Difference between revisions of "Team:AHUT China/Notebook"
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<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;"> <span style="font-size: 24px"> Western</span> blot identification of proteins<br> CA2L203K CA2WT</p> <br> | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;"> <span style="font-size: 24px"> Western</span> blot identification of proteins<br> CA2L203K CA2WT</p> <br> | ||
| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/60/T--AHUT_China--93.1.png" width="971" height="167" alt=""/></div> | + | <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/60/T--AHUT_China--93.1.png" width="971" height="167" alt=""/></div> <br> |
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<div align="center"><img src="https://static.igem.org/mediawiki/2018/6/6e/T--AHUT_China--93.2.png" width="908" height="189" alt=""/></div> | <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/6e/T--AHUT_China--93.2.png" width="908" height="189" alt=""/></div> | ||
| − | <div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8b/T--AHUT_China--93.3.png" width="754" height="343" alt=""/></div> | + | <div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8b/T--AHUT_China--93.3.png" width="754" height="343" alt=""/></div><br> |
| + | <div class="container"> | ||
| + | <div class="section_title "> | ||
| + | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;"> <strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">9.05</strong></h2> | ||
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| + | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;"> <span style="font-size: 24px"> Colorimetric</span> enzyme activity<br> Add 250 μl saturated CO2 solution</p></p> | ||
| + | <br> | ||
| + | <div align="center"><img src="https://static.igem.org/mediawiki/2018/c/c4/T--AHUT_China--95.1.png" width="789" height="328" alt=""/></div> | ||
| + | <div align="center"><img src="https://static.igem.org/mediawiki/2018/8/82/T--AHUT_China--95.2.png" width="669" height="347" alt=""/></div> | ||
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Revision as of 11:52, 10 October 2018
8.29
Pick Clon
8.30
Pilot Expression of Protein
“IPTG+ 4h”“IPTG- 4h”
After the rocker culture is 4 H, add IPTG(final concentration is 0.5 mm) and then the rocker culture is 4 hours. Each of the two tubes absorbs 200 μL of bacterial fluid, and in the frozen centrifuge, it is 4 °C and 12000 rpm centrifuge. 10 minutes, go to the clear, It is resuspended with 100 μL 1x SDS Loading buffer, then heated in a water bath pot at 100 °C for 10 minutes and stored in a -20 °C refrigerator.
8.31
Protein extraction
CA2L203K
CA2WT
CA2L203K
9.01
Purification of Protein
Prior to purification
Purified
9.03
Western blot identification of proteins
CA2L203K CA2WT
9.04
Quantification of protein concentrations
9.05
Colorimetric enzyme activity
Add 250 μl saturated CO2 solution
