Difference between revisions of "Team:TecCEM/Notebook"

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    <img src="https://static.igem.org/mediawiki/2018/a/aa/T--TecCEM--Cells.gif" alt="Cell Gif">
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    <h1>Notebook</h1>
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<h1>Notebook</h1>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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</div>
 
</div>
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<div class="clear"></div>
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<div class="wrapper">
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    <nav class="sidebar-index">
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        <div class="sidebar-header">
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            <h3>Project/ Notebook</h3>
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<div class="column two_thirds_size">
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        </div>
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<h3>What should this page have?</h3>
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        <a href="#notebookSubmenu" data-toggle="collapse" aria-expanded="false" data-offset="100" data-change="sidemenu">
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<ul>
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            <span data-change="el" class="d-inline-block open"></span>
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<li>Chronological notes of what your team is doing.</li>
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            Index
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<li> Brief descriptions of daily important events.</li>
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        </a>
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<li>Pictures of your progress. </li>
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        <ul class="collapse list-unstyled" id="notebookSubmenu">
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<li>Mention who participated in what task.</li>
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            <li class="active">
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</ul>
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                <a  data-target="#week18"> Week 18th</a>
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            </li>
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            <li>
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                <a  data-target="#week19"> Week 19th</a>
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            </li>
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        </ul>
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    </nav>
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    <div class="content">
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        <div class="container first-container" id="description">
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            <div class="row">
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                <div class="col">
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                    <div class="accordion" id="accordionNotebook">
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                        <div class="card">
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                            <div class="card-header" id="week18">
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                                <h5 class="mb-0">
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                                    <button class="btn btn-link" type="button" data-toggle="collapse" data-target="#collapseOne"
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                                        aria-expanded="true" aria-controls="collapseOne">
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                                        Week 18th
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                                    </button>
 +
                                    <strong>13 - 19 May.</strong>
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                                </h5>
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                            </div>
 +
                            <div id="collapseOne" class="collapse show" aria-labelledby="week18" data-parent="#accordionNotebook">
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                                <div class="card-body">
 +
                                    <h2 class="text-notebook">Summary</h2>
 +
                                    <p>Research for characterization began and sub-projects deadlines were assigned.
 +
                                        Logistics of the Latin American Meet-up began. A visit to Six Flags was made.</p>
 +
                                    <h3 class="text-human-practices">Wet lab</h3>
 +
                                    <p>Preparation of solutions to be used for the competent cells. Transformation
 +
                                        efficiency for E. coli DH5α and antibiotic viability were tested.
 +
                                    </p>
 +
                                    <h3 class="text-human-practices">Dry lab</h3>
 +
                                    <p>Delimitations of the mathematical model were made. Pitching of software ideas
 +
                                        and sub-projects. We started looking into the possibility of creating a
 +
                                        skinchip in a mini reactor. Investigation about the scaffold heterotrimer was
 +
                                        appointed. The development of biobricks for recombinant leptin and collagen was
 +
                                        started. A meeting with an expert in miRNA therapy was appointed, new insights
 +
                                        about anti-miRNA were established from that meeting and the viability was
 +
                                        researched. Leptin and collagen were codon optimized for their recombinant
 +
                                        production in Escherichia coli BL21 (DE3) and Rosetta (DE3) respectively.
 +
                                    </p>
 +
                                    <h3 class="text-human-practices">Human Practices</h3>
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                                    <p>Committee for human practices was delimited. A brainstorming of ideas was done
 +
                                        and the best ideas were picked up in order to develop them in the near future.
 +
                                    </p>
 +
                                </div>
 +
                            </div>
 +
                        </div>
 +
                        <div class="card">
 +
                            <div class="card-header" id="week19">
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                                <h5 class="mb-0">
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                                    <button class="btn btn-link" type="button" data-toggle="collapse" data-target="#collapseTwo"
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                                        aria-expanded="true" aria-controls="collapseTwo">
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                                        Week 19th
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                                    </button>
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                                    <strong>20 - 26 May.</strong>
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                                </h5>
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                            </div>
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                            <div id="collapseTwo" class="collapse" aria-labelledby="week19" data-parent="#accordionNotebook">
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                                <div class="card-body">
 +
                                    <h2 class="text-notebook">Summary</h2>
 +
                                    <p>Worklab began and we had meeting to assign new sub-project. Wiki page
 +
                                        planification began, we contacted a Graphic designer to help out. We also
 +
                                        contacted several specialists in tissue regeneration to gain insights about the
 +
                                        project. </p>
 +
                                    <h3 class="text-human-practices">Wet lab</h3>
 +
                                    <p>Lab got closed off for maintenance reasons. The iGEM kit got stuck at the
 +
                                        customs border protection.
 +
                                    </p>
 +
                                    <h3 class="text-human-practices">Dry lab</h3>
 +
                                    <p>Skinchip 3D model was designed and software ideas were pitched. Biobricks were
 +
                                        checked and revised. Chemical modifications for anti-miRNA were investigated,
 +
                                        locked nucleic acid (LNA) technology appointed as the best method for miRNA
 +
                                        silencing. Receptors within the scaffold for stem cell migration/call was
 +
                                        investigated but also other molecules apart from the main project
 +
                                        protein/molecules were investigated for the same purpose.
 +
                                    </p>
 +
                                    <h3 class="text-human-practices">Human Practices</h3>
 +
                                    <p>Fundraiser organization and planification, tasks were handed out. Went to a
 +
                                        public talk about tissue regeneration in burn victims using nanoparticles of
 +
                                        chitosan, we made contact with the people involved in the conference and
 +
                                        received a feedback, another meeting with the experts was appointed. We had a
 +
                                        meeting with a communication reporter to be published in a magazine. Online
 +
                                        meet up with iGEM Brazil, Ecuador and Chile were performed.
 +
                                    </p>
 +
                                </div>
 +
                            </div>
 +
                        </div>
 +
                    </div>
 +
                </div>
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            </div>
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        </div>
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    </div>
 
</div>
 
</div>
  
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<div class="column third_size">
 
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<div class="highlight decoration_A_full">
 
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<h3>Inspiration</h3>
 
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<p>You can see what others teams have done to organize their notes:</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
 
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
 
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
 
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</ul>
 
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</div>
 
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</div>
 
 
</html>
 
</html>
 
{{TecCEM/Footer}}
 
{{TecCEM/Footer}}

Revision as of 06:40, 13 October 2018

Cell Gif

Notebook

13 - 19 May.

Summary

Research for characterization began and sub-projects deadlines were assigned. Logistics of the Latin American Meet-up began. A visit to Six Flags was made.

Wet lab

Preparation of solutions to be used for the competent cells. Transformation efficiency for E. coli DH5α and antibiotic viability were tested.

Dry lab

Delimitations of the mathematical model were made. Pitching of software ideas and sub-projects. We started looking into the possibility of creating a skinchip in a mini reactor. Investigation about the scaffold heterotrimer was appointed. The development of biobricks for recombinant leptin and collagen was started. A meeting with an expert in miRNA therapy was appointed, new insights about anti-miRNA were established from that meeting and the viability was researched. Leptin and collagen were codon optimized for their recombinant production in Escherichia coli BL21 (DE3) and Rosetta (DE3) respectively.

Human Practices

Committee for human practices was delimited. A brainstorming of ideas was done and the best ideas were picked up in order to develop them in the near future.

20 - 26 May.

Summary

Worklab began and we had meeting to assign new sub-project. Wiki page planification began, we contacted a Graphic designer to help out. We also contacted several specialists in tissue regeneration to gain insights about the project.

Wet lab

Lab got closed off for maintenance reasons. The iGEM kit got stuck at the customs border protection.

Dry lab

Skinchip 3D model was designed and software ideas were pitched. Biobricks were checked and revised. Chemical modifications for anti-miRNA were investigated, locked nucleic acid (LNA) technology appointed as the best method for miRNA silencing. Receptors within the scaffold for stem cell migration/call was investigated but also other molecules apart from the main project protein/molecules were investigated for the same purpose.

Human Practices

Fundraiser organization and planification, tasks were handed out. Went to a public talk about tissue regeneration in burn victims using nanoparticles of chitosan, we made contact with the people involved in the conference and received a feedback, another meeting with the experts was appointed. We had a meeting with a communication reporter to be published in a magazine. Online meet up with iGEM Brazil, Ecuador and Chile were performed.