <td>169.04</td>
 +
        <td>93.865</td>
 +
        <td>110.22</td>
 +
        <td>152.05</td>
 +
        <td>111.67</td>
 +
        <td>99.513</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>134.51</td>
 +
        <td>141.18</td>
 +
        <td>115.5</td>
 +
        <td>131.01</td>
 +
        <td>94.94</td>
 +
        <td>107.69</td>
 +
        <td>124.91</td>
 +
        <td>114.24</td>
 +
        <td>96.457</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>130.47</td>
 +
        <td>141.41</td>
 +
        <td>114.54</td>
 +
        <td>130.7</td>
 +
        <td>92.262</td>
 +
        <td>111.95</td>
 +
        <td>128.96</td>
 +
        <td>115.03</td>
 +
        <td>98.671</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>129.77</td>
 +
        <td>160.97</td>
 +
        <td>142.19</td>
 +
        <td>130.7</td>
 +
        <td>112.12</td>
 +
        <td>128.64</td>
 +
        <td>155.08</td>
 +
        <td>129.88</td>
 +
        <td>122.04</td>
 +
    </tr>
 +
</table>
 +
 
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 6:    </th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
        <th>LB + Chlor (blank)</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td><br>        <td>147.58</td><br>        <td>923.47</td>
 +
        <td>481.57</td>
 +
        <td>768.79</td>
 +
        <td>145.84</td>
 +
        <td>158.29</td>
 +
        <td>196.74</td>
 +
        <td>472.33</td>
 +
        <td>130.21</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>135.57</td><br>        <td>868.37</td>
 +
        <td>443.8</td>
 +
        <td>771.52</td>
 +
        <td>133.54</td>
 +
        <td>150.48</td>
 +
        <td>174.77</td>
 +
        <td>442.69</td>
 +
        <td>108.87</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td><br>        <td>133.91</td>
 +
        <td>821.45</td>
 +
        <td>435.09</td>
 +
        <td>749.58</td>
 +
        <td>135.07</td>
 +
        <td>147.14</td>
 +
        <td>176.84</td>
 +
        <td>433.8</td>
 +
        <td>106.07</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td><br>        <td>131.16</td><br>        <td>825.34</td>
 +
        <td>424.75</td>
 +
        <td>702.31</td>
 +
        <td>136.56</td>
 +
        <td>145.48</td>
 +
        <td>178.85</td>
 +
        <td>435.43</td>
 +
        <td>101.34</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>129.25</td><br>        <td>736.73</td>
 +
        <td>506.04</td>
 +
        <td>721.65</td>
 +
        <td>130.79</td>
 +
        <td>152.77</td>
 +
        <td>263.05</td>
 +
        <td>404.78</td>
 +
        <td>105.13</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>129.93</td>
 +
        <td>746.95</td>
 +
        <td>522.99</td>
 +
        <td>752.02</td>
 +
        <td>130.12</td>
 +
        <td>154.08</td>
 +
        <td>274.44</td>
 +
        <td>99.78</td>
 +
        <td>102.2</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>134.9</td><br>        <td>737.42</td>
 +
        <td>519.61</td>
 +
        <td>760.98</td>
 +
        <td>129.32</td>
 +
        <td>162.53</td>
 +
        <td>277.69</td>
 +
        <td>390.5</td>
 +
        <td>104.1</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>136.44</td><br>        <td>824.81</td>
 +
        <td>585.7</td>
 +
        <td>749.59</td>
 +
        <td>145.38</td>
 +
        <td>171.03</td>
 +
        <td>319.39</td>
 +
        <td>429.11</td>
 +
        <td>122.55</td>
 +
    </tr>
 +
</table>
 +
 
 +
<h3 id="abs600-raw-readings">Abs600 Raw Readings</h3>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 0:    </th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
        <th>LB + Chlor (blank)</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td><br>        <td>0.114</td><br>        <td>0.112</td>
 +
        <td>0.112</td>
 +
        <td>0.116</td>
 +
        <td>0.115</td>
 +
        <td>0.113</td>
 +
        <td>0.11</td>
 +
        <td>0.112</td>
 +
        <td>0.097</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>0.113</td><br>        <td>0.112</td>
 +
        <td>0.108</td>
 +
        <td>0.108</td>
 +
        <td>0.109</td>
 +
        <td>0.109</td>
 +
        <td>0.106</td>
 +
        <td>0.112</td>
 +
        <td>0.097</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td><br>        <td>0.114</td><br>        <td>0.11</td>
 +
        <td>0.106</td>
 +
        <td>0.108</td>
 +
        <td>0.109</td>
 +
        <td>0.108</td>
 +
        <td>0.106</td>
 +
        <td>0.111</td>
 +
        <td>0.093</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td><br>        <td>0.114</td><br>        <td>0.11</td>
 +
        <td>0.106</td>
 +
        <td>0.108</td>
 +
        <td>0.109</td>
 +
        <td>0.107</td>
 +
        <td>0.105</td>
 +
        <td>0.109</td>
 +
        <td>0.095</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>0.115</td><br>        <td>0.114</td>
 +
        <td>0.108</td>
 +
        <td>0.123</td>
 +
        <td>0.111</td>
 +
        <td>0.112</td>
 +
        <td>0.124</td>
 +
        <td>0.114</td>
 +
        <td>0.094</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>0.115</td><br>        <td>0.112</td>
 +
        <td>0.107</td>
 +
        <td>0.108</td>
 +
        <td>0.109</td>
 +
        <td>0.109</td>
 +
        <td>0.11</td>
 +
        <td>0.111</td>
 +
        <td>0.096</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>0.114</td><br>        <td>0.111</td>
 +
        <td>0.107</td>
 +
        <td>0.107</td>
 +
        <td>0.11</td>
 +
        <td>0.111</td>
 +
        <td>0.116</td>
 +
        <td>0.111</td>
 +
        <td>0.093</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>0.115</td><br>        <td>0.113</td><br>        <td>0.109</td><br>        <td>0.11</td>
 +
        <td>0.11</td>
 +
        <td>0.109</td><br>        <td>0.11</td>
 +
        <td>0.11</td>
 +
        <td>0.095</td>
 +
    </tr>
 +
</table>
 +
 
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 6:    </th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
        <th>LB + Chlor (blank)</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td><br>        <td>0.585</td><br>        <td>0.526</td>
 +
        <td>0.49</td>
 +
        <td>0.538</td>
 +
        <td>0.594</td>
 +
        <td>0.545</td>
 +
        <td>0.558</td>
 +
        <td>0.595</td>
 +
        <td>0.096</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>0.563</td><br>        <td>0.533</td>
 +
        <td>0.483</td>
 +
        <td>0.533</td>
 +
        <td>0.582</td>
 +
        <td>0.544</td>
 +
        <td>0.541</td>
 +
        <td>0.56</td>
 +
        <td>0.093</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>0.595</td><br>        <td>0.538</td>
 +
        <td>0.498</td>
 +
        <td>0.525</td>
 +
        <td>0.585</td>
 +
        <td>0.566</td>
 +
        <td>0.54</td>
 +
        <td>0.531</td>
 +
        <td>0.094</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td><br>        <td>0.586</td>
 +
        <td>0.532</td>
 +
        <td>0.49</td>
 +
        <td>0.494</td>
 +
        <td>0.581</td>
 +
        <td>0.571</td>
 +
        <td>0.5</td>
 +
        <td>0.569</td>
 +
        <td>0.094</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>0.548</td><br>        <td>0.543</td>
 +
        <td>0.502</td>
 +
        <td>0.527</td>
 +
        <td>0.553</td>
 +
        <td>0.565</td>
 +
        <td>0.544</td>
 +
        <td>0.564</td>
 +
        <td>0.094</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>0.566</td><br>        <td>0.543</td>
 +
        <td>0.482</td>
 +
        <td>0.536</td>
 +
        <td>0.544</td>
 +
        <td>0.544</td>
 +
        <td>0.526</td>
 +
        <td>0.541</td>
 +
        <td>0.096</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>0.528</td><br>        <td>0.514</td>
 +
        <td>0.484</td>
 +
        <td>0.535</td>
 +
        <td>0.55</td>
 +
        <td>0.519</td>
 +
        <td>0.507</td>
 +
        <td>0.561</td>
 +
        <td>0.093</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>0.52</td>
 +
        <td>0.516</td>
 +
        <td>0.462</td>
 +
        <td>0.496</td>
 +
        <td>0.53</td>
 +
        <td>0.523</td>
 +
        <td>0.488</td>
 +
        <td>0.524</td>
 +
        <td>0.097</td>
 +
    </tr>
 +
</table>
 +
 
 +
 
 +
<h2 id="fluorescence-per-od">Fluorescence per OD</h2>
 +
<h3 id="um-fluorescein-od">uM Fluorescein / OD</h3>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 0:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td><br>        <td>0.066</td>
 +
        <td>0.382</td>
 +
        <td>0.154</td>
 +
        <td>0.126</td>
 +
        <td>0.015</td>
 +
        <td>0.118</td>
 +
        <td>0.108</td>
 +
        <td>0.145</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>0.137</td>
 +
        <td>0.283</td>
 +
        <td>0.135</td>
 +
        <td>0.279</td>
 +
        <td>-0.042</td>
 +
        <td>0.062</td>
 +
        <td>0.068</td>
 +
        <td>0.096</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>0.130</td>
 +
        <td>0.302</td>
 +
        <td>0.131</td>
 +
        <td>0.233</td>
 +
        <td>-0.027</td>
 +
        <td>0.072</td>
 +
        <td>0.069</td>
 +
        <td>0.101</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td><br>        <td>0.122</td><br>        <td>0.293</td>
 +
        <td>0.153</td>
 +
        <td>0.214</td>
 +
        <td>-0.018</td>
 +
        <td>0.091</td>
 +
        <td>0.063</td>
 +
        <td>0.106</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>0.100</td>
 +
        <td>0.170</td>
 +
        <td>0.115</td>
 +
        <td>0.231</td>
 +
        <td>-0.032</td>
 +
        <td>0.057</td>
 +
        <td>0.169</td>
 +
        <td>0.059</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>0.193</td>
 +
        <td>0.270</td>
 +
        <td>0.167</td>
 +
        <td>0.278</td>
 +
        <td>-0.011</td>
 +
        <td>0.083</td>
 +
        <td>0.196</td>
 +
        <td>0.114</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>0.146</td>
 +
        <td>0.229</td>
 +
        <td>0.109</td>
 +
        <td>0.221</td>
 +
        <td>-0.036</td>
 +
        <td>0.071</td>
 +
        <td>0.127</td>
 +
        <td>0.088</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>0.037</td><br>        <td>0.209</td><br>        <td>0.139</td>
 +
        <td>0.056</td><br>        <td>-0.064</td>
 +
        <td>0.046</td>
 +
        <td>0.213</td>
 +
        <td>0.050</td>
 +
    </tr>
 +
</table> 
 +
 
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 6:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td><br>        <td>0.003</td><br>        <td>0.178</td><br>        <td>0.086</td><br>        <td>0.139</td><br>        <td>0.003</td><br>        <td>0.006</td><br>        <td>0.014</td><br>        <td>0.066</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>0.005</td><br>        <td>0.167</td><br>        <td>0.083</td><br>        <td>0.145</td><br>        <td>0.005</td><br>        <td>0.009</td><br>        <td>0.014</td><br>        <td>0.069</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>0.005</td><br>        <td>0.156</td><br>        <td>0.079</td><br>        <td>0.144</td><br>        <td>0.006</td><br>        <td>0.008</td><br>        <td>0.015</td><br>        <td>0.072</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td><br>        <td>0.006</td><br>        <td>0.160</td><br>        <td>0.079</td><br>        <td>0.145</td><br>        <td>0.007</td><br>        <td>0.009</td><br>        <td>0.018</td><br>        <td>0.068</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>0.005</td><br>        <td>0.136</td><br>        <td>0.095</td><br>        <td>0.137</td><br>        <td>0.005</td><br>        <td>0.010</td><br>        <td>0.034</td><br>        <td>0.062</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>0.006</td><br>        <td>0.139</td><br>        <td>0.105</td><br>        <td>0.143</td><br>        <td>0.006</td><br>        <td>0.011</td><br>        <td>0.039</td><br>        <td>0.065</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>0.007</td><br>        <td>0.145</td><br>        <td>0.103</td><br>        <td>0.143</td><br>        <td>0.005</td><br>        <td>0.013</td><br>        <td>0.040</td><br>        <td>0.059</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>0.003</td><br>        <td>0.162</td><br>        <td>0.123</td><br>        <td>0.152</td><br>        <td>0.005</td><br>        <td>0.011</td><br>        <td>0.049</td><br>        <td>0.069</td>
 +
    </tr>
 +
</table>
 +
 
 +
 
 +
 
 +
<h3 id="net-fluorescein-a-u-">Net Fluorescein a.u.</h3>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 0:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td>
 +
        <td>11.64</td><br>        <td>59.32</td><br>        <td>23.96</td><br>        <td>24.82</td><br>        <td>2.83</td>
 +
        <td>19.53</td><br>        <td>14.52</td><br>        <td>22.51</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>22.70</td><br>        <td>43.92</td><br>        <td>15.41</td><br>        <td>31.76</td><br>        <td>-5.27</td><br>        <td>7.72</td>
 +
        <td>6.30</td>
 +
        <td>14.89</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>28.24</td>
 +
        <td>53.23</td>
 +
        <td>17.59</td>
 +
        <td>36.23</td>
 +
        <td>-4.43</td>
 +
        <td>11.21</td>
 +
        <td>9.35</td>
 +
        <td>18.81</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td>
 +
        <td>23.96</td><br>        <td>45.56</td><br>        <td>17.48</td><br>        <td>28.79</td><br>        <td>-2.66</td><br>        <td>11.28</td><br>        <td>6.56</td>
 +
        <td>15.36</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>21.81</td><br>        <td>35.19</td><br>        <td>16.70</td><br>        <td>69.53</td><br>        <td>-5.65</td><br>        <td>10.71</td><br>        <td>52.54</td><br>        <td>12.16</td><br>    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>38.05</td><br>        <td>44.72</td><br>        <td>19.04</td><br>        <td>34.55</td><br>        <td>-1.52</td><br>        <td>11.23</td><br>        <td>28.45</td><br>        <td>17.78</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>31.80</td><br>        <td>42.74</td><br>        <td>15.87</td><br>        <td>32.03</td><br>        <td>-6.41</td><br>        <td>13.28</td><br>        <td>30.29</td><br>        <td>16.36</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>7.73</td>
 +
        <td>38.93</td><br>        <td>20.15</td><br>        <td>8.66</td>
 +
        <td>-9.92</td><br>        <td>6.60</td>
 +
        <td>33.04</td><br>        <td>7.84</td>
 +
    </tr>
 +
</table>
 +
 
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 6:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td>
 +
        <td>17.37</td>
 +
        <td>793.26</td><br>        <td>351.36</td><br>        <td>638.58</td><br>        <td>15.63</td>
 +
        <td>28.08</td>
 +
        <td>66.53</td>
 +
        <td>342.12</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>26.70</td>
 +
        <td>759.50</td><br>        <td>334.93</td><br>        <td>662.65</td><br>        <td>24.67</td>
 +
        <td>41.61</td>
 +
        <td>65.90</td>
 +
        <td>333.82</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>27.84</td>
 +
        <td>715.38</td><br>        <td>329.02</td><br>        <td>643.51</td><br>        <td>29.00</td>
 +
        <td>41.07</td>
 +
        <td>70.77</td>
 +
        <td>327.73</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td>
 +
        <td>29.82</td>
 +
        <td>724.00</td>
 +
        <td>323.41</td>
 +
        <td>600.97</td><br>        <td>35.22</td>
 +
        <td>44.14</td>
 +
        <td>77.51</td>
 +
        <td>334.09</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>24.12</td>
 +
        <td>631.60</td><br>        <td>400.91</td><br>        <td>616.52</td><br>        <td>25.66</td>
 +
        <td>47.64</td>
 +
        <td>157.92</td><br>        <td>299.65</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>27.73</td>
 +
        <td>644.75</td><br>        <td>420.79</td><br>        <td>649.82</td><br>        <td>27.92</td>
 +
        <td>51.88</td>
 +
        <td>172.24</td><br>        <td>297.58</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>30.80</td>
 +
        <td>633.32</td><br>        <td>415.51</td><br>        <td>656.88</td><br>        <td>25.22</td>
 +
        <td>58.43</td>
 +
        <td>173.59</td><br>        <td>286.40</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>13.89</td>
 +
        <td>702.26</td><br>        <td>463.15</td><br>        <td>627.04</td><br>        <td>22.83</td>
 +
        <td>48.48</td>
 +
        <td>196.84</td><br>        <td>306.56</td>
 +
    </tr>
 +
</table>
 +
 
 +
<h3 id="net-abs-600">Net Abs 600</h3>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 0:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td>
 +
        <td>0.017</td><br>        <td>0.015</td><br>        <td>0.015</td><br>        <td>0.019</td><br>        <td>0.018</td><br>        <td>0.016</td><br>        <td>0.013</td><br>        <td>0.015</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>0.016</td><br>        <td>0.015</td><br>        <td>0.011</td><br>        <td>0.011</td><br>        <td>0.012</td><br>        <td>0.012</td><br>        <td>0.009</td><br>        <td>0.015</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>0.021</td><br>        <td>0.017</td><br>        <td>0.013</td><br>        <td>0.015</td><br>        <td>0.016</td><br>        <td>0.015</td><br>        <td>0.013</td><br>        <td>0.018</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td>
 +
        <td>0.019</td><br>        <td>0.015</td><br>        <td>0.011</td><br>        <td>0.013</td><br>        <td>0.014</td><br>        <td>0.012</td><br>        <td>0.010</td><br>        <td>0.014</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>0.021</td><br>        <td>0.020</td><br>        <td>0.014</td><br>        <td>0.029</td><br>        <td>0.017</td><br>        <td>0.018</td><br>        <td>0.030</td><br>        <td>0.020</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>0.019</td><br>        <td>0.016</td><br>        <td>0.011</td><br>        <td>0.012</td><br>        <td>0.013</td><br>        <td>0.013</td><br>        <td>0.014</td><br>        <td>0.015</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>0.021</td><br>        <td>0.018</td><br>        <td>0.014</td><br>        <td>0.014</td><br>        <td>0.017</td><br>        <td>0.018</td><br>        <td>0.023</td><br>        <td>0.018</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>0.020</td><br>        <td>0.018</td><br>        <td>0.014</td><br>        <td>0.015</td><br>        <td>0.015</td><br>        <td>0.014</td><br>        <td>0.015</td><br>        <td>0.015</td>
 +
    </tr>
 +
</table>
 +
 
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 6:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td>
 +
        <td>0.489</td><br>        <td>0.430</td><br>        <td>0.394</td><br>        <td>0.442</td><br>        <td>0.498</td><br>        <td>0.449</td><br>        <td>0.462</td><br>        <td>0.499</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>0.470</td><br>        <td>0.440</td><br>        <td>0.390</td><br>        <td>0.440</td><br>        <td>0.489</td><br>        <td>0.451</td><br>        <td>0.448</td><br>        <td>0.467</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>0.501</td><br>        <td>0.444</td><br>        <td>0.404</td><br>        <td>0.431</td><br>        <td>0.491</td><br>        <td>0.472</td><br>        <td>0.446</td><br>        <td>0.437</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td>
 +
        <td>0.492</td><br>        <td>0.438</td><br>        <td>0.396</td><br>        <td>0.400</td><br>        <td>0.487</td><br>        <td>0.477</td><br>        <td>0.406</td><br>        <td>0.475</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>0.454</td><br>        <td>0.449</td><br>        <td>0.408</td><br>        <td>0.433</td><br>        <td>0.459</td><br>        <td>0.471</td><br>        <td>0.450</td><br>        <td>0.470</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>0.470</td><br>        <td>0.447</td><br>        <td>0.386</td><br>        <td>0.440</td><br>        <td>0.448</td><br>        <td>0.448</td><br>        <td>0.430</td><br>        <td>0.445</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>0.435</td><br>        <td>0.421</td><br>        <td>0.391</td><br>        <td>0.442</td><br>        <td>0.457</td><br>        <td>0.426</td><br>        <td>0.414</td><br>        <td>0.468</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>0.423</td><br>        <td>0.419</td><br>        <td>0.365</td><br>        <td>0.399</td><br>        <td>0.433</td><br>        <td>0.426</td><br>        <td>0.391</td><br>        <td>0.427</td>
 +
    </tr>
 +
</table>
 +
 
 +
<h2 id="fluorescence-per-particle">Fluorescence per Particle</h2>
 +
<h3 id="mefl-particle">MEFL / particle</h3>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 0:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td>
 +
        <td>2.24E+03</td><br>        <td>1.29E+04</td><br>        <td>5.23E+03</td><br>        <td>4.27E+03</td><br>        <td>5.14E+02</td><br>        <td>3.99E+03</td><br>        <td>3.65E+03</td><br>        <td>4.91E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>4.64E+03</td><br>        <td>9.58E+03</td><br>        <td>4.58E+03</td><br>        <td>9.45E+03</td><br>        <td>-1.44E+03</td><br>        <td>2.11E+03</td><br>        <td>2.29E+03</td><br>        <td>3.25E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>4.40E+03</td>
 +
        <td>1.02E+04</td>
 +
        <td>4.43E+03</td>
 +
        <td>7.90E+03</td>
 +
        <td>-9.06E+02</td><br>        <td>2.44E+03</td>
 +
        <td>2.35E+03</td>
 +
        <td>3.42E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td>
 +
        <td>4.12E+03</td>
 +
        <td>9.94E+03</td>
 +
        <td>5.20E+03</td>
 +
        <td>7.24E+03</td>
 +
        <td>-6.22E+02</td><br>        <td>3.07E+03</td><br>        <td>2.15E+03</td>
 +
        <td>3.59E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>3.40E+03</td>
 +
        <td>5.76E+03</td>
 +
        <td>3.90E+03</td>
 +
        <td>7.84E+03</td>
 +
        <td>-1.09E+03</td><br>        <td>1.95E+03</td>
 +
        <td>5.73E+03</td>
 +
        <td>1.99E+03</td>
 +
    </tr>
 +
        <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>6.55E+03</td><br>        <td>9.14E+03</td><br>        <td>5.66E+03</td><br>        <td>9.42E+03</td><br>        <td>-3.82E+02</td><br>        <td>2.83E+03</td><br>        <td>6.65E+03</td><br>        <td>3.88E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>4.95E+03</td><br>        <td>7.77E+03</td><br>        <td>3.71E+03</td><br>        <td>7.48E+03</td><br>        <td>-1.23E+03</td><br>        <td>2.41E+03</td><br>        <td>4.31E+03</td><br>        <td>2.97E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>1.26E+03</td>
 +
        <td>7.08E+03</td>
 +
        <td>4.71E+03</td>
 +
        <td>1.89E+03</td>
 +
        <td>-2.16E+03</td><br>        <td>1.54E+03</td>
 +
        <td>7.21E+03</td>
 +
        <td>1.71E+03</td>
 +
    </tr>
 +
</table>
 +
 
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 6:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td>
 +
        <td>1.16E+02</td><br>        <td>6.03E+03</td><br>        <td>2.92E+03</td><br>        <td>4.73E+03</td><br>        <td>1.03E+02</td><br>        <td>2.05E+02</td><br>        <td>4.71E+02</td><br>        <td>2.24E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>1.86E+02</td><br>        <td>5.65E+03</td><br>        <td>2.81E+03</td><br>        <td>4.93E+03</td><br>        <td>1.65E+02</td><br>        <td>3.02E+02</td><br>        <td>4.81E+02</td><br>        <td>2.34E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>1.82E+02</td><br>        <td>5.27E+03</td><br>        <td>2.66E+03</td><br>        <td>4.88E+03</td><br>        <td>1.93E+02</td><br>        <td>2.85E+02</td><br>        <td>5.19E+02</td><br>        <td>2.45E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td>
 +
        <td>1.98E+02</td><br>        <td>5.41E+03</td><br>        <td>2.67E+03</td><br>        <td>4.91E+03</td><br>        <td>2.37E+02</td><br>        <td>3.03E+02</td><br>        <td>6.25E+02</td><br>        <td>2.30E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>1.74E+02</td><br>        <td>4.60E+03</td><br>        <td>3.21E+03</td><br>        <td>4.66E+03</td><br>        <td>1.83E+02</td><br>        <td>3.31E+02</td><br>        <td>1.15E+03</td><br>        <td>2.09E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>1.93E+02</td><br>        <td>4.72E+03</td><br>        <td>3.57E+03</td><br>        <td>4.83E+03</td><br>        <td>2.04E+02</td><br>        <td>3.79E+02</td><br>        <td>1.31E+03</td><br>        <td>2.19E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>2.32E+02</td><br>        <td>4.92E+03</td><br>        <td>3.48E+03</td><br>        <td>4.86E+03</td><br>        <td>1.81E+02</td><br>        <td>4.49E+02</td><br>        <td>1.37E+03</td><br>        <td>2.00E+03</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>1.07E+02</td><br>        <td>5.48E+03</td><br>        <td>4.15E+03</td><br>        <td>5.14E+03</td><br>        <td>1.72E+02</td><br>        <td>3.72E+02</td><br>        <td>1.65E+03</td><br>        <td>2.35E+03</td>
 +
    </tr>
 +
</table>
 +
 
 +
<h3 id="net-fluorescein-a-u-">Net Fluorescein a.u.</h3>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 0:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td>
 +
        <td>11.64</td><br>        <td>59.32</td><br>        <td>23.96</td><br>        <td>24.82</td><br>        <td>2.83</td><br>        <td>19.53</td><br>        <td>14.52</td><br>        <td>22.51</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>22.70</td><br>        <td>43.92</td><br>        <td>15.41</td><br>        <td>31.76</td><br>        <td>-5.27</td><br>        <td>7.72</td>
 +
        <td>6.30</td>
 +
        <td>14.89</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>28.24</td><br>        <td>53.23</td><br>        <td>17.59</td><br>        <td>36.23</td><br>        <td>-4.43</td><br>        <td>11.21</td><br>        <td>9.35</td>
 +
        <td>18.81</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td>
 +
        <td>23.96</td><br>        <td>45.56</td><br>        <td>17.48</td><br>        <td>28.79</td><br>        <td>-2.66</td><br>        <td>11.28</td><br>        <td>6.56</td>
 +
        <td>15.36</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>21.81</td><br>        <td>35.19</td><br>        <td>16.70</td><br>        <td>69.53</td><br>        <td>-5.65</td><br>        <td>10.71</td><br>        <td>52.54</td><br>        <td>12.16</td>
 +
    </tr>
 +
        <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>38.05</td><br>        <td>44.72</td><br>        <td>19.04</td><br>        <td>34.55</td><br>        <td>-1.52</td><br>        <td>11.23</td><br>        <td>28.45</td><br>        <td>17.78</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>31.80</td><br>        <td>42.74</td><br>        <td>15.87</td><br>        <td>32.03</td><br>        <td>-6.41</td><br>        <td>13.28</td><br>        <td>30.29</td><br>        <td>16.36</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>7.73</td><br>        <td>38.93</td><br>        <td>20.15</td><br>        <td>8.66</td><br>        <td>-9.92</td>
 +
        <td>6.60</td>
 +
        <td>33.04</td>
 +
        <td>7.84</td>
 +
    </tr>
 +
</table>
 +
 
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 6:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td>
 +
        <td>17.37</td>
 +
        <td>793.26</td><br>        <td>351.36</td><br>        <td>638.58</td><br>        <td>15.63</td>
 +
        <td>28.08</td>
 +
        <td>66.53</td>
 +
        <td>342.12</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>26.70</td>
 +
        <td>759.50</td><br>        <td>334.93</td><br>        <td>662.65</td><br>        <td>24.67</td>
 +
        <td>41.61</td>
 +
        <td>65.90</td>
 +
        <td>333.82</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>27.84</td>
 +
        <td>715.38</td><br>        <td>329.02</td><br>        <td>643.51</td><br>        <td>29.00</td>
 +
        <td>41.07</td>
 +
        <td>70.77</td>
 +
        <td>327.73</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td>
 +
        <td>29.82</td>
 +
        <td>724.00</td><br>        <td>323.41</td><br>        <td>600.97</td><br>        <td>35.22</td>
 +
        <td>44.14</td>
 +
        <td>77.51</td>
 +
        <td>334.09</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>24.12</td>
 +
        <td>631.60</td><br>        <td>400.91</td><br>        <td>616.52</td><br>        <td>25.66</td>
 +
        <td>47.64</td>
 +
        <td>157.92</td>
 +
        <td>299.65</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>27.73</td>
 +
        <td>644.75</td><br>        <td>420.79</td><br>        <td>649.82</td><br>        <td>27.92</td>
 +
        <td>51.88</td>
 +
        <td>172.24</td><br>        <td>297.58</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>30.80</td>
 +
        <td>633.32</td><br>        <td>415.51</td><br>        <td>656.88</td><br>        <td>25.22</td>
 +
        <td>58.43</td>
 +
        <td>173.59</td><br>        <td>286.40</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>13.89</td>
 +
        <td>702.26</td><br>        <td>463.15</td><br>        <td>627.04</td><br>        <td>22.83</td>
 +
        <td>48.48</td>
 +
        <td>196.84</td><br>        <td>306.56</td>
 +
    </tr>
 +
</table>
 +
 
 +
<h3 id="net-abs-600">Net Abs 600</h3>
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 0:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td>
 +
        <td>0.017</td><br>        <td>0.015</td><br>        <td>0.015</td><br>        <td>0.019</td><br>        <td>0.018</td><br>        <td>0.016</td><br>        <td>0.013</td><br>        <td>0.015</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>0.016</td><br>        <td>0.015</td><br>        <td>0.011</td><br>        <td>0.011</td><br>        <td>0.012</td><br>        <td>0.012</td><br>        <td>0.009</td><br>        <td>0.015</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>0.021</td><br>        <td>0.017</td><br>        <td>0.013</td><br>        <td>0.015</td><br>        <td>0.016</td><br>        <td>0.015</td><br>        <td>0.013</td><br>        <td>0.018</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td>
 +
        <td>0.019</td><br>        <td>0.015</td><br>        <td>0.011</td><br>        <td>0.013</td><br>        <td>0.014</td><br>        <td>0.012</td><br>        <td>0.010</td><br>        <td>0.014</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>0.021</td><br>        <td>0.020</td><br>        <td>0.014</td><br>        <td>0.029</td><br>        <td>0.017</td><br>        <td>0.018</td><br>        <td>0.030</td><br>        <td>0.020</td>
 +
    </tr>
 +
        <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>0.019</td><br>        <td>0.016</td><br>        <td>0.011</td><br>        <td>0.012</td><br>        <td>0.013</td><br>        <td>0.013</td><br>        <td>0.014</td><br>        <td>0.015</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>0.021</td><br>        <td>0.018</td><br>        <td>0.014</td><br>        <td>0.014</td><br>        <td>0.017</td><br>        <td>0.018</td><br>        <td>0.023</td><br>        <td>0.018</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>0.020</td><br>        <td>0.018</td><br>        <td>0.014</td><br>        <td>0.015</td><br>        <td>0.015</td><br>        <td>0.014</td><br>        <td>0.015</td><br>        <td>0.015</td>
 +
    </tr>
 +
</table>
 +
 
 +
<table style="width:100%" id="parts">
 +
    <tr>
 +
        <th>Hour 6:</th>
 +
        <th>Neg. Control</th>
 +
        <th>Pos. Control</th>
 +
        <th>Device 1</th>
 +
        <th>Device 2</th>
 +
        <th>Device 3</th>
 +
        <th>Device 4</th>
 +
        <th>Device 5</th>
 +
        <th>Device 6</th>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 1</td>
 +
        <td>0.489</td><br>        <td>0.430</td><br>        <td>0.394</td><br>        <td>0.442</td><br>        <td>0.498</td><br>        <td>0.449</td><br>        <td>0.462</td><br>        <td>0.499</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 2</td>
 +
        <td>0.470</td><br>        <td>0.440</td><br>        <td>0.390</td><br>        <td>0.440</td><br>        <td>0.489</td><br>        <td>0.451</td><br>        <td>0.448</td><br>        <td>0.467</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 3</td>
 +
        <td>0.501</td><br>        <td>0.444</td><br>        <td>0.404</td><br>        <td>0.431</td><br>        <td>0.491</td><br>        <td>0.472</td><br>        <td>0.446</td><br>        <td>0.437</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 1, Replicate 4</td>
 +
        <td>0.492</td><br>        <td>0.438</td><br>        <td>0.396</td><br>        <td>0.400</td><br>        <td>0.487</td><br>        <td>0.477</td><br>        <td>0.406</td><br>        <td>0.475</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 1</td>
 +
        <td>0.454</td><br>        <td>0.449</td><br>        <td>0.408</td><br>        <td>0.433</td><br>        <td>0.459</td><br>        <td>0.471</td><br>        <td>0.450</td><br>        <td>0.470</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 2</td>
 +
        <td>0.470</td><br>        <td>0.447</td><br>        <td>0.386</td><br>        <td>0.440</td><br>        <td>0.448</td><br>        <td>0.448</td><br>        <td>0.430</td><br>        <td>0.445</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 3</td>
 +
        <td>0.435</td><br>        <td>0.421</td><br>        <td>0.391</td><br>        <td>0.442</td><br>        <td>0.457</td><br>        <td>0.426</td><br>        <td>0.414</td><br>        <td>0.468</td>
 +
    </tr>
 +
    <tr>
 +
        <td>Colony 2, Replicate 4</td>
 +
        <td>0.423</td><br>        <td>0.419</td><br>        <td>0.365</td><br>        <td>0.399</td><br>        <td>0.433</td><br>        <td>0.426</td><br>        <td>0.391</td><br>        <td>0.427</td>
 +
    </tr>
 +
</table>

Revision as of 20:15, 6 October 2018

InterLab

We did this year's iGEM InterLab study!
Our original Excel file can be downloaded here.
Here is the protocol that we followed.

OD600 Reference Point - LUDOX Protocol

LUDOX CL-X H2O
Replicate 1 0.113 0.087
Replicate 2 0.105 0.085
Replicate 3 0.119 0.084
Replicate 4 0.103 0.085
Arith. Mean 0.110 0.085
Corrected Abs600 0.025
Reference OD600 0.063
OD600/Abs600 2.545

Particle Standard Curve: Microsphere Protocol

Need to color code but: Abs600 measurements are blue cells. Gold cells are calculated.






Number of Particles 2.35E+08 1.18E+08 5.88E+07 2.94E+07 1.47E+07 7.35E+06 3.68E+06 1.84E+06 9.19E+05 4.60E+05 2.30E+05 0
Replicate 1 0.534 0.337 0.211 0.148 0.124 0.102 0.094 0.091 0.089 0.089 0.088 0.087
Replicate 2 0.538 0.329 0.204 0.145 0.117 0.102 0.099 0.092 0.089 0.089 0.086 0.085
Replicate 3 0.543 0.332 0.209 0.145 0.116 0.107 0.093 0.090 0.088 0.089 0.086 0.083
Replicate 4 0.545 0.314 0.208 0.143 0.119 0.102 0.092 0.089 0.089 0.089 0.086 0.083
Arith. Mean 0.540 0.328 0.208 0.145 0.119 0.103 0.095 0.091 0.089 0.089 0.087 0.085
Arith. Std.Dev. 0.005 0.010 0.003 0.002 0.004 0.003 0.003 0.001 0.001 0.000 0.001 0.002
Arith. Net Mean 0.456 0.244 0.124 0.061 0.035 0.019 0.010 0.006 0.004 0.004 0.002

<img src="T--Virginia--2018_ParticleStandardCurve.png" alt="ParticleStandardCurve"> <img src="T--Virginia--2018_ParticleStandardCurve_log.png" alt="ParticleStandardCurve_log">

Particles/OD

Note: Final scaling level determined from medium-high points likely to be less impacted by saturation or pipetting error. If needed, you can shift which points are used, but it is likely better to correct instrument settings and protocol.


Number of Particles 2.35E+08 1.18E+08 5.88E+07 2.94E+07 1.47E+07 7.35E+06 3.68E+06 1.84E+06 9.19E+05 4.60E+05 2.30E+05
Mean particles / Abs600 5.17E+08 4.83E+08 4.76E+08 4.84E+08 4.26E+08 3.92E+08 3.68E+08 3.06E+08 2.16E+08 1.02E+08 1.15E+08
Mean of med-high levels: 4.52E+08

Cospheric Monodisperse Silica Microspheres 0.961um diameter


Spheres/gram Grams/mL Spheres/0.55mL Resuspend volume mL: Dilution X: Total volume mL: Particles/mL: Well volume (mL) Initial particles:
1.20E+12 1.8 1.19E+12 2.55 100 255 4.71E+09 0.1 4.71E+08


Raw Plate Reader Measurements

Fluorescence Raw Readings:












Hour 0: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)
Colony 1, Replicate 1 124.47 172.15 136.79 137.65 115.66 132.36 127.35 135.34 112.83
Colony 1, Replicate 2 121.01 142.23 113.72 130.07 93.041 106.03 104.61 113.2 98.307
Colony 1, Replicate 3 123.38 148.37 112.73 131.37 90.708 106.35 104.49 113.95 95.141
Colony 1, Replicate 4 119.97 141.57 113.49 124.8 93.35 107.29 102.57 111.37 96.012
Colony 2, Replicate 1 121.32 134.7 116.21 169.04 93.865 110.22 152.05 111.67 99.513
Colony 2, Replicate 2 134.51 141.18 115.5 131.01 94.94 107.69 124.91 114.24 96.457
Colony 2, Replicate 3 130.47 141.41 114.54 130.7 92.262 111.95 128.96 115.03 98.671
Colony 2, Replicate 4 129.77 160.97 142.19 130.7 112.12 128.64 155.08 129.88 122.04









Hour 6: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)
Colony 1, Replicate 1 147.58 923.47 481.57 768.79 145.84 158.29 196.74 472.33 130.21
Colony 1, Replicate 2 135.57 868.37 443.8 771.52 133.54 150.48 174.77 442.69 108.87
Colony 1, Replicate 3 133.91 821.45 435.09 749.58 135.07 147.14 176.84 433.8 106.07
Colony 1, Replicate 4 131.16 825.34 424.75 702.31 136.56 145.48 178.85 435.43 101.34
Colony 2, Replicate 1 129.25 736.73 506.04 721.65 130.79 152.77 263.05 404.78 105.13
Colony 2, Replicate 2 129.93 746.95 522.99 752.02 130.12 154.08 274.44 99.78 102.2
Colony 2, Replicate 3 134.9 737.42 519.61 760.98 129.32 162.53 277.69 390.5 104.1
Colony 2, Replicate 4 136.44 824.81 585.7 749.59 145.38 171.03 319.39 429.11 122.55

Abs600 Raw Readings















Hour 0: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)
Colony 1, Replicate 1 0.114 0.112 0.112 0.116 0.115 0.113 0.11 0.112 0.097
Colony 1, Replicate 2 0.113 0.112 0.108 0.108 0.109 0.109 0.106 0.112 0.097
Colony 1, Replicate 3 0.114 0.11 0.106 0.108 0.109 0.108 0.106 0.111 0.093
Colony 1, Replicate 4 0.114 0.11 0.106 0.108 0.109 0.107 0.105 0.109 0.095
Colony 2, Replicate 1 0.115 0.114 0.108 0.123 0.111 0.112 0.124 0.114 0.094
Colony 2, Replicate 2 0.115 0.112 0.107 0.108 0.109 0.109 0.11 0.111 0.096
Colony 2, Replicate 3 0.114 0.111 0.107 0.107 0.11 0.111 0.116 0.111 0.093
Colony 2, Replicate 4 0.115 0.113 0.109 0.11 0.11 0.109 0.11 0.11 0.095








Hour 6: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)
Colony 1, Replicate 1 0.585 0.526 0.49 0.538 0.594 0.545 0.558 0.595 0.096
Colony 1, Replicate 2 0.563 0.533 0.483 0.533 0.582 0.544 0.541 0.56 0.093
Colony 1, Replicate 3 0.595 0.538 0.498 0.525 0.585 0.566 0.54 0.531 0.094
Colony 1, Replicate 4 0.586 0.532 0.49 0.494 0.581 0.571 0.5 0.569 0.094
Colony 2, Replicate 1 0.548 0.543 0.502 0.527 0.553 0.565 0.544 0.564 0.094
Colony 2, Replicate 2 0.566 0.543 0.482 0.536 0.544 0.544 0.526 0.541 0.096
Colony 2, Replicate 3 0.528 0.514 0.484 0.535 0.55 0.519 0.507 0.561 0.093
Colony 2, Replicate 4 0.52 0.516 0.462 0.496 0.53 0.523 0.488 0.524 0.097


Fluorescence per OD

uM Fluorescein / OD







Hour 0: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 0.066 0.382 0.154 0.126 0.015 0.118 0.108 0.145
Colony 1, Replicate 2 0.137 0.283 0.135 0.279 -0.042 0.062 0.068 0.096
Colony 1, Replicate 3 0.130 0.302 0.131 0.233 -0.027 0.072 0.069 0.101
Colony 1, Replicate 4 0.122 0.293 0.153 0.214 -0.018 0.091 0.063 0.106
Colony 2, Replicate 1 0.100 0.170 0.115 0.231 -0.032 0.057 0.169 0.059
Colony 2, Replicate 2 0.193 0.270 0.167 0.278 -0.011 0.083 0.196 0.114
Colony 2, Replicate 3 0.146 0.229 0.109 0.221 -0.036 0.071 0.127 0.088
Colony 2, Replicate 4 0.037 0.209 0.139 0.056 -0.064 0.046 0.213 0.050


























































Hour 6: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 0.003 0.178 0.086 0.139 0.003 0.006 0.014 0.066
Colony 1, Replicate 2 0.005 0.167 0.083 0.145 0.005 0.009 0.014 0.069
Colony 1, Replicate 3 0.005 0.156 0.079 0.144 0.006 0.008 0.015 0.072
Colony 1, Replicate 4 0.006 0.160 0.079 0.145 0.007 0.009 0.018 0.068
Colony 2, Replicate 1 0.005 0.136 0.095 0.137 0.005 0.010 0.034 0.062
Colony 2, Replicate 2 0.006 0.139 0.105 0.143 0.006 0.011 0.039 0.065
Colony 2, Replicate 3 0.007 0.145 0.103 0.143 0.005 0.013 0.040 0.059
Colony 2, Replicate 4 0.003 0.162 0.123 0.152 0.005 0.011 0.049 0.069


Net Fluorescein a.u.












































Hour 0: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 11.64 59.32 23.96 24.82 2.83 19.53 14.52 22.51
Colony 1, Replicate 2 22.70 43.92 15.41 31.76 -5.27 7.72 6.30 14.89
Colony 1, Replicate 3 28.24 53.23 17.59 36.23 -4.43 11.21 9.35 18.81
Colony 1, Replicate 4 23.96 45.56 17.48 28.79 -2.66 11.28 6.56 15.36
Colony 2, Replicate 1 21.81 35.19 16.70 69.53 -5.65 10.71 52.54 12.16
Colony 2, Replicate 2 38.05 44.72 19.04 34.55 -1.52 11.23 28.45 17.78
Colony 2, Replicate 3 31.80 42.74 15.87 32.03 -6.41 13.28 30.29 16.36
Colony 2, Replicate 4 7.73 38.93 20.15 8.66 -9.92 6.60 33.04 7.84


























Hour 6: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 17.37 793.26 351.36 638.58 15.63 28.08 66.53 342.12
Colony 1, Replicate 2 26.70 759.50 334.93 662.65 24.67 41.61 65.90 333.82
Colony 1, Replicate 3 27.84 715.38 329.02 643.51 29.00 41.07 70.77 327.73
Colony 1, Replicate 4 29.82 724.00 323.41 600.97 35.22 44.14 77.51 334.09
Colony 2, Replicate 1 24.12 631.60 400.91 616.52 25.66 47.64 157.92 299.65
Colony 2, Replicate 2 27.73 644.75 420.79 649.82 27.92 51.88 172.24 297.58
Colony 2, Replicate 3 30.80 633.32 415.51 656.88 25.22 58.43 173.59 286.40
Colony 2, Replicate 4 13.89 702.26 463.15 627.04 22.83 48.48 196.84 306.56

Net Abs 600

























































Hour 0: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 0.017 0.015 0.015 0.019 0.018 0.016 0.013 0.015
Colony 1, Replicate 2 0.016 0.015 0.011 0.011 0.012 0.012 0.009 0.015
Colony 1, Replicate 3 0.021 0.017 0.013 0.015 0.016 0.015 0.013 0.018
Colony 1, Replicate 4 0.019 0.015 0.011 0.013 0.014 0.012 0.010 0.014
Colony 2, Replicate 1 0.021 0.020 0.014 0.029 0.017 0.018 0.030 0.020
Colony 2, Replicate 2 0.019 0.016 0.011 0.012 0.013 0.013 0.014 0.015
Colony 2, Replicate 3 0.021 0.018 0.014 0.014 0.017 0.018 0.023 0.018
Colony 2, Replicate 4 0.020 0.018 0.014 0.015 0.015 0.014 0.015 0.015
























































Hour 6: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 0.489 0.430 0.394 0.442 0.498 0.449 0.462 0.499
Colony 1, Replicate 2 0.470 0.440 0.390 0.440 0.489 0.451 0.448 0.467
Colony 1, Replicate 3 0.501 0.444 0.404 0.431 0.491 0.472 0.446 0.437
Colony 1, Replicate 4 0.492 0.438 0.396 0.400 0.487 0.477 0.406 0.475
Colony 2, Replicate 1 0.454 0.449 0.408 0.433 0.459 0.471 0.450 0.470
Colony 2, Replicate 2 0.470 0.447 0.386 0.440 0.448 0.448 0.430 0.445
Colony 2, Replicate 3 0.435 0.421 0.391 0.442 0.457 0.426 0.414 0.468
Colony 2, Replicate 4 0.423 0.419 0.365 0.399 0.433 0.426 0.391 0.427

Fluorescence per Particle

MEFL / particle


































Hour 0: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 2.24E+03 1.29E+04 5.23E+03 4.27E+03 5.14E+02 3.99E+03 3.65E+03 4.91E+03
Colony 1, Replicate 2 4.64E+03 9.58E+03 4.58E+03 9.45E+03 -1.44E+03 2.11E+03 2.29E+03 3.25E+03
Colony 1, Replicate 3 4.40E+03 1.02E+04 4.43E+03 7.90E+03 -9.06E+02 2.44E+03 2.35E+03 3.42E+03
Colony 1, Replicate 4 4.12E+03 9.94E+03 5.20E+03 7.24E+03 -6.22E+02 3.07E+03 2.15E+03 3.59E+03
Colony 2, Replicate 1 3.40E+03 5.76E+03 3.90E+03 7.84E+03 -1.09E+03 1.95E+03 5.73E+03 1.99E+03
Colony 2, Replicate 2 6.55E+03 9.14E+03 5.66E+03 9.42E+03 -3.82E+02 2.83E+03 6.65E+03 3.88E+03
Colony 2, Replicate 3 4.95E+03 7.77E+03 3.71E+03 7.48E+03 -1.23E+03 2.41E+03 4.31E+03 2.97E+03
Colony 2, Replicate 4 1.26E+03 7.08E+03 4.71E+03 1.89E+03 -2.16E+03 1.54E+03 7.21E+03 1.71E+03
























































Hour 6: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 1.16E+02 6.03E+03 2.92E+03 4.73E+03 1.03E+02 2.05E+02 4.71E+02 2.24E+03
Colony 1, Replicate 2 1.86E+02 5.65E+03 2.81E+03 4.93E+03 1.65E+02 3.02E+02 4.81E+02 2.34E+03
Colony 1, Replicate 3 1.82E+02 5.27E+03 2.66E+03 4.88E+03 1.93E+02 2.85E+02 5.19E+02 2.45E+03
Colony 1, Replicate 4 1.98E+02 5.41E+03 2.67E+03 4.91E+03 2.37E+02 3.03E+02 6.25E+02 2.30E+03
Colony 2, Replicate 1 1.74E+02 4.60E+03 3.21E+03 4.66E+03 1.83E+02 3.31E+02 1.15E+03 2.09E+03
Colony 2, Replicate 2 1.93E+02 4.72E+03 3.57E+03 4.83E+03 2.04E+02 3.79E+02 1.31E+03 2.19E+03
Colony 2, Replicate 3 2.32E+02 4.92E+03 3.48E+03 4.86E+03 1.81E+02 4.49E+02 1.37E+03 2.00E+03
Colony 2, Replicate 4 1.07E+02 5.48E+03 4.15E+03 5.14E+03 1.72E+02 3.72E+02 1.65E+03 2.35E+03

Net Fluorescein a.u.


















































Hour 0: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 11.64 59.32 23.96 24.82 2.83 19.53 14.52 22.51
Colony 1, Replicate 2 22.70 43.92 15.41 31.76 -5.27 7.72 6.30 14.89
Colony 1, Replicate 3 28.24 53.23 17.59 36.23 -4.43 11.21 9.35 18.81
Colony 1, Replicate 4 23.96 45.56 17.48 28.79 -2.66 11.28 6.56 15.36
Colony 2, Replicate 1 21.81 35.19 16.70 69.53 -5.65 10.71 52.54 12.16
Colony 2, Replicate 2 38.05 44.72 19.04 34.55 -1.52 11.23 28.45 17.78
Colony 2, Replicate 3 31.80 42.74 15.87 32.03 -6.41 13.28 30.29 16.36
Colony 2, Replicate 4 7.73 38.93 20.15 8.66 -9.92 6.60 33.04 7.84



























Hour 6: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 17.37 793.26 351.36 638.58 15.63 28.08 66.53 342.12
Colony 1, Replicate 2 26.70 759.50 334.93 662.65 24.67 41.61 65.90 333.82
Colony 1, Replicate 3 27.84 715.38 329.02 643.51 29.00 41.07 70.77 327.73
Colony 1, Replicate 4 29.82 724.00 323.41 600.97 35.22 44.14 77.51 334.09
Colony 2, Replicate 1 24.12 631.60 400.91 616.52 25.66 47.64 157.92 299.65
Colony 2, Replicate 2 27.73 644.75 420.79 649.82 27.92 51.88 172.24 297.58
Colony 2, Replicate 3 30.80 633.32 415.51 656.88 25.22 58.43 173.59 286.40
Colony 2, Replicate 4 13.89 702.26 463.15 627.04 22.83 48.48 196.84 306.56

Net Abs 600

























































Hour 0: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 0.017 0.015 0.015 0.019 0.018 0.016 0.013 0.015
Colony 1, Replicate 2 0.016 0.015 0.011 0.011 0.012 0.012 0.009 0.015
Colony 1, Replicate 3 0.021 0.017 0.013 0.015 0.016 0.015 0.013 0.018
Colony 1, Replicate 4 0.019 0.015 0.011 0.013 0.014 0.012 0.010 0.014
Colony 2, Replicate 1 0.021 0.020 0.014 0.029 0.017 0.018 0.030 0.020
Colony 2, Replicate 2 0.019 0.016 0.011 0.012 0.013 0.013 0.014 0.015
Colony 2, Replicate 3 0.021 0.018 0.014 0.014 0.017 0.018 0.023 0.018
Colony 2, Replicate 4 0.020 0.018 0.014 0.015 0.015 0.014 0.015 0.015
























































Hour 6: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6
Colony 1, Replicate 1 0.489 0.430 0.394 0.442 0.498 0.449 0.462 0.499
Colony 1, Replicate 2 0.470 0.440 0.390 0.440 0.489 0.451 0.448 0.467
Colony 1, Replicate 3 0.501 0.444 0.404 0.431 0.491 0.472 0.446 0.437
Colony 1, Replicate 4 0.492 0.438 0.396 0.400 0.487 0.477 0.406 0.475
Colony 2, Replicate 1 0.454 0.449 0.408 0.433 0.459 0.471 0.450 0.470
Colony 2, Replicate 2 0.470 0.447 0.386 0.440 0.448 0.448 0.430 0.445
Colony 2, Replicate 3 0.435 0.421 0.391 0.442 0.457 0.426 0.414 0.468
Colony 2, Replicate 4 0.423 0.419 0.365 0.399 0.433 0.426 0.391 0.427