Difference between revisions of "Team:Newcastle/Results/Chemotaxis/AgarGrowth"

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       <thead>
 
       <thead>
 
         <tr>
 
         <tr>
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           <th>Parameter</th>
+
           <th>Species (Strain)</th>
−
           <th>Value</th>
+
           <th>Colony Pigmentation</th>
−
           <th>Source</th>
+
           <th>Colony Morphology</th>
 +
          <th>Points of Interest</th>
 
         </tr>
 
         </tr>
 
       </thead>
 
       </thead>
 
       <tbody>
 
       <tbody>
 
         <tr>
 
         <tr>
−
           <td>Growth Rate <i>Herbaspirillum seropedicae</i></td>
+
           <td><i>Azorhizobium caulinodans</i> (ORS571)</td>
−
           <td>4*10<sup>-4</sup> fg per second</td>
+
           <td>White</td>
−
           <td>growth curves (link)</td>
+
          <td>Regular form, Typically raised, Entire margin</td>
 +
           <td>Colonies rarely grow to a measurable size when grown at 30˚c on YEB media after 24 hours</td>
 
            
 
            
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
−
           <td>Growth Rate <i>Azospirillum brasilense</i></td>
+
           <td><i>Azospirillum brasilense</i> (SP245)</td>
−
           <td>1.314*10<sup>-4</sup> fg per second</td>
+
           <td>Orange/Pink</td>
−
           <td>growth curves (link)</td>
+
          <td>Non-slimy, Regular and round form, Entire margins</td>
 +
           <td>Both immature and dead colonies lack the orange/pink pigment, Colonies wrinkle with age</td>
 
          
 
          
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
−
           <td>Naringenin concentration threshold</td>
+
           <td><i>Herbaspirillum seropedicae</i> (Z67)</td>
−
           <td>150 μM</td>
+
           <td>Cream/Light Green</td>
−
           <td>Experiment (link)</td>
+
           <td>Circular or Irregular form (occasionally rhizoid), Raised elevation, Shiny</td>
−
       
+
          <td>Colonies took on a different morphology depending on how the media was innoculated; stab-innoculation lead to rhizoid form while spreading leads to circular/irregular form</td>
 
         </tr>
 
         </tr>
−
        <tr>
 
−
          <td>Diameter, initial length of <i>Herbaspirillum seropedicae</i></td>
 
−
          <td>0.7μm, 1.5μm</td>
 
−
          <td>[2]</td>
 
−
       
 
−
        </tr>
 
−
        <tr>
 
−
          <td>Diameter, initial length of <i>Azospirillum brasilense</i></td>
 
−
          <td>0.5μm, 2.9 μm</td>
 
−
          <td>[3]</td>
 
 
        
 
        
−
        </tr>
 
−
<tr>
 
−
          <td>Diameter, initial length of <i>Pseudomonas sp.</i></td>
 
−
          <td>0.5μm, 1.5μm</td>
 
−
          <td>[4]</td>
 
−
     
 
−
        </tr>
 
−
      </tbody>
 
 
     </table>
 
     </table>
−
<font size="2">Table 1: Table of the parameters used to create the soil community model and the references.</font>
+
<font size="2">Table 1: Qualitative analysis of <i>Azorhizobium caulinodans</i>, <i>Azospirillum brasilense</i>, <i>Herbaspirillum seropedicae</i> colonies grown on solid media.</font>
  
 
         </div>
 
         </div>

Revision as of 11:02, 12 October 2018

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Model

Alternative Roots

Characterising Bacterial Growth on Solid Media

Rationale Results

Rationale

In order to understand chemotaxis on agar, it was important to understand how the selected bacteria (Azospirillum brasilense, Azorhizobium caulinodans and Herbaspirillum seropedicae) grow on solid media in standard laboratory conditions. This measure would serve as a control to which experimental plates would be compared to. Familiarisation of bacteria also allows identification of abnormal behaviour and contamination.

Experimental Approach

Each species was grown on 1% agar. The type of agar utilised was selected from DSMZ suggestions which states which media is appropriate for each species [1-3]. Overnight culture for each species were then spread on 3 individual agar plates which were labelled and incubated at the DSMZ suggested temperature for 24-48hours (depending on the species). More details about out experimental design can be found on the protocol page.

Results