Difference between revisions of "Team:TUDelft/Primers/Main"

Line 69: Line 69:
 
     <td>fw_Tn5_afterC</td>
 
     <td>fw_Tn5_afterC</td>
 
     <td>Fusion Protein</td>
 
     <td>Fusion Protein</td>
−
     <td style="word-break:break-all;">tataGGCCGGCCtAAACTGGGCGGCGGCGCGCCGGCGGTGGGCGGCGGCCCGAAAGCGGCGGATAAAatgattaccagtgcactgcatc</td>
+
     <td>tataGGCCGGCCtAAACTGGGCGGCGGCGCGCCGGCGGTGGGCGGCGGCCCGAAAGCGGCGGATAAAatgattaccagtgcactgcatc</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
Line 87: Line 87:
 
     <td>rv_Tn5_beforeC</td>
 
     <td>rv_Tn5_beforeC</td>
 
     <td>Fusion Protein</td>
 
     <td>Fusion Protein</td>
−
     <td style="word-break:break-all;">tataGCGGCCGCTTTATCCGCCGCTTTCGGGCCGCCGCCCACCGCCGGCGCGCCGCCGCCCAGTTTgattttaatgccctgcgcca</td>
+
     <td>tataGCGGCCGCTTTATCCGCCGCTTTCGGGCCGCCGCCCACCGCCGGCGCGCCGCCGCCCAGTTTgattttaatgccctgcgcca</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
Line 249: Line 249:
 
     <td>fw_sgRNA_2</td>
 
     <td>fw_sgRNA_2</td>
 
     <td>Fusion Protein</td>
 
     <td>Fusion Protein</td>
−
     <td style="word-break:break-all;">aagcTAATACGACTCACTATAGGACAGCCCGTCGTGATATTCTGTTTTAGAGCTAGAAATAG</td>
+
     <td>aagcTAATACGACTCACTATAGGACAGCCCGTCGTGATATTCTGTTTTAGAGCTAGAAATAG</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
Line 255: Line 255:
 
     <td>fw_adTn5_1</td>
 
     <td>fw_adTn5_1</td>
 
     <td>Fusion Protein</td>
 
     <td>Fusion Protein</td>
−
     <td style="word-break:break-all;">CTAGCTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC</td>
+
     <td>CTAGCTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
Line 363: Line 363:
 
     <td>fw_BB_Tn5</td>
 
     <td>fw_BB_Tn5</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
−
     <td style="word-break:break-all;">GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGatgattaccagtgcactgcatcg</td>
+
     <td>GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGatgattaccagtgcactgcatcg</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
Line 369: Line 369:
 
     <td>rv_BB_Tn5</td>
 
     <td>rv_BB_Tn5</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
−
     <td style="word-break:break-all;">CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAttagattttaatgccctgcgccatc</td>
+
     <td>CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAttagattttaatgccctgcgccatc</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
Line 375: Line 375:
 
     <td>fw_BB_dxCas9</td>
 
     <td>fw_BB_dxCas9</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
−
     <td style="word-break:break-all;">GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGatggacaagaagtactccattgg</td>
+
     <td>GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGatggacaagaagtactccattgg</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
Line 381: Line 381:
 
     <td>rv_BB_dxCas9</td>
 
     <td>rv_BB_dxCas9</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
−
     <td style="word-break:break-all;">CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAttatggccggcccac</td>
+
     <td>CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAttatggccggcccac</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
Line 387: Line 387:
 
     <td>fw_BB_Linker</td>
 
     <td>fw_BB_Linker</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
−
     <td style="word-break:break-all;">CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGCCGGCCtAAACTGG</td>
+
     <td>CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGCCGGCCtAAACTGG</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
Line 393: Line 393:
 
     <td>rv_BB_Linker</td>
 
     <td>rv_BB_Linker</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
−
     <td style="word-break:break-all;">CTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTTATCCGCCGCTTTCGGGCCGCCGC</td>
+
     <td>CTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTTATCCGCCGCTTTCGGGCCGCCGC</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
Line 399: Line 399:
 
     <td>fw_BB_T7p</td>
 
     <td>fw_BB_T7p</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
−
     <td style="word-break:break-all;">ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGGAATTGTG</td>
+
     <td>ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGGAATTGTG</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
Line 405: Line 405:
 
     <td>rv_BB_T7p</td>
 
     <td>rv_BB_T7p</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
−
     <td style="word-break:break-all;">TTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAGCTAGTTATTGCTCAGCGGTG</td>
+
     <td>TTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAGCTAGTTATTGCTCAGCGGTG</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
Line 411: Line 411:
 
     <td>fw_adTn5_2</td>
 
     <td>fw_adTn5_2</td>
 
     <td>Fusion Protein</td>
 
     <td>Fusion Protein</td>
−
     <td style="word-break:break-all;">CTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC</td>
+
     <td>CTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">
Line 417: Line 417:
 
     <td>rv_adTn5_2</td>
 
     <td>rv_adTn5_2</td>
 
     <td>Fusion Protein</td>
 
     <td>Fusion Protein</td>
−
     <td style="word-break:break-all;">GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAG</td>
+
     <td>GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAG</td>
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
Line 423: Line 423:
 
     <td>rv_adTn5_label</td>
 
     <td>rv_adTn5_label</td>
 
     <td>Fusion Protein</td>
 
     <td>Fusion Protein</td>
−
     <td style="word-break:break-all;">Cy-GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAG</td>
+
     <td>Cy-GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAG</td>
 
   </tr>
 
   </tr>
 
<tr class="tableunevengrnbl">
 
<tr class="tableunevengrnbl">

Revision as of 13:34, 13 October 2018

partssequences

stuff to write here

Primers

ID Name Module Sequence (5' to 3')
001 fw_dxCas9 Fusion Protein atataGCGGCCGCtatggacaagaagtactccat
002 rv_dxCas9 Fusion Protein tatGGTACCttatGGCCGGCCcaccttcctcttcttcttgg
003 fw_dxCas9_mut_EcoRI Fusion Protein P-gcgaattttccaaaagagtgatcc
004 rv_dxCas9_mut_EcoRI Fusion Protein P-ttatttgctcgatgatctcatcaagg
005 fw_Tn5_afterC Fusion Protein tataGGCCGGCCtAAACTGGGCGGCGGCGCGCCGGCGGTGGGCGGCGGCCCGAAAGCGGCGGATAAAatgattaccagtgcactgcatc
006 rv_Tn5_afterC Fusion Protein tataGGTACCttagattttaatgccctgcgcca
007 fw_Tn5_beforeC Fusion Protein tataGAATTCtatgattaccagtgcactgcatc
008 rv_Tn5_beforeC Fusion Protein tataGCGGCCGCTTTATCCGCCGCTTTCGGGCCGCCGCCCACCGCCGGCGCGCCGCCGCCCAGTTTgattttaatgccctgcgcca
009 rv_dxCas9_mut1_GA Fusion Protein cattactaaagatctcttgcaggtagca
010 fw_dxCas9_mut1_GA Fusion Protein gctacctgcaagagatctttagtaatg
011 rv_dxCas9_mut2_GA Fusion Protein gaccttaacggtttgcagtattcc
012 fw_dxCas9_mut2_GA Fusion Protein ggaatactgcaaaccgttaagg
013 rv_dxCas9_mut3_GA Fusion Protein ccgttttgcaggtagtacag
014 fw_dxCas9_mut3_GA Fusion Protein ctgtactacctgcaaaacgg
015 rv_dxCas9_mut4_GA Fusion Protein tcgttacctttttgcagcac
016 fw_dxCas9_mut4_GA Fusion Protein tgctgcaaaaaggtaacgag
017 rv_dxCas9_mut5_GA Fusion Protein tacttgaaggctgccggc
018 fw_dxCas9_mut_GA Fusion Protein gccggcagccttcaag
019 fw_dxCas9_mut1_BL Fusion Protein P-gctacctgcaagagatctttagtaatg
020 rv_dxCas9_mut1_BL Fusion Protein P-agatccgattctttctgcgg
021 fw_dxCas9_mut2_BL Fusion Protein P-ggaatactgcaaaccgttaagg
022 rv_dxCas9_mut2_BL Fusion Protein P-ctttttgatagctgggctacct
023 fw_dxCas9_mut3_BL Fusion Protein P-ctgtactacctgcaaaacgg
024 rv_dxCas9_mut3_BL Fusion Protein P-gtagagcttctcattctgaagc
025 fw_dxCas9_mut4_BL Fusion Protein P-ctgcaaaaaggtaacgagctg
026 rv_dxCas9_mut4_BL Fusion Protein P-cacgcccgcactagc
027 fw_dxCas9_mut5_BL Fusion Protein P-gccggcagccttcaag
028 rv_dxCas9_mut5_BL Fusion Protein P-gcgcccaagttggtca
029 fw_T7_promoter General TAATACGACTCACTATAGGG
030 rv_T7_promoter General GCTAGTTATTGCTCAGCGG
031 VF2 General tgccacctgacgtctaagaa
032 VR General attaccgcctttgagtgagc
033 fw_sgRNA_1 Fusion Protein aagcTAATACGACTCACTATAGGGGAGGCCGAGAATATCA
034 rv_sgRNA_1 Fusion Protein AAAAGCACCGACTCGGTGCCACTT
035 fw_sgRNA_2 Fusion Protein aagcTAATACGACTCACTATAGGACAGCCCGTCGTGATATTCTGTTTTAGAGCTAGAAATAG
036 fw_adTn5_1 Fusion Protein CTAGCTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC
037 rv_adTn5_1 Fusion Protein GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAGCTAG
038 fw_1_AUpart d-AuNPs GATCGAATTCGCGGCCGCTTC
039 rv_1_AUpart d-AuNPs CGATTCTGCAGCGGCCGCTAC
040 fw_2_AUpart d-AuNPs GGAGGCCGAGAATATCACGA
041 rv_2_AUpart d-AuNPs TTGAGATCGTTTTGGTCTGC
042 fw_3_AUpart d-AuNPs CAGGTCGACAAGCTTGCG
043 fw_dCas9 Fusion Protein atataGCGGCCGCtatggataagaaatactcaataggcttagc
044 rv_dCas9 Fusion Protein tatGGTACCttatGGCCGGCCgtcacctcctagctgactc
045 fw_1_Tn5-L-dxCas9_Gblock Fusion Protein ACCATGGGCAGCAGC
046 rv_1_Tn5-L-dxCas9_Gblock Fusion Protein GTAGGGTATCCGAAATGTGAGGATTTTC
047 fw_2_Tn5-L-dxCas9_Gblock Fusion Protein GGGAAAAGATTGAGAAAATCCTCACATTTCG
048 rv_2_Tn5-L-dxCas9_Gblock Fusion Protein TGGCGTTCAAATTTCGCAGC
049 fw_pACYC-Duet_Backbone Fusion Protein GGTACCCTCGAGTCTGG
050 rv_pACYC-Duet_Backbone Fusion Protein GAATTCGGATCCTGGCTG
051 fw_BB_pSB1C3 BioBrick TACTAGTAGCGGCCGCTGC
052 rv_BB_pSB1C3 BioBrick CTAGAAGCGGCCGCGA
053 rv_BB_pSB1C3_extraNuc BioBrick CTCTAGAAGCGGCCGCGA
054 fw_BB_Tn5 BioBrick GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGatgattaccagtgcactgcatcg
055 rv_BB_Tn5 BioBrick CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAttagattttaatgccctgcgccatc
056 fw_BB_dxCas9 BioBrick GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGatggacaagaagtactccattgg
057 rv_BB_dxCas9 BioBrick CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAttatggccggcccac
058 fw_BB_Linker BioBrick CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGCCGGCCtAAACTGG
059 rv_BB_Linker BioBrick CTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTTATCCGCCGCTTTCGGGCCGCCGC
060 fw_BB_T7p BioBrick ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGGAATTGTG
061 rv_BB_T7p BioBrick TTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAGCTAGTTATTGCTCAGCGGTG
062 fw_adTn5_2 Fusion Protein CTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC
063 rv_adTn5_2 Fusion Protein GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAG
064 rv_adTn5_label Fusion Protein Cy-GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAG
065 fw_1_AUpart d-AuNPs
066 fw_bovine_albumin Sample Preparation TTGTTTCAACTCAAACAGCCTT
067 rv_bovine_albumin Sample Preparation AGAATCCCCATGGACAGAGA
068 fw_vectorxcas_spike Sample Preparation AGTAGCGGCCGCTGCAGaaggaagaggctgcaatctgtggc
069 rv_vectorxcas_spike Sample Preparation AGAAGCGGCCGCGAATTCcacacaagcagggagcagatactgg
070 fw_epo_spike Sample Preparation tgctccctgcttgtgtgGAATTCGCGGCCGCTTCT
071 rv_epo_spike Sample Preparation gattgcagcctcttccttCTGCAGCGGCCGCTAC
072 fw_epo_frag Sample Preparation GCCTCCCAGTCCTGGGCG
073 rv_epo_frag Sample Preparation GCAGTTGCAGGGGCTCCCAC
074 fw_epo_check Sample Preparation CCTGGAGAGGTACCTCTTGGAGGC
075 rv_epo_check Sample Preparation GCCCGACCTCCATCCTCTTCCAG
076 rv_adKan150 Fusion Protein catcctatggaactgcctcggtgagttttctccttc
077 rv_adKan300 Fusion Protein gaaatgcataagcttttgccattctcaccggattc
078 rv_adKan800 Fusion Protein gagccatattcaacgggaaacgtcttgctcc
079 fw_3_dxCas9-L-Tn5_Gblock Fusion Protein agaaataattttgtttaactttaataaggagatataccatgggc
080 rv_3_dxCas9-L-Tn5_Gblock Fusion Protein CTCGAATCAGTTTGTCATTTTCATCGTACTTG
081 fw_4_dxCas9-L-Tn5_Gblock Fusion Protein CCCAAATTCTCGATTCACGCATG
082 rv_4_dxCas9-L-Tn5_Gblock Fusion Protein tttagaggccccaaggggttatg
083 fw_2pACYC-Duet_Backbone Fusion Protein CTGCTGCCACCGCTG
084 rv_2pACYC-Duet_Backbone Fusion Protein GCTGCTGCCCATGGTATATC
085 fw_gblock1_mut_EcoRI Fusion Protein CAGGATCCGAATACTATGATTACC
086 rv_gblock1_mut_EcoRI Fusion Protein GGTAATCATAGTATTCGGATCCTG
087 fw_adTn5_Phos Fusion Protein P-CTGTCTCTTATACACATCT
088 fw_1_Tn5-dxCas9_Gblock_option2 Fusion Protein accatgggcagcagccatcaccatcatcaccacagcc
089 rv_1_Tn5-dxCas9_Gblock_option2 Fusion Protein cagggaaaagattgagaaaatcctcacatttcggataccctac
090 rv_BB_dxCas9_2 BioBrick CTGCAGCGGCCGCTACTAGTAttatGGCCGGCCcaccttcctcttcttcttg
091 fw_big_bovine_alb Sample Preparation CCCCCGTATCTGTCATTTCTTT
092 rv_big_bovine_alb Sample Preparation GGCATGGCAACCCACTGC
093 fw_dxcas_BL_bb BioBrick P-TACTAGTAGCGGCCGCTGCAGTCCGG
094 rv_dxcas_BL_bb BioBrick P-ttacaccttcctcttcttcttggggtcagccct
095 EPO-2 d-AuNPs TCCTGTCCCTGCTGTCGCTC
096 EPO-3 d-AuNPs GCCTGGAAGAGGATGGAGGT
097 EPO-4 d-AuNPs CTGCATGTGGATAAAGCCGT
098 EPO-5 d-AuNPs CGAACAATCACTGCTGACAC
099
100
101 fw_IV1 In Vivo CCCTGAACCGACGACCGG
102 rv_IV1 In Vivo GCAGTGTGACCGTGTGCTTC
103 fw_IV2 In Vivo CTGAAACCTCAGGCATTTGAGAAGC
104 rv_IV2 In Vivo GAAATTCGAAAGCAAATTCGACCCGG
105 fw_KanR In Vivo CTAGCTGTCTCTTATACACATCTTTAGAAAAACTCATCGAGCATCAAATG
106 rv_KanR In Vivo GACTCTGTCTCTTATACACATCTATGAGCCATATTCAACGGG
107 fw_diagnostic In Vivo CCGGTAATAATCCACAGCAG
108 rv_diagnostic In Vivo CCAGATTCAGACAGCCAATC
109 fw_KanRCassetteME In Vivo CTAGCTGTCTCTTATACACATCTTAAcTAAcTAAcagctcgagtcccgtcaag
110 rv_KanRCassetteME In Vivo CTAGCTGTCTCTTATACACATCTTAAcTAAcTAAcaaagccacgttgtgtctc
111 fw_KanRCassette In Vivo TAAcTAAcTAAcagctcgagtcccgtc
112 rv_KanRCassette In Vivo TAAcTAAcTAAcaaagccacgttgtgtctc
113 fw_KanRCassetteME In Vivo cttggaCTGTCTCTTATACACATCTgacagctcgagtcccgtc
114 rv_KanRCassetteME In Vivo tgtcatCTGTCTCTTATACACATCTattcaacaaagccacgttgtgtctc
115 fw_KanRCassette In Vivo ctgacagctcgagtcccgtc
116 rv_KanRCassette In Vivo attcaacaaagccacgttgtgtctc
117 fw_KanRMEBiobrick BioBrick gaattcgcggccgcttctagagcttggaCTGTCTCTTATACACATCTgacag
118 rv_KanRMEBiobrick BioBrick tactagtagcggccgctgcagtgtcatCTGTCTCTTATACACATCTattcaacaaagc
119 rv_KanR internal BioBrick gcagtttcatttgatgctcgatgag
120 fw_KanR internal BioBrick ggctcataacaccccttgtattactg
121 fw target seq LacZ In Vivo TCGCACAGCGTGTACCACAG
122 rv target seq LacZ In Vivo CTGTGGTACACGCTGTGCG
123 fw_pSB1C3ori BioBrick GCAAGCAGCAGATTACGCG
124 SeqTim_01 Sequencing GATATGAATAAATTGCAGTTTGTCGGGCAGCAGGCCGTAGA
125 SeqTim_02 Sequencing CATCATTGGCAACGCTACCCTCCATCCTCTTCCAGGCAT
126 SeqTim_03 Sequencing TCTACGGCCTGCTGCCCGACAAACTGCAATTTATTCATATC
127 SeqTim_04 Sequencing ATGCCTGGAAGAGGATGGAGGGTAGCGTTGCCAATGATG
128 SeqTim_05 Sequencing TTCGGGCTCTGGGAGCCCAGccgcctccatccagtctatt
129 SeqTim_06 Sequencing GGAGGGGAGATGGCTTCCTTgaatgaagccataccaaacg
130 SeqTim_07 Sequencing cgtttggtatggcttcattcAAGGAAGCCATCTCCCCTCC
130 SeqTim_08 Sequencing aatagactggatggaggcggCTGGGCTCCCAGAGCCCGAA