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Revision as of 07:22, 12 October 2018

Interlab with ZJUT-China

    This year is our first time to attend for the Igem, So We met up with ZJUT-China,DLUT-China and LZU-China in Jiangsu Normal University to learn some experience form them and share our own project to them . Before this meeting, we had some troubles in interlab and we couldn’t find correct method to solve it. So we shared our problems with them at the meeting. The ZJUT-China had finished the interlab successfully and gave us some suggestions on our interlab. We adopted their suggestions and finally made it. After this meeting, we had a further communication with ZJUT-China and What they studied in Lysis gene gave us some inspiration about our own project, which made us consider to add a kill switch to make our L.lactis safer towards the biological environment. In return, we also gave them some ideas and suggestions to help them with their project.


Kill switch with SSTi-SGZD

    We studied for a time before doing interlab, and spent one-two day preparing experimental materials, such as preparing E. coli DH5α and several culture media. After full preparation, we started the experiment. In the first experiment, our antibiotics failed, causing too much bacteria to colonize the plate.The second time we had a problem with the competent cells, so the bacteria did not grow after the plate was painted.The third time, we reprepared the competent cells and replaced the antibiotics, and the experiment went well.



Satety with Unesp_Brazilr

    This year we also contacted with the team Unesp_Brazilr by e-mails. We all concerned about the safety of the synthetic biology so we talked more about how to construct a high efficient chassis microorganism which is safe enough. Although we wanted to make our strain applied into the practical application, we didn’t know anything about the application of engineering bacteria. Fortunately, Unesp_Brazil offered the regulations and instructed us to apply our bacteria in the correct way. They also offered us the gene which could be added as the kill switch, helping us to notice the importance of using a kill switch to make our bacteria safer. Of course, we give some methods to help with their experiments and shared some experience about our project. Because this is the first time to attend for the IGEM, we are very appreciated that Unesp_Brazil could share experience with us and adopted our ideas even though we are a new team for IGEM.



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