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| − | ">INTERLAB STUDY</h3> | + | ">Biological Design: The Golden Braid Assembly</h3> |
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| − | ">INTRODUCTION</h4>
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| − | <!--<h5 style="
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| − | ">Lorem ipsum</h5>-->
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| − | ">Lorem ipsum</h6>-->
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| − | <p style="
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| − | color: #353535;">Do you imagine doing an experiment that could not be repeated? What if, after performing the same experiment several times, you obtain different results each time? This is a common problem throughout almost all laboratories in the entire world. A challenge, not just for Synthetic Biology but for any type of science, is taking reliable and repeatable measurements.
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| − | </p>
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| − | <p style="
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| − | color: #353535;">Over the past four years, the iGem Measurement Committee has been developing a series of experiments to make the biggest interlaboratory studies ever done in synthetic biology, and, in that way, try to fix all possible variables within a particular protocol.
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| − | <p style="
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| − | </p>
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| − | <h4 style="
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| − | color: #353535;
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| − | font-size: 21px;
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| − | ">WHAT IS THIS YEAR'S GOAL?</h4>
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| − | <p style="
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| − | color: #353535;">
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| − | To know if there is any chance to reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or c-forming units (CFUs) instead of optical density (OD).
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| − | </p>
| + | |
| − | <p style="
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| − | line-height: 1.7;
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| − | color: #353535;"> In order to compute the cell count in our samples, we will use two orthogonal approaches:
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| − | </p>
| + | |
| − | <h5 style="
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| − | font-size: 16px;
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| − | font-weight: 750;
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| − | ">Approach 1: Converting between absorbance of cells to absorbance of a known concentration of beads</h5>
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| − | <p style="
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| + | |
| − | The theory under how absorbance is measured is quite simple: a liquid sample of cells scatter light in a way or another depending on the number of cells this sample contains. The Committee provides us a sample with silica beads which are almost the same size and shape as a typical E. coli cell. So, when mixed with water, we obtain a liquid that should scatter light in a similar way as our E. coli sample does.
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| − | | + | |
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| − | </p> | + | |
| − | <p style="
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| − | line-height: 1.7;
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| − | margin-bottom: 0;
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| − | color: #353535;">
| + | |
| − | Because we know the concentration of beads, the absorbance measurement from a particular cell sample could be converted into an “equivalent concentration of beads” measurement, so that they are more universal and comparable measurements between different labs.
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <h5 style="
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| − | color: #353535;
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| − | font-size: 16px;
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| − | font-weight: 750;
| + | |
| − | ">Approach 2: Counting c-forming units (CFUs) from the sample</h5>
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| − | | + | |
| − | <p style="
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| − | line-height: 1.7;
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| − | margin-bottom: 0;
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| − | color: #353535;">
| + | |
| − | This method relies on the idea that every grown c in our plate comes from a single cell. So, if we spread a known cell culture volume over an agar plate and then we count the number of colonies, we should have an idea on how many cells our liquid sample had. We will have to determine the number of CFUs in positive and negative control samples in order to compute a conversion factor from absorbance to CFU.
| + | |
| − | </p>
| + | |
| − | <p style="
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| − | line-height: 1.7;
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| − | margin-bottom: 0;
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| − | color: #353535;">
| + | |
| − | </p>
| + | |
| − | <h4 style="
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| − | color: #353535;
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| − | font-weight: 700;
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| − | font-size: 21px;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | ">PLATE READER SETUP</h4>
| + | |
| − | <h5 style="
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| − | color: #353535;
| + | |
| − | font-size: 16px;
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| − | font-weight: 750;
| + | |
| − | ">ABSORBANCE600</h5>
| + | |
| − | <p style="
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| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;">Absorbance Endpoint
| + | |
| − | | + | |
| − | </p>
| + | |
| − | <p style="
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| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
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| − | color: #353535;">Full Plate
| + | |
| − | </p>
| + | |
| − | <p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;">Wavelengths: 600
| + | |
| − | </p>
| + | |
| − | <p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;">Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 8
| + | |
| − | </p>
| + | |
| − | <p style="
| + | |
| − | line-height: 1.7;
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| − | margin-bottom: 0;
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| − | color: #353535;">
| + | |
| − | </p>
| + | |
| − | <h5 style="
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| − | color: #353535;
| + | |
| − | font-size: 16px;
| + | |
| − | font-weight: 750;
| + | |
| − | ">FLUORESCENCE</h5>
| + | |
| − | <p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> Excitation: 485, Emission: 528
| + | |
| − | </p><p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> Optics: Top, Gain: 50
| + | |
| − | </p><p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> Light Source: Xenon Flash, Lamp Energy: High
| + | |
| − | </p><p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 10
| + | |
| − | </p><p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> Read Height: 7 mm
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | <h4 style="
| + | |
| − | color: #353535;
| + | |
| − | font-weight: 700;
| + | |
| − | font-size: 21px;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | ">USED PARTS</h4>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Device</th>
| + | |
| − | <th class="thHec">Part number</th>
| + | |
| − | <th class="thHec">Plate</th>
| + | |
| − | <th class="thHec">Location</th>
| + | |
| − | </tr>
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">Negative control</td>
| + | |
| − | <td class="tdHec">BBa_R0040</td>
| + | |
| − | <td class="tdHec">Kit Plate 7</td>
| + | |
| − | <td class="tdHec">Well 2D</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">Positive control</td>
| + | |
| − | <td class="tdHec">BBa_I20270</td>
| + | |
| − | <td class="tdHec">Kit Plate 7</td>
| + | |
| − | <td class="tdHec">Well 2B</td>
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Test Device 1</td>
| + | |
| − | <td class="tdHec">BBa_J364000</td>
| + | |
| − | <td class="tdHec">Kit Plate 7</td>
| + | |
| − | <td class="tdHec">Well 2F</td>
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Test Device 2</td>
| + | |
| − | <td class="tdHec">BBa_J364001</td>
| + | |
| − | <td class="tdHec">Kit Plate 7</td>
| + | |
| − | <td class="tdHec">Well 2H</td>
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Test Device 3</td>
| + | |
| − | <td class="tdHec">BBa_J364002</td>
| + | |
| − | <td class="tdHec">Kit Plate 7</td>
| + | |
| − | <td class="tdHec">Well 2J</td>
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Test Device 4</td>
| + | |
| − | <td class="tdHec">BBa_J364007</td>
| + | |
| − | <td class="tdHec">Kit Plate 7</td>
| + | |
| − | <td class="tdHec">Well 2L</td>
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Test Device 5</td>
| + | |
| − | <td class="tdHec">BBa_J364008</td>
| + | |
| − | <td class="tdHec">Kit Plate 7</td>
| + | |
| − | <td class="tdHec">Well 2N</td>
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Test Device 6</td>
| + | |
| − | <td class="tdHec">BBa_J364009</td>
| + | |
| − | <td class="tdHec">Kit Plate 7</td>
| + | |
| − | <td class="tdHec">Well 2P</td>
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <h4 style="
| + | |
| − | color: #353535;
| + | |
| − | font-weight: 700;
| + | |
| − | font-size: 21px;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | ">CALIBRATION 1: OD600 REFERENCE POINT</h4>
| + | |
| − | <p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> Using LUDOX CL-X as a point reference to obtain a conversion factor to transform our absorbance (Abs600) data from our plate reader into a comparable OD 600 measurement as would be obtained in a spectrophotometer.
| + | |
| − | | + | |
| − | </p>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec"></th>
| + | |
| − | <th class="thHec">LUDOX CL-X</th>
| + | |
| − | <th class="thHec">H2O</th>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">R1</td>
| + | |
| − | <td class="tdHec">0.061</td>
| + | |
| − | <td class="tdHec">0.051</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">R2</td>
| + | |
| − | <td class="tdHec">0.060</td>
| + | |
| − | <td class="tdHec">0.049</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">R3</td>
| + | |
| − | <td class="tdHec">0.061</td>
| + | |
| − | <td class="tdHec">0.043</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">R4</td>
| + | |
| − | <td class="tdHec">0.062</td>
| + | |
| − | <td class="tdHec">0.049</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Arith. Mean</td>
| + | |
| − | <td class="tdHec">0.061</td>
| + | |
| − | <td class="tdHec">0.048</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Corrected Abs600</td>
| + | |
| − | <td class="tdHec">0.013</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Reference OD600</td>
| + | |
| − | <td class="tdHec">0.063
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">OD600/Abs600</td>
| + | |
| − | <td class="tdHec">4.846</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <h4 style="
| + | |
| − | color: #353535;
| + | |
| − | font-weight: 700;
| + | |
| − | font-size: 21px;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | ">CALIBRATION 2: PARTICLE STANDARD CURVE</h4>
| + | |
| − | <p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> This allows us to construct a standard curve of particle concentration which can be used to convert Abs 600 measurements to an estimated number of cells.
| + | |
| − | | + | |
| − | <div style="
| + | |
| − | padding-bottom: 0.8em;
| + | |
| − | text-align: right;
| + | |
| − | ">
| + | |
| − | <div class="row" style="
| + | |
| − | margin: 0;
| + | |
| − | ">
| + | |
| − | <div class="col-md-6" style="
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| − | padding: 0;
| + | |
| − | padding-right: 0.3em;
| + | |
| − | ">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/3/3f/T--Valencia_UPV--im1UPV2018.jpeg" alt="" style="
| + | |
| − | margin-top: 1.8em;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | border-radius: 0.3em;
| + | |
| − | ">
| + | |
| − | </div>
| + | |
| − | <div class="col-md-6" style="
| + | |
| − | padding: 0;
| + | |
| − | padding-left: 0.3em;
| + | |
| − | ">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/2/28/T--Valencia_UPV--im2UPV2018.jpeg" alt="" style="
| + | |
| − | margin-top: 1.8em;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | border-radius: 0.3em;
| + | |
| − | ">
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | <h4 style="
| + | |
| − | color: #353535;
| + | |
| − | font-weight: 700;
| + | |
| − | font-size: 21px;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | ">CALIBRATION 3: FLUORESCENCE STANDARD CURVE</h4>
| + | |
| − | <p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> Absolute fluorescence values cannot be directly compared from one instrument to another. In order to compare fluorescence output of test devices between teams, it is necessary for each team to create a standard fluorescence curve.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | <div style="
| + | |
| − | padding-bottom: 0.8em;
| + | |
| − | text-align: right;
| + | |
| − | ">
| + | |
| − | <div class="row" style="
| + | |
| − | margin: 0;
| + | |
| − | ">
| + | |
| − | <div class="col-md-6" style="
| + | |
| − | padding: 0;
| + | |
| − | padding-right: 0.3em;
| + | |
| − | ">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/2/2e/T--Valencia_UPV--im3UPV2018.jpeg" alt="" style="
| + | |
| − | margin-top: 1.8em;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | border-radius: 0.3em;
| + | |
| − | ">
| + | |
| − | </div>
| + | |
| − | <div class="col-md-6" style="
| + | |
| − | padding: 0;
| + | |
| − | padding-left: 0.3em;
| + | |
| − | ">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/2/23/T--Valencia_UPV--im4UPV2018.jpeg" alt="" style="
| + | |
| − | margin-top: 1.8em;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | border-radius: 0.3em;
| + | |
| − | ">
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <h4 style="
| + | |
| − | color: #353535;
| + | |
| − | font-weight: 700;
| + | |
| − | font-size: 21px;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | ">EXPERIMENT</h4>
| + | |
| − | <h5 style="
| + | |
| − | color: #353535;
| + | |
| − | font-size: 16px;
| + | |
| − | font-weight: 750;
| + | |
| − | ">Fluorescence Raw Readings:</h5>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 0:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | <th class="thHec">LB+Chlor (blank)</th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">3618</td>
| + | |
| − | <td class="tdHec">3087</td>
| + | |
| − | <td class="tdHec">5355</td>
| + | |
| − | <td class="tdHec">2950</td>
| + | |
| − | <td class="tdHec">3402</td>
| + | |
| − | <td class="tdHec">4799</td>
| + | |
| − | <td class="tdHec">4112</td>
| + | |
| − | <td class="tdHec">3901</td>
| + | |
| − | <td class="tdHec">3350</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">3648</td>
| + | |
| − | <td class="tdHec">3414</td>
| + | |
| − | <td class="tdHec">5158</td>
| + | |
| − | <td class="tdHec">2887</td>
| + | |
| − | <td class="tdHec">3260</td>
| + | |
| − | <td class="tdHec">4647</td>
| + | |
| − | <td class="tdHec">4159</td>
| + | |
| − | <td class="tdHec">3758</td>
| + | |
| − | <td class="tdHec">3193</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">3273</td>
| + | |
| − | <td class="tdHec">3442</td>
| + | |
| − | <td class="tdHec">5077</td>
| + | |
| − | <td class="tdHec">1611</td>
| + | |
| − | <td class="tdHec">3331</td>
| + | |
| − | <td class="tdHec">4607</td>
| + | |
| − | <td class="tdHec">3972</td>
| + | |
| − | <td class="tdHec">3793</td>
| + | |
| − | <td class="tdHec">3234</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">3301</td>
| + | |
| − | <td class="tdHec">1381</td>
| + | |
| − | <td class="tdHec">5307</td>
| + | |
| − | <td class="tdHec">1074</td>
| + | |
| − | <td class="tdHec">3446</td>
| + | |
| − | <td class="tdHec">4519</td>
| + | |
| − | <td class="tdHec">4416</td>
| + | |
| − | <td class="tdHec">3804</td>
| + | |
| − | <td class="tdHec">3221</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">3350</td>
| + | |
| − | <td class="tdHec">3537</td>
| + | |
| − | <td class="tdHec">5091</td>
| + | |
| − | <td class="tdHec">3702</td>
| + | |
| − | <td class="tdHec">3976</td>
| + | |
| − | <td class="tdHec">4621</td>
| + | |
| − | <td class="tdHec">4517</td>
| + | |
| − | <td class="tdHec">3969</td>
| + | |
| − | <td class="tdHec">3256</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">3262</td>
| + | |
| − | <td class="tdHec">3409</td>
| + | |
| − | <td class="tdHec">4758</td>
| + | |
| − | <td class="tdHec">3623</td>
| + | |
| − | <td class="tdHec">3660</td>
| + | |
| − | <td class="tdHec">4208</td>
| + | |
| − | <td class="tdHec">4679</td>
| + | |
| − | <td class="tdHec">3867</td>
| + | |
| − | <td class="tdHec">3265</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">3255</td>
| + | |
| − | <td class="tdHec">3401</td>
| + | |
| − | <td class="tdHec">4784</td>
| + | |
| − | <td class="tdHec">3620</td>
| + | |
| − | <td class="tdHec">3662</td>
| + | |
| − | <td class="tdHec">4248</td>
| + | |
| − | <td class="tdHec">4481</td>
| + | |
| − | <td class="tdHec">3951</td>
| + | |
| − | <td class="tdHec">3231</td>
| + | |
| − |
| + | |
| − | </tr> <tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">3225</td>
| + | |
| − | <td class="tdHec">3020</td>
| + | |
| − | <td class="tdHec">4855</td>
| + | |
| − | <td class="tdHec">3518</td>
| + | |
| − | <td class="tdHec">3672</td>
| + | |
| − | <td class="tdHec">4451</td>
| + | |
| − | <td class="tdHec">4281</td>
| + | |
| − | <td class="tdHec">4183</td>
| + | |
| − | <td class="tdHec">4070</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 6:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | <th class="thHec">LB+Chlor (blank)</th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">4087</td>
| + | |
| − | <td class="tdHec">23103</td>
| + | |
| − | <td class="tdHec">44980</td>
| + | |
| − | <td class="tdHec">7751</td>
| + | |
| − | <td class="tdHec">4312</td>
| + | |
| − | <td class="tdHec">29208</td>
| + | |
| − | <td class="tdHec">6183</td>
| + | |
| − | <td class="tdHec">10720</td>
| + | |
| − | <td class="tdHec">3306</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">4238</td>
| + | |
| − | <td class="tdHec">23435</td>
| + | |
| − | <td class="tdHec">46319</td>
| + | |
| − | <td class="tdHec">7594</td>
| + | |
| − | <td class="tdHec">4341</td>
| + | |
| − | <td class="tdHec">28505</td>
| + | |
| − | <td class="tdHec">6522</td>
| + | |
| − | <td class="tdHec">10895</td>
| + | |
| − | <td class="tdHec">3177</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">4194</td>
| + | |
| − | <td class="tdHec">23871</td>
| + | |
| − | <td class="tdHec">45601</td>
| + | |
| − | <td class="tdHec">7757</td>
| + | |
| − | <td class="tdHec">4371</td>
| + | |
| − | <td class="tdHec">28765</td>
| + | |
| − | <td class="tdHec">6359</td>
| + | |
| − | <td class="tdHec">10515</td>
| + | |
| − | <td class="tdHec">3260</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">4195</td>
| + | |
| − | <td class="tdHec">24568</td>
| + | |
| − | <td class="tdHec">45744</td>
| + | |
| − | <td class="tdHec">7709</td>
| + | |
| − | <td class="tdHec">4546</td>
| + | |
| − | <td class="tdHec">29372</td>
| + | |
| − | <td class="tdHec">6156</td>
| + | |
| − | <td class="tdHec">11334</td>
| + | |
| − | <td class="tdHec">3205</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">4398</td>
| + | |
| − | <td class="tdHec">11707</td>
| + | |
| − | <td class="tdHec">43194</td>
| + | |
| − | <td class="tdHec">17428</td>
| + | |
| − | <td class="tdHec">4756</td>
| + | |
| − | <td class="tdHec">28009</td>
| + | |
| − | <td class="tdHec">11866</td>
| + | |
| − | <td class="tdHec">10612</td>
| + | |
| − | <td class="tdHec">3269</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">4351</td>
| + | |
| − | <td class="tdHec">12443</td>
| + | |
| − | <td class="tdHec">43496</td>
| + | |
| − | <td class="tdHec">17570</td>
| + | |
| − | <td class="tdHec">4804</td>
| + | |
| − | <td class="tdHec">28545</td>
| + | |
| − | <td class="tdHec">11928</td>
| + | |
| − | <td class="tdHec">10545</td>
| + | |
| − | <td class="tdHec">3250</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">4291</td>
| + | |
| − | <td class="tdHec">11451</td>
| + | |
| − | <td class="tdHec">43156</td>
| + | |
| − | <td class="tdHec">17421</td>
| + | |
| − | <td class="tdHec">4796</td>
| + | |
| − | <td class="tdHec">28475</td>
| + | |
| − | <td class="tdHec">11726</td>
| + | |
| − | <td class="tdHec">10544</td>
| + | |
| − | <td class="tdHec">3295</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">4292</td>
| + | |
| − | <td class="tdHec">12396</td>
| + | |
| − | <td class="tdHec">42704</td>
| + | |
| − | <td class="tdHec">17353</td>
| + | |
| − | <td class="tdHec">4938</td>
| + | |
| − | <td class="tdHec">28869</td>
| + | |
| − | <td class="tdHec">11517</td>
| + | |
| − | <td class="tdHec">10546</td>
| + | |
| − | <td class="tdHec">3231</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <h5 style="
| + | |
| − | color: #353535;
| + | |
| − | font-size: 16px;
| + | |
| − | font-weight: 750;
| + | |
| − | ">Abs600 Raw Readings:</h5>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 0:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | <th class="thHec">LB+Chlor (blank)</th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">0.067</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.060</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.047</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">0.061</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.054</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.046</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.047</td>
| + | |
| − | <td class="tdHec">0.065</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.045</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">0.062</td>
| + | |
| − | <td class="tdHec">0.047</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.048</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.045</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.046</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.054</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.054</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.047</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">0.071</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.071</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.046</td>
| + | |
| − |
| + | |
| − | </tr> <tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.049</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 6:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | <th class="thHec">LB+Chlor (blank)</th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">0.595</td>
| + | |
| − | <td class="tdHec">0.513</td>
| + | |
| − | <td class="tdHec">0.471</td>
| + | |
| − | <td class="tdHec">0.391</td>
| + | |
| − | <td class="tdHec">0.554</td>
| + | |
| − | <td class="tdHec">0.518</td>
| + | |
| − | <td class="tdHec">0.083</td>
| + | |
| − | <td class="tdHec">0.487</td>
| + | |
| − | <td class="tdHec">0.066</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">0.567</td>
| + | |
| − | <td class="tdHec">0.527</td>
| + | |
| − | <td class="tdHec">0.508</td>
| + | |
| − | <td class="tdHec">0.363</td>
| + | |
| − | <td class="tdHec">0.556</td>
| + | |
| − | <td class="tdHec">0.519</td>
| + | |
| − | <td class="tdHec">0.084</td>
| + | |
| − | <td class="tdHec">0.501</td>
| + | |
| − | <td class="tdHec">0.053</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">0.566</td>
| + | |
| − | <td class="tdHec">0.524</td>
| + | |
| − | <td class="tdHec">0.479</td>
| + | |
| − | <td class="tdHec">0.381</td>
| + | |
| − | <td class="tdHec">0.566</td>
| + | |
| − | <td class="tdHec">0.501</td>
| + | |
| − | <td class="tdHec">0.083</td>
| + | |
| − | <td class="tdHec">0.490</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">0.580</td>
| + | |
| − | <td class="tdHec">0.570</td>
| + | |
| − | <td class="tdHec">0.530</td>
| + | |
| − | <td class="tdHec">0.373</td>
| + | |
| − | <td class="tdHec">0.549</td>
| + | |
| − | <td class="tdHec">0.559</td>
| + | |
| − | <td class="tdHec">0.088</td>
| + | |
| − | <td class="tdHec">0.551</td>
| + | |
| − | <td class="tdHec">0.051</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">0.611</td>
| + | |
| − | <td class="tdHec">0.513</td>
| + | |
| − | <td class="tdHec">0.519</td>
| + | |
| − | <td class="tdHec">0.531</td>
| + | |
| − | <td class="tdHec">0.515</td>
| + | |
| − | <td class="tdHec">0.530</td>
| + | |
| − | <td class="tdHec">0.117</td>
| + | |
| − | <td class="tdHec">0.520</td>
| + | |
| − | <td class="tdHec">0.053</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">0.562</td>
| + | |
| − | <td class="tdHec">0.538</td>
| + | |
| − | <td class="tdHec">0.523</td>
| + | |
| − | <td class="tdHec">0.536</td>
| + | |
| − | <td class="tdHec">0.522</td>
| + | |
| − | <td class="tdHec">0.543</td>
| + | |
| − | <td class="tdHec">0.116</td>
| + | |
| − | <td class="tdHec">0.517</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">0.561</td>
| + | |
| − | <td class="tdHec">0.493</td>
| + | |
| − | <td class="tdHec">0.517</td>
| + | |
| − | <td class="tdHec">0.507</td>
| + | |
| − | <td class="tdHec">0.518</td>
| + | |
| − | <td class="tdHec">0.502</td>
| + | |
| − | <td class="tdHec">0.106</td>
| + | |
| − | <td class="tdHec">0.488</td>
| + | |
| − | <td class="tdHec">0.054</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">0.555</td>
| + | |
| − | <td class="tdHec">0.537</td>
| + | |
| − | <td class="tdHec">0.498</td>
| + | |
| − | <td class="tdHec">0.512</td>
| + | |
| − | <td class="tdHec">0.533</td>
| + | |
| − | <td class="tdHec">0.508</td>
| + | |
| − | <td class="tdHec">0.101</td>
| + | |
| − | <td class="tdHec">0.491</td>
| + | |
| − | <td class="tdHec">0.06</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <h5 style="
| + | |
| − | color: #353535;
| + | |
| − | font-size: 16px;
| + | |
| − | font-weight: 750;
| + | |
| − | ">uM Fluorescein/OD:</h5>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 0:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | </th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">0.415</td>
| + | |
| − | <td class="tdHec">-1.019</td>
| + | |
| − | <td class="tdHec">4.780</td>
| + | |
| − | <td class="tdHec">-1.377</td>
| + | |
| − | <td class="tdHec">0.134</td>
| + | |
| − | <td class="tdHec">3.742</td>
| + | |
| − | <td class="tdHec">2.362</td>
| + | |
| − | <td class="tdHec">1.423</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">0.940</td>
| + | |
| − | <td class="tdHec">0.685</td>
| + | |
| − | <td class="tdHec">5.075</td>
| + | |
| − | <td class="tdHec">-1.185</td>
| + | |
| − | <td class="tdHec">0.160</td>
| + | |
| − | <td class="tdHec">4.506</td>
| + | |
| − | <td class="tdHec">2.495</td>
| + | |
| − | <td class="tdHec">1.459</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">0.101</td>
| + | |
| − | <td class="tdHec">0.496</td>
| + | |
| − | <td class="tdHec">4.760</td>
| + | |
| − | <td class="tdHec">-25.149</td>
| + | |
| − | <td class="tdHec">0.150</td>
| + | |
| − | <td class="tdHec">3.273</td>
| + | |
| − | <td class="tdHec">2.079</td>
| + | |
| − | <td class="tdHec">1.237</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">0.155</td>
| + | |
| − | <td class="tdHec">-28.512</td>
| + | |
| − | <td class="tdHec">4.973</td>
| + | |
| − | <td class="tdHec">-22.179</td>
| + | |
| − | <td class="tdHec">0.536</td>
| + | |
| − | <td class="tdHec">3.352</td>
| + | |
| − | <td class="tdHec">3.086</td>
| + | |
| − | <td class="tdHec">1.291</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">0.324</td>
| + | |
| − | <td class="tdHec">0.968</td>
| + | |
| − | <td class="tdHec">4.739</td>
| + | |
| − | <td class="tdHec">1.536</td>
| + | |
| − | <td class="tdHec">1.716</td>
| + | |
| − | <td class="tdHec">4.700</td>
| + | |
| − | <td class="tdHec">3.908</td>
| + | |
| − | <td class="tdHec">2.210</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">-0.012</td>
| + | |
| − | <td class="tdHec">0.372</td>
| + | |
| − | <td class="tdHec">6.610</td>
| + | |
| − | <td class="tdHec">1.233</td>
| + | |
| − | <td class="tdHec">1.224</td>
| + | |
| − | <td class="tdHec">4.175</td>
| + | |
| − | <td class="tdHec">3.652</td>
| + | |
| − | <td class="tdHec">1.696</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">0.030</td>
| + | |
| − | <td class="tdHec">0.585</td>
| + | |
| − | <td class="tdHec">4.813</td>
| + | |
| − | <td class="tdHec">0.482</td>
| + | |
| − | <td class="tdHec">1.336</td>
| + | |
| − | <td class="tdHec">2.865</td>
| + | |
| − | <td class="tdHec">3.522</td>
| + | |
| − | <td class="tdHec">1.859</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr> <tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">-3.741</td>
| + | |
| − | <td class="tdHec">-3.254</td>
| + | |
| − | <td class="tdHec">4.055</td>
| + | |
| − | <td class="tdHec">-2.138</td>
| + | |
| − | <td class="tdHec">-2.056</td>
| + | |
| − | <td class="tdHec">1.581</td>
| + | |
| − | <td class="tdHec">0.934</td>
| + | |
| − | <td class="tdHec">0.350</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 6:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | </tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">0.046</td>
| + | |
| − | <td class="tdHec">1.373</td>
| + | |
| − | <td class="tdHec">3.189</td>
| + | |
| − | <td class="tdHec">0.424</td>
| + | |
| − | <td class="tdHec">0.064</td>
| + | |
| − | <td class="tdHec">1.776</td>
| + | |
| − | <td class="tdHec">5.245</td>
| + | |
| − | <td class="tdHec">0.546</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">0.064</td>
| + | |
| − | <td class="tdHec">1.325</td>
| + | |
| − | <td class="tdHec">2.939</td>
| + | |
| − | <td class="tdHec">0.442</td>
| + | |
| − | <td class="tdHec">0.072</td>
| + | |
| − | <td class="tdHec">1.684</td>
| + | |
| − | <td class="tdHec">3.344</td>
| + | |
| − | <td class="tdHec">0.534</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">1.374</td>
| + | |
| − | <td class="tdHec">3.124</td>
| + | |
| − | <td class="tdHec">0.433</td>
| + | |
| − | <td class="tdHec">0.068</td>
| + | |
| − | <td class="tdHec">1.788</td>
| + | |
| − | <td class="tdHec">4.002</td>
| + | |
| − | <td class="tdHec">0.522</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">1.276</td>
| + | |
| − | <td class="tdHec">2.752</td>
| + | |
| − | <td class="tdHec">0.433</td>
| + | |
| − | <td class="tdHec">0.083</td>
| + | |
| − | <td class="tdHec">1.596</td>
| + | |
| − | <td class="tdHec">2.472</td>
| + | |
| − | <td class="tdHec">0.504</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">0.063</td>
| + | |
| − | <td class="tdHec">0.568</td>
| + | |
| − | <td class="tdHec">2.655</td>
| + | |
| − | <td class="tdHec">0.918</td>
| + | |
| − | <td class="tdHec">0.100</td>
| + | |
| − | <td class="tdHec">1.607</td>
| + | |
| − | <td class="tdHec">4.163</td>
| + | |
| − | <td class="tdHec">0.487</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">0.068</td>
| + | |
| − | <td class="tdHec">0.592</td>
| + | |
| − | <td class="tdHec">2.677</td>
| + | |
| − | <td class="tdHec">0.927</td>
| + | |
| − | <td class="tdHec">0.104</td>
| + | |
| − | <td class="tdHec">1.613</td>
| + | |
| − | <td class="tdHec">4.558</td>
| + | |
| − | <td class="tdHec">0.491</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">0.061</td>
| + | |
| − | <td class="tdHec">0.576</td>
| + | |
| − | <td class="tdHec">2.668</td>
| + | |
| − | <td class="tdHec">0.966</td>
| + | |
| − | <td class="tdHec">0.100</td>
| + | |
| − | <td class="tdHec">1.742</td>
| + | |
| − | <td class="tdHec">5.025</td>
| + | |
| − | <td class="tdHec">0.518</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">0.066</td>
| + | |
| − | <td class="tdHec">0.595</td>
| + | |
| − | <td class="tdHec">2.793</td>
| + | |
| − | <td class="tdHec">0.968</td>
| + | |
| − | <td class="tdHec">0.112</td>
| + | |
| − | <td class="tdHec">1.764</td>
| + | |
| − | <td class="tdHec">6.263</td>
| + | |
| − | <td class="tdHec">0.526</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <h5 style="
| + | |
| − | color: #353535;
| + | |
| − | font-size: 16px;
| + | |
| − | font-weight: 750;
| + | |
| − | ">MEFL/particle:</h5>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 0:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | </th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">1.31E+05</td>
| + | |
| − | <td class="tdHec">-3.22E+05</td>
| + | |
| − | <td class="tdHec">1.51E+06</td>
| + | |
| − | <td class="tdHec">-4.35E+05</td>
| + | |
| − | <td class="tdHec">4.24E+04</td>
| + | |
| − | <td class="tdHec">1.18E+06</td>
| + | |
| − | <td class="tdHec">7.46E+05</td>
| + | |
| − | <td class="tdHec">4.50E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">2.97E+05</td>
| + | |
| − | <td class="tdHec">2.16E+05</td>
| + | |
| − | <td class="tdHec">1.60E+06</td>
| + | |
| − | <td class="tdHec">-3.75E+05</td>
| + | |
| − | <td class="tdHec">5.05E+04</td>
| + | |
| − | <td class="tdHec">1.42E+06</td>
| + | |
| − | <td class="tdHec">7.89E+05</td>
| + | |
| − | <td class="tdHec">4.61E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">3.18E+04</td>
| + | |
| − | <td class="tdHec">1.57E+05</td>
| + | |
| − | <td class="tdHec">1.50E+06</td>
| + | |
| − | <td class="tdHec">-7.95E+06</td>
| + | |
| − | <td class="tdHec">4.75E+04</td>
| + | |
| − | <td class="tdHec">1.03E+06</td>
| + | |
| − | <td class="tdHec">6.57E+05</td>
| + | |
| − | <td class="tdHec">3.91E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">4.90E+04</td>
| + | |
| − | <td class="tdHec">-9011826</td>
| + | |
| − | <td class="tdHec">1.57E+06</td>
| + | |
| − | <td class="tdHec">-7.01E+06</td>
| + | |
| − | <td class="tdHec">1.70E+05</td>
| + | |
| − | <td class="tdHec">1.06E+06</td>
| + | |
| − | <td class="tdHec">9.75E+05</td>
| + | |
| − | <td class="tdHec">4.08E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">1.02E+05</td>
| + | |
| − | <td class="tdHec">3.06E+05</td>
| + | |
| − | <td class="tdHec">1.50E+06</td>
| + | |
| − | <td class="tdHec">4.85E+05</td>
| + | |
| − | <td class="tdHec">5.43E+05</td>
| + | |
| − | <td class="tdHec">1.49E+06</td>
| + | |
| − | <td class="tdHec">1.24E+06</td>
| + | |
| − | <td class="tdHec">6.98E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">-3.67E+03</td>
| + | |
| − | <td class="tdHec">1.18E+05</td>
| + | |
| − | <td class="tdHec">2.09E+06</td>
| + | |
| − | <td class="tdHec">3.90E+05</td>
| + | |
| − | <td class="tdHec">3.87E+05</td>
| + | |
| − | <td class="tdHec">1.32E+06</td>
| + | |
| − | <td class="tdHec">1.15E+06</td>
| + | |
| − | <td class="tdHec">5.36E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">9.40E+03</td>
| + | |
| − | <td class="tdHec">1.85E+05</td>
| + | |
| − | <td class="tdHec">2.09E+06</td>
| + | |
| − | <td class="tdHec">1.52E+05</td>
| + | |
| − | <td class="tdHec">4.22E+05</td>
| + | |
| − | <td class="tdHec">9.06E+05</td>
| + | |
| − | <td class="tdHec">1.11E+06</td>
| + | |
| − | <td class="tdHec">5.88E+05</td>
| + | |
| − |
| + | |
| − | </tr> <tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">-1.18E+06</td>
| + | |
| − | <td class="tdHec">-1.03E+06</td>
| + | |
| − | <td class="tdHec">1.28E+06</td>
| + | |
| − | <td class="tdHec">-6.76E+05</td>
| + | |
| − | <td class="tdHec">-6.50E+05</td>
| + | |
| − | <td class="tdHec">3.73E+05</td>
| + | |
| − | <td class="tdHec">2.95E+05</td>
| + | |
| − | <td class="tdHec">1.11E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 6:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | </tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">1.45E+04</td>
| + | |
| − | <td class="tdHec">4.34E+05</td>
| + | |
| − | <td class="tdHec">1.01E+06</td>
| + | |
| − | <td class="tdHec">1.34E+05</td>
| + | |
| − | <td class="tdHec">2.02E+04</td>
| + | |
| − | <td class="tdHec">5.61E+05</td>
| + | |
| − | <td class="tdHec">1.66E+06</td>
| + | |
| − | <td class="tdHec">1.73E+05</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">2.02E+04</td>
| + | |
| − | <td class="tdHec">4.19E+05</td>
| + | |
| − | <td class="tdHec">9.29E+05</td>
| + | |
| − | <td class="tdHec">1.40E+05</td>
| + | |
| − | <td class="tdHec">2.27E+04</td>
| + | |
| − | <td class="tdHec">5.32E+05</td>
| + | |
| − | <td class="tdHec">1.06E+06</td>
| + | |
| − | <td class="tdHec">1.69E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">1.80E+04</td>
| + | |
| − | <td class="tdHec">4.34E+05</td>
| + | |
| − | <td class="tdHec">9.87E+05</td>
| + | |
| − | <td class="tdHec">1.37E+05</td>
| + | |
| − | <td class="tdHec">2.15E+04</td>
| + | |
| − | <td class="tdHec">5.65E+05</td>
| + | |
| − | <td class="tdHec">1.26E+06</td>
| + | |
| − | <td class="tdHec">1.65E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">1.83E+04</td>
| + | |
| − | <td class="tdHec">4.03E+05</td>
| + | |
| − | <td class="tdHec">8.70E+05</td>
| + | |
| − | <td class="tdHec">1.37E+05</td>
| + | |
| − | <td class="tdHec">2.64E+04</td>
| + | |
| − | <td class="tdHec">5.05E+05</td>
| + | |
| − | <td class="tdHec">7.81E+05</td>
| + | |
| − | <td class="tdHec">1.59E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">1.98E+04</td>
| + | |
| − | <td class="tdHec">1.80E+05</td>
| + | |
| − | <td class="tdHec">8.39E+05</td>
| + | |
| − | <td class="tdHec">2.90E+05</td>
| + | |
| − | <td class="tdHec">3.15E+04</td>
| + | |
| − | <td class="tdHec">5.08E+05</td>
| + | |
| − | <td class="tdHec">1.32E+06</td>
| + | |
| − | <td class="tdHec">1.54E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">2.14E+04</td>
| + | |
| − | <td class="tdHec">1.87E+05</td>
| + | |
| − | <td class="tdHec">8.46E+05</td>
| + | |
| − | <td class="tdHec">2.93E+05</td>
| + | |
| − | <td class="tdHec">3.27E+04</td>
| + | |
| − | <td class="tdHec">5.10E+05</td>
| + | |
| − | <td class="tdHec">1.44E+06</td>
| + | |
| − | <td class="tdHec">1.55E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">1.92E+04</td>
| + | |
| − | <td class="tdHec">1.82E+05</td>
| + | |
| − | <td class="tdHec">8.43E+0.5</td>
| + | |
| − | <td class="tdHec">3.05E+05</td>
| + | |
| − | <td class="tdHec">3.17E+04</td>
| + | |
| − | <td class="tdHec">5.51E+05</td>
| + | |
| − | <td class="tdHec">1.59E+06</td>
| + | |
| − | <td class="tdHec">1.64E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">2.10E+04</td>
| + | |
| − | <td class="tdHec">1.88E+05</td>
| + | |
| − | <td class="tdHec">8.83E+05</td>
| + | |
| − | <td class="tdHec">3.06E+05</td>
| + | |
| − | <td class="tdHec">3.54E+04</td>
| + | |
| − | <td class="tdHec">5.61E+05</td>
| + | |
| − | <td class="tdHec">1.98E+06</td>
| + | |
| − | <td class="tdHec">1.66E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| | | | |
| | + | <p>We are continuously talking about a machine which can create its <b>own genetic circuits</b>, by using pre-designed parts, and ‘print’ them inside different living cell chassis. But how is Printeria going to perform all these complex reactions?</p> |
| | | | |
| | | | |
