Difference between revisions of "Team:FSU/Description"

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Can sound be used to induce gene expression in engineered E. coli? What frequencies, amplitudes, and length of time exposure effect gene expression the most?
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It is routine to trigger the production of a protein in engineered E. coli using a small molecule such as arabinose, lactose, and rhamnose.  Our project is focused on answering the question, “Can sound be used to induce gene expression in engineered E. coli?”  Prior iGEM projects demonstrated that sound can be used to activate eukaryotic cells.  Review of the scientific literature revealed projects where exposing E. coli to sound correlated with more vigorous growth in cell cultures and increased production of proteins.
 
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Today, it is routine to induce the expression of a gene in E. coli using small molecules such as arabinose, lactose, and rhamnose.  Can sound become a routine means to induce gene expression in engineered E. coli? Our project, Audiogenetics, offers a new foundational advance to the synthetic biology community. Slovenia’s Sonicell and SUS Tech’s Cearll’s Secret projects pursued a similar question in mammalian cells. We characterized putative promoters expected to be at end of a signal transduction pathway responsive to sound submitted by the 2008 Berkeley team In parallel, we studied genes that, according to published scientific reports, are up-regulated when bacteria are exposed to sound and identified the promoters expected to be involved in that system. These selected promoters were used to design new genetic devices that generate fluorescent reporters when the cells are exposed to sound.  We used our designed genetic devices to correlate levels of gene expression with frequency, amplitude, and exposure time.
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In 2008, the University of California, Berkeley (UC Berkeley) iGEM team contributed five genetic parts to the iGEM community.   The genetic parts were derived from DNA sequences found upstream from genes that were potentially upregulated when the cells were exposed to sound. The UC Berkeley iGEM team was unable to demonstrate definitively that the genetic parts could be used as sound inducible promoters of gene expression.  We decided to build on their work. We designed, analyzed, built, and tested new genetic devices that include the genetic parts from UC Berkeley based team intended to detect sound and trigger the expression of reporter proteins.  
 
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Audio induction has many possible impacts, the limit to which is still unclear. Our Human Practices team has started a discussion in the fields of molecular biology, brewery,  and law enforcement to explore the potential positive and negative impacts of using sound to induce gene expression.
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Members of the FSU iGEM team also investigated genes that were up-regulated when exposed to sound, according to other scientific literature. These signaling pathways and promoters were then analyzed and used to build new genetic devices which included reporter proteins that signal different levels of expression. All of our audio-inducible cells were tested at different frequencies and amplitudes to see which cells reported the highest level of reporter protein expression.  
 
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Audio induction has many possible impacts, the limit to which is still unclear. Our Human Practices team has started a discussion in the fields of molecular biology, brewery, and law enforcement to explore the potential positive and negative impacts of using sound to induce gene expression.
 
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Revision as of 03:00, 10 October 2018

Untitled-14

OVERVIEW

It is routine to trigger the production of a protein in engineered E. coli using a small molecule such as arabinose, lactose, and rhamnose. Our project is focused on answering the question, “Can sound be used to induce gene expression in engineered E. coli?” Prior iGEM projects demonstrated that sound can be used to activate eukaryotic cells. Review of the scientific literature revealed projects where exposing E. coli to sound correlated with more vigorous growth in cell cultures and increased production of proteins.

In 2008, the University of California, Berkeley (UC Berkeley) iGEM team contributed five genetic parts to the iGEM community. The genetic parts were derived from DNA sequences found upstream from genes that were potentially upregulated when the cells were exposed to sound. The UC Berkeley iGEM team was unable to demonstrate definitively that the genetic parts could be used as sound inducible promoters of gene expression. We decided to build on their work. We designed, analyzed, built, and tested new genetic devices that include the genetic parts from UC Berkeley based team intended to detect sound and trigger the expression of reporter proteins.

Members of the FSU iGEM team also investigated genes that were up-regulated when exposed to sound, according to other scientific literature. These signaling pathways and promoters were then analyzed and used to build new genetic devices which included reporter proteins that signal different levels of expression. All of our audio-inducible cells were tested at different frequencies and amplitudes to see which cells reported the highest level of reporter protein expression.

Audio induction has many possible impacts, the limit to which is still unclear. Our Human Practices team has started a discussion in the fields of molecular biology, brewery, and law enforcement to explore the potential positive and negative impacts of using sound to induce gene expression.