Difference between revisions of "Team:TUDelft/Parts/Main"
Nicolebennis (Talk | contribs) |
Nicolebennis (Talk | contribs) |
||
| Line 33: | Line 33: | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
<td>♥</td> | <td>♥</td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643000" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643000" target="_blank" class="jnnbl">BBa_K2643000</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>dxCas9-linker-Tn5 fusion protein (HIS Tag)</td> | <td>dxCas9-linker-Tn5 fusion protein (HIS Tag)</td> | ||
| Line 40: | Line 40: | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
<td>♥</td> | <td>♥</td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643001" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643001" target="_blank" class="jnnbl">BBa_K2643001</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>dxCas9 (HIS Tag)</td> | <td>dxCas9 (HIS Tag)</td> | ||
| Line 47: | Line 47: | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
<td>♥</td> | <td>♥</td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643002" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643002" target="_blank" class="jnnbl">BBa_K2643002</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Tn5 Transposase (HIS Tagged)</td> | <td>Tn5 Transposase (HIS Tagged)</td> | ||
| Line 54: | Line 54: | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
<td></td> | <td></td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643003" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643003" target="_blank" class="jnnbl">BBa_K2643003</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Glycine Helical peptide Linker (GHL</td> | <td>Glycine Helical peptide Linker (GHL</td> | ||
| Line 61: | Line 61: | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
<td>♥</td> | <td>♥</td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643004" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643004" target="_blank" class="jnnbl">BBa_K2643004</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Coding region (CDS) of human erythropoietin (EPO) hormone</td> | <td>Coding region (CDS) of human erythropoietin (EPO) hormone</td> | ||
| Line 68: | Line 68: | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
<td></td> | <td></td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643005" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643005" target="_blank" class="jnnbl">BBa_K2643005</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Human EPO gene with artificial intron (615 bp)</td> | <td>Human EPO gene with artificial intron (615 bp)</td> | ||
| − | <td> | + | <td>TU Delft 2018</td> |
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
<td></td> | <td></td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643006" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643006" target="_blank" class="jnnbl">BBa_K2643006</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Human EPO gene with artificial intron (135 bp)</td> | <td>Human EPO gene with artificial intron (135 bp)</td> | ||
| Line 82: | Line 82: | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
<td></td> | <td></td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643007" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643007" target="_blank" class="jnnbl">BBa_K2643007</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Human EPO gene with 2 artificial introns (615 + 135 bp)</td> | <td>Human EPO gene with 2 artificial introns (615 + 135 bp)</td> | ||
| Line 89: | Line 89: | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
<td></td> | <td></td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643008" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643008" target="_blank" class="jnnbl">BBa_K2643008</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Human EPO gene with mutation 1 (5 bp)</td> | <td>Human EPO gene with mutation 1 (5 bp)</td> | ||
| Line 96: | Line 96: | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
<td></td> | <td></td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643009" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643009" target="_blank" class="jnnbl">BBa_K2643009</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Human EPO gene with mutations 2 (12 bp)</td> | <td>Human EPO gene with mutations 2 (12 bp)</td> | ||
| Line 103: | Line 103: | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
<td></td> | <td></td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643010" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643010" target="_blank" class="jnnbl">BBa_K2643010</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>gRNA expression cassette for CRISPR-targeting <i>lacZ</i></td> | <td>gRNA expression cassette for CRISPR-targeting <i>lacZ</i></td> | ||
| Line 110: | Line 110: | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
<td></td> | <td></td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643011" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643011" target="_blank" class="jnnbl">BBa_K2643011</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>Mosaic Ends-flanked Kanamycin cassette and RFP</td> | <td>Mosaic Ends-flanked Kanamycin cassette and RFP</td> | ||
| Line 117: | Line 117: | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
<td></td> | <td></td> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2643012" target="_blank" class=" | + | <td><a href="http://parts.igem.org/Part:BBa_K2643012" target="_blank" class="jnnbl">BBa_K2643012</a></td> |
<td>Basic</td> | <td>Basic</td> | ||
<td>T7p expressing sgRNA targeting the EPO coding sequence</td> | <td>T7p expressing sgRNA targeting the EPO coding sequence</td> | ||
Revision as of 11:04, 11 October 2018
stuff to write here
1. Parts Overview
| Fave Part | Name | Type | Description | Designer |
|---|---|---|---|---|
| ♥ | BBa_K2643000 | Composite | dxCas9-linker-Tn5 fusion protein (HIS Tag) | TU Delft 2018 |
| ♥ | BBa_K2643001 | Basic | dxCas9 (HIS Tag) | TU Delft 2018 |
| ♥ | BBa_K2643002 | Basic | Tn5 Transposase (HIS Tagged) | TU Delft 2018 |
| BBa_K2643003 | Basic | Glycine Helical peptide Linker (GHL | TU Delft 2018 | |
| ♥ | BBa_K2643004 | Basic | Coding region (CDS) of human erythropoietin (EPO) hormone | TU Delft 2018 |
| BBa_K2643005 | Basic | Human EPO gene with artificial intron (615 bp) | TU Delft 2018 | |
| BBa_K2643006 | Basic | Human EPO gene with artificial intron (135 bp) | TU Delft 2018 | |
| BBa_K2643007 | Basic | Human EPO gene with 2 artificial introns (615 + 135 bp) | TU Delft 2018 | |
| BBa_K2643008 | Basic | Human EPO gene with mutation 1 (5 bp) | TU Delft 2018 | |
| BBa_K2643009 | Basic | Human EPO gene with mutations 2 (12 bp) | TU Delft 2018 | |
| BBa_K2643010 | Basic | gRNA expression cassette for CRISPR-targeting lacZ | TU Delft 2018 | |
| BBa_K2643011 | Basic | Mosaic Ends-flanked Kanamycin cassette and RFP | TU Delft 2018 | |
| BBa_K2643012 | Basic | T7p expressing sgRNA targeting the EPO coding sequence | TU Delft 2018 |
2. Basic Parts
| Fave Part | Name | Type | Description | Designer |
|---|---|---|---|---|
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
3. Composite Parts
| Fave Part | Name | Type | Description | Designer |
|---|---|---|---|---|
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
4. Part Collection
| Fave Part | Name | Type | Description | Designer |
|---|---|---|---|---|
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |
| Fav | BioBrick#1 | Basic Part | Hay this part is not yet created | Me myself and I |