Difference between revisions of "Team:HZAU-China/Design"
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<div class="h1"><span class="xie">sifA</span> knock out</div> | <div class="h1"><span class="xie">sifA</span> knock out</div> | ||
| − | <p>SifA maintains the integrity of Salmonella-containing vacuole (SCV) where Salmonella survive and replicate1. The exsistence of SCV limits GSDMD-N275 releasing to cytoplasma. what else, growth of <span class="xie">ΔsifA</span> mutant is inhibited in macrophage2.Thus, we knock out the sifA gene in order to prevent the stability of SCV and reduce the virulence of Salmonella. <span class="xie">ΔsifA</span> mutant was cnstructed by using gene editing systerm based on two-step allelic exchange3.</p> | + | <p>SifA maintains the integrity of <i>Salmonella</i>-containing vacuole (SCV) where <i>Salmonella</i> survive and replicate1. The exsistence of SCV limits GSDMD-N275 releasing to cytoplasma. what else, growth of <span class="xie">ΔsifA</span> mutant is inhibited in macrophage2.Thus, we knock out the sifA gene in order to prevent the stability of SCV and reduce the virulence of <i>Salmonella</i>. <span class="xie">ΔsifA</span> mutant was cnstructed by using gene editing systerm based on two-step allelic exchange3.</p> |
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Revision as of 11:46, 9 October 2018
sifA knock out
SifA maintains the integrity of Salmonella-containing vacuole (SCV) where Salmonella survive and replicate1. The exsistence of SCV limits GSDMD-N275 releasing to cytoplasma. what else, growth of ΔsifA mutant is inhibited in macrophage2.Thus, we knock out the sifA gene in order to prevent the stability of SCV and reduce the virulence of Salmonella. ΔsifA mutant was cnstructed by using gene editing systerm based on two-step allelic exchange3.
Targeting
Regulation of pyroptosis
Reference
