Difference between revisions of "Team:GreatBay China/Attributions"

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             </tr>
 
             </tr>
 
           </table>
 
           </table>
−
           <h5>✔: involved</h5><br>
+
           <h5>✔: involved</h5>
−
           <h5>✔✔: made main contribution</h5><br>
+
           <h5>✔✔: made main contribution<br><br>Items remarked by * means it was done by someone not student team members.</h5><br><br><br>
 +
 
 +
          <h2> Project Design</h2>
 +
          <h4> The initial idea of doing a project on catnip came from our advisor *Xuan Wang who mentioned to us the fact that catnip can be used as a green pesticide. Alexis Zeng did further research and saw the possibility of using a co-culture system for manufacturing. Lucinda Lin When we search for strategies to optimize the microbial production, our primary PI *Dr. Haoqian Zhang recommended the use of TALE stabilized promoters. </h4>
 +
          <br><br>
 +
 
 +
          <h2> Lab</h2>
 +
          <h4>  -Geraniol Production:</h4>
 +
          <ul>
 +
            <li>Design: Alexis Zeng</li>
 +
            <li>Molecular Cloning: Tara Yuan, Leo Li, Alexis Zeng, Leo Li, Rocky Yu, *Genscript (construction of pMVA-GPPS-GES)</li>
 +
            <li>Shake-flask fermentation: Alexis Zeng, Jesse Sun</li>
 +
            <li>GC Characterization: Sean Yao, Charles Wei</li>
 +
          </ul>
 +
          <h4>  -pTALE Promoters:</h4>
 +
          <ul>
 +
            <li>Design: Alexis Zeng, *Boxiang Wang</li>
 +
            <li>Molecular Cloning: Sean Yao, Rocky Yu, Tara Yuan, *Boxiang Wang (built the promoter library with us by SOE PCR)</li>
 +
            <li>Flow Cytometry Characterization: Tara Yuan, Sean Yao, Charles Wei</li>
 +
            <li>Genome Integration: Sean Yao, Alexis Zeng, *Yiming Dong (provided us with plasmids, taught us the usage, and performed PCR)</li>
 +
          </ul>
 +
          <h4>  -Yeast Gene Deletion:</h4>
 +
          <ul>
 +
            <li>Design: Tara Yuan, Alexis Zeng, *Boxiang Wang (taught Tara and Alexis principles for design sgRNA and homologous arm)</li>
 +
            <li>Molecular Cloning: Alexis Zeng, David Yang, Tara Yuan, Leo Li, Rocky Yu, Charles Wei</li>
 +
            <li>Verification: Alexis Zeng, *Xuanyu Zhao (deleted OYE2 by CRISPR while we achieved the same result by homologous recombination</li>
 +
          </ul>
 +
          <h4>  -Nepetalactol Production:</h4>
 +
          <ul>
 +
            <li>Design: Alexis Zeng, *Boxiang Wang (proposed a new strategy using biobrick assembly when we failed to construct the yeast vector using Gibson Assembly)</li>
 +
            <li>Molecular Cloning: Rocky Yu, Tara Yuan, Alexis Zeng</li>
 +
            <li>Shake-flask fermentation: Sean Yao, *Boxiang Wang (helped us to feed the yeast geraniol during night)</li>
 +
            <li>GC Characterization: Sean Yao, Charles Wei</li>
 +
          </ul>
 +
          <h4>  -Interlab:</h4>
 +
          <ul>
 +
            <li>Interlab:Sean Yao, Charles Wei, David Yang</li>
 +
          </ul>
 +
 
 +
          <br><br><br>
 +
 
 +
          <h2> Human Practice</h2>
 +
          <h4>  -Meet up:</h4>
 +
          <ul>
 +
            <li>Alexis Zeng, Lucinda Lin, and Sean Yao went to the iGEM Southern China Regional Meeting hosted by team SZU</li>
 +
            <li>Lucinda Lin and Sean Yao took part in the 5th Conference of China iGEMer Community</li>
 +
            <li>Alexis Zeng and Lucinda Lin organized the Shenzhen High School iGEM Meetup</li>
 +
            <li>Alexis Zeng, Lucinda Lin, Wade Xiao, Charles Wei, Rocky Yu participated the EurAsian Meet up</li>
 +
          </ul>
 +
          <h4>  -Integrated Human Practice:</h4>
 +
          <ul>
 +
            <li>Interview with Fenghua Li: Lucinda Lin, Rocky Yu, Wade Xiao</li>
 +
            <li>xfactory presentation: Lucinda Lin, Alexis Zeng</li>
 +
            <li>Costal City Interview: Eden Yu, Rocky Yu, Luncinda Lin, Alexis Zeng</li>
 +
            <li>Green Drinks Workshop: Eden Yu, Rocky Yu, Alexis Zeng</li>
 +
            <li>Interview with government: Rocky Yu</li>
 +
          </ul>
 +
          <h4>  -HP documentary:</h4>
 +
          <ul>
 +
            <li>Director: Rocky Yu</li>
 +
            <li>Participated: Lucinda Lin, Alexis Zeng, Eden Yu, Charles Wei, Leo Li</li>
 +
          </ul>
 +
          <h4>  -Public Engagement:</h4>
 +
          <ul>
 +
            <li>Makers Fair: Eden Yu, Alexis Zeng, Rocky Yu, Charles Wei</li>
 +
            <li>Maker space: Alexis Zeng, Lucinda Lin</li>
 +
            <li>Interview with neighborhood: Alexis Zeng</li>
 +
          </ul>
 +
          <h4>  -Applied Design:</h4>
 +
          <ul>
 +
            <li>Design: Alexis Zeng</li>
 +
            <li>3D model: Eden Yu</li>
 +
            <li>Construction: Eden Yu</li>
 +
          </ul>
 +
 
  
  

Revision as of 13:43, 10 October 2018

GBC-Attributions


mCATNIP2018@outlook.com
@Igem2018C

Attributions



Project Design Human Practice Lab Model Art Design Wiki Construction
Alexis Zeng ✔✔ ✔✔ ✔✔
Lucinda Lin ✔ ✔✔ ✔✔ ✔✔
Sean Yao ✔ ✔✔
Rocky Yu ✔✔ ✔✔
Tara Yuan ✔ ✔✔
Leo Li ✔ ✔✔
Charles Wei ✔ ✔✔ ✔✔
Wade Xiao ✔ ✔✔
Marie Wei ✔ ✔ ✔✔
David Yong ✔✔ ✔✔
Eden Yu ✔✔ ✔✔ ✔✔
Katrina Zhou ✔ ✔
Jessie Sun ✔
✔: involved
✔✔: made main contribution

Items remarked by * means it was done by someone not student team members.



Project Design

The initial idea of doing a project on catnip came from our advisor *Xuan Wang who mentioned to us the fact that catnip can be used as a green pesticide. Alexis Zeng did further research and saw the possibility of using a co-culture system for manufacturing. Lucinda Lin When we search for strategies to optimize the microbial production, our primary PI *Dr. Haoqian Zhang recommended the use of TALE stabilized promoters.



Lab

-Geraniol Production:

  • Design: Alexis Zeng
  • Molecular Cloning: Tara Yuan, Leo Li, Alexis Zeng, Leo Li, Rocky Yu, *Genscript (construction of pMVA-GPPS-GES)
  • Shake-flask fermentation: Alexis Zeng, Jesse Sun
  • GC Characterization: Sean Yao, Charles Wei

-pTALE Promoters:

  • Design: Alexis Zeng, *Boxiang Wang
  • Molecular Cloning: Sean Yao, Rocky Yu, Tara Yuan, *Boxiang Wang (built the promoter library with us by SOE PCR)
  • Flow Cytometry Characterization: Tara Yuan, Sean Yao, Charles Wei
  • Genome Integration: Sean Yao, Alexis Zeng, *Yiming Dong (provided us with plasmids, taught us the usage, and performed PCR)

-Yeast Gene Deletion:

  • Design: Tara Yuan, Alexis Zeng, *Boxiang Wang (taught Tara and Alexis principles for design sgRNA and homologous arm)
  • Molecular Cloning: Alexis Zeng, David Yang, Tara Yuan, Leo Li, Rocky Yu, Charles Wei
  • Verification: Alexis Zeng, *Xuanyu Zhao (deleted OYE2 by CRISPR while we achieved the same result by homologous recombination

-Nepetalactol Production:

  • Design: Alexis Zeng, *Boxiang Wang (proposed a new strategy using biobrick assembly when we failed to construct the yeast vector using Gibson Assembly)
  • Molecular Cloning: Rocky Yu, Tara Yuan, Alexis Zeng
  • Shake-flask fermentation: Sean Yao, *Boxiang Wang (helped us to feed the yeast geraniol during night)
  • GC Characterization: Sean Yao, Charles Wei

-Interlab:

  • Interlab:Sean Yao, Charles Wei, David Yang



Human Practice

-Meet up:

  • Alexis Zeng, Lucinda Lin, and Sean Yao went to the iGEM Southern China Regional Meeting hosted by team SZU
  • Lucinda Lin and Sean Yao took part in the 5th Conference of China iGEMer Community
  • Alexis Zeng and Lucinda Lin organized the Shenzhen High School iGEM Meetup
  • Alexis Zeng, Lucinda Lin, Wade Xiao, Charles Wei, Rocky Yu participated the EurAsian Meet up

-Integrated Human Practice:

  • Interview with Fenghua Li: Lucinda Lin, Rocky Yu, Wade Xiao
  • xfactory presentation: Lucinda Lin, Alexis Zeng
  • Costal City Interview: Eden Yu, Rocky Yu, Luncinda Lin, Alexis Zeng
  • Green Drinks Workshop: Eden Yu, Rocky Yu, Alexis Zeng
  • Interview with government: Rocky Yu

-HP documentary:

  • Director: Rocky Yu
  • Participated: Lucinda Lin, Alexis Zeng, Eden Yu, Charles Wei, Leo Li

-Public Engagement:

  • Makers Fair: Eden Yu, Alexis Zeng, Rocky Yu, Charles Wei
  • Maker space: Alexis Zeng, Lucinda Lin
  • Interview with neighborhood: Alexis Zeng

-Applied Design:

  • Design: Alexis Zeng
  • 3D model: Eden Yu
  • Construction: Eden Yu