Difference between revisions of "Team:SSTi-SZGD/Basic Part"

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<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:SSTi-SZGD/css/Parts?action=raw&ctype=text/css"/>
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<script src="https://2018.igem.org/Team:SSTi-SZGD/js/Parts?action=raw&ctype=text/javascript" type="text/javascript" charset="utf-8"></script>
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<body>
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<div class="page_content">
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<div class="Banner"></div>
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<!--Content-->
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<div class="container Content">
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<!--Overview-->
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<section class="Overview col-xs-12 col-md-10">
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<div class="title">Overview<!--{cn}概述--></div>
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<div class="content abstract">
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This year, we submitted seven new parts in total, including four basic parts and three composite parts. We took advantage on the BGI China special gene synthesis promotion offer in August this year to synthesize our basic parts, such as coding sequence, RBS, promoter and signal peptide separately. The composite parts were generated using a combination of PCR, restriction ligation and 3A assembly methods. All submitted parts are RFC10 compatible.
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<!--{cn}今年，我们共提交了7个新的部分，包括4个基本部分和3个综合部分。我们利用BGI中国公司今年8月推出的特殊基因合成产品来合成我们的基础部件，分别合成了编码序列、RBS、启动子和信号肽等基本部分。采用聚合酶链反应(PCR)、限制性内切酶连接和3a装配相结合的方法制备复合构件。所有提交的部件都与RFC 10兼容。-->
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</section>
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<!--Improved part-->
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<section id="Improved_part" class="Improved_part col-xs-12 col-md-10">
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<div class="subtitle">
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<span>Improved parts<!--{cn}改进部分--></span>
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</div>
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<div class="content abstract">
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In our project, we added to the characterization of 
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<!--{cn}在我们的项目中，我们添加了现有的-->
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<a href="http://parts.igem.org/Part:BBa_K1469009">an existing Biobrick<!--{cn}BioBrick的特性--></a>
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, design by Philip Hartz from iGEM Saarland 2014 . Our P43-tuaD-gtaB-T1 composite part was designed based on the 
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<!--{cn}，由2014年iGEM Saarland的Philip Hartz设计。我们的p43-tuad-gtab-t1复合部件是在Saarland的-->
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<a href="http://parts.igem.org/Part:BBa_K2593008">BioBrick created by<!--{cn}BioBrick基础上设计的--></a>
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Saarland with some alterations . For example, co-expression of tuaD-gtaB as an operon under the control a constitutive promoter so that recombinant
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<!--{cn}，并作了一些改动。例如，将tuad-gtab作为操纵子的共同表达作为控制下的一个本构启动子以至于重组-->
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<i>B. Subtilis 168<!--{cn}枯草芽孢杆菌168--></i>
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produces elevated level of HA.
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<!--{cn}产生较高水平的透明质酸。-->
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</div>
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</section>
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<!--All parts-->
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<section id="All_parts" class="All_parts col-xs-12 col-md-10">
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<div class="subtitle">
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<span>All parts<!--{cn}所有部分--></span>
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</div>
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<div class="content table-responsive">
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<table class="table">
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<thead>
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<tr>
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<th>Name<!--{cn}名称--></th>
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<th>Type<!--{cn}类型--></th>
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<th>Description<!--{cn}描述--></th>
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<th>Designer<!--{cn}设计者--></th>
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<th>Length<!--{cn}长度(bp)--></th>
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</tr>
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</thead>
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<tbody>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593001">BBa_K2593001</a></td>
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<td>coding<!--{cn}编码--></td>
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<td>Hyaluronic acid synthase protein<!--{cn}透明质酸合成酶蛋白--></td>
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<td>Hong Chen<!--{cn}陈红--></td>
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<td>1254</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593003">BBa_K2593003</a></td>
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<td>coding<!--{cn}编码--></td>
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<td>Signal peptide<!--{cn}信号肽--></td>
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<td>Hong Chen<!--{cn}陈红--></td>
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<td>99</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593004">BBa_K2593004</a></td>
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<td>coding<!--{cn}编码--></td>
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<td>Leech Hyaluronic acid hydrolase protein (58KDa)<!--{cn}水蛭透明质酸水解酶蛋白（58 Kda）--></td>
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<td>Huimin Li<!--{cn}李慧敏--></td>
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<td>1470</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593009">BBa_K2593009</a></td>
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<td>coding<!--{cn}编码--></td>
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<td>Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus<!--{cn}含信号肽和6 His的水蛭Haase编码基因(n-端)--></td>
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<td>Huimin Li<!--{cn}李慧敏--></td>
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<td>1686</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593006">BBa_K2593006</a></td>
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<td>composite<!--{cn}综合--></td>
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<td>Leech Hyaluronic acid hydrolase with inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator<!--{cn}水蛭透明质酸水解酶具有诱导启动子，RBS，一个输出信号肽，6His和终止子--></td>
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<td>Yongtao Lin<!--{cn}林永涛--></td>
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<td>1962</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593007">BBa_K2593007</a></td>
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<td>composite<!--{cn}综合--></td>
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<td>Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator.<!--{cn}具有诱导启动子、RBS和终止子的透明质酸合成酶蛋白--></td>
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<td>Yongtao Lin<!--{cn}林永涛--></td>
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<td>2672</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593008">BBa_K2593008</a></td>
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<td>composite<!--{cn}综合--></td>
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<td>UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator.<!--{cn}UDP-葡萄糖脱氢酶和UDP-果糖焦磷酸化酶具有组成性启动子RBS和终止子--></td>
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<td>Hong Chen<!--{cn}陈红--></td>
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<td>2796</td>
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</tr>
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</tbody>
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</table>
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</div>
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</section>
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<!--Basic part-->
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<section id="Basic_part" class="Basic_part col-xs-12 col-md-10">
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<div class="subtitle">
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<span>Basic parts<!--{cn}基本配件--></span>
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</div>
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<div class="content table-responsive">
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<table class="table">
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<thead>
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<tr>
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<th>Name<!--{cn}名称--></th>
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<th>Type<!--{cn}类型--></th>
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<th>Description<!--{cn}描述--></th>
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<th>Designer<!--{cn}设计者--></th>
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<th>Length<!--{cn}长度(bp)--></th>
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</tr>
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</thead>
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<tbody>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593001">BBa_K2593001</a></td>
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<td>coding<!--{cn}编码--></td>
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<td>Hyaluronic acid synthase protein<!--{cn}透明质酸合成酶蛋白--></td>
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<td>Hong Chen<!--{cn}陈红--></td>
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<td>1254</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593003">BBa_K2593003</a></td>
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<td>coding<!--{cn}编码--></td>
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<td>Signal peptide<!--{cn}信号肽--></td>
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<td>Hong Chen<!--{cn}陈红--></td>
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<td>99</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593004">BBa_K2593004</a></td>
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<td>coding<!--{cn}编码--></td>
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<td>Leech Hyaluronic acid hydrolase protein (58KDa)<!--{cn}水蛭透明质酸水解酶蛋白（58 Kda）--></td>
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<td>Huimin Li<!--{cn}李慧敏--></td>
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<td>1470</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593009">BBa_K2593009</a></td>
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<td>coding<!--{cn}编码--></td>
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<td>Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus<!--{cn}含信号肽和6 His的水蛭Haase编码基因(n-端)--></td>
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<td>Huimin Li<!--{cn}李慧敏--></td>
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<td>1686</td>
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</tr>
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</tbody>
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</table>
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</div>
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</section>
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<!--Composite part-->
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<section id="Composite_part" class="Composite_part col-xs-12 col-md-10">
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<div class="subtitle">
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<span>Composite parts<!--{cn}复合零件--></span>
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</div>
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<div class="content table-responsive">
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<table class="table">
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<thead>
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<tr>
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<th>Name<!--{cn}名称--></th>
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<th>Type<!--{cn}类型--></th>
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<th>Description<!--{cn}描述--></th>
 +
<th>Designer<!--{cn}设计者--></th>
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<th>Length<!--{cn}长度(bp)--></th>
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</tr>
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</thead>
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<tbody>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593006">BBa_K2593006</a></td>
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<td>composite<!--{cn}综合--></td>
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<td>Leech Hyaluronic acid hydrolase with an inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator.<!--{cn}水蛭透明质酸水解酶具有诱导启动子，RBS，一个输出信号肽，6His和终止子--></td>
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<td>Yongtao Lin<!--{cn}林永涛--></td>
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<td>1962</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593007">BBa_K2593007</a></td>
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<td>composite<!--{cn}综合--></td>
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<td>Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator.<!--{cn}具有诱导启动子、RBS和终止子的透明质酸合成酶蛋白--></td>
 +
<td>Yongtao Lin<!--{cn}林永涛--></td>
 +
<td>2672</td>
 +
</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2593008">BBa_K2593008</a></td>
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<td>composite<!--{cn}综合--></td>
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<td>UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator.<!--{cn}UDP-葡萄糖脱氢酶和UDP-果糖焦磷酸化酶具有组成性启动子RBS和终止子--></td>
 +
<td>Hong Chen<!--{cn}陈红--></td>
 +
<td>2796</td>
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</tr>
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</tbody>
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</table>
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</div>
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</section>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Basic Parts</h1>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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<h3>Best Basic Part Special Prize</h3>
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<p> To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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Revision as of 08:06, 11 October 2018

Overview
This year, we submitted seven new parts in total, including four basic parts and three composite parts. We took advantage on the BGI China special gene synthesis promotion offer in August this year to synthesize our basic parts, such as coding sequence, RBS, promoter and signal peptide separately. The composite parts were generated using a combination of PCR, restriction ligation and 3A assembly methods. All submitted parts are RFC10 compatible.
Improved parts
In our project, we added to the characterization of  an existing Biobrick , design by Philip Hartz from iGEM Saarland 2014 . Our P43-tuaD-gtaB-T1 composite part was designed based on the  BioBrick created by Saarland with some alterations . For example, co-expression of tuaD-gtaB as an operon under the control a constitutive promoter so that recombinant B. Subtilis 168 produces elevated level of HA.
All parts
Name Type Description Designer Length
BBa_K2593001 coding Hyaluronic acid synthase protein Hong Chen 1254
BBa_K2593003 coding Signal peptide Hong Chen 99
BBa_K2593004 coding Leech Hyaluronic acid hydrolase protein (58KDa) Huimin Li 1470
BBa_K2593009 coding Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus Huimin Li 1686
BBa_K2593006 composite Leech Hyaluronic acid hydrolase with inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator Yongtao Lin 1962
BBa_K2593007 composite Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator. Yongtao Lin 2672
BBa_K2593008 composite UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator. Hong Chen 2796
Basic parts
Name Type Description Designer Length
BBa_K2593001 coding Hyaluronic acid synthase protein Hong Chen 1254
BBa_K2593003 coding Signal peptide Hong Chen 99
BBa_K2593004 coding Leech Hyaluronic acid hydrolase protein (58KDa) Huimin Li 1470
BBa_K2593009 coding Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus Huimin Li 1686
Composite parts
Name Type Description Designer Length
BBa_K2593006 composite Leech Hyaluronic acid hydrolase with an inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator. Yongtao Lin 1962
BBa_K2593007 composite Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator. Yongtao Lin 2672
BBa_K2593008 composite UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator. Hong Chen 2796

Copyright © 2018 SSTi-SZGD All Rights Reserved.
Copyright © 2018 SSTi-SZGD All Rights Reserved.