Difference between revisions of "Team:Newcastle/Results/Chemotaxis/NaringeninMIC"

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                 <p class="about-para">Our selected flavonoid, naringenin, is known to possess antimicrobial properties in both gram-positive and gram-negative bacteria [1]. As such, we decided to characterise the effect of naringenin on the growth rate of our bacteria: Azospirillum brasilense, Azorhizobium caulinodans, Herbaspirillum seropedicae, and Escherichia coli. This was done so that we would understand which concentrations of naringenin to use in our chemotaxis assays.</p>
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                 <p class="about-para">In addition to being a chemoattractant, naringenin possess antimicrobial properties against both Gram-positive and Gram-negative bacteria [1]. We therefore characterised the effect of increasing concentrations of naringenin on the growth rate of <i>Azospirillum brasilense</i>, Azorhizobium caulinodans, Herbaspirillum seropedicae, and Escherichia coli. This was done so that we would understand which concentrations of naringenin to use in our chemotaxis assays.</p>
 
              
 
              
 
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Revision as of 20:29, 13 October 2018

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Influence of Naringenin on Growth Rate of Bacteria in Liquid Media

Principle Results Graphical Overview
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Principle

In addition to being a chemoattractant, naringenin possess antimicrobial properties against both Gram-positive and Gram-negative bacteria [1]. We therefore characterised the effect of increasing concentrations of naringenin on the growth rate of Azospirillum brasilense, Azorhizobium caulinodans, Herbaspirillum seropedicae, and Escherichia coli. This was done so that we would understand which concentrations of naringenin to use in our chemotaxis assays.

Graphical Overview

INSERT MIC GRAPH HERE

Results

All species successfully grew in the presence of 0-150μM naringenin (Figure 1). However, it was noted that E. coli showed reduced growth rate even at lower concentration of naringenin. When concentration of naringenin exceeded 100μM, there exists greater flux in all species suggesting that naringenin begins to have a greater impact on some, but not all, bacteria. As such, naringenin concentrations of <100μM were used as part of subsequent chemotaxis assays as to not negatively impact bacterial growth.


REFERENCES & Attributions

1. 1. Céliz G, Daz M, & Audisio MC (2011) Antibacterial Activity of Naringin Derivatives Against Pathogenic Strains. Journal of Applied Microbiology 111(3):731-738.

Attributions:

Primary Author: Connor Trotter

Stylisation: Connor Trotter, Umar Farooq

Troubleshooting: Umar Farooq