Difference between revisions of "Template:Virginia/Collaborations"
| Line 2: | Line 2: | ||
<figure> | <figure> | ||
<img src="https://static.igem.org/mediawiki/2018/7/73/T--Virginia--2018_Postcard_Front.png" alt="PostcardExchange"> | <img src="https://static.igem.org/mediawiki/2018/7/73/T--Virginia--2018_Postcard_Front.png" alt="PostcardExchange"> | ||
| − | <figcaption> | + | <figcaption> Our postcard design for the exchange. </figcaption> |
</figure> | </figure> | ||
| + | <h2 id="postcard-exchange">Postcard Exchange</h2> | ||
| + | <p>This year, <a href="https://2018.igem.org/Team:Duesseldorf/Collaborations" target="_blank">Team Düesseldorf</a> held a postcard exchange event for iGEM teams in order to promote synthetic biology within various communities. Each iGEM team that participated designed a different postcard and exchanged their postcard with other participating teams so that every team could receive postcards from all over the world. </p> | ||
| + | <p>This year, we participated in the event and exchanged postcards with 50 other participating schools! The word cloud on our postcard was generated from the most frequency used words in all 2018 iGEM project descrptions in the shape of the University of Virginia Rotunda, the icon of our university.</p> | ||
<figure> | <figure> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/1/1b/T--Virginia--2018_MAM.jpg" alt="MAM"> |
| − | <figcaption> | + | <figcaption>The team posing with Professor Kozminski at the Mid-Atlantic Meetup. </figcaption> |
</figure> | </figure> | ||
| − | + | <h2 id="mid-atlantic-meetup">Mid-Atlantic Meetup</h2> | |
| − | <h2 id=" | + | <p>We participated in the hosted by the University of Maryland iGEM team! We had the opportunity to listen to other teams talk about their project, get feedback about our own project, and start collaborations.</p> |
| − | <p> | + | <p>We participated in the <a href="https://2018.igem.org/Team:UMaryland/meetup" target="_blank">Mid-Atlantic Meetup</a> hosted by the University of Maryland. We gave a presentation on our project and the progress that we’ve made. Other teams that presented include William and Mary, Pittsburgh, Richard Montgomery, and Georgia State. </p> |
| − | <p> | + | <p>During the presentations, we found out that it was the first time for Richard Montgomery High School to have an iGEM team, and that they were also working on a project related to quorum sensing. After more discussion after the presentations, we decided to mentor the RMHS high school team and help them with not only wetlab techniques, but also modeling their project, building a wiki page, and human practice. </p> |
| + | <p>Thank you to UMaryland for holding such a successful event, we got lots of feedbacks for our project, and started many collaborations with other participating teams during the meetup! </p> | ||
| + | <h2 id="water-quality-test">Water Quality Test</h2> | ||
| + | <p><a href="https://2018.igem.org/Team:Exeter/Collaborations" target="_blank">Team Exeter</a> is creating a bioreactor that can break down perchlorate this year. They were looking for water quality reports from US local councils or water purification plants for a water assay of the drinking water, specifically testing for perchlorate. </p> | ||
| + | <p>Levels of perchlorate that cause health risks are typically associated with areas containing sites involved in the manufacture, maintenance, use and disposal of ammunition and rocket fuel. Virginia is currently not required to test for perchlorate because we are not at the risk for this kind of contamination. We went through Virginia’s Department of Health, got in contact with many people, and got a report from Fairfax County when they measured the perchlorate level in drinking water back in 2013. </p> | ||
| + | <p>Providing the water quality report gave them a better idea of what test is performed on drinking water in the US. We also did a perchlorate assay on our tap water. We compared tap water under the microscope with DI water as our negative control, and three different concentrations of sodium perchlorate solutions as the positive control. </p> | ||
| + | <p>After doing the water quality test, we had a Skype meeting with members from Team Exeter and talked about how the protocol may be revised so that a more accurate result can be produced. We suggested that the amount of perchlorate added in the water and the size of the droplet we put on the microscope slides may need to be adjusted. We did a second run of the water quality test after they revised their protocol, and this time we got a much more desired result! </p> | ||
| + | <h2 id="richard-montgomery-high-school-rmhs-">Richard Montgomery High School (RMHS)</h2> | ||
<figure> | <figure> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/4/4e/T--Virginia--2018_MAM1.jpg" alt="MAM1"> |
| − | <figcaption> | + | <figcaption> We are thankful to have the opportunity to collaborate with the Richard Montgomery High School iGEM team! </figcaption> |
</figure> | </figure> | ||
| + | <p>We were fortunate enough to collaborate with and mentor <a href="https://2018.igem.org/Team:RMHS_Maryland" target="_blank">RMHS</a>, a high school team who is also working with quorum sensing. Since they are in close collaboration with the University of Maryland, they were able to give us PCT6, which is an integral part of our project. As mentors, we helped RMHS with various aspects of their project, including helping edit their wiki, develop wetlab techniques, design events for human practices, and build a model. </p> | ||
| + | <h3 id="rmhs-human-practices-collaboration">RMHS Human Practices Collaboration</h3> | ||
| + | <p>For public outreach and education, we collaborated with RMHS to design a one-day activity package for elementary school kids. Since both our teams’ project were related to quorum sensing, this one-day activity is aimed to let participants learn more about bacterias and even quorum sensing. Below is the schedule for the event: </p> | ||
| + | <table style="width:100%" id="parts"> | ||
| + | <pre><code><tr> | ||
| + | <th>Description</th> | ||
| + | <th>Time</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><b>Brief Introduction to Quorum Sensing:</b> <br> Quorum Sensing Video by Virginia iGEM</td> | ||
| + | <td>4 minutes</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><b>How do Bacterias Communicate?</b> <br>Lecture on How bacterias communicate with each other in a population</td> | ||
| + | <td>10 minutes </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><b>Making a 3D Model of Bacteria!*</b> <br>Learn about the structure of a bacteria and make a model out of some common materials!</td> | ||
| + | <td>30 minutes</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><b>Brainstorming Time!</b> <br>How can bacteria be beneficial in our life?</td> | ||
| + | <td>30 minutes </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><b>Q&A</b></td> | ||
| + | <td>10 minutes</td> | ||
| + | </tr></code></pre> | ||
| + | </table> | ||
| − | + | <p>We were planning to host the event in collaboration with <a href="https://kid-museum.org/">Kid Museum</a> since they host weekend workshops. However, due to time conflicts and reservation for the physical space, the event was not executed. We then developed our plan into a ready-to-use packet for one-day activities so future teams can use it as a public outreach and education event. The one-day event will be an opportunity to introduce the concept of quorum sensing and bacteria to kids through a relaxing and fun environment. </p> | |
| − | <p>We | + | <p>*Here is a list of materials needed for making the 3D model of a bacteria:<br>• Cell Wall + Capsule: An oval block with a base<br>• Pili and Flagella: Pipe cleaners<br>• Cytoplasm: Play Doh<br>• Ribosomes: Mini pompoms<br>• Plasmid: Circular block<br>• Chromosomal DNA Ring: String </p> |
| − | <p> | + | <h3 id="wetlab-collaboration">Wetlab Collaboration</h3> |
| − | < | + | <p>In regards to wetlab, we reached out to RMHS over email and video call to give advice on which cloning techniques would be best for their project. Additionally, we directed them to and instructed them on how to use resources, such as IDT and NEB, to obtain materials for these techniques. </p> |
| − | + | <h2 id="wiki">Wiki</h2> | |
| − | <h2 id=" | + | <p>See our <a href="https://2018.igem.org/Team:Virginia/Wiki">Wiki</a> page for more details.</p> |
| + | <h2 id="william-and-mary">William and Mary</h2> | ||
| + | <p>During the summer, we completed a reciprocal collaboration with iGEM at the <a href="https://2018.igem.org/Team:William_and_Mary" target="_blank">College of William and Mary</a>. First, we tested two experiments for W&M’s 3G assembly, which combines golden gate and gibson assembly into a single multi-step cloning and assembly technique. The W&M team sent us a kit containing most of the parts and reagents necessary for the completion of the experiments as well as the protocols. We completed the experiments and gave feedback on the clarity and ease of completing each protocol. In addition, we provided fluorescence data for the cells that were transformed with the constructs, which helped to characterize and troubleshoot the system. </p> | ||
<figure> | <figure> | ||
| − | <img src="https:// | + | <img src="https://2018.igem.org" alt="W&M"> |
| − | <figcaption> | + | <figcaption> Our labwork done in collaboration with William and Mary iGEM. These are the plates for testing the final constructs of the 2 experiments. </figcaption> |
| − | </figure> | + | </figure> |
| − | + | <p>Next, we sent the William and Mary team each of our basic parts for them to adapt into their 3G system. Since our project was designed with modular assembly and testing in mind, having the parts on W&M’s system will allow future teams to more easily reproduce our results, as well as have the building blocks to improve upon it further. </p> | |
| − | + | <p>Adapted Parts (should have links to our parts and to theirs, but some were probably biobricks before):<br>• pLsrR<br>• pLsrA<br>• LsrR<br>• LsrK (was already a part in the registry) </p> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | <p>Next, we sent the William and Mary team each of our basic parts for them to adapt into their 3G system. Since our project was designed with modular testing in mind, having the parts on W&M’s system will allow future | + | |
Revision as of 19:35, 13 October 2018
Contents
Collaborations
<figure>
<img src="" alt="PostcardExchange"> <figcaption> Our postcard design for the exchange. </figcaption>
</figure>
Postcard Exchange
This year, <a href="https://2018.igem.org/Team:Duesseldorf/Collaborations" target="_blank">Team Düesseldorf</a> held a postcard exchange event for iGEM teams in order to promote synthetic biology within various communities. Each iGEM team that participated designed a different postcard and exchanged their postcard with other participating teams so that every team could receive postcards from all over the world.
This year, we participated in the event and exchanged postcards with 50 other participating schools! The word cloud on our postcard was generated from the most frequency used words in all 2018 iGEM project descrptions in the shape of the University of Virginia Rotunda, the icon of our university.
<figure>
<img src="" alt="MAM"> <figcaption>The team posing with Professor Kozminski at the Mid-Atlantic Meetup. </figcaption>
</figure>
Mid-Atlantic Meetup
We participated in the hosted by the University of Maryland iGEM team! We had the opportunity to listen to other teams talk about their project, get feedback about our own project, and start collaborations.
We participated in the <a href="https://2018.igem.org/Team:UMaryland/meetup" target="_blank">Mid-Atlantic Meetup</a> hosted by the University of Maryland. We gave a presentation on our project and the progress that we’ve made. Other teams that presented include William and Mary, Pittsburgh, Richard Montgomery, and Georgia State.
During the presentations, we found out that it was the first time for Richard Montgomery High School to have an iGEM team, and that they were also working on a project related to quorum sensing. After more discussion after the presentations, we decided to mentor the RMHS high school team and help them with not only wetlab techniques, but also modeling their project, building a wiki page, and human practice.
Thank you to UMaryland for holding such a successful event, we got lots of feedbacks for our project, and started many collaborations with other participating teams during the meetup!
Water Quality Test
<a href="https://2018.igem.org/Team:Exeter/Collaborations" target="_blank">Team Exeter</a> is creating a bioreactor that can break down perchlorate this year. They were looking for water quality reports from US local councils or water purification plants for a water assay of the drinking water, specifically testing for perchlorate.
Levels of perchlorate that cause health risks are typically associated with areas containing sites involved in the manufacture, maintenance, use and disposal of ammunition and rocket fuel. Virginia is currently not required to test for perchlorate because we are not at the risk for this kind of contamination. We went through Virginia’s Department of Health, got in contact with many people, and got a report from Fairfax County when they measured the perchlorate level in drinking water back in 2013.
Providing the water quality report gave them a better idea of what test is performed on drinking water in the US. We also did a perchlorate assay on our tap water. We compared tap water under the microscope with DI water as our negative control, and three different concentrations of sodium perchlorate solutions as the positive control.
After doing the water quality test, we had a Skype meeting with members from Team Exeter and talked about how the protocol may be revised so that a more accurate result can be produced. We suggested that the amount of perchlorate added in the water and the size of the droplet we put on the microscope slides may need to be adjusted. We did a second run of the water quality test after they revised their protocol, and this time we got a much more desired result!
Richard Montgomery High School (RMHS)
<figure>
<img src="" alt="MAM1"> <figcaption> We are thankful to have the opportunity to collaborate with the Richard Montgomery High School iGEM team! </figcaption>
</figure>
We were fortunate enough to collaborate with and mentor <a href="https://2018.igem.org/Team:RMHS_Maryland" target="_blank">RMHS</a>, a high school team who is also working with quorum sensing. Since they are in close collaboration with the University of Maryland, they were able to give us PCT6, which is an integral part of our project. As mentors, we helped RMHS with various aspects of their project, including helping edit their wiki, develop wetlab techniques, design events for human practices, and build a model.
RMHS Human Practices Collaboration
For public outreach and education, we collaborated with RMHS to design a one-day activity package for elementary school kids. Since both our teams’ project were related to quorum sensing, this one-day activity is aimed to let participants learn more about bacterias and even quorum sensing. Below is the schedule for the event:
We were planning to host the event in collaboration with <a href="https://kid-museum.org/">Kid Museum</a> since they host weekend workshops. However, due to time conflicts and reservation for the physical space, the event was not executed. We then developed our plan into a ready-to-use packet for one-day activities so future teams can use it as a public outreach and education event. The one-day event will be an opportunity to introduce the concept of quorum sensing and bacteria to kids through a relaxing and fun environment.
*Here is a list of materials needed for making the 3D model of a bacteria:
• Cell Wall + Capsule: An oval block with a base
• Pili and Flagella: Pipe cleaners
• Cytoplasm: Play Doh
• Ribosomes: Mini pompoms
• Plasmid: Circular block
• Chromosomal DNA Ring: String
Wetlab Collaboration
In regards to wetlab, we reached out to RMHS over email and video call to give advice on which cloning techniques would be best for their project. Additionally, we directed them to and instructed them on how to use resources, such as IDT and NEB, to obtain materials for these techniques.
Wiki
See our <a href="https://2018.igem.org/Team:Virginia/Wiki">Wiki</a> page for more details.
William and Mary
During the summer, we completed a reciprocal collaboration with iGEM at the <a href="https://2018.igem.org/Team:William_and_Mary" target="_blank">College of William and Mary</a>. First, we tested two experiments for W&M’s 3G assembly, which combines golden gate and gibson assembly into a single multi-step cloning and assembly technique. The W&M team sent us a kit containing most of the parts and reagents necessary for the completion of the experiments as well as the protocols. We completed the experiments and gave feedback on the clarity and ease of completing each protocol. In addition, we provided fluorescence data for the cells that were transformed with the constructs, which helped to characterize and troubleshoot the system.
<figure>
<img src="https://2018.igem.org" alt="W&M"> <figcaption> Our labwork done in collaboration with William and Mary iGEM. These are the plates for testing the final constructs of the 2 experiments. </figcaption>
</figure>
Next, we sent the William and Mary team each of our basic parts for them to adapt into their 3G system. Since our project was designed with modular assembly and testing in mind, having the parts on W&M’s system will allow future teams to more easily reproduce our results, as well as have the building blocks to improve upon it further.
Adapted Parts (should have links to our parts and to theirs, but some were probably biobricks before):
• pLsrR
• pLsrA
• LsrR
• LsrK (was already a part in the registry)