Difference between revisions of "Team:SMMU-China/Composite Part"

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<h2 style="text-align: center">Composite part</h2>
 
<h2 style="text-align: center">Composite part</h2>
 
</header>
 
</header>
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<p style="text-align: center">We listed other composite part we constructed this year below.</p>
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    <h2 class="inner-h">Favorite composite part</h2>
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<table>
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                                <h3 class="inner-h">BNP-AR185 T2A eGFP-polyA</h3>
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<tr>
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<p class="inner-text">For this year’s iGEM competition, our favorite composite part is BNP-AR185-polyA. This part is composed of BNP promotor, AR185 linked with eGFP by T2A and poly A.
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<td width="300px">BNP-AR185-eGFP-PloyA</td>
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                            </p>
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<td width="250px"><a href="http://parts.igem.org/Part:BBa_K2865009" target="_blank">BBa_K2865009</a></td>
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<div style="text-align:center" class="resultimage">
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<td width="100px"></td>
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                                <img src="https://static.igem.org/mediawiki/2018/f/f6/T--SMMU-China--test_image.jpg" style="width: 60%;">
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</tr>
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                            </div>
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<tr>
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<h3 class="inner-h">How BNP-AR185-polyA works</h3>
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<td>CMV-AR185-eGFP-PloyA</td>
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<p class="inner-text">As has shown presiously, BNP promoter activity is related to the severity of heart failure. So we want to use ET-1, one stimulus factor, to imitate the environment of heart failure and induce BNP promoter expression. Then, the later sequence of the part, AR185, also can be expressed and fuction what it could  specifically bind to RyR2 in rat cardiomyocytes and have the ability to inhibit PKA dependent S2808 phosphorylation in vitro.
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<td><a href="http://parts.igem.org/Part:BBa_K2865010" target="_blank">BBa_K2865010</a></td>
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                            </p>
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<td></td>
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                            <div style="text-align:center" class="resultimage">
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                                <img src="https://static.igem.org/mediawiki/2018/f/f6/T--SMMU-China--test_image.jpg" style="width: 60%;">
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<tr>
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                            </div>
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<td>BNP-eGFP-PloyA</td>
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                                <h2 class="inner-h">See More</h2>
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<td><a href="http://parts.igem.org/Part:BBa_K2865008" target="_blank">BBa_K2865008</a></td>
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<p class="inner-text">Integrated information about AR185 and BNP promotor posted on Demonstrate page. Or you can alternatively check the<a href="http://parts.igem.org/Part:BBa_K2865009" target="_blank"><b>(BBa_K2865009)</b></a> on the Registry of Standard Biological Parts website. </p>
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<td></td>
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                                <h3 class="inner-h">We listed other composite part we constructed this year below.</h3>
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        <center>
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<td>CMV-eGFP-PloyA</td>
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<tr style="border-top: 1px #5d5d5d solid;border-bottom: 1px #5d5d5d solid">
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<td><a href="http://parts.igem.org/Part:BBa_K2865006" target="_blank">BBa_K2865006</a></td>
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<td>Name</td>
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<td></td>
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<td>Type</td>
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</tr>
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<td>Description</td>
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<td>Designer</td>
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<td>L ITR-BNP-AR185-eGFP-PloyA-R ITR</td>
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<td>Length</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2865012" target="_blank">BBa_K2865012</a></td>
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</tr>
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<td></td>
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<tr>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2865006" target="_Blank">BBa_K2865006</a></td>
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                                <tr>
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<td>Composite</td>
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<td>L ITR-CMV-AR185-eGFP-PloyA-R ITR</td>
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<td>CMV promoter-eGFP-poly(A)</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2865013" target="_blank">BBa_K2865013</a></td>
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<td>Hu Miao</td>
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<td></td>
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<td></td>
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</tr>
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</tr>
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<tr>
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<tr>
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<td>L ITR-CMV-eGFP-Poly(A)-R ITR</td>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2865008" target="_Blank">BBa_K2865008</a></td>
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<td><a href="http://parts.igem.org/BBa_K2865014" target="_blank">BBa_K2865014</a></td>
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<td>Composite</td>
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<td></td>
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<td>BNP promoter-eGFP-poly(A)</td>
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</tr> <tr>
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<td>Hu Miao</td>
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<td>L ITR-CMV-AR185-eGFP-PloyA-R ITR</td>
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<td></td>
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<td><a href="http://parts.igem.org/Part:BBa_K2865015" target="_blank">BBa_K2865015</a></td>
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</tr>
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<td></td>
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<tr>
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</tr>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2865009" target="_Blank">BBa_K2865009</a></td>
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<td>Composite</td>
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</table>
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<td>BNP-AR185-Poly(A)</td>
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<div style="text-align: center" class="resultimage">
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<td>Hu Miao</td>
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      <p style="font-style: italic"></p>
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<td></td>
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</div>
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</tr>
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<section class="paragragh" id="1st">
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<tr>
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<header>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2865010" target="_Blank">BBa_K2865010</a></td>
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<h2>Favorite composite part </h2>
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<td>Composite</td>
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</header>
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<td>CMV-AR185-Poly(A)</td>
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<header>
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<td>Hu Miao</td>
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                                                                                <h3>BNP-AR185 T2A eGFP-polyA</h3>
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<td></td>
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</header>
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</tr>
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<p>For this year’s iGEM competition, our favorite composite part is BNP-AR185-polyA. This part is composed of BNP promotor, AR185 linked with eGFP by T2A and poly A.</p>
+
<tr>
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<h3>How BNP-AR185-polyA works</h3>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2865012" target="_Blank">BBa_K2865012</a></td>
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<p>
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<td>Composite</td>
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As has shown presiously, BNP promoter activity is related to the severity of heart failure. So we want to use ET-1, one stimulus factor, to imitate the environment of heart failure and induce BNP promoter expression. Then, the later sequence of the part, AR185, also can be expressed and fuction what it could  specifically bind to RyR2 in rat cardiomyocytes and have the ability to inhibit PKA dependent S2808 phosphorylation in vitro.
+
<td>Left ITR-BNP-AR185-Poly(A)-Right ITR</td>
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</p>
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<td>Hu Miao</td>
−
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<td></td>
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<div style="text-align: center" class="resultimage">
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</tr>
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<img src="https://static.igem.org/mediawiki/2018/f/f6/T--SMMU-China--test_image.jpg">
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<tr>
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<p style="font-style: italic">Fig.1 balablablabla</p>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2865013" target="_Blank">BBa_K2865013</a></td>
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</div>
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<td>Composite</td>
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<td>Left ITR-CMV-AR185-Poly(A)-Right ITR</td>
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</section>
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<td>Hu Miao</td>
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<td></td>
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<section class="paragragh" id="2nd">
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</tr>
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<tr>
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<header>
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<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2865014" target="_Blank">BBa_K2865014</a></td>
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<h2>See More </h2>
+
<td>Composite</td>
−
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<td>Left ITR-CMV-eGFP-Poly(A)-Right ITR</td>
−
</header>
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<td>Hu Miao</td>
−
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<td></td>
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<p>Integrated information about AR185 and BNP promotor posted on Demonstrate page. Or you can alternatively check the<a href="http://parts.igem.org/Part:BBa_K2865009" target="_blank"><b>(BBa_K2865009)</b></a> on the Registry of Standard Biological Parts website. </p>
+
</tr>
−
</section>
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<tr style="border-bottom: 1px #5d5d5d solid">
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2865015" target="_Blank">BBa_K2865015</a></td>
 +
<td>Composite</td>
 +
<td>Left ITR-BNP-eGFP-Poly(A)-Right ITR</td>
 +
<td>Hu Miao</td>
 +
<td></td>
 +
</tr>
 +
</table>
 +
</center>
 
</article>
 
</article>
  

Revision as of 06:47, 7 October 2018

Composite part

Favorite composite part

BNP-AR185 T2A eGFP-polyA

For this year’s iGEM competition, our favorite composite part is BNP-AR185-polyA. This part is composed of BNP promotor, AR185 linked with eGFP by T2A and poly A.

How BNP-AR185-polyA works

As has shown presiously, BNP promoter activity is related to the severity of heart failure. So we want to use ET-1, one stimulus factor, to imitate the environment of heart failure and induce BNP promoter expression. Then, the later sequence of the part, AR185, also can be expressed and fuction what it could specifically bind to RyR2 in rat cardiomyocytes and have the ability to inhibit PKA dependent S2808 phosphorylation in vitro.

See More

Integrated information about AR185 and BNP promotor posted on Demonstrate page. Or you can alternatively check the(BBa_K2865009) on the Registry of Standard Biological Parts website.

We listed other composite part we constructed this year below.

Name Type Description Designer Length
BBa_K2865006 Composite CMV promoter-eGFP-poly(A) Hu Miao
BBa_K2865008 Composite BNP promoter-eGFP-poly(A) Hu Miao
BBa_K2865009 Composite BNP-AR185-Poly(A) Hu Miao
BBa_K2865010 Composite CMV-AR185-Poly(A) Hu Miao
BBa_K2865012 Composite Left ITR-BNP-AR185-Poly(A)-Right ITR Hu Miao
BBa_K2865013 Composite Left ITR-CMV-AR185-Poly(A)-Right ITR Hu Miao
BBa_K2865014 Composite Left ITR-CMV-eGFP-Poly(A)-Right ITR Hu Miao
BBa_K2865015 Composite Left ITR-BNP-eGFP-Poly(A)-Right ITR Hu Miao
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