Difference between revisions of "Team:SCU-China"
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| − | <div class="contenthead">Story</div> | + | <div class="contenthead">Story |
| + | <p>Inspired by the modularization, call-and-return and do-not-reinvent-the-wheel philosophy in | ||
| + | computer programming design, we come up with the idea of using the dCas9 protein to manipulate | ||
| + | the expression of proteins and implement complex logic in a single E. coli cell this year. we | ||
| + | want to construct versatile “library strains” that contains many, and maybe redundant coding | ||
| + | sequences of commonly used proteins which are dormant by default; when we need to use the function | ||
| + | of particular already coded proteins, we just simply transform a much smaller “Minimid” containing | ||
| + | sgDNAs targeting the desired proteins into the library strain, and then utilizing the dCas9-sgRNA | ||
| + | complex to initiate the transcription then expression of the particular protein. | ||
| + | </p> | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 10:29, 8 October 2018
Inspired by the modularization, call-and-return and do-not-reinvent-the-wheel philosophy in computer programming design, we come up with the idea of using the dCas9 protein to manipulate the expression of proteins and implement complex logic in a single E. coli cell this year. we want to construct versatile “library strains” that contains many, and maybe redundant coding sequences of commonly used proteins which are dormant by default; when we need to use the function of particular already coded proteins, we just simply transform a much smaller “Minimid” containing sgDNAs targeting the desired proteins into the library strain, and then utilizing the dCas9-sgRNA complex to initiate the transcription then expression of the particular protein.