Line 23: | Line 23: | ||
<dd>• cPCR, chloramphenicol, and culturing protocols, made agarose gel </dd> | <dd>• cPCR, chloramphenicol, and culturing protocols, made agarose gel </dd> | ||
<dd>• gel electrophoresis for cPCR, nucleic acid purification plasmid </dd> | <dd>• gel electrophoresis for cPCR, nucleic acid purification plasmid </dd> | ||
+ | <dt><b><font color="#009999">Week 3 (June 18-22)</font></b></dt> | ||
+ | <dd>•Made chloramphenicol plates</dd> | ||
+ | <dd>•Restriction enzyme digest of iGem plasmid using EcoRI and PstI</dd> | ||
</dl> | </dl> | ||
Revision as of 16:07, 18 June 2018
Notebook
- Week 1 (June 4-8)
- •Made competent cells
- Week 2 (June 11-15)
- •Testing competent cells using iGem DNA transformation samples.
- • Tested to see if they grew better on a freezer block versus an ice bath.
- • Ran colony PCR and plated bacteria on antimicrobial plate.
- • cPCR, chloramphenicol, and culturing protocols, made agarose gel
- • gel electrophoresis for cPCR, nucleic acid purification plasmid
- Week 3 (June 18-22)
- •Made chloramphenicol plates
- •Restriction enzyme digest of iGem plasmid using EcoRI and PstI