Difference between revisions of "Team:iTesla-SoundBio/description"

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{{iTesla-SoundBio}}
 
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<center>
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<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.3.1/jquery.min.js"></script>
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<h1>Build the change<br>
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<script>
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<script>
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$(document).ready(function(){
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    $("#title").text("Project Description")
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  // Add smooth scrolling to all links
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  $("a").on('click', function(event) {
  
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    // Make sure this.hash has a value before overriding default behavior
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<br>You want to see in the world</h1>
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    if (this.hash !== "") {
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</center>
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      event.preventDefault();
  
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<style>
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h1 {
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      // Using jQuery's animate() method to add smooth page scroll
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    font: 600 1.5em/1 'Raleway', sans-serif;
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    color: rgba(0,0,0,.5);
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      $('html, body').animate({
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      }, 800, function(){
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        // Add hash (#) to URL when done scrolling (default click behavior)
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span, span:after {
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body {
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}
 
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<body>
 
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<img src="https://static.igem.org/mediawiki/2018/9/9b/T--iTesla-Soundbio--Project_Description1.png" alt="DID YOU KNOW? Every year, hundreds of thousands of horseshoe crabs are caught each year and drained of up to to 30% of their blood. Why? To collect LAL, a chemical that is crucial in the detection of endotoxins in everything from drugs to medical equipment", width=65%>
 
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    padding: 30px
 
}
 
}
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@media screen and (max-height: 450px) {
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    .sidenav {padding-top: 15px;}
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    .sidenav a {font-size: 18px;}
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}
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</style>
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}
 
 
<div class="sidenav">
 
<div class="sidenav">
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<a href="#graphicsum">Graphic Summary</a>
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<a href="https://2018.igem.org/Team:iTesla-SoundBio">Main Page</a>
 
<br>
 
<br>
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<a href="#constructdesign">Construct Design</a>
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<a href=#top>Top</a>
 
<br>
 
<br>
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<a href="#Biobrick">Biobrick: <br>Factor C gene in E. coli pSB1C3</a>
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<a href=#Description><img src="https://static.igem.org/mediawiki/2018/3/3c/T--iTesla-SoundBio--ProjectDescription.png" alt="Description" style="width:100%"></a>
 
<br>
 
<br>
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<a href="#Integration">Integration: <br>Factor C in B.Subtilis Chromosomal DNA</a>
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<a href=#What Are Horseshoe Crabs><img src="https://static.igem.org/mediawiki/2018/2/24/T--iTesla-SoundBio--WhatAreThey.png" alt="Education" style="width:100%"></a>
 
<br>
 
<br>
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<a href="#Expression">Expression: <br>Factor C protein verification</a>
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<a href=#Why Are Horseshoe Crabs Bled><img src="https://static.igem.org/mediawiki/2018/2/2d/T--iTesla-SoundBio--WhyBled.png" alt="Design" style="width:100%"></a>
 
<br>
 
<br>
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<a href=#What Is LAL and Factor C><img src="https://static.igem.org/mediawiki/2018/9/9e/T--iTesla-SoundBio--LAL.png" alt="Design" style="width:100%"></a>
 
</div>
 
</div>
  
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<div class="horizontalline">
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<script>
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    $("#title").text("Human Practices")
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</script>
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<div class="main">
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<div id="top"></div>
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<div id="Research">
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<h1><strong>Research</strong></h1>
 
</div>
 
</div>
  
  
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<div id="Education">
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<div id="constructdesign">
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<h1><strong>Education</strong></h1>
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<p class="text">
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Construct design: <br>
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The goal for our project is to insert Factor C gene into the chromosomal DNA of B. Subtilis, and have it express this Factor C protein. At the same time, to fulfill iGEM competition requirements, we also need to insert Factor C gene into E.coli plasmid pSB1C3 and submit it as a biobrick for iGEM competition.
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<br>
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We decided to insert Factor C gene into E.coli plasmid pSB1C3 first; then, integrate Factor C gene into the chromosomal DNA of B. Subtilis using pAX01 integration factor (a B. Subtilis specific plasmid).
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</P>
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<div id="Design">
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<div class="horizontalline">
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<h1><strong>Design</strong></h1>
 
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<div id="Biobrick">
 
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<center><img src="https://static.igem.org/mediawiki/2018/c/c6/T--itesla-soundbio--twofragments.png", width=80%></center>
 
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<p class="text">
 
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Biobrick: <br>
 
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Factor C in E.coli pSB1C3
 
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We couldn't order Factor C from factory because it is too large (about 3000 base pairs https://benchling.com/itesla-soundbio/f/gPUeHZXf-drafts/seq-Jq87odxl-psb1c3factor-c/edit), so we order it as two fragments, FC1 and FC2.
 
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First, insert FC1 into pSB1C3. Then, insert FC2 into the plasmid, too. This pSB1C3 with full Factor C will be submitted for biobrick.
 
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</p>
 
 
</div>
 
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<div class="horizontalline">
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<div id="Integration">
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<p class="text">
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Integration: <br>
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Factor C in B. Subtilis Chromosomal DNA.
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We then digest full Factor C from Biobrick, and insert it into pAX01, a B. Subtilis integration factor. Then, we transform B. Subtilis with pAX01. After plasmid pAX01 enters B. Subtilis, there is a chance that it will integrate into chromosomal DNA.
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</p>
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<div class="horizontalline">
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<div id="expression">
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<p class="text">
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Expression
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Factor C protein extract: <br>
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B. Subtilis are used to express Factor C because they are gram positive and will not damage the Factor C protein. In contrast, E. coli is gram negative and will cleave FC protein. After B. Subtilis express and produce Factor C, we can then extract Factor C protein for further experiment.   
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Revision as of 01:05, 7 October 2018

Safety Protocols
InterLab
Team Members Attributions
Human Practices Collaborations
Project Description Design Results Biobrick Parts Lab Notebook
iT-SB 2018 iT-SB 2017

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Description
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