Difference between revisions of "Team:Mingdao/Collaborations"

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{{:Team:Mingdao/test9}}
 
{{:Team:Mingdao/test9}}
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templated.co @templatedco
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            <h1 id = "d-overview">Experiment</h1>     
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<h1>Collaboration</h1>
  
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This year, we collaborated with a collegiate team NCHU-Taichung iGEM 2018 from National Chung Hsing University, Taiwan. NCHU is just located nearby Mingdao High School (15 minutes distance by car). At very beginning of the journey in iGEM 2018, we met up with each other at NCHU and Mingdao’s laboratory and school campus. We helped them host the 6th Asian-Pacific Conference hold in August. We shared with each other many ideas and dealt with problems met in the project and experiments. In this moment, just several weeks before iGEM Jamboree, we’d like to say, “Thank you, Friends. Happy to meet you because of iGEM” 
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We helped iGEM team NCHU to verify the bacterial cell growth affected by their BioBrick devices. They want to utilize synthetic biology to recover an environment polluted with dioxin. They created BioBricks of HAD, TonB and TLCC. They prepared the transformed E. coli with BioBricks and growth media (KB medium). Go to our lab in Mingdao, we measured the OD values of the E. coli cultured overnight and added IPTG for the induction of the gene expression and completed the assay following their protocol and instructions.
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+
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<br />
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<img class="center" src="https://static.igem.org/mediawiki/2018/3/3c/T--Mingdao--project_intro2.png" alt="" style="width: 20%; margin-bottom: 20px;">
 +
<img class="center" src="https://static.igem.org/mediawiki/2018/3/3c/T--Mingdao--project_intro2.png" alt="" style="width: 20%; margin-bottom: 20px;">
 +
<br />
 +
<h3>EXPERIMENT BY MINGDAO</h3>
 +
<p >
 +
Measure OD600 values for E. coli with HAD, TonB or TLCC genes<br />
 +
Dilute to OD600=0.05 with KB medium<br />
 +
Culture at for 2-3 hours until OD values reach approximately 0.6<br />
 +
Add 1mM IPTG for gene induction<br />
 +
Set a kinetic mode for analysis in Microplate Reader (BioTeK Synergy H1)<br />
 +
Analyze the data with each group for 3 repeats and report to Team NCHU  <br />
 +
</p>
 +
<img class="center" src="https://static.igem.org/mediawiki/2018/3/3c/T--Mingdao--project_intro2.png" alt="" style="width: 20%; margin-bottom: 20px;">
 +
<br />
 +
<h3>RESULT</h3>
 +
<p style="text-indent:2em">
 +
The figure showed TonB helped bacterial growth and further improved by HAD. However, TLCC seems some toxic effect on bacterial growth.
 +
</p>
 +
<br />
 +
<img class="center" src="https://static.igem.org/mediawiki/2018/1/1f/T--Mingdao--project_mos3.png" alt="" style="width: 50%; margin-bottom: 20px;">
 +
<p style="text-indent:2em">
 +
On the other hand, we want to demonstrate our pathogen testing system in real adult mosquitoes and understand the GFP reporter system driven by GAM1 promoter can be triggered by E. coli. We prepared the plasmids of GAM1-GFP-polyA /pSB1C3 and heat-killed E. coli. Team NCUH brought us to the Mosquito Lab at Entomology Department of National Chung Hsing University and helped us microinjected DNAs into the midgut of Aedes aegypti.
 +
</p>
 +
<br />
 +
<img class="center" src="https://static.igem.org/mediawiki/2018/1/1f/T--Mingdao--project_mos3.png" alt="" style="width: 50%; margin-bottom: 20px;">
 +
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 +
<br />
 +
<h3>EXPERIMENT BY NCHU</h3>
 +
<p >
 +
Make microinjection needles by pulling glass capillary tubes<br />
 +
Anesthetize the adult mosquitoes on ice<br />
 +
Fill the needle with the DNAs in Cellfectin II reagent with E. coli or not<br />
 +
Inject the DNA by inserting into the thorax of the mosquito.<br />
 +
Recover the mosquito for 2 days<br />
 +
</p>
 +
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 +
<br />
 +
<h3>RESULT</h3>
 +
<p style="text-indent:2em">
 +
The mosquito injected with DNA plus E. coli showed fluorescence signal in Gel Imaging System and Blue LED Box after 2 days of DNA microinjection.
 +
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 +
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          <p>Syringe</p>
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          <p>GFP System</p>
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          <p>Mosquito Immune Signaling</p>
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+
 
+
.container.\31 25\25 {
+
width: 100%;
+
max-width: 112.5%;
+
min-width: 90%;
+
}
+
 
+
.container.\37 5\25 {
+
width: 67.5%;
+
}
+
 
+
.container.\35 0\25 {
+
width: 45%;
+
}
+
 
+
.container.\32 5\25 {
+
width: 22.5%;
+
}
+
 
+
.container {
+
width: 90% !important;
+
}
+
 
+
}
+
 
+
/* Grid */
+
 
+
.row {
+
border-bottom: solid 1px transparent;
+
-moz-box-sizing: border-box;
+
-webkit-box-sizing: border-box;
+
box-sizing: border-box;
+
}
+
 
+
.row > * {
+
float: left;
+
-moz-box-sizing: border-box;
+
-webkit-box-sizing: border-box;
+
box-sizing: border-box;
+
}
+
 
+
.row:after, .row:before {
+
content: '';
+
display: block;
+
clear: both;
+
height: 0;
+
}
+
 
+
.row.uniform > * > :first-child {
+
margin-top: 0;
+
}
+
 
+
.row.uniform > * > :last-child {
+
margin-bottom: 0;
+
}
+
 
+
.row.\30 \25 > * {
+
padding: 0 0 0 0rem;
+
}
+
 
+
.row.\30 \25 {
+
margin: 0 0 -1px 0rem;
+
}
+
 
+
.row.uniform.\30 \25 > * {
+
padding: 0rem 0 0 0rem;
+
}
+
 
+
.row.uniform.\30 \25 {
+
margin: 0rem 0 -1px 0rem;
+
}
+
 
+
.row > * {
+
padding: 0 0 0 2rem;
+
}
+
 
+
.row {
+
margin: 0 0 -1px -2rem;
+
}
+
 
+
.row.uniform > * {
+
padding: 2rem 0 0 2rem;
+
}
+
 
+
.row.uniform {
+
margin: -2rem 0 -1px -2rem;
+
}
+
 
+
.row.\32 00\25 > * {
+
padding: 0 0 0 4rem;
+
}
+
 
+
.row.\32 00\25 {
+
margin: 0 0 -1px -4rem;
+
}
+
 
+
.row.uniform.\32 00\25 > * {
+
padding: 4rem 0 0 4rem;
+
}
+
 
+
.row.uniform.\32 00\25 {
+
margin: -4rem 0 -1px -4rem;
+
}
+
 
+
.row.\31 50\25 > * {
+
padding: 0 0 0 3rem;
+
}
+
 
+
.row.\31 50\25 {
+
margin: 0 0 -1px -3rem;
+
}
+
 
+
.row.uniform.\31 50\25 > * {
+
padding: 3rem 0 0 3rem;
+
}
+
 
+
.row.uniform.\31 50\25 {
+
margin: -3rem 0 -1px -3rem;
+
}
+
 
+
.row.\35 0\25 > * {
+
padding: 0 0 0 1rem;
+
}
+
 
+
.row.\35 0\25 {
+
margin: 0 0 -1px -1rem;
+
}
+
 
+
.row.uniform.\35 0\25 > * {
+
padding: 1rem 0 0 1rem;
+
}
+
 
+
.row.uniform.\35 0\25 {
+
margin: -1rem 0 -1px -1rem;
+
}
+
 
+
.row.\32 5\25 > * {
+
padding: 0 0 0 0.5rem;
+
}
+
 
+
.row.\32 5\25 {
+
margin: 0 0 -1px -0.5rem;
+
}
+
 
+
.row.uniform.\32 5\25 > * {
+
padding: 0.5rem 0 0 0.5rem;
+
}
+
 
+
.row.uniform.\32 5\25 {
+
margin: -0.5rem 0 -1px -0.5rem;
+
}
+
 
+
.\31 2u, .\31 2u\24 {
+
width: 100%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 1u, .\31 1u\24 {
+
width: 91.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 0u, .\31 0u\24 {
+
width: 83.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\39 u, .\39 u\24 {
+
width: 75%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\38 u, .\38 u\24 {
+
width: 66.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\37 u, .\37 u\24 {
+
width: 58.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\36 u, .\36 u\24 {
+
width: 50%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\35 u, .\35 u\24 {
+
width: 41.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\34 u, .\34 u\24 {
+
width: 33.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\33 u, .\33 u\24 {
+
width: 25%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\32 u, .\32 u\24 {
+
width: 16.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 u, .\31 u\24 {
+
width: 8.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 2u\24 + *,
+
.\31 1u\24 + *,
+
.\31 0u\24 + *,
+
.\39 u\24 + *,
+
.\38 u\24 + *,
+
.\37 u\24 + *,
+
.\36 u\24 + *,
+
.\35 u\24 + *,
+
.\34 u\24 + *,
+
.\33 u\24 + *,
+
.\32 u\24 + *,
+
.\31 u\24 + * {
+
clear: left;
+
}
+
 
+
.\-11u {
+
margin-left: 91.66667%;
+
}
+
 
+
.\-10u {
+
margin-left: 83.33333%;
+
}
+
 
+
.\-9u {
+
margin-left: 75%;
+
}
+
 
+
.\-8u {
+
margin-left: 66.66667%;
+
}
+
 
+
.\-7u {
+
margin-left: 58.33333%;
+
}
+
 
+
.\-6u {
+
margin-left: 50%;
+
}
+
 
+
.\-5u {
+
margin-left: 41.66667%;
+
}
+
 
+
.\-4u {
+
margin-left: 33.33333%;
+
}
+
 
+
.\-3u {
+
margin-left: 25%;
+
}
+
 
+
.\-2u {
+
margin-left: 16.66667%;
+
}
+
 
+
.\-1u {
+
margin-left: 8.33333%;
+
}
+
 
+
@media screen and (max-width: 1680px) {
+
 
+
.row > * {
+
padding: 0 0 0 2rem;
+
}
+
 
+
.row {
+
margin: 0 0 -1px -2rem;
+
}
+
 
+
.row.uniform > * {
+
padding: 2rem 0 0 2rem;
+
}
+
 
+
.row.uniform {
+
margin: -2rem 0 -1px -2rem;
+
}
+
 
+
.row.\32 00\25 > * {
+
padding: 0 0 0 4rem;
+
}
+
 
+
.row.\32 00\25 {
+
margin: 0 0 -1px -4rem;
+
}
+
 
+
.row.uniform.\32 00\25 > * {
+
padding: 4rem 0 0 4rem;
+
}
+
 
+
.row.uniform.\32 00\25 {
+
margin: -4rem 0 -1px -4rem;
+
}
+
 
+
.row.\31 50\25 > * {
+
padding: 0 0 0 3rem;
+
}
+
 
+
.row.\31 50\25 {
+
margin: 0 0 -1px -3rem;
+
}
+
 
+
.row.uniform.\31 50\25 > * {
+
padding: 3rem 0 0 3rem;
+
}
+
 
+
.row.uniform.\31 50\25 {
+
margin: -3rem 0 -1px -3rem;
+
}
+
 
+
.row.\35 0\25 > * {
+
padding: 0 0 0 1rem;
+
}
+
 
+
.row.\35 0\25 {
+
margin: 0 0 -1px -1rem;
+
}
+
 
+
.row.uniform.\35 0\25 > * {
+
padding: 1rem 0 0 1rem;
+
}
+
 
+
.row.uniform.\35 0\25 {
+
margin: -1rem 0 -1px -1rem;
+
}
+
 
+
.row.\32 5\25 > * {
+
padding: 0 0 0 0.5rem;
+
}
+
 
+
.row.\32 5\25 {
+
margin: 0 0 -1px -0.5rem;
+
}
+
 
+
.row.uniform.\32 5\25 > * {
+
padding: 0.5rem 0 0 0.5rem;
+
}
+
 
+
.row.uniform.\32 5\25 {
+
margin: -0.5rem 0 -1px -0.5rem;
+
}
+
 
+
.\31 2u\28xlarge\29, .\31 2u\24\28xlarge\29 {
+
width: 100%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 1u\28xlarge\29, .\31 1u\24\28xlarge\29 {
+
width: 91.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 0u\28xlarge\29, .\31 0u\24\28xlarge\29 {
+
width: 83.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\39 u\28xlarge\29, .\39 u\24\28xlarge\29 {
+
width: 75%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\38 u\28xlarge\29, .\38 u\24\28xlarge\29 {
+
width: 66.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\37 u\28xlarge\29, .\37 u\24\28xlarge\29 {
+
width: 58.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\36 u\28xlarge\29, .\36 u\24\28xlarge\29 {
+
width: 50%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\35 u\28xlarge\29, .\35 u\24\28xlarge\29 {
+
width: 41.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\34 u\28xlarge\29, .\34 u\24\28xlarge\29 {
+
width: 33.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\33 u\28xlarge\29, .\33 u\24\28xlarge\29 {
+
width: 25%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\32 u\28xlarge\29, .\32 u\24\28xlarge\29 {
+
width: 16.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 u\28xlarge\29, .\31 u\24\28xlarge\29 {
+
width: 8.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 2u\24\28xlarge\29 + *,
+
.\31 1u\24\28xlarge\29 + *,
+
.\31 0u\24\28xlarge\29 + *,
+
.\39 u\24\28xlarge\29 + *,
+
.\38 u\24\28xlarge\29 + *,
+
.\37 u\24\28xlarge\29 + *,
+
.\36 u\24\28xlarge\29 + *,
+
.\35 u\24\28xlarge\29 + *,
+
.\34 u\24\28xlarge\29 + *,
+
.\33 u\24\28xlarge\29 + *,
+
.\32 u\24\28xlarge\29 + *,
+
.\31 u\24\28xlarge\29 + * {
+
clear: left;
+
}
+
 
+
.\-11u\28xlarge\29 {
+
margin-left: 91.66667%;
+
}
+
 
+
.\-10u\28xlarge\29 {
+
margin-left: 83.33333%;
+
}
+
 
+
.\-9u\28xlarge\29 {
+
margin-left: 75%;
+
}
+
 
+
.\-8u\28xlarge\29 {
+
margin-left: 66.66667%;
+
}
+
 
+
.\-7u\28xlarge\29 {
+
margin-left: 58.33333%;
+
}
+
 
+
.\-6u\28xlarge\29 {
+
margin-left: 50%;
+
}
+
 
+
.\-5u\28xlarge\29 {
+
margin-left: 41.66667%;
+
}
+
 
+
.\-4u\28xlarge\29 {
+
margin-left: 33.33333%;
+
}
+
 
+
.\-3u\28xlarge\29 {
+
margin-left: 25%;
+
}
+
 
+
.\-2u\28xlarge\29 {
+
margin-left: 16.66667%;
+
}
+
 
+
.\-1u\28xlarge\29 {
+
margin-left: 8.33333%;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 1280px) {
+
 
+
.row > * {
+
padding: 0 0 0 1.5rem;
+
}
+
 
+
.row {
+
margin: 0 0 -1px -1.5rem;
+
}
+
 
+
.row.uniform > * {
+
padding: 1.5rem 0 0 1.5rem;
+
}
+
 
+
.row.uniform {
+
margin: -1.5rem 0 -1px -1.5rem;
+
}
+
 
+
.row.\32 00\25 > * {
+
padding: 0 0 0 3rem;
+
}
+
 
+
.row.\32 00\25 {
+
margin: 0 0 -1px -3rem;
+
}
+
 
+
.row.uniform.\32 00\25 > * {
+
padding: 3rem 0 0 3rem;
+
}
+
 
+
.row.uniform.\32 00\25 {
+
margin: -3rem 0 -1px -3rem;
+
}
+
 
+
.row.\31 50\25 > * {
+
padding: 0 0 0 2.25rem;
+
}
+
 
+
.row.\31 50\25 {
+
margin: 0 0 -1px -2.25rem;
+
}
+
 
+
.row.uniform.\31 50\25 > * {
+
padding: 2.25rem 0 0 2.25rem;
+
}
+
 
+
.row.uniform.\31 50\25 {
+
margin: -2.25rem 0 -1px -2.25rem;
+
}
+
 
+
.row.\35 0\25 > * {
+
padding: 0 0 0 0.75rem;
+
}
+
 
+
.row.\35 0\25 {
+
margin: 0 0 -1px -0.75rem;
+
}
+
 
+
.row.uniform.\35 0\25 > * {
+
padding: 0.75rem 0 0 0.75rem;
+
}
+
 
+
.row.uniform.\35 0\25 {
+
margin: -0.75rem 0 -1px -0.75rem;
+
}
+
 
+
.row.\32 5\25 > * {
+
padding: 0 0 0 0.375rem;
+
}
+
 
+
.row.\32 5\25 {
+
margin: 0 0 -1px -0.375rem;
+
}
+
 
+
.row.uniform.\32 5\25 > * {
+
padding: 0.375rem 0 0 0.375rem;
+
}
+
 
+
.row.uniform.\32 5\25 {
+
margin: -0.375rem 0 -1px -0.375rem;
+
}
+
 
+
.\31 2u\28large\29, .\31 2u\24\28large\29 {
+
width: 100%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 1u\28large\29, .\31 1u\24\28large\29 {
+
width: 91.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 0u\28large\29, .\31 0u\24\28large\29 {
+
width: 83.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\39 u\28large\29, .\39 u\24\28large\29 {
+
width: 75%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\38 u\28large\29, .\38 u\24\28large\29 {
+
width: 66.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\37 u\28large\29, .\37 u\24\28large\29 {
+
width: 58.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\36 u\28large\29, .\36 u\24\28large\29 {
+
width: 50%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\35 u\28large\29, .\35 u\24\28large\29 {
+
width: 41.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\34 u\28large\29, .\34 u\24\28large\29 {
+
width: 33.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\33 u\28large\29, .\33 u\24\28large\29 {
+
width: 25%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\32 u\28large\29, .\32 u\24\28large\29 {
+
width: 16.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 u\28large\29, .\31 u\24\28large\29 {
+
width: 8.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 2u\24\28large\29 + *,
+
.\31 1u\24\28large\29 + *,
+
.\31 0u\24\28large\29 + *,
+
.\39 u\24\28large\29 + *,
+
.\38 u\24\28large\29 + *,
+
.\37 u\24\28large\29 + *,
+
.\36 u\24\28large\29 + *,
+
.\35 u\24\28large\29 + *,
+
.\34 u\24\28large\29 + *,
+
.\33 u\24\28large\29 + *,
+
.\32 u\24\28large\29 + *,
+
.\31 u\24\28large\29 + * {
+
clear: left;
+
}
+
 
+
.\-11u\28large\29 {
+
margin-left: 91.66667%;
+
}
+
 
+
.\-10u\28large\29 {
+
margin-left: 83.33333%;
+
}
+
 
+
.\-9u\28large\29 {
+
margin-left: 75%;
+
}
+
 
+
.\-8u\28large\29 {
+
margin-left: 66.66667%;
+
}
+
 
+
.\-7u\28large\29 {
+
margin-left: 58.33333%;
+
}
+
 
+
.\-6u\28large\29 {
+
margin-left: 50%;
+
}
+
 
+
.\-5u\28large\29 {
+
margin-left: 41.66667%;
+
}
+
 
+
.\-4u\28large\29 {
+
margin-left: 33.33333%;
+
}
+
 
+
.\-3u\28large\29 {
+
margin-left: 25%;
+
}
+
 
+
.\-2u\28large\29 {
+
margin-left: 16.66667%;
+
}
+
 
+
.\-1u\28large\29 {
+
margin-left: 8.33333%;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 980px) {
+
 
+
.row > * {
+
padding: 0 0 0 1.5rem;
+
}
+
 
+
.row {
+
margin: 0 0 -1px -1.5rem;
+
}
+
 
+
.row.uniform > * {
+
padding: 1.5rem 0 0 1.5rem;
+
}
+
 
+
.row.uniform {
+
margin: -1.5rem 0 -1px -1.5rem;
+
}
+
 
+
.row.\32 00\25 > * {
+
padding: 0 0 0 3rem;
+
}
+
 
+
.row.\32 00\25 {
+
margin: 0 0 -1px -3rem;
+
}
+
 
+
.row.uniform.\32 00\25 > * {
+
padding: 3rem 0 0 3rem;
+
}
+
 
+
.row.uniform.\32 00\25 {
+
margin: -3rem 0 -1px -3rem;
+
}
+
 
+
.row.\31 50\25 > * {
+
padding: 0 0 0 2.25rem;
+
}
+
 
+
.row.\31 50\25 {
+
margin: 0 0 -1px -2.25rem;
+
}
+
 
+
.row.uniform.\31 50\25 > * {
+
padding: 2.25rem 0 0 2.25rem;
+
}
+
 
+
.row.uniform.\31 50\25 {
+
margin: -2.25rem 0 -1px -2.25rem;
+
}
+
 
+
.row.\35 0\25 > * {
+
padding: 0 0 0 0.75rem;
+
}
+
 
+
.row.\35 0\25 {
+
margin: 0 0 -1px -0.75rem;
+
}
+
 
+
.row.uniform.\35 0\25 > * {
+
padding: 0.75rem 0 0 0.75rem;
+
}
+
 
+
.row.uniform.\35 0\25 {
+
margin: -0.75rem 0 -1px -0.75rem;
+
}
+
 
+
.row.\32 5\25 > * {
+
padding: 0 0 0 0.375rem;
+
}
+
 
+
.row.\32 5\25 {
+
margin: 0 0 -1px -0.375rem;
+
}
+
 
+
.row.uniform.\32 5\25 > * {
+
padding: 0.375rem 0 0 0.375rem;
+
}
+
 
+
.row.uniform.\32 5\25 {
+
margin: -0.375rem 0 -1px -0.375rem;
+
}
+
 
+
.\31 2u\28medium\29, .\31 2u\24\28medium\29 {
+
width: 100%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 1u\28medium\29, .\31 1u\24\28medium\29 {
+
width: 91.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 0u\28medium\29, .\31 0u\24\28medium\29 {
+
width: 83.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\39 u\28medium\29, .\39 u\24\28medium\29 {
+
width: 75%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\38 u\28medium\29, .\38 u\24\28medium\29 {
+
width: 66.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\37 u\28medium\29, .\37 u\24\28medium\29 {
+
width: 58.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\36 u\28medium\29, .\36 u\24\28medium\29 {
+
width: 50%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\35 u\28medium\29, .\35 u\24\28medium\29 {
+
width: 41.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\34 u\28medium\29, .\34 u\24\28medium\29 {
+
width: 33.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\33 u\28medium\29, .\33 u\24\28medium\29 {
+
width: 25%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\32 u\28medium\29, .\32 u\24\28medium\29 {
+
width: 16.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 u\28medium\29, .\31 u\24\28medium\29 {
+
width: 8.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 2u\24\28medium\29 + *,
+
.\31 1u\24\28medium\29 + *,
+
.\31 0u\24\28medium\29 + *,
+
.\39 u\24\28medium\29 + *,
+
.\38 u\24\28medium\29 + *,
+
.\37 u\24\28medium\29 + *,
+
.\36 u\24\28medium\29 + *,
+
.\35 u\24\28medium\29 + *,
+
.\34 u\24\28medium\29 + *,
+
.\33 u\24\28medium\29 + *,
+
.\32 u\24\28medium\29 + *,
+
.\31 u\24\28medium\29 + * {
+
clear: left;
+
}
+
 
+
.\-11u\28medium\29 {
+
margin-left: 91.66667%;
+
}
+
 
+
.\-10u\28medium\29 {
+
margin-left: 83.33333%;
+
}
+
 
+
.\-9u\28medium\29 {
+
margin-left: 75%;
+
}
+
 
+
.\-8u\28medium\29 {
+
margin-left: 66.66667%;
+
}
+
 
+
.\-7u\28medium\29 {
+
margin-left: 58.33333%;
+
}
+
 
+
.\-6u\28medium\29 {
+
margin-left: 50%;
+
}
+
 
+
.\-5u\28medium\29 {
+
margin-left: 41.66667%;
+
}
+
 
+
.\-4u\28medium\29 {
+
margin-left: 33.33333%;
+
}
+
 
+
.\-3u\28medium\29 {
+
margin-left: 25%;
+
}
+
 
+
.\-2u\28medium\29 {
+
margin-left: 16.66667%;
+
}
+
 
+
.\-1u\28medium\29 {
+
margin-left: 8.33333%;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 736px) {
+
 
+
.row > * {
+
padding: 0 0 0 1.25rem;
+
}
+
 
+
.row {
+
margin: 0 0 -1px -1.25rem;
+
}
+
 
+
.row.uniform > * {
+
padding: 1.25rem 0 0 1.25rem;
+
}
+
 
+
.row.uniform {
+
margin: -1.25rem 0 -1px -1.25rem;
+
}
+
 
+
.row.\32 00\25 > * {
+
padding: 0 0 0 2.5rem;
+
}
+
 
+
.row.\32 00\25 {
+
margin: 0 0 -1px -2.5rem;
+
}
+
 
+
.row.uniform.\32 00\25 > * {
+
padding: 2.5rem 0 0 2.5rem;
+
}
+
 
+
.row.uniform.\32 00\25 {
+
margin: -2.5rem 0 -1px -2.5rem;
+
}
+
 
+
.row.\31 50\25 > * {
+
padding: 0 0 0 1.875rem;
+
}
+
 
+
.row.\31 50\25 {
+
margin: 0 0 -1px -1.875rem;
+
}
+
 
+
.row.uniform.\31 50\25 > * {
+
padding: 1.875rem 0 0 1.875rem;
+
}
+
 
+
.row.uniform.\31 50\25 {
+
margin: -1.875rem 0 -1px -1.875rem;
+
}
+
 
+
.row.\35 0\25 > * {
+
padding: 0 0 0 0.625rem;
+
}
+
 
+
.row.\35 0\25 {
+
margin: 0 0 -1px -0.625rem;
+
}
+
 
+
.row.uniform.\35 0\25 > * {
+
padding: 0.625rem 0 0 0.625rem;
+
}
+
 
+
.row.uniform.\35 0\25 {
+
margin: -0.625rem 0 -1px -0.625rem;
+
}
+
 
+
.row.\32 5\25 > * {
+
padding: 0 0 0 0.3125rem;
+
}
+
 
+
.row.\32 5\25 {
+
margin: 0 0 -1px -0.3125rem;
+
}
+
 
+
.row.uniform.\32 5\25 > * {
+
padding: 0.3125rem 0 0 0.3125rem;
+
}
+
 
+
.row.uniform.\32 5\25 {
+
margin: -0.3125rem 0 -1px -0.3125rem;
+
}
+
 
+
.\31 2u\28small\29, .\31 2u\24\28small\29 {
+
width: 100%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 1u\28small\29, .\31 1u\24\28small\29 {
+
width: 91.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 0u\28small\29, .\31 0u\24\28small\29 {
+
width: 83.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\39 u\28small\29, .\39 u\24\28small\29 {
+
width: 75%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\38 u\28small\29, .\38 u\24\28small\29 {
+
width: 66.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\37 u\28small\29, .\37 u\24\28small\29 {
+
width: 58.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\36 u\28small\29, .\36 u\24\28small\29 {
+
width: 50%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\35 u\28small\29, .\35 u\24\28small\29 {
+
width: 41.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\34 u\28small\29, .\34 u\24\28small\29 {
+
width: 33.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\33 u\28small\29, .\33 u\24\28small\29 {
+
width: 25%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\32 u\28small\29, .\32 u\24\28small\29 {
+
width: 16.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 u\28small\29, .\31 u\24\28small\29 {
+
width: 8.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 2u\24\28small\29 + *,
+
.\31 1u\24\28small\29 + *,
+
.\31 0u\24\28small\29 + *,
+
.\39 u\24\28small\29 + *,
+
.\38 u\24\28small\29 + *,
+
.\37 u\24\28small\29 + *,
+
.\36 u\24\28small\29 + *,
+
.\35 u\24\28small\29 + *,
+
.\34 u\24\28small\29 + *,
+
.\33 u\24\28small\29 + *,
+
.\32 u\24\28small\29 + *,
+
.\31 u\24\28small\29 + * {
+
clear: left;
+
}
+
 
+
.\-11u\28small\29 {
+
margin-left: 91.66667%;
+
}
+
 
+
.\-10u\28small\29 {
+
margin-left: 83.33333%;
+
}
+
 
+
.\-9u\28small\29 {
+
margin-left: 75%;
+
}
+
 
+
.\-8u\28small\29 {
+
margin-left: 66.66667%;
+
}
+
 
+
.\-7u\28small\29 {
+
margin-left: 58.33333%;
+
}
+
 
+
.\-6u\28small\29 {
+
margin-left: 50%;
+
}
+
 
+
.\-5u\28small\29 {
+
margin-left: 41.66667%;
+
}
+
 
+
.\-4u\28small\29 {
+
margin-left: 33.33333%;
+
}
+
 
+
.\-3u\28small\29 {
+
margin-left: 25%;
+
}
+
 
+
.\-2u\28small\29 {
+
margin-left: 16.66667%;
+
}
+
 
+
.\-1u\28small\29 {
+
margin-left: 8.33333%;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 480px) {
+
 
+
.row > * {
+
padding: 0 0 0 1.25rem;
+
}
+
 
+
.row {
+
margin: 0 0 -1px -1.25rem;
+
}
+
 
+
.row.uniform > * {
+
padding: 1.25rem 0 0 1.25rem;
+
}
+
 
+
.row.uniform {
+
margin: -1.25rem 0 -1px -1.25rem;
+
}
+
 
+
.row.\32 00\25 > * {
+
padding: 0 0 0 2.5rem;
+
}
+
 
+
.row.\32 00\25 {
+
margin: 0 0 -1px -2.5rem;
+
}
+
 
+
.row.uniform.\32 00\25 > * {
+
padding: 2.5rem 0 0 2.5rem;
+
}
+
 
+
.row.uniform.\32 00\25 {
+
margin: -2.5rem 0 -1px -2.5rem;
+
}
+
 
+
.row.\31 50\25 > * {
+
padding: 0 0 0 1.875rem;
+
}
+
 
+
.row.\31 50\25 {
+
margin: 0 0 -1px -1.875rem;
+
}
+
 
+
.row.uniform.\31 50\25 > * {
+
padding: 1.875rem 0 0 1.875rem;
+
}
+
 
+
.row.uniform.\31 50\25 {
+
margin: -1.875rem 0 -1px -1.875rem;
+
}
+
 
+
.row.\35 0\25 > * {
+
padding: 0 0 0 0.625rem;
+
}
+
 
+
.row.\35 0\25 {
+
margin: 0 0 -1px -0.625rem;
+
}
+
 
+
.row.uniform.\35 0\25 > * {
+
padding: 0.625rem 0 0 0.625rem;
+
}
+
 
+
.row.uniform.\35 0\25 {
+
margin: -0.625rem 0 -1px -0.625rem;
+
}
+
 
+
.row.\32 5\25 > * {
+
padding: 0 0 0 0.3125rem;
+
}
+
 
+
.row.\32 5\25 {
+
margin: 0 0 -1px -0.3125rem;
+
}
+
 
+
.row.uniform.\32 5\25 > * {
+
padding: 0.3125rem 0 0 0.3125rem;
+
}
+
 
+
.row.uniform.\32 5\25 {
+
margin: -0.3125rem 0 -1px -0.3125rem;
+
}
+
 
+
.\31 2u\28xsmall\29, .\31 2u\24\28xsmall\29 {
+
width: 100%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 1u\28xsmall\29, .\31 1u\24\28xsmall\29 {
+
width: 91.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 0u\28xsmall\29, .\31 0u\24\28xsmall\29 {
+
width: 83.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\39 u\28xsmall\29, .\39 u\24\28xsmall\29 {
+
width: 75%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\38 u\28xsmall\29, .\38 u\24\28xsmall\29 {
+
width: 66.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\37 u\28xsmall\29, .\37 u\24\28xsmall\29 {
+
width: 58.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\36 u\28xsmall\29, .\36 u\24\28xsmall\29 {
+
width: 50%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\35 u\28xsmall\29, .\35 u\24\28xsmall\29 {
+
width: 41.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\34 u\28xsmall\29, .\34 u\24\28xsmall\29 {
+
width: 33.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\33 u\28xsmall\29, .\33 u\24\28xsmall\29 {
+
width: 25%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\32 u\28xsmall\29, .\32 u\24\28xsmall\29 {
+
width: 16.6666666667%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 u\28xsmall\29, .\31 u\24\28xsmall\29 {
+
width: 8.3333333333%;
+
clear: none;
+
margin-left: 0;
+
}
+
 
+
.\31 2u\24\28xsmall\29 + *,
+
.\31 1u\24\28xsmall\29 + *,
+
.\31 0u\24\28xsmall\29 + *,
+
.\39 u\24\28xsmall\29 + *,
+
.\38 u\24\28xsmall\29 + *,
+
.\37 u\24\28xsmall\29 + *,
+
.\36 u\24\28xsmall\29 + *,
+
.\35 u\24\28xsmall\29 + *,
+
.\34 u\24\28xsmall\29 + *,
+
.\33 u\24\28xsmall\29 + *,
+
.\32 u\24\28xsmall\29 + *,
+
.\31 u\24\28xsmall\29 + * {
+
clear: left;
+
}
+
 
+
.\-11u\28xsmall\29 {
+
margin-left: 91.66667%;
+
}
+
 
+
.\-10u\28xsmall\29 {
+
margin-left: 83.33333%;
+
}
+
 
+
.\-9u\28xsmall\29 {
+
margin-left: 75%;
+
}
+
 
+
.\-8u\28xsmall\29 {
+
margin-left: 66.66667%;
+
}
+
 
+
.\-7u\28xsmall\29 {
+
margin-left: 58.33333%;
+
}
+
 
+
.\-6u\28xsmall\29 {
+
margin-left: 50%;
+
}
+
 
+
.\-5u\28xsmall\29 {
+
margin-left: 41.66667%;
+
}
+
 
+
.\-4u\28xsmall\29 {
+
margin-left: 33.33333%;
+
}
+
 
+
.\-3u\28xsmall\29 {
+
margin-left: 25%;
+
}
+
 
+
.\-2u\28xsmall\29 {
+
margin-left: 16.66667%;
+
}
+
 
+
.\-1u\28xsmall\29 {
+
margin-left: 8.33333%;
+
}
+
 
+
}
+
 
+
/* Basic */
+
 
+
@-ms-viewport {
+
width: device-width;
+
}
+
 
+
body {
+
-ms-overflow-style: scrollbar;
+
}
+
 
+
@media screen and (max-width: 480px) {
+
 
+
html, body {
+
min-width: 320px;
+
}
+
 
+
}
+
 
+
body {
+
background: #fff;
+
}
+
 
+
body.is-loading *, body.is-loading *:before, body.is-loading *:after {
+
-moz-animation: none !important;
+
-webkit-animation: none !important;
+
-ms-animation: none !important;
+
animation: none !important;
+
-moz-transition: none !important;
+
-webkit-transition: none !important;
+
-ms-transition: none !important;
+
transition: none !important;
+
}
+
 
+
/* Type */
+
 
+
html {
+
font-size: 13pt;
+
}
+
 
+
@media screen and (max-width: 1680px) {
+
 
+
html {
+
font-size: 11pt;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 1280px) {
+
 
+
html {
+
font-size: 11pt;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 980px) {
+
 
+
html {
+
font-size: 12pt;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 736px) {
+
 
+
html {
+
font-size: 12pt;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 480px) {
+
 
+
html {
+
font-size: 12pt;
+
}
+
 
+
}
+
 
+
body {
+
background-color: #fff;
+
color: #444;
+
}
+
 
+
body, input, select, textarea {
+
font-family: "Poppins", sans-serif;
+
font-weight: 300;
+
font-size: 1rem;
+
line-height: 1.65;
+
}
+
 
+
a {
+
text-decoration: underline;
+
}
+
 
+
a:hover {
+
text-decoration: none;
+
}
+
 
+
strong, b {
+
font-weight: 600;
+
}
+
 
+
em, i {
+
font-style: italic;
+
}
+
 
+
p {
+
margin: 0 0 2rem 0;
+
}
+
 
+
p.special {
+
text-transform: uppercase;
+
font-size: .75rem;
+
font-weight: 300;
+
margin: 0 0 .5rem 0;
+
padding: 0 0 1rem 0;
+
letter-spacing: .25rem;
+
}
+
 
+
p.special:after {
+
content: '';
+
position: absolute;
+
margin: auto;
+
right: 0;
+
bottom: 0;
+
left: 0;
+
width: 50%;
+
height: 1px;
+
background-color: rgba(0, 0, 0, 0.125);
+
}
+
 
+
h1, h2, h3, h4, h5, h6 {
+
font-weight: 300;
+
line-height: 1.5;
+
margin: 0 0 1rem 0;
+
}
+
 
+
h1 a, h2 a, h3 a, h4 a, h5 a, h6 a {
+
color: inherit;
+
text-decoration: none;
+
}
+
 
+
h2 {
+
font-size: 1.75rem;
+
}
+
 
+
h3 {
+
font-size: 1.35rem;
+
}
+
 
+
h4 {
+
font-size: 1.1rem;
+
}
+
 
+
h5 {
+
font-size: 0.9rem;
+
}
+
 
+
h6 {
+
font-size: 0.7rem;
+
}
+
 
+
sub {
+
font-size: 0.8rem;
+
position: relative;
+
top: 0.5rem;
+
}
+
 
+
sup {
+
font-size: 0.8rem;
+
position: relative;
+
top: -0.5rem;
+
}
+
 
+
blockquote {
+
border-left: solid 4px;
+
font-style: italic;
+
margin: 0 0 2rem 0;
+
padding: 0.5rem 0 0.5rem 2rem;
+
}
+
 
+
code {
+
border-radius: 2px;
+
border: solid 1px;
+
font-family: "Courier New", monospace;
+
font-size: 0.9rem;
+
margin: 0 0.25rem;
+
padding: 0.25rem 0.65rem;
+
}
+
 
+
pre {
+
-webkit-overflow-scrolling: touch;
+
font-family: "Courier New", monospace;
+
font-size: 0.9rem;
+
margin: 0 0 2rem 0;
+
}
+
 
+
pre code {
+
display: block;
+
line-height: 1.75;
+
padding: 1rem 1.5rem;
+
overflow-x: auto;
+
}
+
 
+
hr {
+
border: 0;
+
border-bottom: solid 1px;
+
margin: 2rem 0;
+
}
+
 
+
hr.major {
+
margin: 3rem 0;
+
}
+
 
+
.align-left {
+
text-align: left;
+
}
+
 
+
.align-center {
+
text-align: center;
+
}
+
 
+
.align-right {
+
text-align: right;
+
}
+
 
+
input, select, textarea {
+
color: #555;
+
}
+
 
+
a {
+
color: #8a4680;
+
}
+
 
+
strong, b {
+
color: #555;
+
}
+
 
+
h1, h2, h3, h4, h5, h6 {
+
color: #555;
+
}
+
 
+
blockquote {
+
border-left-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
code {
+
background: rgba(144, 144, 144, 0.075);
+
border-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
hr {
+
border-bottom-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
/* Box */
+
 
+
.box {
+
margin-bottom: 2rem;
+
background: #FFF;
+
}
+
 
+
.box .image.fit {
+
margin: 0;
+
border-radius: 0;
+
}
+
 
+
.box .image.fit img {
+
border-radius: 0;
+
}
+
 
+
.box header h2 {
+
margin-bottom: 2rem;
+
}
+
 
+
.box header p {
+
text-transform: uppercase;
+
font-size: .75rem;
+
font-weight: 300;
+
margin: 0 0 .25rem 0;
+
padding: 0 0 .75rem 0;
+
letter-spacing: .25rem;
+
}
+
 
+
.box header p:after {
+
content: '';
+
position: absolute;
+
margin: auto;
+
right: 0;
+
bottom: 0;
+
left: 0;
+
width: 50%;
+
height: 1px;
+
background-color: rgba(0, 0, 0, 0.125);
+
}
+
 
+
.box .content {
+
padding: 3rem;
+
}
+
 
+
.box > :last-child,
+
.box > :last-child > :last-child,
+
.box > :last-child > :last-child > :last-child {
+
margin-bottom: 0;
+
}
+
 
+
.box.alt {
+
border: 0;
+
border-radius: 0;
+
padding: 0;
+
}
+
 
+
@media screen and (max-width: 736px) {
+
 
+
.box .content {
+
padding: 2rem;
+
}
+
 
+
}
+
 
+
.box {
+
border-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
/* Button */
+
 
+
input[type="submit"],
+
input[type="reset"],
+
input[type="button"],
+
button,
+
.button {
+
-moz-appearance: none;
+
-webkit-appearance: none;
+
-ms-appearance: none;
+
appearance: none;
+
-moz-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
+
-webkit-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
+
-ms-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
+
transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
+
border-radius: 2px;
+
border: 0;
+
cursor: pointer;
+
display: inline-block;
+
font-weight: 300;
+
height: 2.85rem;
+
line-height: 2.95rem;
+
padding: 0 1.5rem;
+
text-align: center;
+
text-decoration: none;
+
text-transform: uppercase;
+
white-space: nowrap;
+
}
+
 
+
input[type="submit"].icon,
+
input[type="reset"].icon,
+
input[type="button"].icon,
+
button.icon,
+
.button.icon {
+
padding-left: 1.35rem;
+
}
+
 
+
input[type="submit"].icon:before,
+
input[type="reset"].icon:before,
+
input[type="button"].icon:before,
+
button.icon:before,
+
.button.icon:before {
+
margin-right: 0.5rem;
+
}
+
 
+
input[type="submit"].fit,
+
input[type="reset"].fit,
+
input[type="button"].fit,
+
button.fit,
+
.button.fit {
+
display: block;
+
margin: 0 0 1rem 0;
+
width: 100%;
+
}
+
 
+
input[type="submit"].small,
+
input[type="reset"].small,
+
input[type="button"].small,
+
button.small,
+
.button.small {
+
font-size: 0.8rem;
+
}
+
 
+
input[type="submit"].big,
+
input[type="reset"].big,
+
input[type="button"].big,
+
button.big,
+
.button.big {
+
font-size: 1.35rem;
+
}
+
 
+
input[type="submit"].disabled, input[type="submit"]:disabled,
+
input[type="reset"].disabled,
+
input[type="reset"]:disabled,
+
input[type="button"].disabled,
+
input[type="button"]:disabled,
+
button.disabled,
+
button:disabled,
+
.button.disabled,
+
.button:disabled {
+
-moz-pointer-events: none;
+
-webkit-pointer-events: none;
+
-ms-pointer-events: none;
+
pointer-events: none;
+
opacity: 0.25;
+
}
+
 
+
@media screen and (max-width: 480px) {
+
 
+
input[type="submit"],
+
input[type="reset"],
+
input[type="button"],
+
button,
+
.button {
+
padding: 0;
+
width: 100%;
+
}
+
 
+
}
+
 
+
input[type="submit"],
+
input[type="reset"],
+
input[type="button"],
+
button,
+
.button {
+
background-color: #f2f2f2;
+
color: #000 !important;
+
}
+
 
+
input[type="submit"]:hover,
+
input[type="reset"]:hover,
+
input[type="button"]:hover,
+
button:hover,
+
.button:hover {
+
background-color: white;
+
}
+
 
+
input[type="submit"]:active,
+
input[type="reset"]:active,
+
input[type="button"]:active,
+
button:active,
+
.button:active {
+
background-color: #e5e5e5;
+
}
+
 
+
input[type="submit"].alt,
+
input[type="reset"].alt,
+
input[type="button"].alt,
+
button.alt,
+
.button.alt {
+
background-color: transparent;
+
box-shadow: inset 0 0 0 2px rgba(144, 144, 144, 0.25);
+
color: #555 !important;
+
}
+
 
+
input[type="submit"].alt:hover,
+
input[type="reset"].alt:hover,
+
input[type="button"].alt:hover,
+
button.alt:hover,
+
.button.alt:hover {
+
background-color: rgba(144, 144, 144, 0.075);
+
}
+
 
+
input[type="submit"].alt:active,
+
input[type="reset"].alt:active,
+
input[type="button"].alt:active,
+
button.alt:active,
+
.button.alt:active {
+
background-color: rgba(144, 144, 144, 0.2);
+
}
+
 
+
input[type="submit"].alt.icon:before,
+
input[type="reset"].alt.icon:before,
+
input[type="button"].alt.icon:before,
+
button.alt.icon:before,
+
.button.alt.icon:before {
+
color: #bbb;
+
}
+
 
+
input[type="submit"].special,
+
input[type="reset"].special,
+
input[type="button"].special,
+
button.special,
+
.button.special {
+
background-color: #8a4680;
+
color: #ffffff !important;
+
}
+
 
+
input[type="submit"].special:hover,
+
input[type="reset"].special:hover,
+
input[type="button"].special:hover,
+
button.special:hover,
+
.button.special:hover {
+
background-color: #9b4f90;
+
}
+
 
+
input[type="submit"].special:active,
+
input[type="reset"].special:active,
+
input[type="button"].special:active,
+
button.special:active,
+
.button.special:active {
+
background-color: #793d70;
+
}
+
 
+
/* Form */
+
 
+
form {
+
margin: 0 0 2rem 0;
+
}
+
 
+
label {
+
display: block;
+
font-size: 0.9rem;
+
font-weight: 700;
+
margin: 0 0 1rem 0;
+
}
+
 
+
input[type="text"],
+
input[type="password"],
+
input[type="email"],
+
select,
+
textarea {
+
-moz-appearance: none;
+
-webkit-appearance: none;
+
-ms-appearance: none;
+
appearance: none;
+
border-radius: 2px;
+
border: none;
+
border: solid 1px;
+
color: inherit;
+
display: block;
+
outline: 0;
+
padding: 0 1rem;
+
text-decoration: none;
+
width: 100%;
+
}
+
 
+
input[type="text"]:invalid,
+
input[type="password"]:invalid,
+
input[type="email"]:invalid,
+
select:invalid,
+
textarea:invalid {
+
box-shadow: none;
+
}
+
 
+
.select-wrapper {
+
text-decoration: none;
+
display: block;
+
position: relative;
+
}
+
 
+
.select-wrapper:before {
+
-moz-osx-font-smoothing: grayscale;
+
-webkit-font-smoothing: antialiased;
+
font-family: FontAwesome;
+
font-style: normal;
+
font-weight: normal;
+
text-transform: none !important;
+
}
+
 
+
.select-wrapper:before {
+
content: '\f078';
+
display: block;
+
height: 2.75rem;
+
line-height: 2.75rem;
+
pointer-events: none;
+
position: absolute;
+
right: 0;
+
text-align: center;
+
top: 0;
+
width: 2.75rem;
+
}
+
 
+
.select-wrapper select::-ms-expand {
+
display: none;
+
}
+
 
+
input[type="text"],
+
input[type="password"],
+
input[type="email"],
+
select {
+
height: 2.75rem;
+
}
+
 
+
textarea {
+
padding: 0.75rem 1rem;
+
}
+
 
+
input[type="checkbox"],
+
input[type="radio"] {
+
-moz-appearance: none;
+
-webkit-appearance: none;
+
-ms-appearance: none;
+
appearance: none;
+
display: block;
+
float: left;
+
margin-right: -2rem;
+
opacity: 0;
+
width: 1rem;
+
z-index: -1;
+
}
+
 
+
input[type="checkbox"] + label,
+
input[type="radio"] + label {
+
text-decoration: none;
+
cursor: pointer;
+
display: inline-block;
+
font-size: 1rem;
+
font-weight: 400;
+
padding-left: 2.4rem;
+
padding-right: 0.75rem;
+
position: relative;
+
}
+
 
+
input[type="checkbox"] + label:before,
+
input[type="radio"] + label:before {
+
-moz-osx-font-smoothing: grayscale;
+
-webkit-font-smoothing: antialiased;
+
font-family: FontAwesome;
+
font-style: normal;
+
font-weight: normal;
+
text-transform: none !important;
+
}
+
 
+
input[type="checkbox"] + label:before,
+
input[type="radio"] + label:before {
+
border-radius: 2px;
+
border: solid 1px;
+
content: '';
+
display: inline-block;
+
height: 1.65rem;
+
left: 0;
+
line-height: 1.58125rem;
+
position: absolute;
+
text-align: center;
+
top: 0;
+
width: 1.65rem;
+
}
+
 
+
input[type="checkbox"]:checked + label:before,
+
input[type="radio"]:checked + label:before {
+
content: '\f00c';
+
}
+
 
+
input[type="checkbox"] + label:before {
+
border-radius: 2px;
+
}
+
 
+
input[type="radio"] + label:before {
+
border-radius: 100%;
+
}
+
 
+
::-webkit-input-placeholder {
+
opacity: 1.0;
+
}
+
 
+
:-moz-placeholder {
+
opacity: 1.0;
+
}
+
 
+
::-moz-placeholder {
+
opacity: 1.0;
+
}
+
 
+
:-ms-input-placeholder {
+
opacity: 1.0;
+
}
+
 
+
.formerize-placeholder {
+
opacity: 1.0;
+
}
+
 
+
label {
+
color: #555;
+
}
+
 
+
input[type="text"],
+
input[type="password"],
+
input[type="email"],
+
select,
+
textarea {
+
background: rgba(144, 144, 144, 0.075);
+
border-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
input[type="text"]:focus,
+
input[type="password"]:focus,
+
input[type="email"]:focus,
+
select:focus,
+
textarea:focus {
+
border-color: #8a4680;
+
box-shadow: 0 0 0 1px #8a4680;
+
}
+
 
+
.select-wrapper:before {
+
color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
input[type="checkbox"] + label,
+
input[type="radio"] + label {
+
color: #444;
+
}
+
 
+
input[type="checkbox"] + label:before,
+
input[type="radio"] + label:before {
+
background: rgba(144, 144, 144, 0.075);
+
border-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
input[type="checkbox"]:checked + label:before,
+
input[type="radio"]:checked + label:before {
+
background-color: #8a4680;
+
border-color: #8a4680;
+
color: #ffffff;
+
}
+
 
+
input[type="checkbox"]:focus + label:before,
+
input[type="radio"]:focus + label:before {
+
border-color: #8a4680;
+
box-shadow: 0 0 0 1px #8a4680;
+
}
+
 
+
::-webkit-input-placeholder {
+
color: #bbb !important;
+
}
+
 
+
:-moz-placeholder {
+
color: #bbb !important;
+
}
+
 
+
::-moz-placeholder {
+
color: #bbb !important;
+
}
+
 
+
:-ms-input-placeholder {
+
color: #bbb !important;
+
}
+
 
+
.formerize-placeholder {
+
color: #bbb !important;
+
}
+
 
+
/* Icon */
+
 
+
.icon {
+
text-decoration: none;
+
border-bottom: none;
+
position: relative;
+
}
+
 
+
.icon:before {
+
-moz-osx-font-smoothing: grayscale;
+
-webkit-font-smoothing: antialiased;
+
font-family: FontAwesome;
+
font-style: normal;
+
font-weight: normal;
+
text-transform: none !important;
+
}
+
 
+
.icon > .label {
+
display: none;
+
}
+
 
+
/* Image */
+
 
+
.image {
+
border-radius: 2px;
+
border: 0;
+
display: inline-block;
+
position: relative;
+
}
+
 
+
.image img {
+
border-radius: 2px;
+
display: block;
+
}
+
 
+
.image.left, .image.right {
+
max-width: 40%;
+
}
+
 
+
.image.left img, .image.right img {
+
width: 100%;
+
}
+
 
+
.image.left {
+
float: left;
+
margin: 0 1.5rem 1rem 0;
+
top: 0.25rem;
+
}
+
 
+
.image.right {
+
float: right;
+
margin: 0 0 1rem 1.5rem;
+
top: 0.25rem;
+
}
+
 
+
.image.fit {
+
display: block;
+
margin: 0 0 2rem 0;
+
width: 100%;
+
}
+
 
+
.image.fit img {
+
width: 100%;
+
}
+
 
+
.image.main {
+
display: block;
+
margin: 0 0 3rem 0;
+
width: 100%;
+
}
+
 
+
.image.main img {
+
width: 100%;
+
}
+
 
+
/* List */
+
 
+
ol {
+
list-style: decimal;
+
margin: 0 0 2rem 0;
+
padding-left: 1.25rem;
+
}
+
 
+
ol li {
+
padding-left: 0.25rem;
+
}
+
 
+
ul {
+
list-style: disc;
+
margin: 0 0 2rem 0;
+
padding-left: 1rem;
+
}
+
 
+
ul li {
+
padding-left: 0.5rem;
+
}
+
 
+
ul.alt {
+
list-style: none;
+
padding-left: 0;
+
}
+
 
+
ul.alt li {
+
border-top: solid 1px;
+
padding: 0.5rem 0;
+
}
+
 
+
ul.alt li:first-child {
+
border-top: 0;
+
padding-top: 0;
+
}
+
 
+
ul.icons {
+
cursor: default;
+
list-style: none;
+
padding-left: 0;
+
}
+
 
+
ul.icons li {
+
display: inline-block;
+
padding: 0 1rem 0 0;
+
}
+
 
+
ul.icons li:last-child {
+
padding-right: 0;
+
}
+
 
+
ul.icons li .icon:before {
+
font-size: 2rem;
+
}
+
 
+
ul.actions {
+
cursor: default;
+
list-style: none;
+
padding-left: 0;
+
}
+
 
+
ul.actions li {
+
display: inline-block;
+
padding: 0 1rem 0 0;
+
vertical-align: middle;
+
}
+
 
+
ul.actions li:last-child {
+
padding-right: 0;
+
}
+
 
+
ul.actions.small li {
+
padding: 0 0.5rem 0 0;
+
}
+
 
+
ul.actions.vertical li {
+
display: block;
+
padding: 1rem 0 0 0;
+
}
+
 
+
ul.actions.vertical li:first-child {
+
padding-top: 0;
+
}
+
 
+
ul.actions.vertical li > * {
+
margin-bottom: 0;
+
}
+
 
+
ul.actions.vertical.small li {
+
padding: 0.5rem 0 0 0;
+
}
+
 
+
ul.actions.vertical.small li:first-child {
+
padding-top: 0;
+
}
+
 
+
ul.actions.fit {
+
display: table;
+
margin-left: -1rem;
+
padding: 0;
+
table-layout: fixed;
+
width: calc(100% + 1rem);
+
}
+
 
+
ul.actions.fit li {
+
display: table-cell;
+
padding: 0 0 0 1rem;
+
}
+
 
+
ul.actions.fit li > * {
+
margin-bottom: 0;
+
}
+
 
+
ul.actions.fit.small {
+
margin-left: -0.5rem;
+
width: calc(100% + 0.5rem);
+
}
+
 
+
ul.actions.fit.small li {
+
padding: 0 0 0 0.5rem;
+
}
+
 
+
@media screen and (max-width: 480px) {
+
 
+
ul.actions {
+
margin: 0 0 2rem 0;
+
}
+
 
+
ul.actions li {
+
padding: 1rem 0 0 0;
+
display: block;
+
text-align: center;
+
width: 100%;
+
}
+
 
+
ul.actions li:first-child {
+
padding-top: 0;
+
}
+
 
+
ul.actions li > * {
+
width: 100%;
+
margin: 0 !important;
+
}
+
 
+
ul.actions li > *.icon:before {
+
margin-left: -2rem;
+
}
+
 
+
ul.actions.small li {
+
padding: 0.5rem 0 0 0;
+
}
+
 
+
ul.actions.small li:first-child {
+
padding-top: 0;
+
}
+
 
+
}
+
 
+
dl {
+
margin: 0 0 2rem 0;
+
}
+
 
+
dl dt {
+
display: block;
+
font-weight: 700;
+
margin: 0 0 1rem 0;
+
}
+
 
+
dl dd {
+
margin-left: 2rem;
+
}
+
 
+
ul.alt li {
+
border-top-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
/* Section/Article */
+
 
+
section.special, article.special {
+
text-align: center;
+
}
+
 
+
header p {
+
position: relative;
+
margin: 0 0 1.5rem 0;
+
}
+
 
+
header h2 + p {
+
font-size: 1.25rem;
+
margin-top: -1rem;
+
}
+
 
+
header h3 + p {
+
font-size: 1.1rem;
+
margin-top: -0.8rem;
+
}
+
 
+
header h4 + p,
+
header h5 + p,
+
header h6 + p {
+
font-size: 0.9rem;
+
margin-top: -0.6rem;
+
}
+
 
+
header p {
+
color: #bbb;
+
}
+
 
+
/* Table */
+
 
+
.table-wrapper {
+
-webkit-overflow-scrolling: touch;
+
overflow-x: auto;
+
}
+
 
+
table {
+
margin: 0 0 2rem 0;
+
width: 100%;
+
}
+
 
+
table tbody tr {
+
border: solid 1px;
+
border-left: 0;
+
border-right: 0;
+
}
+
 
+
table td {
+
padding: 0.75rem 0.75rem;
+
}
+
 
+
table th {
+
font-size: 0.9rem;
+
font-weight: 700;
+
padding: 0 0.75rem 0.75rem 0.75rem;
+
text-align: left;
+
}
+
 
+
table thead {
+
border-bottom: solid 2px;
+
}
+
 
+
table tfoot {
+
border-top: solid 2px;
+
}
+
 
+
table.alt {
+
border-collapse: separate;
+
}
+
 
+
table.alt tbody tr td {
+
border: solid 1px;
+
border-left-width: 0;
+
border-top-width: 0;
+
}
+
 
+
table.alt tbody tr td:first-child {
+
border-left-width: 1px;
+
}
+
 
+
table.alt tbody tr:first-child td {
+
border-top-width: 1px;
+
}
+
 
+
table.alt thead {
+
border-bottom: 0;
+
}
+
 
+
table.alt tfoot {
+
border-top: 0;
+
}
+
 
+
table tbody tr {
+
border-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
table tbody tr:nth-child(2n + 1) {
+
background-color: rgba(144, 144, 144, 0.075);
+
}
+
 
+
table th {
+
color: #555;
+
}
+
 
+
table thead {
+
border-bottom-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
table tfoot {
+
border-top-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
table.alt tbody tr td {
+
border-color: rgba(144, 144, 144, 0.25);
+
}
+
 
+
/* Wrapper */
+
 
+
.wrapper {
+
padding: 6rem 0 4rem 0 ;
+
position: relative;
+
margin: 0;
+
}
+
 
+
.wrapper > .inner {
+
margin: 0 auto;
+
width: 80rem;
+
}
+
 
+
.wrapper.style1 {
+
background-color: #8a4680;
+
color: #e2d1df;
+
}
+
 
+
.wrapper.style1 input, .wrapper.style1 select, .wrapper.style1 textarea {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style1 a {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style1 strong, .wrapper.style1 b {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style1 h1, .wrapper.style1 h2, .wrapper.style1 h3, .wrapper.style1 h4, .wrapper.style1 h5, .wrapper.style1 h6 {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style1 blockquote {
+
border-left-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 code {
+
background: rgba(255, 255, 255, 0.075);
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 hr {
+
border-bottom-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 .box {
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 input[type="submit"],
+
.wrapper.style1 input[type="reset"],
+
.wrapper.style1 input[type="button"],
+
.wrapper.style1 button,
+
.wrapper.style1 .button {
+
background-color: #f2f2f2;
+
color: #000 !important;
+
}
+
 
+
.wrapper.style1 input[type="submit"]:hover,
+
.wrapper.style1 input[type="reset"]:hover,
+
.wrapper.style1 input[type="button"]:hover,
+
.wrapper.style1 button:hover,
+
.wrapper.style1 .button:hover {
+
background-color: white;
+
}
+
 
+
.wrapper.style1 input[type="submit"]:active,
+
.wrapper.style1 input[type="reset"]:active,
+
.wrapper.style1 input[type="button"]:active,
+
.wrapper.style1 button:active,
+
.wrapper.style1 .button:active {
+
background-color: #e5e5e5;
+
}
+
 
+
.wrapper.style1 input[type="submit"].alt,
+
.wrapper.style1 input[type="reset"].alt,
+
.wrapper.style1 input[type="button"].alt,
+
.wrapper.style1 button.alt,
+
.wrapper.style1 .button.alt {
+
background-color: transparent;
+
box-shadow: inset 0 0 0 2px rgba(255, 255, 255, 0.25);
+
color: #ffffff !important;
+
}
+
 
+
.wrapper.style1 input[type="submit"].alt:hover,
+
.wrapper.style1 input[type="reset"].alt:hover,
+
.wrapper.style1 input[type="button"].alt:hover,
+
.wrapper.style1 button.alt:hover,
+
.wrapper.style1 .button.alt:hover {
+
background-color: rgba(255, 255, 255, 0.075);
+
}
+
 
+
.wrapper.style1 input[type="submit"].alt:active,
+
.wrapper.style1 input[type="reset"].alt:active,
+
.wrapper.style1 input[type="button"].alt:active,
+
.wrapper.style1 button.alt:active,
+
.wrapper.style1 .button.alt:active {
+
background-color: rgba(255, 255, 255, 0.2);
+
}
+
 
+
.wrapper.style1 input[type="submit"].alt.icon:before,
+
.wrapper.style1 input[type="reset"].alt.icon:before,
+
.wrapper.style1 input[type="button"].alt.icon:before,
+
.wrapper.style1 button.alt.icon:before,
+
.wrapper.style1 .button.alt.icon:before {
+
color: #d0b5cc;
+
}
+
 
+
.wrapper.style1 input[type="submit"].special,
+
.wrapper.style1 input[type="reset"].special,
+
.wrapper.style1 input[type="button"].special,
+
.wrapper.style1 button.special,
+
.wrapper.style1 .button.special {
+
background-color: #ffffff;
+
color: #8a4680 !important;
+
}
+
 
+
.wrapper.style1 label {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style1 input[type="text"],
+
.wrapper.style1 input[type="password"],
+
.wrapper.style1 input[type="email"],
+
.wrapper.style1 select,
+
.wrapper.style1 textarea {
+
background: rgba(255, 255, 255, 0.075);
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 input[type="text"]:focus,
+
.wrapper.style1 input[type="password"]:focus,
+
.wrapper.style1 input[type="email"]:focus,
+
.wrapper.style1 select:focus,
+
.wrapper.style1 textarea:focus {
+
border-color: #ffffff;
+
box-shadow: 0 0 0 1px #ffffff;
+
}
+
 
+
.wrapper.style1 .select-wrapper:before {
+
color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 input[type="checkbox"] + label,
+
.wrapper.style1 input[type="radio"] + label {
+
color: #e2d1df;
+
}
+
 
+
.wrapper.style1 input[type="checkbox"] + label:before,
+
.wrapper.style1 input[type="radio"] + label:before {
+
background: rgba(255, 255, 255, 0.075);
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 input[type="checkbox"]:checked + label:before,
+
.wrapper.style1 input[type="radio"]:checked + label:before {
+
background-color: #ffffff;
+
border-color: #ffffff;
+
color: #8a4680;
+
}
+
 
+
.wrapper.style1 input[type="checkbox"]:focus + label:before,
+
.wrapper.style1 input[type="radio"]:focus + label:before {
+
border-color: #ffffff;
+
box-shadow: 0 0 0 1px #ffffff;
+
}
+
 
+
.wrapper.style1 ::-webkit-input-placeholder {
+
color: #d0b5cc !important;
+
}
+
 
+
.wrapper.style1 :-moz-placeholder {
+
color: #d0b5cc !important;
+
}
+
 
+
.wrapper.style1 ::-moz-placeholder {
+
color: #d0b5cc !important;
+
}
+
 
+
.wrapper.style1 :-ms-input-placeholder {
+
color: #d0b5cc !important;
+
}
+
 
+
.wrapper.style1 .formerize-placeholder {
+
color: #d0b5cc !important;
+
}
+
 
+
.wrapper.style1 ul.alt li {
+
border-top-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 header p {
+
color: #d0b5cc;
+
}
+
 
+
.wrapper.style1 table tbody tr {
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 table tbody tr:nth-child(2n + 1) {
+
background-color: rgba(255, 255, 255, 0.075);
+
}
+
 
+
.wrapper.style1 table th {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style1 table thead {
+
border-bottom-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 table tfoot {
+
border-top-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style1 table.alt tbody tr td {
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style2 {
+
background-color: #f2f2f2;
+
color: #a6a6a6;
+
}
+
 
+
.wrapper.style2 input, .wrapper.style2 select, .wrapper.style2 textarea {
+
color: #000;
+
}
+
 
+
.wrapper.style2 a {
+
color: #8a4680;
+
}
+
 
+
.wrapper.style2 strong, .wrapper.style2 b {
+
color: #000;
+
}
+
 
+
.wrapper.style2 h1, .wrapper.style2 h2, .wrapper.style2 h3, .wrapper.style2 h4, .wrapper.style2 h5, .wrapper.style2 h6 {
+
color: #000;
+
}
+
 
+
.wrapper.style2 blockquote {
+
border-left-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 code {
+
background: rgba(0, 0, 0, 0.075);
+
border-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 hr {
+
border-bottom-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 .box {
+
border-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 input[type="submit"],
+
.wrapper.style2 input[type="reset"],
+
.wrapper.style2 input[type="button"],
+
.wrapper.style2 button,
+
.wrapper.style2 .button {
+
background-color: #000;
+
color: #f2f2f2 !important;
+
}
+
 
+
.wrapper.style2 input[type="submit"].alt,
+
.wrapper.style2 input[type="reset"].alt,
+
.wrapper.style2 input[type="button"].alt,
+
.wrapper.style2 button.alt,
+
.wrapper.style2 .button.alt {
+
background-color: transparent;
+
box-shadow: inset 0 0 0 2px rgba(0, 0, 0, 0.15);
+
color: #000 !important;
+
}
+
 
+
.wrapper.style2 input[type="submit"].alt:hover,
+
.wrapper.style2 input[type="reset"].alt:hover,
+
.wrapper.style2 input[type="button"].alt:hover,
+
.wrapper.style2 button.alt:hover,
+
.wrapper.style2 .button.alt:hover {
+
background-color: rgba(0, 0, 0, 0.075);
+
}
+
 
+
.wrapper.style2 input[type="submit"].alt:active,
+
.wrapper.style2 input[type="reset"].alt:active,
+
.wrapper.style2 input[type="button"].alt:active,
+
.wrapper.style2 button.alt:active,
+
.wrapper.style2 .button.alt:active {
+
background-color: rgba(0, 0, 0, 0.2);
+
}
+
 
+
.wrapper.style2 input[type="submit"].alt.icon:before,
+
.wrapper.style2 input[type="reset"].alt.icon:before,
+
.wrapper.style2 input[type="button"].alt.icon:before,
+
.wrapper.style2 button.alt.icon:before,
+
.wrapper.style2 .button.alt.icon:before {
+
color: #999999;
+
}
+
 
+
.wrapper.style2 input[type="submit"].special,
+
.wrapper.style2 input[type="reset"].special,
+
.wrapper.style2 input[type="button"].special,
+
.wrapper.style2 button.special,
+
.wrapper.style2 .button.special {
+
background-color: #8a4680;
+
color: #ffffff !important;
+
}
+
 
+
.wrapper.style2 input[type="submit"].special:hover,
+
.wrapper.style2 input[type="reset"].special:hover,
+
.wrapper.style2 input[type="button"].special:hover,
+
.wrapper.style2 button.special:hover,
+
.wrapper.style2 .button.special:hover {
+
background-color: #9b4f90;
+
}
+
 
+
.wrapper.style2 input[type="submit"].special:active,
+
.wrapper.style2 input[type="reset"].special:active,
+
.wrapper.style2 input[type="button"].special:active,
+
.wrapper.style2 button.special:active,
+
.wrapper.style2 .button.special:active {
+
background-color: #793d70;
+
}
+
 
+
.wrapper.style2 label {
+
color: #000;
+
}
+
 
+
.wrapper.style2 input[type="text"],
+
.wrapper.style2 input[type="password"],
+
.wrapper.style2 input[type="email"],
+
.wrapper.style2 select,
+
.wrapper.style2 textarea {
+
background: rgba(0, 0, 0, 0.075);
+
border-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 input[type="text"]:focus,
+
.wrapper.style2 input[type="password"]:focus,
+
.wrapper.style2 input[type="email"]:focus,
+
.wrapper.style2 select:focus,
+
.wrapper.style2 textarea:focus {
+
border-color: #8a4680;
+
box-shadow: 0 0 0 1px #8a4680;
+
}
+
 
+
.wrapper.style2 .select-wrapper:before {
+
color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 input[type="checkbox"] + label,
+
.wrapper.style2 input[type="radio"] + label {
+
color: #a6a6a6;
+
}
+
 
+
.wrapper.style2 input[type="checkbox"] + label:before,
+
.wrapper.style2 input[type="radio"] + label:before {
+
background: rgba(0, 0, 0, 0.075);
+
border-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 input[type="checkbox"]:checked + label:before,
+
.wrapper.style2 input[type="radio"]:checked + label:before {
+
background-color: #8a4680;
+
border-color: #8a4680;
+
color: #ffffff;
+
}
+
 
+
.wrapper.style2 input[type="checkbox"]:focus + label:before,
+
.wrapper.style2 input[type="radio"]:focus + label:before {
+
border-color: #8a4680;
+
box-shadow: 0 0 0 1px #8a4680;
+
}
+
 
+
.wrapper.style2 ::-webkit-input-placeholder {
+
color: #999999 !important;
+
}
+
 
+
.wrapper.style2 :-moz-placeholder {
+
color: #999999 !important;
+
}
+
 
+
.wrapper.style2 ::-moz-placeholder {
+
color: #999999 !important;
+
}
+
 
+
.wrapper.style2 :-ms-input-placeholder {
+
color: #999999 !important;
+
}
+
 
+
.wrapper.style2 .formerize-placeholder {
+
color: #999999 !important;
+
}
+
 
+
.wrapper.style2 ul.alt li {
+
border-top-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 header p {
+
color: #999999;
+
}
+
 
+
.wrapper.style2 table tbody tr {
+
border-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 table tbody tr:nth-child(2n + 1) {
+
background-color: rgba(0, 0, 0, 0.075);
+
}
+
 
+
.wrapper.style2 table th {
+
color: #000;
+
}
+
 
+
.wrapper.style2 table thead {
+
border-bottom-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 table tfoot {
+
border-top-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style2 table.alt tbody tr td {
+
border-color: rgba(0, 0, 0, 0.15);
+
}
+
 
+
.wrapper.style3 {
+
background-color: #000;
+
color: #bfbfbf;
+
background-image: url(../../images/bg.jpg);
+
background-size: cover;
+
background-attachment: fixed;
+
background-position: center;
+
position: relative;
+
}
+
 
+
.wrapper.style3 input, .wrapper.style3 select, .wrapper.style3 textarea {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style3 a {
+
color: #8a4680;
+
}
+
 
+
.wrapper.style3 strong, .wrapper.style3 b {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style3 h1, .wrapper.style3 h2, .wrapper.style3 h3, .wrapper.style3 h4, .wrapper.style3 h5, .wrapper.style3 h6 {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style3 blockquote {
+
border-left-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 code {
+
background: rgba(255, 255, 255, 0.075);
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 hr {
+
border-bottom-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 .box {
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 input[type="submit"],
+
.wrapper.style3 input[type="reset"],
+
.wrapper.style3 input[type="button"],
+
.wrapper.style3 button,
+
.wrapper.style3 .button {
+
background-color: #f2f2f2;
+
color: #000 !important;
+
}
+
 
+
.wrapper.style3 input[type="submit"]:hover,
+
.wrapper.style3 input[type="reset"]:hover,
+
.wrapper.style3 input[type="button"]:hover,
+
.wrapper.style3 button:hover,
+
.wrapper.style3 .button:hover {
+
background-color: white;
+
}
+
 
+
.wrapper.style3 input[type="submit"]:active,
+
.wrapper.style3 input[type="reset"]:active,
+
.wrapper.style3 input[type="button"]:active,
+
.wrapper.style3 button:active,
+
.wrapper.style3 .button:active {
+
background-color: #e5e5e5;
+
}
+
 
+
.wrapper.style3 input[type="submit"].alt,
+
.wrapper.style3 input[type="reset"].alt,
+
.wrapper.style3 input[type="button"].alt,
+
.wrapper.style3 button.alt,
+
.wrapper.style3 .button.alt {
+
background-color: transparent;
+
box-shadow: inset 0 0 0 2px rgba(255, 255, 255, 0.25);
+
color: #ffffff !important;
+
}
+
 
+
.wrapper.style3 input[type="submit"].alt:hover,
+
.wrapper.style3 input[type="reset"].alt:hover,
+
.wrapper.style3 input[type="button"].alt:hover,
+
.wrapper.style3 button.alt:hover,
+
.wrapper.style3 .button.alt:hover {
+
background-color: rgba(255, 255, 255, 0.075);
+
}
+
 
+
.wrapper.style3 input[type="submit"].alt:active,
+
.wrapper.style3 input[type="reset"].alt:active,
+
.wrapper.style3 input[type="button"].alt:active,
+
.wrapper.style3 button.alt:active,
+
.wrapper.style3 .button.alt:active {
+
background-color: rgba(255, 255, 255, 0.2);
+
}
+
 
+
.wrapper.style3 input[type="submit"].alt.icon:before,
+
.wrapper.style3 input[type="reset"].alt.icon:before,
+
.wrapper.style3 input[type="button"].alt.icon:before,
+
.wrapper.style3 button.alt.icon:before,
+
.wrapper.style3 .button.alt.icon:before {
+
color: #999999;
+
}
+
 
+
.wrapper.style3 input[type="submit"].special,
+
.wrapper.style3 input[type="reset"].special,
+
.wrapper.style3 input[type="button"].special,
+
.wrapper.style3 button.special,
+
.wrapper.style3 .button.special {
+
background-color: #8a4680;
+
color: #ffffff !important;
+
}
+
 
+
.wrapper.style3 input[type="submit"].special:hover,
+
.wrapper.style3 input[type="reset"].special:hover,
+
.wrapper.style3 input[type="button"].special:hover,
+
.wrapper.style3 button.special:hover,
+
.wrapper.style3 .button.special:hover {
+
background-color: #9b4f90;
+
}
+
 
+
.wrapper.style3 input[type="submit"].special:active,
+
.wrapper.style3 input[type="reset"].special:active,
+
.wrapper.style3 input[type="button"].special:active,
+
.wrapper.style3 button.special:active,
+
.wrapper.style3 .button.special:active {
+
background-color: #793d70;
+
}
+
 
+
.wrapper.style3 label {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style3 input[type="text"],
+
.wrapper.style3 input[type="password"],
+
.wrapper.style3 input[type="email"],
+
.wrapper.style3 select,
+
.wrapper.style3 textarea {
+
background: rgba(255, 255, 255, 0.075);
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 input[type="text"]:focus,
+
.wrapper.style3 input[type="password"]:focus,
+
.wrapper.style3 input[type="email"]:focus,
+
.wrapper.style3 select:focus,
+
.wrapper.style3 textarea:focus {
+
border-color: #8a4680;
+
box-shadow: 0 0 0 1px #8a4680;
+
}
+
 
+
.wrapper.style3 .select-wrapper:before {
+
color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 input[type="checkbox"] + label,
+
.wrapper.style3 input[type="radio"] + label {
+
color: #bfbfbf;
+
}
+
 
+
.wrapper.style3 input[type="checkbox"] + label:before,
+
.wrapper.style3 input[type="radio"] + label:before {
+
background: rgba(255, 255, 255, 0.075);
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 input[type="checkbox"]:checked + label:before,
+
.wrapper.style3 input[type="radio"]:checked + label:before {
+
background-color: #8a4680;
+
border-color: #8a4680;
+
color: #ffffff;
+
}
+
 
+
.wrapper.style3 input[type="checkbox"]:focus + label:before,
+
.wrapper.style3 input[type="radio"]:focus + label:before {
+
border-color: #8a4680;
+
box-shadow: 0 0 0 1px #8a4680;
+
}
+
 
+
.wrapper.style3 ::-webkit-input-placeholder {
+
color: #999999 !important;
+
}
+
 
+
.wrapper.style3 :-moz-placeholder {
+
color: #999999 !important;
+
}
+
 
+
.wrapper.style3 ::-moz-placeholder {
+
color: #999999 !important;
+
}
+
 
+
.wrapper.style3 :-ms-input-placeholder {
+
color: #999999 !important;
+
}
+
 
+
.wrapper.style3 .formerize-placeholder {
+
color: #999999 !important;
+
}
+
 
+
.wrapper.style3 ul.alt li {
+
border-top-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 header p {
+
color: #999999;
+
}
+
 
+
.wrapper.style3 table tbody tr {
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 table tbody tr:nth-child(2n + 1) {
+
background-color: rgba(255, 255, 255, 0.075);
+
}
+
 
+
.wrapper.style3 table th {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style3 table thead {
+
border-bottom-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 table tfoot {
+
border-top-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 table.alt tbody tr td {
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.wrapper.style3 .inner {
+
position: relative;
+
z-index: 2;
+
}
+
 
+
.wrapper.style3 p {
+
text-transform: uppercase;
+
font-size: .75rem;
+
font-weight: 300;
+
margin: 0 0 .5rem 0;
+
padding: 0 0 1rem 0;
+
letter-spacing: .25rem;
+
}
+
 
+
.wrapper.style3 p:after {
+
content: '';
+
position: absolute;
+
margin: auto;
+
right: 0;
+
bottom: 0;
+
left: 0;
+
width: 50%;
+
height: 1px;
+
background-color: rgba(255, 255, 255, 0.5);
+
}
+
 
+
.wrapper.style3:before {
+
-moz-transition: opacity 3s ease;
+
-webkit-transition: opacity 3s ease;
+
-ms-transition: opacity 3s ease;
+
transition: opacity 3s ease;
+
-moz-transition-delay: 1.25s;
+
-webkit-transition-delay: 1.25s;
+
-ms-transition-delay: 1.25s;
+
transition-delay: 1.25s;
+
content: '';
+
display: block;
+
background-color: rgba(0, 0, 0, 0.95);
+
height: 100%;
+
left: 0;
+
opacity: 0.75;
+
position: absolute;
+
top: 0;
+
width: 100%;
+
z-index: 1;
+
}
+
 
+
@media screen and (max-width: 1280px) {
+
 
+
.wrapper > .inner {
+
width: 75rem;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 980px) {
+
 
+
.wrapper > .inner {
+
width: 90%;
+
}
+
 
+
.wrapper.style3 {
+
background-attachment: scroll;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 736px) {
+
 
+
.wrapper {
+
padding: 3rem 0 1rem 0 ;
+
}
+
 
+
}
+
 
+
/* Flexgrid */
+
 
+
.grid-style {
+
width: 100%;
+
margin: 0 0 2.5rem 0;
+
display: -moz-flex;
+
display: -webkit-flex;
+
display: -ms-flex;
+
display: flex;
+
-moz-flex-wrap: wrap;
+
-webkit-flex-wrap: wrap;
+
-ms-flex-wrap: wrap;
+
flex-wrap: wrap;
+
-moz-align-items: stretch;
+
-webkit-align-items: stretch;
+
-ms-align-items: stretch;
+
align-items: stretch;
+
}
+
 
+
.grid-style > * {
+
-moz-flex-shrink: 1;
+
-webkit-flex-shrink: 1;
+
-ms-flex-shrink: 1;
+
flex-shrink: 1;
+
-moz-flex-grow: 0;
+
-webkit-flex-grow: 0;
+
-ms-flex-grow: 0;
+
flex-grow: 0;
+
}
+
 
+
.grid-style > * {
+
width: 50%;
+
}
+
 
+
.grid-style > * {
+
padding: 2rem;
+
width: calc(50% + 2rem);
+
}
+
 
+
.grid-style > :nth-child(-n + 2) {
+
padding-top: 0;
+
}
+
 
+
.grid-style > :nth-last-child(-n + 2) {
+
padding-bottom: 0;
+
}
+
 
+
.grid-style > :nth-child(2n + 1) {
+
padding-left: 0;
+
}
+
 
+
.grid-style > :nth-child(2n) {
+
padding-right: 0;
+
}
+
 
+
.grid-style > :nth-child(2n + 1),
+
.grid-style > :nth-child(2n) {
+
width: calc(50% + 0rem);
+
}
+
 
+
.grid-style .box {
+
margin: 0;
+
}
+
 
+
@media screen and (max-width: 980px) {
+
 
+
.grid-style > * {
+
width: 100%;
+
}
+
 
+
.grid-style > * {
+
padding: 1rem;
+
width: calc(50% + 1rem);
+
}
+
 
+
.grid-style > :nth-child(-n + 2) {
+
padding-top: 1rem;
+
}
+
 
+
.grid-style > :nth-last-child(-n + 2) {
+
padding-bottom: 1rem;
+
}
+
 
+
.grid-style > :nth-child(2n + 1) {
+
padding-left: 1rem;
+
}
+
 
+
.grid-style > :nth-child(2n) {
+
padding-right: 1rem;
+
}
+
 
+
.grid-style > :nth-child(2n + 1),
+
.grid-style > :nth-child(2n) {
+
padding: 1rem;
+
width: calc(100% + 2rem);
+
}
+
 
+
.grid-style > * {
+
padding: 1rem;
+
width: calc(100% + 2rem);
+
}
+
 
+
.grid-style > :nth-child(-n + 1) {
+
padding-top: 0;
+
}
+
 
+
.grid-style > :nth-last-child(-n + 1) {
+
padding-bottom: 0;
+
}
+
 
+
.grid-style > :nth-child(1n + 1) {
+
padding-left: 0;
+
}
+
 
+
.grid-style > :nth-child(1n) {
+
padding-right: 0;
+
}
+
 
+
.grid-style > :nth-child(1n + 1),
+
.grid-style > :nth-child(1n) {
+
width: calc(100% + 1rem);
+
}
+
 
+
}
+
 
+
.gallery {
+
width: 100%;
+
margin: 2.5rem 0 2.5rem 0;
+
display: -moz-flex;
+
display: -webkit-flex;
+
display: -ms-flex;
+
display: flex;
+
-moz-flex-wrap: wrap;
+
-webkit-flex-wrap: wrap;
+
-ms-flex-wrap: wrap;
+
flex-wrap: wrap;
+
-moz-align-items: stretch;
+
-webkit-align-items: stretch;
+
-ms-align-items: stretch;
+
align-items: stretch;
+
}
+
 
+
.gallery > * {
+
-moz-flex-shrink: 1;
+
-webkit-flex-shrink: 1;
+
-ms-flex-shrink: 1;
+
flex-shrink: 1;
+
-moz-flex-grow: 0;
+
-webkit-flex-grow: 0;
+
-ms-flex-grow: 0;
+
flex-grow: 0;
+
}
+
 
+
.gallery > * {
+
width: 50%;
+
}
+
 
+
.gallery > * {
+
padding: 2rem;
+
width: calc(50% + 2rem);
+
}
+
 
+
.gallery > :nth-child(-n + 2) {
+
padding-top: 0;
+
}
+
 
+
.gallery > :nth-last-child(-n + 2) {
+
padding-bottom: 0;
+
}
+
 
+
.gallery > :nth-child(2n + 1) {
+
padding-left: 0;
+
}
+
 
+
.gallery > :nth-child(2n) {
+
padding-right: 0;
+
}
+
 
+
.gallery > :nth-child(2n + 1),
+
.gallery > :nth-child(2n) {
+
width: calc(50% + 0rem);
+
}
+
 
+
.gallery .image {
+
background: #FFF;
+
padding: 1rem;
+
}
+
 
+
.gallery .image.fit {
+
margin: 0;
+
}
+
 
+
@media screen and (max-width: 980px) {
+
 
+
.gallery > * {
+
width: 100%;
+
}
+
 
+
.gallery > * {
+
padding: 1rem;
+
width: calc(50% + 1rem);
+
}
+
 
+
.gallery > :nth-child(-n + 2) {
+
padding-top: 1rem;
+
}
+
 
+
.gallery > :nth-last-child(-n + 2) {
+
padding-bottom: 1rem;
+
}
+
 
+
.gallery > :nth-child(2n + 1) {
+
padding-left: 1rem;
+
}
+
 
+
.gallery > :nth-child(2n) {
+
padding-right: 1rem;
+
}
+
 
+
.gallery > :nth-child(2n + 1),
+
.gallery > :nth-child(2n) {
+
padding: 1rem;
+
width: calc(100% + 2rem);
+
}
+
 
+
.gallery > * {
+
padding: 1rem;
+
width: calc(100% + 2rem);
+
}
+
 
+
.gallery > :nth-child(-n + 1) {
+
padding-top: 0;
+
}
+
 
+
.gallery > :nth-last-child(-n + 1) {
+
padding-bottom: 0;
+
}
+
 
+
.gallery > :nth-child(1n + 1) {
+
padding-left: 0;
+
}
+
 
+
.gallery > :nth-child(1n) {
+
padding-right: 0;
+
}
+
 
+
.gallery > :nth-child(1n + 1),
+
.gallery > :nth-child(1n) {
+
width: calc(100% + 1rem);
+
}
+
 
+
}
+
 
+
/* Header */
+
 
+
body.subpage {
+
padding-top: 3.25em;
+
}
+
 
+
@-moz-keyframes reveal-header {
+
0% {
+
top: -4em;
+
opacity: 0;
+
}
+
 
+
100% {
+
top: 0;
+
opacity: 1;
+
}
+
}
+
 
+
@-webkit-keyframes reveal-header {
+
0% {
+
top: -4em;
+
opacity: 0;
+
}
+
 
+
100% {
+
top: 0;
+
opacity: 1;
+
}
+
}
+
 
+
@-ms-keyframes reveal-header {
+
0% {
+
top: -4em;
+
opacity: 0;
+
}
+
 
+
100% {
+
top: 0;
+
opacity: 1;
+
}
+
}
+
 
+
@keyframes reveal-header {
+
0% {
+
top: -4em;
+
opacity: 0;
+
}
+
 
+
100% {
+
top: 0;
+
opacity: 1;
+
}
+
}
+
 
+
#header {
+
background: rgba(0, 0, 0, 0.975);
+
color: #a6a6a6;
+
cursor: default;
+
height: 3.25em;
+
left: 0;
+
line-height: 3.25em;
+
position: fixed;
+
text-align: right;
+
top: 0;
+
width: 100%;
+
z-index: 10001;
+
}
+
 
+
#header > .logo {
+
display: inline-block;
+
height: inherit;
+
left: 1.25em;
+
line-height: inherit;
+
margin: 0;
+
padding: 0;
+
position: absolute;
+
top: 0;
+
}
+
 
+
#header > .logo a {
+
font-size: 1.25em;
+
color: #FFF;
+
text-decoration: none;
+
}
+
 
+
#header > .logo a:hover {
+
color: rgba(255, 255, 255, 0.65);
+
}
+
 
+
#header > .logo span {
+
font-weight: 400;
+
font-size: .8em;
+
color: rgba(255, 255, 255, 0.65);
+
}
+
 
+
#header > a {
+
-moz-transition: color 0.2s ease-in-out;
+
-webkit-transition: color 0.2s ease-in-out;
+
-ms-transition: color 0.2s ease-in-out;
+
transition: color 0.2s ease-in-out;
+
display: inline-block;
+
padding: 0 0.75em;
+
color: inherit;
+
text-decoration: none;
+
color: #FFF;
+
}
+
 
+
#header > a:hover {
+
color: #f2f2f2;
+
}
+
 
+
#header > a[href="#menu"] {
+
text-decoration: none;
+
-webkit-tap-highlight-color: transparent;
+
}
+
 
+
#header > a[href="#menu"]:before {
+
content: "";
+
-moz-osx-font-smoothing: grayscale;
+
-webkit-font-smoothing: antialiased;
+
font-family: FontAwesome;
+
font-style: normal;
+
font-weight: normal;
+
text-transform: none !important;
+
}
+
 
+
#header > a[href="#menu"]:before {
+
margin: 0 0.5em 0 0;
+
}
+
 
+
#header > a + a[href="#menu"]:last-child {
+
border-left: solid 1px rgba(0, 0, 0, 0.15);
+
padding-left: 1.25em;
+
margin-left: 0.5em;
+
}
+
 
+
#header > a:last-child {
+
padding-right: 1.25em;
+
}
+
 
+
@media screen and (max-width: 736px) {
+
 
+
#header > a {
+
padding: 0 0.5em;
+
}
+
 
+
#header > a + a[href="#menu"]:last-child {
+
padding-left: 1em;
+
margin-left: 0.25em;
+
}
+
 
+
#header > a:last-child {
+
padding-right: 1em;
+
}
+
 
+
}
+
 
+
#header.reveal {
+
-moz-animation: reveal-header 0.5s ease;
+
-webkit-animation: reveal-header 0.5s ease;
+
-ms-animation: reveal-header 0.5s ease;
+
animation: reveal-header 0.5s ease;
+
}
+
 
+
#header.alt {
+
-moz-animation: none;
+
-webkit-animation: none;
+
-ms-animation: none;
+
animation: none;
+
background-color: transparent;
+
box-shadow: none;
+
overflow: hidden;
+
position: absolute;
+
top: 1.5em;
+
}
+
 
+
#header.alt h1 {
+
left: 2.5em;
+
}
+
 
+
#header.alt nav {
+
right: 2.5em;
+
}
+
 
+
@media screen and (max-width: 980px) {
+
 
+
body.subpage {
+
padding-top: 44px;
+
}
+
 
+
#header {
+
height: 44px;
+
line-height: 44px;
+
}
+
 
+
#header > h1 {
+
left: 1em;
+
}
+
 
+
#header > h1 a {
+
font-size: 1em;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 480px) {
+
 
+
#header {
+
min-width: 320px;
+
}
+
 
+
}
+
 
+
/* Menu */
+
 
+
#menu {
+
-moz-transform: translateX(20rem);
+
-webkit-transform: translateX(20rem);
+
-ms-transform: translateX(20rem);
+
transform: translateX(20rem);
+
-moz-transition: -moz-transform 0.5s ease, box-shadow 0.5s ease, visibility 0.5s;
+
-webkit-transition: -webkit-transform 0.5s ease, box-shadow 0.5s ease, visibility 0.5s;
+
-ms-transition: -ms-transform 0.5s ease, box-shadow 0.5s ease, visibility 0.5s;
+
transition: transform 0.5s ease, box-shadow 0.5s ease, visibility 0.5s;
+
-webkit-overflow-scrolling: touch;
+
background: rgba(0, 0, 0, 0.95);
+
box-shadow: none;
+
color: #000;
+
height: 100%;
+
max-width: 80%;
+
overflow-y: auto;
+
padding: 3rem 2rem;
+
position: fixed;
+
right: 0;
+
top: 0;
+
visibility: hidden;
+
width: 20rem;
+
z-index: 10002;
+
}
+
 
+
#menu > ul {
+
margin: 0 0 1rem 0;
+
}
+
 
+
#menu > ul.links {
+
list-style: none;
+
padding: 0;
+
}
+
 
+
#menu > ul.links > li {
+
padding: 0;
+
}
+
 
+
#menu > ul.links > li > a:not(.button) {
+
border: 0;
+
border-top: solid 1px rgba(255, 255, 255, 0.125);
+
color: rgba(255, 255, 255, 0.5);
+
display: block;
+
line-height: 3.5rem;
+
text-decoration: none;
+
text-transform: uppercase;
+
}
+
 
+
#menu > ul.links > li > a:not(.button):hover {
+
color: #FFF;
+
}
+
 
+
#menu > ul.links > li > .button {
+
display: block;
+
margin: 0.5rem 0 0 0;
+
}
+
 
+
#menu > ul.links > li:first-child > a:not(.button) {
+
border-top: 0 !important;
+
}
+
 
+
#menu .close {
+
text-decoration: none;
+
-moz-transition: color 0.2s ease-in-out;
+
-webkit-transition: color 0.2s ease-in-out;
+
-ms-transition: color 0.2s ease-in-out;
+
transition: color 0.2s ease-in-out;
+
-webkit-tap-highlight-color: transparent;
+
border: 0;
+
color: #999999;
+
cursor: pointer;
+
display: block;
+
height: 3.25rem;
+
line-height: 3.25rem;
+
padding-right: 1.25rem;
+
position: absolute;
+
right: 0;
+
text-align: right;
+
top: 0;
+
vertical-align: middle;
+
width: 7rem;
+
}
+
 
+
#menu .close:before {
+
-moz-osx-font-smoothing: grayscale;
+
-webkit-font-smoothing: antialiased;
+
font-family: FontAwesome;
+
font-style: normal;
+
font-weight: normal;
+
text-transform: none !important;
+
}
+
 
+
#menu .close:before {
+
content: '\f00d';
+
font-size: 1.25rem;
+
}
+
 
+
#menu .close:hover {
+
color: #000;
+
}
+
 
+
@media screen and (max-width: 736px) {
+
 
+
#menu .close {
+
height: 4rem;
+
line-height: 4rem;
+
}
+
 
+
}
+
 
+
#menu.visible {
+
-moz-transform: translateX(0);
+
-webkit-transform: translateX(0);
+
-ms-transform: translateX(0);
+
transform: translateX(0);
+
box-shadow: 0 0 1.5rem 0 rgba(0, 0, 0, 0.2);
+
visibility: visible;
+
}
+
 
+
@media screen and (max-width: 736px) {
+
 
+
#menu {
+
padding: 2.5rem 1.75rem;
+
}
+
 
+
}
+
 
+
/* Banner */
+
 
+
.banner {
+
background-color: #8a4680;
+
color: #e2d1df;
+
padding: 8em 0;
+
position: relative;
+
}
+
 
+
.banner input, .banner select, .banner textarea {
+
color: #ffffff;
+
}
+
 
+
.banner a {
+
color: #ffffff;
+
}
+
 
+
.banner strong, .banner b {
+
color: #ffffff;
+
}
+
 
+
.banner h1, .banner h2, .banner h3, .banner h4, .banner h5, .banner h6 {
+
color: #ffffff;
+
}
+
 
+
.banner blockquote {
+
border-left-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.banner code {
+
background: rgba(255, 255, 255, 0.075);
+
border-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.banner hr {
+
border-bottom-color: rgba(255, 255, 255, 0.25);
+
}
+
 
+
.banner.full {
+
padding: 0;
+
min-height: 100vh;
+
height: 100vh !important;
+
}
+
 
+
.banner.half {
+
padding: 0;
+
min-height: 50vh;
+
height: 50vh !important;
+
}
+
 
+
.banner:after {
+
-moz-pointer-events: none;
+
-webkit-pointer-events: none;
+
-ms-pointer-events: none;
+
pointer-events: none;
+
-moz-transition: opacity 1.5s ease-in-out, visibility 1.5s;
+
-webkit-transition: opacity 1.5s ease-in-out, visibility 1.5s;
+
-ms-transition: opacity 1.5s ease-in-out, visibility 1.5s;
+
transition: opacity 1.5s ease-in-out, visibility 1.5s;
+
background: #000000;
+
content: '';
+
display: block;
+
height: 100%;
+
left: 0;
+
opacity: 0;
+
position: absolute;
+
top: 0;
+
visibility: hidden;
+
width: 100%;
+
z-index: 2;
+
}
+
 
+
.banner .indicators {
+
bottom: 1.5em;
+
left: 0;
+
list-style: none;
+
margin: 0;
+
padding: 0;
+
position: absolute;
+
text-align: center;
+
width: 100%;
+
z-index: 2;
+
}
+
 
+
.banner .indicators li {
+
cursor: pointer;
+
display: inline-block;
+
height: 2em;
+
overflow: hidden;
+
padding: 0;
+
position: relative;
+
text-indent: 2em;
+
width: 2em;
+
}
+
 
+
.banner .indicators li:before {
+
background: rgba(255, 255, 255, 0.35);
+
border-radius: 100%;
+
content: '';
+
display: inline-block;
+
height: 0.8em;
+
left: 50%;
+
margin: -0.4em 0 0 -0.4em;
+
position: absolute;
+
text-indent: 0;
+
top: 50%;
+
width: 0.8em;
+
}
+
 
+
.banner .indicators li.visible:before {
+
background: #fff;
+
}
+
 
+
.banner > article {
+
-moz-transition: opacity 1.5s ease, visibility 1.5s;
+
-webkit-transition: opacity 1.5s ease, visibility 1.5s;
+
-ms-transition: opacity 1.5s ease, visibility 1.5s;
+
transition: opacity 1.5s ease, visibility 1.5s;
+
background-attachment: fixed;
+
background-position: center;
+
background-repeat: no-repeat;
+
background-size: cover;
+
height: 100%;
+
left: 0;
+
opacity: 0;
+
position: absolute;
+
text-align: center;
+
top: 0;
+
visibility: hidden;
+
width: 100%;
+
z-index: 0;
+
}
+
 
+
.banner > article:before {
+
content: '';
+
display: inline-block;
+
height: 100%;
+
vertical-align: middle;
+
}
+
 
+
.banner > article:after {
+
content: '';
+
display: block;
+
width: 100%;
+
height: 100%;
+
position: absolute;
+
top: 0;
+
left: 0;
+
background: #000;
+
opacity: 0.35;
+
}
+
 
+
.banner > article .inner {
+
position: relative;
+
display: inline-block;
+
vertical-align: middle;
+
z-index: 1;
+
}
+
 
+
.banner > article .inner > :last-child {
+
margin-bottom: 0;
+
}
+
 
+
.banner > article h2 {
+
font-size: 7rem;
+
margin-bottom: 0;
+
color: #FFF;
+
font-weight: 300;
+
}
+
 
+
.banner > article h2:after {
+
display: none;
+
}
+
 
+
.banner > article p {
+
color: rgba(255, 255, 255, 0.65);
+
text-transform: uppercase;
+
font-size: 1rem;
+
font-weight: 300;
+
margin: 0;
+
padding-bottom: 1.75rem;
+
letter-spacing: .25rem;
+
}
+
 
+
.banner > article p:after {
+
content: '';
+
position: absolute;
+
margin: auto;
+
right: 0;
+
bottom: 0;
+
left: 0;
+
width: 50%;
+
height: 1px;
+
background-color: rgba(255, 255, 255, 0.65);
+
}
+
 
+
.banner > article a {
+
color: #FFF;
+
text-decoration: none;
+
}
+
 
+
.banner > article img {
+
display: none;
+
}
+
 
+
.banner > article.visible {
+
opacity: 1;
+
visibility: visible;
+
}
+
 
+
.banner > article.top {
+
z-index: 1;
+
}
+
 
+
.banner > article.instant {
+
-moz-transition: none !important;
+
-webkit-transition: none !important;
+
-ms-transition: none !important;
+
transition: none !important;
+
}
+
 
+
body.is-loading .banner:after {
+
opacity: 1.0;
+
visibility: visible;
+
}
+
 
+
@media screen and (max-width: 1280px) {
+
 
+
.banner.full {
+
padding: 0;
+
min-height: 75vh;
+
height: 75vh !important;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 980px) {
+
 
+
.banner.full {
+
padding: 0;
+
min-height: 50vh;
+
height: 50vh !important;
+
}
+
 
+
.banner > article {
+
background-attachment: scroll;
+
}
+
 
+
}
+
 
+
@media screen and (max-width: 736px) {
+
 
+
.banner > article .inner {
+
width: 90%;
+
}
+
 
+
.banner > article p {
+
margin-bottom: 1rem;
+
}
+
 
+
.banner > article h2 {
+
font-size: 4em;
+
}
+
 
+
}
+
 
+
body.is-mobile .banner > article {
+
background-attachment: scroll;
+
}
+
 
+
/* Main */
+
 
+
#main {
+
padding: 4rem 0 2rem 0 ;
+
}
+
 
+
@media screen and (max-width: 736px) {
+
 
+
#main {
+
padding: 3rem 0 1rem 0 ;
+
}
+
 
+
}
+
 
+
/* Footer */
+
 
+
#footer {
+
padding: 2rem 0 2rem 0 ;
+
background: #000;
+
text-align: center;
+
}
+
 
+
#footer a {
+
color: rgba(255, 255, 255, 0.5);
+
}
+
 
+
#footer a:hover {
+
color: #FFF;
+
}
+
 
+
#footer .copyright {
+
color: #bbb;
+
font-size: 0.9rem;
+
margin: 0 0 2rem 0;
+
padding: 0;
+
text-align: center;
+
}
+
 
+
@media screen and (max-width: 736px) {
+
 
+
#footer {
+
padding: 3rem 0 1rem 0 ;
+
}
+
 
+
}
+
</style>
+
</head>
+
<body>
+
 
+
<!-- Header -->
+
<!-- Navbar -->
+
<!-- Navbar -->
+
<div class="w3-top" style="z-index: 4">
+
  <div class="w3-bar w3-black w3-card-2 w3-left-align w3-large">
+
<a class="w3-bar-item w3-button w3-hide-medium w3-hide-large w3-right w3-padding-large w3-hover-white w3-large w3-red" href="javascript:void(0);" onclick="myFunction()" title="Toggle Navigation Menu"><i class="fa fa-bars"></i></a>
+
<a href="https://2017.igem.org/Team:Mingdao" class="w3-bar-item w3-button w3-padding-large w3-white">Home</a>
+
<a href="https://2017.igem.org/Team:Mingdao/Achievements" class="w3-bar-item w3-button w3-hide-small w3-padding-large w3-blue w3-hover-white" style="text-decoration: none; color: white">Achievements</a>
+
<a href="https://2017.igem.org/Team:Mingdao/Demonstrate" class="w3-bar-item w3-button w3-hide-small w3-padding-large w3-hover-white" style="text-decoration: none; color: white">Project</a>
+
<a href="https://2017.igem.org/Team:Mingdao/HP/Silver" class="w3-bar-item w3-button w3-hide-small w3-padding-large w3-hover-white" style="text-decoration: none; color: white">Human-Practice</a>
+
<a href="#" class="w3-bar-item w3-button w3-hide-small w3-padding-large w3-hover-white" style="text-decoration: none; color: white">Notebook</a>
+
<a href="https://2017.igem.org/Team:Mingdao/Attributions" class="w3-bar-item w3-button w3-hide-small w3-padding-large w3-hover-white" style="text-decoration: none; color: white;">Attributions</a>
+
  </div>
+
</div>
+
<!-- Navbar END -->
+
 
+
 
+
 
+
<!-- Sidebar/menu -->
+
<nav class="w3-sidebar w3-collapse w3-animate-left" style="z-index:3;width:180px; background-color: #F8F9FA" id="mySidebar"><br>
+
 
+
    <div class="w3-container">
+
        <h5>Dashboard</h5>
+
    </div>
+
 
+
    <div class="w3-bar-block">
+
        <form action="https://2017.igem.org/Team:Mingdao/Collaborations" style="margin: 10px 15px -10px 0;">
+
            <button type="submit" class="btn btn-primary btn-block text-white" style="text-align: left; padding-left:1rem;font-size: 1.2rem;"> <i class="fa fa-bullseye fa-fw"></i>  Collaborate</button>
+
        </form>
+
        <form action="https://2017.igem.org/Team:Mingdao/HP/Silver" style="margin: 0 15px -10px 0;">
+
            <button type="submit" class="btn btn-primary btn-block text-white" style="text-align: left; padding-left:1rem;font-size: 1.2rem"> <i class="fa fa-bullseye fa-fw"></i> Silver</button>
+
        </form>
+
        <form action="https://2017.igem.org/Team:Mingdao/HP/Gold_Integrated" style="margin: 0 15px -20px 0;">
+
            <button type="submit" class="btn btn-primary btn-block text-white" style="text-align: left; padding-left:1rem;font-size: 1.2rem"> <i class="fa fa-bullseye fa-fw"></i> Gold</button>
+
        </form>
+
        <hr>
+
    </div>
+
 
+
  <div class="w3-bar-block">
+
    <a href="#" class="w3-bar-item w3-button w3-padding-16 w3-hide-large w3-dark-grey w3-hover-black" onclick="w3_close()" title="close menu"><i class="fa fa-remove fa-fw"></i>  Close Menu</a>
+
  </div>
+
    <nav class="navbar navbar-light bg-light">
+
      <a class="navbar-brand btn" href="#intro-md" style="text-decoration: none;">Intro</a>
+
      <a class="navbar-brand btn" href="#research-md" style="text-decoration: none">Mingdao</a>
+
      <a class="navbar-brand btn" href="#design-md" style="text-decoration: none">CSMU-NCHU</a>
+
    </nav>
+
</nav>
+
<!-- Overlay effect when opening sidebar on small screens -->
+
<div class="w3-overlay w3-hide-large w3-animate-opacity" onclick="w3_close()" style="cursor:pointer" title="close side menu" id="myOverlay"></div>
+
 
+
<div style="margin-left: 180px; background-color: #f2f2f2">
+
        <!-- Jumbotron -->
+
        <div class="jumbotron" style="margin-top: 1rem;">
+
          <h1 class="display-3"> &nbsp; </h1>
+
          <h1 class="display-3"> Collaboration </h1>
+
          <hr class="my-4">
+
 
+
        </div>
+
<!-- One -->
+
        <section id="intro" style="margin-bottom: 10rem">
+
            <div class="row">
+
                <div class="col-md-10 col-sm-12" style="display: block; margin: auto">
+
                    <img src="https://static.igem.org/mediawiki/2017/d/dd/Mingdaophil1026-21.png" width="78%" style="display: block; margin: auto">
+
                </div>
+
                <div class="col-md-7 col-sm-10 w3-padding-32" style="display: block; margin: auto">
+
                    <h4 style="font-size: 20px">
+
                        This year, we are very happy to collaborate with a collegiate iGEM team, CSMU_NCHU_Taiwan. We are both from Taichung City in Taiwan and the distance between us actually are very close, that is, not far from 5km.
+
                    </h4>
+
                    <h4 style="font-size: 20px">
+
                        In the meetup, CSMU_NCHU_Taiwan knew our project design and the problem to get from IDT the synthetic gene expression vector carrying glucose transporter genes of Salmonella. They advised that we can try to clone the gene from the bacteria and then assemble with the promoter. However, in safety issue, Salmonella is recognized as Biosafety Level 2 organism, they helped us to contact a lab PI, Dr. Cheng-Yang Huang, in Chung Shan Medical University who has handled the bacteria. Luckily, the genomic DNAs of Salmonella has been prepared for their previous research and kept some stored in the fridge. CSMU_NCHU_Taiwan amplified the gene fragments we want by PCR using our designed primers.
+
                    </h4>
+
                    <h4 style="font-size: 20px">
+
                        And in the other way, we did them a favor by subcloning BioBrick parts. We have good training in the skills of gene cloning learned from our instructor, Dr. Phil Chen. We have actually completed total at least 28 basic and composite BioBrick parts this year. And due to the limited remaining time of iGEM competition, CSMU_NCHU_Taiwan sought help from us to subclone parts from the synthetic genes made by IDT.
+
                    </h4>
+
                </div>
+
            </div>
+
            <!-- MINGDAO -->
+
            <div class="row">
+
                <div class="col-md-8 col-sm-10" style="display: block; margin: auto">
+
                        <div class="card">
+
                          <h4 class="card-header text-center" style="letter-spacing: 0.2rem; font-size:1.5rem; background-color: #004480; color: white">MINGDAO</h4>
+
                          <div class="card-body">
+
                            <h4 class="card-title w3-padding-32"> We helped CSMU_NCHU_Taiwan subclone one basic part of “F420-Dependent Glucose-6-phosphate Dehydrogenase” and one composite part of “T7 promoter & Lac operator and RBS from PET-29a”. We took two tubes containing DNA powder made by IDT from CSMU_NCHU_Taiwan. We dissolved the DNA and re-transformed E. coli DH5α with them. Then the two plasmids extracted from the bacteria have been cut by XbaI and PstI as inserts, and so did the pSB1C3 as a vector (Fig 1.). E. coli DH5α competent cells were transformed with the ligation products. The transformed E. coli  colonies were picked up and checked by colony PCR with VF2 and VR primers (Fig 2.). And one of these from each group were further checked with restriction enzymes of EcoRI and PstI (Fig 3.). Finally before giving back to CSMU_NCHUI_Taiwan, the plasmids were confirmed by DNA sequencing. In addition, we drew the plasmid map in VectorNTI format for them.</h4>
+
                            <div class="row">
+
                                <div class="col-md-6 col-sm-10">
+
                                    <img src="https://static.igem.org/mediawiki/2017/4/43/Mingdaophil1026-20.png" style="display: block; margin: auto">
+
                                </div>
+
                                <div class="col-md-7 col-sm-10">
+
                                    <h4><b>Fig 1. The plasmids were cut by XbaI and PstI. Lane 1: pSB1C3 (2044 bp+ 1095 bp). Lane 2: F420-Dependent Glucose-6-phosphate Dehydrogenase/pUCIDT (2769 bp+ 1032 bp). Lane 3: T7 promoter &amp; Lac operator and RBS from PET-29a/pIDTSMART(Amp) (2073 bp+109 bp)</b></h4>
+
                                </div>
+
                            </div>
+
                            <div class="row">
+
                                <div class="col-md-7 col-sm-10">
+
                                    <div class="row">
+
                                        <div class="col-md-6 col-sm-12" style="margin-top: 2rem">
+
                                                <img src="https://static.igem.org/mediawiki/2017/b/b3/Mingdaochuck1018-4.png">
+
                                        </div>
+
                                        <div class="col-md-6 col-sm-12" style="margin-top: 2rem">
+
                                                <img src="https://static.igem.org/mediawiki/2017/7/7d/Mingdaochuck1018-5.png">
+
                                        </div>
+
                                    </div>
+
                                    <div class="row">
+
                                        <div class="col-md-12 col-sm-12 w3-padding-24"><b>
+
                                            Fig 2. The results of colony PCR with VF2 and VR primers on the transformed colonies. Left gel: F420-Dependent Glucose-6-phosphate Dehydrogenase/pSB1C3 (1320 bp); Right gel: T7 promoter &amp; Lac operator and RBS from PET-29a/pSB1C3 (397 bp)  </b>
+
                                        </div>
+
                                    </div>
+
                                </div>
+
                                <div class="col-md-5 col-sm-10">
+
                                    <div class="row">
+
                                        <div class="col-md-12 col-sm-12">
+
                                            <img src="https://static.igem.org/mediawiki/2017/0/02/Mingdaochuck1018-6.png" width="35%" style="display: block; margin: auto">
+
                                        </div>
+
                                        <div class="col-md-12 col-sm-12 w3-padding-24" style="padding-left: 2rem; padding-right: 2rem; text-align: center">
+
                                        <b>
+
                                            Fig 3. Restriction enzyme digestion check by EcoRI and PstI for plasmids extracted from the transformed E. coli which have been confirmed by colony PCR
+
                                        </b>
+
                                        </div>
+
                                    </div>
+
                                </div>
+
                            </div>
+
                            <div class="row">
+
                                <div class="col-md-12 col-sm-12">
+
                                    <img src="https://static.igem.org/mediawiki/2017/a/af/Mingdaochuck1018-7.png" width="100%">
+
                                    <h4><b style="">Fig 4. The map of F420-Dependent Glucose-6-phosphate Dehydrogenase/pSB1C3 in VectorNTI format</b></h4>
+
                                </div>
+
                                <div class="col-md-12 col-sm-12">
+
                                    <img src="https://static.igem.org/mediawiki/2017/4/42/Mingdaochuck1018-8.png" width="100%">
+
                                    <h4><b style="">Fig 5. The map of T7 promoter &amp; Lac operator and RBS from PET-29a/pSB1C3 in VectorNTI format  </b></h4>
+
                                </div>
+
                            </div>
+
  
                            <div class="row">
+
            // overview
                                <div class="col-md-3 col-sm-3">
+
            if(scroll_pos < d_intro_pos) {
                                    <img src="https://static.igem.org/mediawiki/2017/e/e3/Mingdaochuck1018-9.png" width="100%">
+
                $(".path-dot").css('background-color', '#fff')
                                </div>
+
                $("#d-overview-btn").css('background-color', '#385e66');
                                <div class="col-md-5 col-sm-5">
+
            // intro
                                    <img src="https://static.igem.org/mediawiki/2017/1/1d/Mingdaochuck1018-10.png" width="100%">
+
            } else if(scroll_pos < d_antidote_pos){
                                </div>
+
              if(scroll_pos >= d_intro_pos){
                                <div class="col-md-3 col-sm-3">
+
                $(".path-dot").css('background-color', '#fff')
                                    <img src="https://static.igem.org/mediawiki/2017/5/53/Mingdaochuck1018-11.png" width="100%">
+
                $("#d-intro-btn").css('background-color', '#385e66');}
                                </div>
+
                            </div>
+
  
                          </div>
+
            // antidote
                        </div>
+
             } else if(scroll_pos < d_detective_pos){
                </div>
+
              if(scroll_pos >= d_antidote_pos){
             </div>
+
                 $(".path-dot").css('background-color', '#fff')
            <!-- MINGDAO END -->
+
                $("#d-antidote-btn").css('background-color', '#385e66');}
            <!-- CSMU -->
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            }
            <div class="row">
+
                 <div class="col-md-8 col-sm-10" style="display: block; margin: auto; margin-top: 3rem">
+
                        <div class="card">
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                          <h4 class="card-header text-center" style="letter-spacing: 0.2rem; font-size:1.5rem; background-color: #004480; color: white">CSMU_NCHU_Taiwan</h4>
+
                          <div class="card-body">
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                            <h4 class="card-title w3-padding-32">  CSMU_NCHU_Taiwan helped us amplify the glucose transporter genes from gDNA of Salmonella typhimurium LT2. We designed the specific primers with or without RBS (B0034) sequence which are targeting to three transporter genes, crr, ptsG and STM1128, respectively. CSMU_NCHU_Taiwan took the primer sets and performed PCR for us. And they also did a positive control to check the integrity and identity of genomic DNA of Salmonella typhimurium LT2. The PCR-amplified DNA fragments were shown in the DNA agarose gel in Fig 6.</h4>
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                            <div class="row">
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                                <div class="col-md-7 col-sm-7">
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                                    <img src="https://static.igem.org/mediawiki/2017/b/b6/Mingdaochuck1018-13.jpeg" width="100%" style="border-radius: 3%">
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                                </div>
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                                <div class="col-md-4 col-sm-4">
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                                    <h4><b>Fig 6. The amplified DNA fragments of glucose transporter genes by PCR from gDNA of Salmonella typhimurium LT2. Lanes 1. crr (510 bp); 2. RBS-crr (528 bp); 3. ptsG (1434 bp); 4. RBS-ptsG (1452bp); 5. 1128 (1497 bp); 6. RBS-1128 (1515 bp); 7. Positive control: STM3098 (423 bp), Appl Environ Microbiol. 2006.</b></h4>
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                                </div>
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                            </div>
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                            <div class="row w3-padding-32">
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                                <div class="col-md-6 col-sm-6">
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                                    <img src="https://static.igem.org/mediawiki/2017/f/fe/Mingdaochuck1018-14.png" width="80%" style="border-radius: 3%; display: block; margin: auto">
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                                </div>
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                                <div class="col-md-6 col-sm-6">
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                                    <img src="https://static.igem.org/mediawiki/2017/f/fb/Mingdaochuck1018-15.png" width="100%"style="border-radius: 3%">
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                                </div>
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                            </div>
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                            <div class="row w3-padding-32">
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            //detective
                                <!-- REFERNCE -->
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            else if( scroll_pos >= d_detective_pos) {
                                <div class="col-md-12 col-sm-12" >
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                $(".path-dot").css('background-color', '#fff')
                                    <div style="margin-left: 20px; margin-top: 3rem">
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                $("#d-detective-btn").css('background-color', '#385e66');
                                        <!-- Box -->
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            }
                                        <div style="margin-right: 50px">
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        });
                                            <ul class="list-group">
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    });
                                              <li class="list-group-item w3-wide" style="text-align: center"><strong>- REFERENCE -</strong></li>
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                                              <li class="list-group-item" style="color: black;">1. &nbsp;&nbsp;&nbsp;&nbsp;Comparison of Salmonella enterica Serovar Typhimurium LT2 and Non-LT2 Salmonella Genomic Sequences, and Genotyping of Salmonellae by Using PCR. Appl Environ Microbiol. 2006;72(9): 6142–6151.</li>
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                                            </ul>
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  </script>
                                        </div>
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                                    </div>
+
                                    <!-- REFERNCE END -->
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                                </div>
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                          </div>
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                        </div>
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                </div>
+
            </div>
+
            <!-- CSMU END -->
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        </section>
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</div>
+
    <!-- BOOTSTRAP -->
+
                <!-- Optional JavaScript -->
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                <!-- jQuery first, then Popper.js, then Bootstrap JS -->
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              <script src="https://code.jquery.com/jquery-3.2.1.slim.min.js" integrity="sha384-KJ3o2DKtIkvYIK3UENzmM7KCkRr/rE9/Qpg6aAZGJwFDMVNA/GpGFF93hXpG5KkN" crossorigin="anonymous"></script>
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              <script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.11.0/umd/popper.min.js" integrity="sha384-b/U6ypiBEHpOf/4+1nzFpr53nxSS+GLCkfwBdFNTxtclqqenISfwAzpKaMNFNmj4" crossorigin="anonymous"></script>
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              <script src="https://maxcdn.bootstrapcdn.com/bootstrap/4.0.0-beta/js/bootstrap.min.js" integrity="sha384-h0AbiXch4ZDo7tp9hKZ4TsHbi047NrKGLO3SEJAg45jXxnGIfYzk4Si90RDIqNm1" crossorigin="anonymous"></script>
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  <!-- BOOTSTRAP -->
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</body>
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</html>
 
</html>
{{:Team:Mingdao/test9}}
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{{:Team:Mingdao/test6}}

Revision as of 07:40, 12 October 2018

Description

Experiment

Collaboration

This year, we collaborated with a collegiate team NCHU-Taichung iGEM 2018 from National Chung Hsing University, Taiwan. NCHU is just located nearby Mingdao High School (15 minutes distance by car). At very beginning of the journey in iGEM 2018, we met up with each other at NCHU and Mingdao’s laboratory and school campus. We helped them host the 6th Asian-Pacific Conference hold in August. We shared with each other many ideas and dealt with problems met in the project and experiments. In this moment, just several weeks before iGEM Jamboree, we’d like to say, “Thank you, Friends. Happy to meet you because of iGEM”


We helped iGEM team NCHU to verify the bacterial cell growth affected by their BioBrick devices. They want to utilize synthetic biology to recover an environment polluted with dioxin. They created BioBricks of HAD, TonB and TLCC. They prepared the transformed E. coli with BioBricks and growth media (KB medium). Go to our lab in Mingdao, we measured the OD values of the E. coli cultured overnight and added IPTG for the induction of the gene expression and completed the assay following their protocol and instructions.



EXPERIMENT BY MINGDAO

Measure OD600 values for E. coli with HAD, TonB or TLCC genes
Dilute to OD600=0.05 with KB medium
Culture at for 2-3 hours until OD values reach approximately 0.6
Add 1mM IPTG for gene induction
Set a kinetic mode for analysis in Microplate Reader (BioTeK Synergy H1)
Analyze the data with each group for 3 repeats and report to Team NCHU


RESULT

The figure showed TonB helped bacterial growth and further improved by HAD. However, TLCC seems some toxic effect on bacterial growth.


On the other hand, we want to demonstrate our pathogen testing system in real adult mosquitoes and understand the GFP reporter system driven by GAM1 promoter can be triggered by E. coli. We prepared the plasmids of GAM1-GFP-polyA /pSB1C3 and heat-killed E. coli. Team NCUH brought us to the Mosquito Lab at Entomology Department of National Chung Hsing University and helped us microinjected DNAs into the midgut of Aedes aegypti.



EXPERIMENT BY NCHU

Make microinjection needles by pulling glass capillary tubes
Anesthetize the adult mosquitoes on ice
Fill the needle with the DNAs in Cellfectin II reagent with E. coli or not
Inject the DNA by inserting into the thorax of the mosquito.
Recover the mosquito for 2 days


RESULT

The mosquito injected with DNA plus E. coli showed fluorescence signal in Gel Imaging System and Blue LED Box after 2 days of DNA microinjection.


Syringe

GFP System

Mosquito Immune Signaling

AMP System