Difference between revisions of "Team:DLUT China B/Parts"

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    <title>Parts</title>
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        <div class="moduleTitle">Parts</div>
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            <span>Parts</span>
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            <p>For this year’s iGEM competition, our team has chosen two new parts to finish our project.</p>
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            <p>The first one is Nanobody with aldehyde-based precursor label, can be converted to fixed-point
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                aldehyde-modified Nano (BBa_K2772000). It is anti-beta2 microglobulin nanobody with aldehyde-based
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                precursor label, and it can be converted to fixed-point aldehyde-modified Nanobody by
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                formylglycine-generating enzyme (FGE). It contains two sections: nanobody and ald tag, which is
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                precursor
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                of aldehyde label. Firstly it can cambein with beta2 microglobulin because of antigen-antibody
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                interaction. Then its special lable allow fixed point fixing or other fixed point decoration
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                possible.</p>
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            <img src="https://static.igem.org/mediawiki/2018/c/cc/T--DLUT_China_B--Parts1.png">
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            <p>Another one is Formylglycine-generating enzyme(BBa_K2772018). FGE can specifically identify ald-Tag,
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                which is consisted of 6 base pair or 13 base pair, then it oxidze sulfhydryl group of sulfhydryl group
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                to aldehyde group. Conveniently, it can be used for in vivo catalysis of the substrate label of the
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                precursor, i.e. higher conversion can be achieved without purification separation.</p>
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            <img src="https://static.igem.org/mediawiki/2018/1/11/T--DLUT_China_B--Parts2.png">
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            <p>Here is the list of our submitted parts.</p>
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                    <th>Name</th>
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                    <th>Description</th>
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                    <th>Length</th>
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                </tr>
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                </thead>
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                <tr>
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                    <td>BBa_K2772000</td>
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                    <td>Nanobody with aldehyde-based precursor label</td>
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                    <td>528bp</td>
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                </tr>
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                <tr>
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                    <td>BBa_K2772018</td>
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                    <td>Formylglycine-generating enzyme</td>
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                    <td>863bp</td>
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                </tr>
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            </table>
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<h1>Parts</h1>
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        <div id="address">
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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            <h3><i class="fas fa-map-marker-alt"></i> Address</h3>
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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            <p>No.2 Linggong Road, Ganjingzi District, Dalian City, Liaoning Province, P.R.C., 116024</p>
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            <h3><i class="fas fa-at"></i> E-mail</h3>
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            <p>igem_dlut_china_B#163.com (Please replace # with @)</p>
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        </div>
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        <div id="follow">
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            <h3>Follow Us</h3>
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                <p><i class="fab fa-weixin"></i> WeChat</p>
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                <p></p>
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                <p><i class="fab fa-weibo"></i> Weibo</p>
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                <div></div>
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                <div><img src="https://static.igem.org/mediawiki/2018/6/6b/T--DLUT_China_B--weibo.png" width="100"></div>
  
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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<h3>Inspiration</h3>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h3>Part Table </h3>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM18 DLUT_China_B</groupparts>
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Revision as of 08:10, 11 October 2018

Parts


Parts
Parts

For this year’s iGEM competition, our team has chosen two new parts to finish our project.

The first one is Nanobody with aldehyde-based precursor label, can be converted to fixed-point aldehyde-modified Nano (BBa_K2772000). It is anti-beta2 microglobulin nanobody with aldehyde-based precursor label, and it can be converted to fixed-point aldehyde-modified Nanobody by formylglycine-generating enzyme (FGE). It contains two sections: nanobody and ald tag, which is precursor of aldehyde label. Firstly it can cambein with beta2 microglobulin because of antigen-antibody interaction. Then its special lable allow fixed point fixing or other fixed point decoration possible.

Another one is Formylglycine-generating enzyme(BBa_K2772018). FGE can specifically identify ald-Tag, which is consisted of 6 base pair or 13 base pair, then it oxidze sulfhydryl group of sulfhydryl group to aldehyde group. Conveniently, it can be used for in vivo catalysis of the substrate label of the precursor, i.e. higher conversion can be achieved without purification separation.

Here is the list of our submitted parts.

Name Description Length
BBa_K2772000 Nanobody with aldehyde-based precursor label 528bp
BBa_K2772018 Formylglycine-generating enzyme 863bp