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Revision as of 11:35, 11 October 2018
Sodium Dodecyl Sulphate (SDS) a component in shampoos, toothpastes and detergents accumulates in water bodies. It is toxic and foams, causing sewage problems
Our engineered E.coli DH5-α extracellularly releases alkyl sulfatase via attachment of N-terminal secretion signal sequence peIB
The action of alkyl sulfatase on SDS is that of degradation. The steps are stopped at 1-dodecanol
1-dodecanol is separated