Difference between revisions of "Team:SSTi-SZGD/Parts"

m
Line 155: Line 155:
 
<tr>
 
<tr>
 
<td><a href="http://parts.igem.org/Part:BBa_K2593001">BBa_K2593001</a></td>
 
<td><a href="http://parts.igem.org/Part:BBa_K2593001">BBa_K2593001</a></td>
−
<td>Basic<!--{cn}基本--></td>
+
<td>coding<!--{cn}编码--></td>
 
<td>Hyaluronic acid synthase protein<!--{cn}透明质酸合成酶蛋白--></td>
 
<td>Hyaluronic acid synthase protein<!--{cn}透明质酸合成酶蛋白--></td>
 
<td>Hong Chen<!--{cn}陈红--></td>
 
<td>Hong Chen<!--{cn}陈红--></td>
Line 162: Line 162:
 
<tr>
 
<tr>
 
<td><a href="http://parts.igem.org/Part:BBa_K2593003">BBa_K2593003</a></td>
 
<td><a href="http://parts.igem.org/Part:BBa_K2593003">BBa_K2593003</a></td>
−
<td>Basic<!--{cn}基本--></td>
+
<td>coding<!--{cn}编码--></td>
 
<td>Signal peptide<!--{cn}信号肽--></td>
 
<td>Signal peptide<!--{cn}信号肽--></td>
 
<td>Hong Chen<!--{cn}陈红--></td>
 
<td>Hong Chen<!--{cn}陈红--></td>
Line 169: Line 169:
 
<tr>
 
<tr>
 
<td><a href="http://parts.igem.org/Part:BBa_K2593004">BBa_K2593004</a></td>
 
<td><a href="http://parts.igem.org/Part:BBa_K2593004">BBa_K2593004</a></td>
−
<td>Basic<!--{cn}基本--></td>
+
<td>coding<!--{cn}编码--></td>
 
<td>Leech Hyaluronic acid hydrolase protein (58KDa)<!--{cn}水蛭透明质酸水解酶蛋白（58 Kda）--></td>
 
<td>Leech Hyaluronic acid hydrolase protein (58KDa)<!--{cn}水蛭透明质酸水解酶蛋白（58 Kda）--></td>
 
<td>Huimin Li<!--{cn}李慧敏--></td>
 
<td>Huimin Li<!--{cn}李慧敏--></td>
Line 176: Line 176:
 
<tr>
 
<tr>
 
<td><a href="http://parts.igem.org/Part:BBa_K2593009">BBa_K2593009</a></td>
 
<td><a href="http://parts.igem.org/Part:BBa_K2593009">BBa_K2593009</a></td>
−
<td>Basic<!--{cn}基本--></td>
+
<td>coding<!--{cn}编码--></td>
 
<td>Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus<!--{cn}含信号肽和6 His的水蛭Haase编码基因(n-端)--></td>
 
<td>Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus<!--{cn}含信号肽和6 His的水蛭Haase编码基因(n-端)--></td>
 
<td>Huimin Li<!--{cn}李慧敏--></td>
 
<td>Huimin Li<!--{cn}李慧敏--></td>

Revision as of 08:05, 11 October 2018

Overview
This year, we submitted seven new parts in total, including four basic parts and three composite parts. We took advantage on the BGI China special gene synthesis promotion offer in August this year to synthesize our basic parts, such as coding sequence, RBS, promoter and signal peptide separately. The composite parts were generated using a combination of PCR, restriction ligation and 3A assembly methods. All submitted parts are RFC10 compatible.
Improved parts
In our project, we added to the characterization of  an existing Biobrick , design by Philip Hartz from iGEM Saarland 2014 . Our P43-tuaD-gtaB-T1 composite part was designed based on the  BioBrick created by Saarland with some alterations . For example, co-expression of tuaD-gtaB as an operon under the control a constitutive promoter so that recombinant B. Subtilis 168 produces elevated level of HA.
All parts
Name Type Description Designer Length
BBa_K2593001 coding Hyaluronic acid synthase protein Hong Chen 1254
BBa_K2593003 coding Signal peptide Hong Chen 99
BBa_K2593004 coding Leech Hyaluronic acid hydrolase protein (58KDa) Huimin Li 1470
BBa_K2593009 coding Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus Huimin Li 1686
BBa_K2593006 composite Leech Hyaluronic acid hydrolase with inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator Yongtao Lin 1962
BBa_K2593007 composite Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator. Yongtao Lin 2672
BBa_K2593008 composite UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator. Hong Chen 2796
Basic parts
Name Type Description Designer Length
BBa_K2593001 coding Hyaluronic acid synthase protein Hong Chen 1254
BBa_K2593003 coding Signal peptide Hong Chen 99
BBa_K2593004 coding Leech Hyaluronic acid hydrolase protein (58KDa) Huimin Li 1470
BBa_K2593009 coding Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus Huimin Li 1686
Composite parts
Name Type Description Designer Length
BBa_K2593006 composite Leech Hyaluronic acid hydrolase with an inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator. Yongtao Lin 1962
BBa_K2593007 composite Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator. Yongtao Lin 2672
BBa_K2593008 composite UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator. Hong Chen 2796

Copyright © 2018 SSTi-SZGD All Rights Reserved.
Copyright © 2018 SSTi-SZGD All Rights Reserved.