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| − | <p> A hundred years ago, a nightingale, created by Oscar Wilde, built a red rose for true love out of music by moonlight. To Wilde, the red rose with blood was his true art. Today, our E. coli, the incarnation of Wilde's nightingale, uses light and music to rebuild the rose and create a colorful and fragrant rose forest for scientists and artists. To us, the rose is the best combination of science and art.</p>
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| − | <p> This year, we found an excellent light control circuit using three sensors to sense light of different wavelengths and intensity and we added a RNAP system as resource allocator to connect the input and the output. Theoretically, it is possible to change the output into all kinds of genes to get E. coli able to do everything. In fact, we succeeded changing the output into four kinds of genes, and we have gotten E. coli that can express fluorescent proteins, chromoproteins, enzymes which combine with the chromogenic substance in the medium to produce color and odors. By combining different colors and allowing the genes of any two chromoproteins to be expressed in tandem in E. coli, we have gotten chromoproteins that are almost full-colors, including a green one which improved obviously in color over all current chromoproteins. The achievement of the creation of green support our theory of mixing primary colors to create more as artists do with pigments in painting. But how to control the percentage of the primary-color proteins and mix them? We found that when exposed to light of various wavelengths, three sensors were activated differently, leading to the expression of different proportions of red, green and blue florescent proteins. The satisfactory result provided a strong validation to our hypothesis. Besides, we also made some other interesting and beneficial attempts. For example, we developed a software to map the loudness and frequency of music onto the intensity and wavelength of light. Beforehand, we collect the correspondence of color and light, in case to simulate the actual condition of medium with computer. Inputting any music you want, we are able to transfer it into your special picture. Moreover, our roses painted by E. coli can even give offemit various sorts of smellspleasant odors, such as rain, lemon and flower by changing the output into scent genes and removing the original gene producing awful smell.</p>
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| − | <p>Finally, our E. coli produce different proportions of red, green and blue colors in response to light and music, realizing the painting of full-color roses that can even produce romantic flower scent, just like real rosess.</p>
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| − | <p>Besides，we plan to make an excellent and useful kit to collect almost all the genes related the color and light control of E. coli in iGEM and improve some of them, which will be more convenient for future reseachers to use.</p>
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| − | <p>In the end of Wilde's story, the rose, which stained by the nightingale's heart's blood and been regarded as the symbol of true love and marvelous art, was threw into the gutter. A hundred years later, we picked the rose again. What we strive to clarify is that, science is never all reality without any emotion, and art is never all style without any sincerity. Integrating idealistic humane feelings with logical genetic circuits to present an imaginative work in iGEM, also, the kit we developed enables other scientists and artists to do their own imaginative work more efficiently. We aim to bring forth a new perception of combiningof combination of art and science, to start a n absolutely new era of the creation of art-and-science combination. </p>
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