Difference between revisions of "Team:TUDelft/Primers/Main"

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   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
 
     <td>031</td>
 
     <td>031</td>
−
     <td></td>
+
     <td>VF2</td>
−
     <td></td>
+
     <td>General</td>
−
     <td></td>
+
     <td>tgccacctgacgtctaagaa</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
 
     <td>032</td>
 
     <td>032</td>
−
     <td></td>
+
     <td>VR</td>
−
     <td></td>
+
     <td>General</td>
−
     <td></td>
+
     <td>attaccgcctttgagtgagc</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
 
     <td>033</td>
 
     <td>033</td>
−
     <td></td>
+
     <td>fw_sgRNA_1</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>aagcTAATACGACTCACTATAGGGGAGGCCGAGAATATCA</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
 
     <td>034</td>
 
     <td>034</td>
−
     <td></td>
+
     <td>rv_sgRNA_1</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>AAAAGCACCGACTCGGTGCCACTT</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
 
     <td>035</td>
 
     <td>035</td>
−
     <td></td>
+
     <td>fw_sgRNA_2</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>aagcTAATACGACTCACTATAGGACAGCCCGTCGTGATATTCTGTTTTAGAGCTAGAAATAG</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
 
     <td>036</td>
 
     <td>036</td>
−
     <td></td>
+
     <td>fw_adTn5_1</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>CTAGCTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
 
     <td>037</td>
 
     <td>037</td>
−
     <td></td>
+
     <td>rv_adTn5_1</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAGCTAG</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
 
     <td>038</td>
 
     <td>038</td>
−
     <td></td>
+
     <td>fw_1_AUpart</td>
−
     <td></td>
+
     <td>d-AuNPs</td>
−
     <td></td>
+
     <td>GATCGAATTCGCGGCCGCTTC</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
 
     <td>039</td>
 
     <td>039</td>
−
     <td></td>
+
     <td>rv_1_AUpart</td>
−
     <td></td>
+
     <td>d-AuNPs</td>
−
     <td></td>
+
     <td>CGATTCTGCAGCGGCCGCTAC</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
 
     <td>040</td>
 
     <td>040</td>
−
     <td></td>
+
     <td>fw_2_AUpart</td>
−
     <td></td>
+
     <td>d-AuNPs</td>
−
     <td></td>
+
     <td>GGAGGCCGAGAATATCACGA</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
 
     <td>041</td>
 
     <td>041</td>
−
     <td></td>
+
     <td>rv_2_AUpart</td>
−
     <td></td>
+
     <td>d-AuNPs</td>
−
     <td></td>
+
     <td>TTGAGATCGTTTTGGTCTGC</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
 
     <td>042</td>
 
     <td>042</td>
−
     <td></td>
+
     <td>fw_3_AUpart</td>
−
     <td></td>
+
     <td>d-AuNPs</td>
−
     <td></td>
+
     <td>CAGGTCGACAAGCTTGCG</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
 
     <td>043</td>
 
     <td>043</td>
−
     <td></td>
+
     <td>fw_dCas9</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>atataGCGGCCGCtatggataagaaatactcaataggcttagc</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
 
     <td>044</td>
 
     <td>044</td>
−
     <td></td>
+
     <td>rv_dCas9</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>tatGGTACCttatGGCCGGCCgtcacctcctagctgactc</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
 
     <td>045</td>
 
     <td>045</td>
−
     <td></td>
+
     <td>fw_1_Tn5-L-dxCas9_Gblock</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>ACCATGGGCAGCAGC</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
 
     <td>046</td>
 
     <td>046</td>
−
     <td></td>
+
     <td>rv_1_Tn5-L-dxCas9_Gblock</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>GTAGGGTATCCGAAATGTGAGGATTTTC</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
 
     <td>047</td>
 
     <td>047</td>
−
     <td></td>
+
     <td>fw_2_Tn5-L-dxCas9_Gblock</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>GGGAAAAGATTGAGAAAATCCTCACATTTCG</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
 
     <td>048</td>
 
     <td>048</td>
−
     <td></td>
+
     <td>rv_2_Tn5-L-dxCas9_Gblock</td>
−
     <td></td>
+
     <td>Fusion Protein</td>
−
     <td></td>
+
     <td>TGGCGTTCAAATTTCGCAGC</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">

Revision as of 12:11, 13 October 2018

partssequences

stuff to write here

Primers

ID Name Module Sequence (5' to 3')
001 fw_dxCas9 Fusion Protein atataGCGGCCGCtatggacaagaagtactccat
002 rv_dxCas9 Fusion Protein tatGGTACCttatGGCCGGCCcaccttcctcttcttcttgg
003 fw_dxCas9_mut_EcoRI Fusion Protein P-gcgaattttccaaaagagtgatcc
004 rv_dxCas9_mut_EcoRI Fusion Protein P-ttatttgctcgatgatctcatcaagg
005 fw_Tn5_afterC Fusion Protein tataGGCCGGCCtAAACTGGGCGGCGGCGCGCCGGCGGTGGGCGGCGGCCCGAAAGCGGCGGATAAAatgattaccagtgcactgcatc
006 rv_Tn5_afterC Fusion Protein tataGGTACCttagattttaatgccctgcgcca
007 fw_Tn5_beforeC Fusion Protein
008 rv_Tn5_beforeC Fusion Protein tataGCGGCCGCTTTATCCGCCGCTTTCGGGCCGCCGCCCACCGCCGGCGCGCCGCCGCCCAGTTTgattttaatgccctgcgcca
009 rv_dxCas9_mut1_GA Fusion Protein cattactaaagatctcttgcaggtagca
010 fw_dxCas9_mut1_GA Fusion Protein gctacctgcaagagatctttagtaatg
011 rv_dxCas9_mut2_GA Fusion Protein gaccttaacggtttgcagtattcc
012 fw_dxCas9_mut2_GA Fusion Protein ggaatactgcaaaccgttaagg
013 rv_dxCas9_mut3_GA Fusion Protein ccgttttgcaggtagtacag
014 fw_dxCas9_mut3_GA Fusion Protein ctgtactacctgcaaaacgg
015 rv_dxCas9_mut4_GA Fusion Protein tcgttacctttttgcagcac
016 fw_dxCas9_mut4_GA Fusion Protein tgctgcaaaaaggtaacgag
017 rv_dxCas9_mut5_GA Fusion Protein tacttgaaggctgccggc
018 fw_dxCas9_mut_GA Fusion Protein gccggcagccttcaag
019 fw_dxCas9_mut1_BL Fusion Protein P-gctacctgcaagagatctttagtaatg
020 rv_dxCas9_mut1_BL Fusion Protein P-agatccgattctttctgcgg
021 fw_dxCas9_mut2_BL Fusion Protein P-ggaatactgcaaaccgttaagg
022 rv_dxCas9_mut2_BL Fusion Protein P-ctttttgatagctgggctacct
023 fw_dxCas9_mut3_BL Fusion Protein P-ctgtactacctgcaaaacgg
024 rv_dxCas9_mut3_BL Fusion Protein P-gtagagcttctcattctgaagc
025 fw_dxCas9_mut4_BL Fusion Protein P-ctgcaaaaaggtaacgagctg
026 rv_dxCas9_mut4_BL Fusion Protein P-cacgcccgcactagc
027 fw_dxCas9_mut5_BL Fusion Protein P-gccggcagccttcaag
028 rv_dxCas9_mut5_BL Fusion Protein P-gcgcccaagttggtca
029 fw_T7_promoter General TAATACGACTCACTATAGGG
030 rv_T7_promoter General GCTAGTTATTGCTCAGCGG
031 VF2 General tgccacctgacgtctaagaa
032 VR General attaccgcctttgagtgagc
033 fw_sgRNA_1 Fusion Protein aagcTAATACGACTCACTATAGGGGAGGCCGAGAATATCA
034 rv_sgRNA_1 Fusion Protein AAAAGCACCGACTCGGTGCCACTT
035 fw_sgRNA_2 Fusion Protein aagcTAATACGACTCACTATAGGACAGCCCGTCGTGATATTCTGTTTTAGAGCTAGAAATAG
036 fw_adTn5_1 Fusion Protein CTAGCTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC
037 rv_adTn5_1 Fusion Protein GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAGCTAG
038 fw_1_AUpart d-AuNPs GATCGAATTCGCGGCCGCTTC
039 rv_1_AUpart d-AuNPs CGATTCTGCAGCGGCCGCTAC
040 fw_2_AUpart d-AuNPs GGAGGCCGAGAATATCACGA
041 rv_2_AUpart d-AuNPs TTGAGATCGTTTTGGTCTGC
042 fw_3_AUpart d-AuNPs CAGGTCGACAAGCTTGCG
043 fw_dCas9 Fusion Protein atataGCGGCCGCtatggataagaaatactcaataggcttagc
044 rv_dCas9 Fusion Protein tatGGTACCttatGGCCGGCCgtcacctcctagctgactc
045 fw_1_Tn5-L-dxCas9_Gblock Fusion Protein ACCATGGGCAGCAGC
046 rv_1_Tn5-L-dxCas9_Gblock Fusion Protein GTAGGGTATCCGAAATGTGAGGATTTTC
047 fw_2_Tn5-L-dxCas9_Gblock Fusion Protein GGGAAAAGATTGAGAAAATCCTCACATTTCG
048 rv_2_Tn5-L-dxCas9_Gblock Fusion Protein TGGCGTTCAAATTTCGCAGC
049
050
051
052
052
053
054
055
056
057
058
059
060
061
062
063
064
065
066
067
068
069
070
071
072
073
074
075
076
077
078
079
080
081
082
083
084
085
086
087
088
089
090
091
092
093
094
095
096
097
098
099