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| − | <p>We are engineering E. coli to produce taxol from an intermediate (10-Deacetylbaccatin III) in the taxol synthesis pathway. We are using a modular approach to link the five necessary genes together onto a single DNA strand so that our design can be easily adapted for next generation taxanes in the future. Each gene in the pathway will be equipped with a T7 promoter of various strengths from a promoter library, fitted to the genes using the ePathOptimize approach for metabolic engineering. The project's end goal is to analyze the activity of produced taxol and evaluate this taxol biosynthesis design's feasibility in industrially relevant conditions. Homology modeling is used to develop protein models for the five necessary genes to determine active site architecture and catalytic functions. These models will then be considered when mutating the genes to produce next generation taxane products.</p>
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| − | <h1> Welcome to iGEM 2018! </h1> | + | <h1>Taxol Biosynthesis in E. Coli </h1> |
| − | <p>Your team has been approved and you are ready to start the iGEM season! </p>
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| − | <div class="column full_size" > | + | <h2> Project Overview </h2> |
| − | | + | <p> <b>Adam</b>: describe taxol</p> |
| − | <h3>Before you start</h3> | + | <br> <br> |
| − | <p> Please read the following pages:</p> | + | <p><b>Trudy</b>: describe the importance of our project (essentially what you've done for human practices)</p> |
| − | <ul> | + | <br> <br> |
| − | <li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li> | + | <p>The aim of our project was to produce taxol in E. Coli starting from an intermediate (10-Deacetylbaccatin III) found in the taxol synthesis pathway. Our design used a modular approach to link the five necessary genes together onto a single DNA strand so that it could be easily adapted for next generation taxanes in the future. The project's end goal is to analyze the activity of produced taxol and evaluate this taxol biosynthesis design's feasibility in industrially relevant conditions. Homology modeling was used to develop protein models for the five necessary genes to determine active site architecture and catalytic functions. These models were then used to consider possible mutations to the genes in order to produce alternative taxane products with enhanced characteristics such as increased solubility and decreased toxicity.</p> |
| − | <li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li> | + | |
| − | <li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li> | + | |
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | <p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Taxol Biosynthesis in E. Coli
Project Overview
Adam: describe taxol
Trudy: describe the importance of our project (essentially what you've done for human practices)
The aim of our project was to produce taxol in E. Coli starting from an intermediate (10-Deacetylbaccatin III) found in the taxol synthesis pathway. Our design used a modular approach to link the five necessary genes together onto a single DNA strand so that it could be easily adapted for next generation taxanes in the future. The project's end goal is to analyze the activity of produced taxol and evaluate this taxol biosynthesis design's feasibility in industrially relevant conditions. Homology modeling was used to develop protein models for the five necessary genes to determine active site architecture and catalytic functions. These models were then used to consider possible mutations to the genes in order to produce alternative taxane products with enhanced characteristics such as increased solubility and decreased toxicity.
