Difference between revisions of "Team:Aalto-Helsinki/Demonstrate"
| Line 8: | Line 8: | ||
<br> | <br> | ||
| − | <h1 align="center"> | + | <h1 align="center">Results</h1> |
<br> | <br> | ||
Revision as of 06:45, 12 October 2018
Results
Cloning
We assembled our constructs and cloned them into pSVA1 plasmid using Golden Gate assembly. After transforming plasmids to E.Coli Beta-10 and plasmid isolation we performed a check-up digestion with restriction enzymes NcoI and XhoI which cut the plasmid backbone close to BsaI insert site.
Protein expression and purification
We were able to successfully purify our silk containing construct prancerPurple-silk-CBM with the heat protocol
Cellulose binding
Eget duis at tellus at urna. Lacinia quis vel eros donec. Ut pharetra sit amet aliquam id diam. Erat imperdiet sed euismod nisi porta.
Cellulose nanofibril binding
Eget duis at tellus at urna. Lacinia quis vel eros donec. Ut pharetra sit amet aliquam id diam. Erat imperdiet sed euismod nisi porta.
Cotton binding
Eget duis at tellus at urna. Lacinia quis vel eros donec. Ut pharetra sit amet aliquam id diam. Erat imperdiet sed euismod nisi porta.
Binding to hair keratin
Eget duis at tellus at urna. Lacinia quis vel eros donec. Ut pharetra sit amet aliquam id diam. Erat imperdiet sed euismod nisi porta.